##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049456_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7316380 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.05351088926491 31.0 31.0 34.0 28.0 34.0 2 31.28682326505731 31.0 31.0 34.0 28.0 34.0 3 31.36340731345283 33.0 31.0 34.0 28.0 34.0 4 34.94350648818131 37.0 35.0 37.0 32.0 37.0 5 34.93094576826245 37.0 35.0 37.0 32.0 37.0 6 35.01614787640882 37.0 35.0 37.0 32.0 37.0 7 34.92016338681151 37.0 35.0 37.0 32.0 37.0 8 34.9083863878038 37.0 35.0 37.0 32.0 37.0 9 36.45724647981652 39.0 37.0 39.0 32.0 39.0 10-11 36.39955722638791 39.0 37.0 39.0 32.0 39.0 12-13 36.312969528646676 39.0 37.0 39.0 32.0 39.0 14-15 37.55746304866614 40.0 38.0 41.0 32.0 41.0 16-17 37.563393577151544 40.0 38.0 41.0 32.0 41.0 18-19 37.60902563836214 40.0 38.0 41.0 32.0 41.0 20-21 37.676549400113174 40.0 38.0 41.0 32.0 41.0 22-23 37.65397669339209 40.0 38.0 41.0 32.0 41.0 24-25 37.675973773915516 40.0 38.0 41.0 32.0 41.0 26-27 37.68889259442511 40.0 38.0 41.0 32.0 41.0 28-29 37.683125261399766 40.0 38.0 41.0 32.0 41.0 30-31 37.62790129818298 40.0 38.0 41.0 32.0 41.0 32-33 37.573865080818656 40.0 38.0 41.0 32.0 41.0 34-35 37.500227844917845 40.0 38.0 41.0 32.0 41.0 36-37 37.38394581746711 40.0 38.0 41.0 31.5 41.0 38-39 37.278433186903904 40.0 37.5 41.0 31.0 41.0 40-41 37.15694633138246 40.0 37.0 41.0 31.0 41.0 42-43 37.06179442565859 40.0 37.0 41.0 31.0 41.0 44-45 36.92058723029695 40.0 37.0 41.0 30.0 41.0 46-47 36.80112699176368 40.0 37.0 41.0 30.0 41.0 48-49 36.69782645242593 40.0 37.0 41.0 30.0 41.0 50-51 36.59016624341545 40.0 36.0 41.0 30.0 41.0 52-53 36.47914255410463 40.0 36.0 41.0 30.0 41.0 54-55 36.340732712078925 40.0 36.0 41.0 30.0 41.0 56-57 36.19126514751831 39.5 35.5 41.0 29.5 41.0 58-59 36.01967988267422 39.0 35.0 41.0 29.0 41.0 60-61 35.83974397174559 39.0 35.0 41.0 29.0 41.0 62-63 35.60119929527991 39.0 35.0 41.0 28.5 41.0 64-65 35.374872887958254 38.5 35.0 40.0 28.0 41.0 66-67 35.158910349107074 38.0 35.0 40.0 28.0 41.0 68-69 34.8778201104918 37.5 34.0 40.0 28.0 41.0 70-71 34.568530338774096 37.0 34.0 40.0 28.0 41.0 72-73 34.23005632566925 36.5 34.0 39.0 27.0 41.0 74-75 33.91669808019813 36.0 34.0 39.0 27.0 41.0 76-77 33.581945784664 36.0 34.0 39.0 26.0 40.0 78-79 33.21742938447703 35.0 34.0 37.5 26.0 39.5 80-81 32.863989158572956 35.0 33.0 37.0 26.0 39.0 82-83 32.54162352146827 35.0 33.0 37.0 26.0 39.0 84-85 32.226447163761314 35.0 33.0 36.0 25.0 38.0 86-87 31.931735708096078 35.0 33.0 36.0 25.0 37.0 88-89 31.676485570733067 35.0 33.0 36.0 24.5 37.0 90-91 31.438097529105924 35.0 33.0 35.0 24.0 36.5 92-93 31.20913696390838 35.0 33.0 35.0 23.0 36.0 94-95 30.981431937652225 35.0 32.0 35.0 20.0 36.0 96-97 30.77896398218791 35.0 32.0 35.0 19.0 36.0 98-99 30.590604711619683 35.0 32.0 35.0 9.5 35.5 100 30.449183886020137 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29634335491532937 1101 2 0.3754609465849512 1101 3 0.34915542069488836 1101 4 0.40041473964946306 1101 5 0.38239142913805324 1101 6 0.4262940281388339 1101 7 0.4650378467125762 1101 8 0.4690360633699058 1101 9 0.5777938285671382 1101 10-11 0.6309638430806785 