FastQCFastQC Report
Wed 25 May 2016
SRR2049456_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049456_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7316380
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81900.052.8815731
GTATCAA151950.037.106251
ATCAACG161950.033.6943553
TCAACGC165800.032.882924
TATCAAC186600.029.87282
CAACGCA191450.028.5517795
AACGCAG209000.026.244746
CGCAGAG203700.026.0944238
ACGCAGA215250.025.0248267
GTGGTAT44500.023.7766761
GTACATG239850.023.5664061
TGGTATC40300.023.3253332
TACATGG239500.022.5877362
GCAGAGT229550.022.2558029
ACATGGG247550.021.1888373
GCGGGTA4850.020.3501959
CAGAGTA233250.018.98105210-11
TCTGTCG18800.018.2490888
CATGGGA107100.018.1241444
AGAGTAC218400.017.8076410-11
Produced by FastQC (version 0.11.5)