1101 12-13 0.6238430986441301 1101 14-15 0.704453784543368 1101 16-17 0.6459560630575965 1101 18-19 0.6298679220090548 1101 20-21 0.638046802927704 1101 22-23 0.6081376370634217 1101 24-25 0.6387861575585134 1101 26-27 0.6365583994380586 1101 28-29 0.5839558871982362 1101 30-31 0.6197188606814876 1101 32-33 0.6508762447349952 1101 34-35 0.6796996657290535 1101 36-37 0.6218061471653087 1101 38-39 0.6135072927497163 1101 40-41 0.6607920878657367 1101 42-43 0.6019876367051609 1101 44-45 0.622730231416746 1101 46-47 0.5704734741746051 1101 48-49 0.5796900165751282 1101 50-51 0.5836046442152778 1101 52-53 0.5385719783638763 1101 54-55 0.4747467242409229 1101 56-57 0.4965420491781174 1101 58-59 0.5008195146147969 1101 60-61 0.4697201001870539 1101 62-63 0.45569291691290204 1101 64-65 0.4625909095097924 1101 66-67 0.46465038760457134 1101 68-69 0.45382525360621173 1101 70-71 0.4559361750437674 1101 72-73 0.4767119444465422 1101 74-75 0.4592944618710817 1101 76-77 0.40235252558456835 1101 78-79 0.40791388206833545 1101 80-81 0.4608669691364611 1101 82-83 0.4411215285070469 1101 84-85 0.4478972818374771 1101 86-87 0.4073076133862159 1101 88-89 0.40694542492903807 1101 90-91 0.42964576673288946 1101 92-93 0.44131281152737145 1101 94-95 0.4628422206265945 1101 96-97 0.4338191390196364 1101 98-99 0.5142204140165845 1101 100 0.4925492757317578 1102 1 0.19276656230292133 1102 2 0.28391911960993355 1102 3 0.33329189153721117 1102 4 0.3368470831125947 1102 5 0.29764124522402824 1102 6 0.3195133477338885 1102 7 0.3319348402107636 1102 8 0.39607948685772953 1102 9 0.45646667133932795 1102 10-11 0.5029220881059331 1102 12-13 0.4578977250356999 1102 14-15 0.5633604401976626 1102 16-17 0.46521264796231065 1102 18-19 0.4975403132128946 1102 20-21 0.5202996906198223 1102 22-23 0.5253585471690556 1102 24-25 0.5266164353357183 1102 26-27 0.4338696612905366 1102 28-29 0.47058017762009285 1102 30-31 0.4321898259044872 1102 32-33 0.43350859474504944 1102 34-35 0.44969565349213525 1102 36-37 0.4927864788488492 1102 38-39 0.5443487979683965 1102 40-41 0.5343102717814574 1102 42-43 0.48607988674193336 1102 44-45 0.42063532760281674 1102 46-47 0.4164844872810818 1102 48-49 0.40619714777495375 1102 50-51 0.4463732008639383 1102 52-53 0.4317103181460311 1102 54-55 0.37132096561639827 1102 56-57 0.3833062258411317 1102 58-59 0.399864615976 1102 60-61 0.38435128507776994 1102 62-63 0.37673144903599365 1102 64-65 0.38795929415105945 1102 66-67 0.42452247091272 1102 68-69 0.38539539733864103 1102 70-71 0.2994334135702772 1102 72-73 0.3647092533339489 1102 74-75 0.39313847623179043 1102 76-77 0.32996609180004555 1102 78-79 0.35827330435487426 1102 80-81 0.3501050677137414 1102 82-83 0.3241637380115989 1102 84-85 0.3310863792988741 1102 86-87 0.27914799796021583 1102 88-89 0.2878932911799801 1102 90-91 0.294349266548938 1102 92-93 0.24794791018678097 1102 94-95 0.22573762255654017 1102 96-97 0.2644170966445536 1102 98-99 0.27494802342619096 1102 100 0.20191221426510353 1103 1 0.292614023152165 1103 2 0.3445063424747268 1103 3 0.36045460820830755 1103 4 0.346410548951269 1103 5 0.3172364367600835 1103 6 0.3808502683383779 1103 7 0.42836413964431586 1103 8 0.43427403830108346 1103 9 0.551441608969192 1103 10-11 0.49807506388754774 1103 12-13 0.5210793997094783 1103 14-15 0.5913208252630042 1103 16-17 0.5815214987303179 1103 18-19 0.5250710559260483 1103 20-21 0.486569798871443 1103 22-23 0.5250149507664617 1103 24-25 0.547282238500479 1103 26-27 0.5365772876968293 1103 28-29 0.5081263646247933 1103 30-31 0.532170896699256 1103 32-33 0.5469828090798643 1103 34-35 0.5366495964525058 1103 36-37 0.5515446265929853 1103 38-39 0.5683839635156502 1103 40-41 0.6093571452108293 1103 42-43 0.5446409707359408 1103 44-45 0.4944257614801373 1103 46-47 0.46661906266427877 1103 48-49 0.4732204811531773 1103 50-51 0.4812072159227796 1103 52-53 0.4749981030039905 1103 54-55 0.41535728848393205 1103 56-57 0.42564441766043615 1103 58-59 0.4210758989510808 1103 60-61 0.37949023866804765 1103 62-63 0.40370720817649897 1103 64-65 0.42745706594803323 1103 66-67 0.3600491280021245 1103 68-69 0.35956450267674 1103 70-71 0.34745011091255407 1103 72-73 0.3760265624504271 1103 74-75 0.3516085409920322 1103 76-77 0.3081390975665528 1103 78-79 0.3576741305466058 1103 80-81 0.3522693650819164 1103 82-83 0.31615693120917143 1103 84-85 0.35008756309714073 1103 86-87 0.3515419059250533 1103 88-89 0.30225480146668104 1103 90-91 0.28849853357390387 1103 92-93 0.2783035897722108 1103 94-95 0.28197057094683586 1103 96-97 0.27807350608579284 1103 98-99 0.31536387975027225 1103 100 0.2548831732073751 1104 1 0.23552563776077307 1104 2 0.2840652002086088 1104 3 0.2732678967435547 1104 4 0.3391324983233588 1104 5 0.2857849645133612 1104 6 0.3573484903381612 1104 7 0.3858203582070985 1104 8 0.3659059951889603 1104 9 0.4586540157533818 1104 10-11 0.5039207663054128 1104 12-13 0.5104787438803982 1104 14-15 0.5657023078009615 1104 16-17 0.524561696286149 1104 18-19 0.5288109700618975 1104 20-21 0.5096234551685725 1104 22-23 0.5012330122685142 1104 24-25 0.5310592297095269 1104 26-27 0.5199583262089931 1104 28-29 0.5132060593208507 1104 30-31 0.505452590477141 1104 32-33 0.5112103634439933 1104 34-35 0.5787022275352527 1104 36-37 0.5485556012272923 1104 38-39 0.5709330590056823 1104 40-41 0.6031139810752322 1104 42-43 0.5924616112207204 1104 44-45 0.5740620799843654 1104 46-47 0.545831202596105 1104 48-49 0.5142129891665022 1104 50-51 0.5257113160330036 1104 52-53 0.5013915960831312 1104 54-55 0.4796894338010915 1104 56-57 0.4984281719272303 1104 58-59 0.5441880707649105 1104 60-61 0.5466703891976223 1104 62-63 0.5740954938977794 1104 64-65 0.5789008166069749 1104 66-67 0.524840081764232 1104 68-69 0.5369234586665641 1104 70-71 0.5497563575230018 1104 72-73 0.5196429932671975 1104 74-75 0.5565697876122471 1104 76-77 0.5444758192696497 1104 78-79 0.5459767068734536 1104 80-81 0.5190200051654443 1104 82-83 0.5449444671684205 1104 84-85 0.5192565024929152 1104 86-87 0.4850294870572043 1104 88-89 0.4203199021778694 1104 90-91 0.48394725773558633 1104 92-93 0.4371092351312704 1104 94-95 0.5295848664847433 1104 96-97 0.5353728698366176 1104 98-99 0.5092760039192896 1104 100 0.4707422234175844 1105 1 0.11913842311922451 1105 2 0.12401771005431073 1105 3 0.1544187454617827 1105 4 0.11713401567391202 1105 5 0.12029121048663427 1105 6 0.1381357961003289 1105 7 0.18021756229417463 1105 8 0.18625074479462 1105 9 0.19766695755545527 1105 10-11 0.23474886578516418 1105 12-13 0.22765885376672657 1105 14-15 0.23170756525261993 1105 16-17 0.14002539454737928 1105 18-19 0.14450573048287652 1105 20-21 0.20596482267855265 1105 22-23 0.18534987346177445 1105 24-25 0.18564669505435205 1105 26-27 0.19149676525893256 1105 28-29 0.19833524743993536 1105 30-31 0.2091248623216302 1105 32-33 0.2170959493764002 1105 34-35 0.21370709194359705 1105 36-37 0.1650616766533659 1105 38-39 0.1825267147826395 1105 40-41 0.15960744949344274 1105 42-43 0.13718429739100202 1105 44-45 0.1294194283081751 1105 46-47 0.08729728564429706 1105 48-49 0.0833024815691985 1105 50-51 0.0840099955688558 1105 52-53 0.05938565600056478 1105 54-55 0.024619141939901112 1105 56-57 0.010387780205853403 1105 58-59 0.002859735249181483 1105 60-61 0.021400652725652947 1105 62-63 0.0417444151453239 1105 64-65 0.07237484419229645 1105 66-67 0.038891145230444124 1105 68-69 -7.998843812231371E-4 1105 70-71 0.032470838306274175 1105 72-73 0.035636062065584895 1105 74-75 0.029418353529180763 1105 76-77 -0.0016296476466806098 1105 78-79 -0.005896112446329482 1105 80-81 0.012156848344588411 1105 82-83 -0.011687177084496625 1105 84-85 -0.04813638795488373 1105 86-87 -0.04194228113699339 1105 88-89 -0.05053548319523671 1105 90-91 -0.019185096283251823 1105 92-93 -0.028507295191591453 1105 94-95 8.13483823698391E-5 1105 96-97 -0.07960267438080848 1105 98-99 -0.06051594563410845 1105 100 -0.0914701716165851 1106 1 0.16722679904998117 1106 2 0.23441874777483562 1106 3 0.20467617967249296 1106 4 0.20110242717025528 1106 5 0.19616962296710483 1106 6 0.21134784334422108 1106 7 0.27555871515625086 1106 8 0.2779178758586127 1106 9 0.3248650818381904 1106 10-11 0.262637670753179 1106 12-13 0.2791299129865408 1106 14-15 0.3486603467818057 1106 16-17 0.3338426918129187 1106 18-19 0.32956452054932583 1106 20-21 0.3303987091650811 1106 22-23 0.35183394305260407 1106 24-25 0.3607154357392304 1106 26-27 0.3552775549662499 1106 28-29 0.30813417591392067 1106 30-31 0.3270002070131355 1106 32-33 0.32144863681407543 1106 34-35 0.33336722424348153 1106 36-37 0.2947328056184091 1106 38-39 0.2650821727159496 1106 40-41 0.3172932779183313 1106 42-43 0.27917132245910636 1106 44-45 0.2504711716557324 1106 46-47 0.28031182767014684 1106 48-49 0.22752572796867554 1106 50-51 0.2580890890863543 1106 52-53 0.23030805975274404 1106 54-55 0.235230482483054 1106 56-57 0.17507361113387532 1106 58-59 0.2056998970676034 1106 60-61 0.23302962284520135 1106 62-63 0.25643353308419137 1106 64-65 0.2506866064345701 1106 66-67 0.27746128628735534 1106 68-69 0.23174688504043672 1106 70-71 0.2328314477694633 1106 72-73 0.2138006136494468 1106 74-75 0.22581788436272632 1106 76-77 0.16376205404081645 1106 78-79 0.09373405601593987 1106 80-81 0.16009315226622078 1106 82-83 0.16146042103277125 1106 84-85 0.17794307233769047 1106 86-87 0.19092622011648785 1106 88-89 0.20195598539773485 1106 90-91 0.1760808176249924 1106 92-93 0.05785407338008142 1106 94-95 0.15635509275410442 1106 96-97 0.2394336899717615 1106 98-99 0.25352648091503127 1106 100 0.25165982419527566 1107 1 0.17840914518446027 1107 2 0.1922536129447927 1107 3 0.21739269756818658 1107 4 0.16175944579767787 1107 5 0.17222494297512725 1107 6 0.19845181977699866 1107 7 0.20989196813219024 1107 8 0.20484919494491294 1107 9 0.22990593975283957 1107 10-11 0.25047764999791156 1107 12-13 0.25736096756905624 1107 14-15 0.3010427577933754 1107 16-17 0.2969493188996495 1107 18-19 0.26774839609240075 1107 20-21 0.2671522948341547 1107 22-23 0.2668311668586796 1107 24-25 0.2971514766776693 1107 26-27 0.36828306812550693 1107 28-29 0.3376081373093456 1107 30-31 0.3076041441539701 1107 32-33 0.29829013339056587 1107 34-35 0.3132142732500469 1107 36-37 0.33657464444225127 1107 38-39 0.3137789535175699 1107 40-41 0.3742096407322677 1107 42-43 0.37337535415394996 1107 44-45 0.28212370103674544 1107 46-47 0.23135352006870136 1107 48-49 0.3027708824239923 1107 50-51 0.30786756574405416 1107 52-53 0.2730367604716051 1107 54-55 0.2312996291994054 1107 56-57 0.27148259827470156 1107 58-59 0.27342265332377025 1107 60-61 0.26518646984222727 1107 62-63 0.2695178285843909 1107 64-65 0.3109511110607741 1107 66-67 0.3226709952343825 1107 68-69 0.2504277924059295 1107 70-71 0.30350390743167566 1107 72-73 0.27677623664413176 1107 74-75 0.2892094909545335 1107 76-77 0.26842630052680505 1107 78-79 0.28544908596965257 1107 80-81 0.2929553032694656 1107 82-83 0.25524525567917067 1107 84-85 0.25620206410023627 1107 86-87 0.22884478112538176 1107 88-89 0.22774092577976646 1107 90-91 0.2982717935020247 1107 92-93 0.27590036422326847 1107 94-95 0.2837425023504494 1107 96-97 0.30768918663093103 1107 98-99 0.39336605664149005 1107 100 0.3428810763155319 1108 1 0.16575838351506889 1108 2 0.22292902369755296 1108 3 0.26157930504926696 1108 4 0.17839846197222187 1108 5 0.22138755598712123 1108 6 0.230252520870593 1108 7 0.23061066639571948 1108 8 0.23301068764695998 1108 9 0.2483279866281407 1108 10-11 0.2048869813253802 1108 12-13 0.24316194708889327 1108 14-15 0.3140466967608475 1108 16-17 0.223787342557749 1108 18-19 0.27383552204246797 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-0.692546435619505 2215 28-29 -0.6322714862759256 2215 30-31 -0.8541565866655532 2215 32-33 -0.8215513502834 2215 34-35 -0.6463770089944276 2215 36-37 -0.6144421738922148 2215 38-39 -0.6611435000751698 2215 40-41 -0.579991855855134 2215 42-43 -0.6034845138705265 2215 44-45 -0.5614791921863969 2215 46-47 -0.431724399768342 2215 48-49 -0.5019433370107009 2215 50-51 -0.5795019686566505 2215 52-53 -0.5481922471680818 2215 54-55 -0.5429284026405412 2215 56-57 -0.5309169919456593 2215 58-59 -0.533426401071381 2215 60-61 -0.47803170458337263 2215 62-63 -0.5078376134776121 2215 64-65 -0.5487452586454111 2215 66-67 -0.5260926323571553 2215 68-69 -0.5139693999326695 2215 70-71 -0.492572635187706 2215 72-73 -0.4010298039802649 2215 74-75 -0.44563326520730584 2215 76-77 -0.44742352638949967 2215 78-79 -0.4118357836269908 2215 80-81 -0.4570042198446558 2215 82-83 -0.4389179054966945 2215 84-85 -0.4515542191059403 2215 86-87 -0.4890253724694098 2215 88-89 -0.42954632176664376 2215 90-91 -0.41323105581864183 2215 92-93 -0.4214733555495904 2215 94-95 -0.4974570441306376 2215 96-97 -0.5590430530654302 2215 98-99 -0.5451863428254562 2215 100 -0.589682136502951 2216 1 -0.3590558717759649 2216 2 -0.5758354230390239 2216 3 -0.567107273078058 2216 4 -0.6192283129308578 2216 5 -0.5345685075437245 2216 6 -0.5949278912309595 2216 7 -0.5755252061997638 2216 8 -0.5655889845891338 2216 9 -0.6714490719584418 2216 10-11 -0.7220074804903192 2216 12-13 -0.7379673134469584 2216 14-15 -0.8402048662521437 2216 16-17 -0.8291120095671403 2216 18-19 -0.9179869218228021 2216 20-21 -0.8920156114531181 2216 22-23 -0.9118687397974554 2216 24-25 -0.9272641522384149 2216 26-27 -0.9180649784090491 2216 28-29 -0.9014895561707519 2216 30-31 -1.023100468869643 2216 32-33 -0.9997071992218025 2216 34-35 -0.9841114067210626 2216 36-37 -1.0176391566305867 2216 38-39 -0.8861872566136668 2216 40-41 -0.870068302613042 2216 42-43 -0.8659605789112845 2216 44-45 -0.946203592338712 2216 46-47 -0.8278398050060076 2216 48-49 -0.9647651772347174 2216 50-51 -0.9342858860579426 2216 52-53 -1.0279503013750357 2216 54-55 -0.9778880982921976 2216 56-57 -0.96640278240573 2216 58-59 -0.9355415620471064 2216 60-61 -0.9096728398739913 2216 62-63 -0.9513185442206051 2216 64-65 -0.9207366274924453 2216 66-67 -0.926170529667317 2216 68-69 -0.8102032292957801 2216 70-71 -0.8714830931443842 2216 72-73 -0.8341954504985765 2216 74-75 -0.9054231208951506 2216 76-77 -0.9208227637682853 2216 78-79 -0.9454898721403779 2216 80-81 -0.9278713231714057 2216 82-83 -0.925946823263871 2216 84-85 -0.8433363521416268 2216 86-87 -0.9337093864010342 2216 88-89 -0.9213667767104248 2216 90-91 -0.9278422219881293 2216 92-93 -0.9231730115050176 2216 94-95 -0.910752041628168 2216 96-97 -0.9138018045989824 2216 98-99 -0.9504036755303105 2216 100 -1.009290429799762 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 87852.0 3 18066.0 4 10628.0 5 8960.0 6 7573.0 7 8544.0 8 10113.0 9 12044.0 10 13534.0 11 15155.0 12 17300.0 13 19409.0 14 22768.0 15 23789.0 16 24036.0 17 25297.0 18 25000.0 19 21202.0 20 20437.0 21 21695.0 22 23892.0 23 28690.0 24 36036.0 25 45921.0 26 54945.0 27 62112.0 28 74840.0 29 95472.0 30 114078.0 31 129791.0 32 165952.0 33 221864.0 34 304374.0 35 438846.0 36 740190.0 37 1383678.0 38 2055746.0 39 923442.0 40 3109.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.63047702292065 21.880228242202648 15.147739840000657 22.34155489487604 2 15.151146065806067 20.913366835379847 41.26483485328296 22.670652245531123 3 17.8781972344223 26.508283172059006 31.060733236802047 24.552786356716652 4 12.08488932272889 17.972048304235265 40.81142782312032 29.131634549915532 5 12.150254371640944 38.73696394891298 36.396400386348546 12.716381293097534 6 28.62781334384938 39.70951932152374 18.694866798663988 12.967800535962892 7 25.23829613410969 33.42739315977584 23.905855596726003 17.428455109388466 8 23.545170882216528 36.980048124207094 21.548712056189654 17.926068937386724 9 24.139201804999423 17.537342046488906 23.403084415517846 34.92037173299383 10-11 22.58565381256299 28.028754927784604 30.071580432913713 19.314010826738688 12-13 23.2138992891946 25.90348920326831 29.742476750196133 21.14013475734096 14-15 20.82575761200232 28.30431879337059 27.230672598295286 23.639250996331803 16-17 19.737890724533393 31.448526830580597 28.116452943550197 20.697129501335816 18-19 19.55916105740874 30.35911097356888 30.2533115957075 19.82841637331488 20-21 21.277835327336696 29.44743948205944 29.493417615414202 19.78130757518966 22-23 20.724038256292886 29.478785383410806 29.669090817378347 20.12808554291797 24-25 20.36874766324478 29.6691052081532 29.69596037820063 20.266186750401392 26-27 20.0180850945319 29.683345454078232 29.80495613055795 20.493613320831912 28-29 20.002476933725553 29.65226625424535 30.136587448302382 20.208669363726713 30-31 20.568770172290662 30.214999548565807 29.349107079574825 19.867123199568702 32-33 19.968071513685288 29.881308104219983 29.889345930119305 20.261274451975424 34-35 20.39589778232477 29.44792102302151 29.871052010528317 20.285129184125406 36-37 20.534949837816217 29.86857369104917 29.345494343234453 20.250982127900155 38-39 20.304316175490523 29.983964292183447 29.578505589599345 20.133213942726684 40-41 20.33639307810862 29.471658339109414 29.743428579591423 20.44852000319054 42-43 20.12630958800561 29.598154940945943 29.711540367330507 20.563995103717936 44-45 20.43741780430521 29.72529207667679 29.479258659769293 20.358031459248707 46-47 20.409138759248066 29.851631082017978 29.374917250235494 20.364312908498462 48-49 20.129314920327182 29.75269823604491 29.4183241432121 20.69966270041581 50-51 20.26724111004649 29.614022957914543 29.29673539578792 20.822000536251043 52-53 20.304129614557826 29.620497189703425 29.305553694995268 20.76981950074348 54-55 20.463755385331886 29.99109558602704 29.177265776252607 20.367883252388467 56-57 20.367305694654565 29.708352213348398 29.104066415182245 20.820275676814788 58-59 20.364563621082475 29.348771131020357 29.457365771488718 20.829299476408448 60-61 20.469830284350245 29.73681338249688 29.103162180166375 20.690194152986503 62-63 20.26057204349347 29.739216069306522 29.24107609716901 20.759135790030996 64-65 20.4473137407794 29.46148081548091 29.2066661011257 20.88453934261399 66-67 20.155931855851545 29.54261401500265 29.128260683833705 21.1731934453121 68-69 20.675109205525104 29.842946358574146 29.07532601036043 20.40661842554032 70-71 20.666433886518856 29.545549624553146 28.980287604703214 20.80772888422478 72-73 20.42551103047126 29.58774970511952 29.245820954368305 20.74091831004092 74-75 20.507831697955865 29.632028107302638 29.042752218952607 20.817387975788897 76-77 20.52774300839172 29.632427026778025 28.75446832365023 21.08536164118003 78-79 20.649120557541586 29.58622079232932 29.115216858787264 20.649441791341832 80-81 20.502864506328407 29.563746356225185 29.07246646660851 20.860922670837894 82-83 20.411938621007405 29.608808608702603 29.348810381666325 20.63044238862367 84-85 20.34582395319117 29.37839337421706 29.156215745023985 21.11956692756778 86-87 20.65354814927772 29.64197972954823 28.927303484196216 20.777168636977837 88-89 20.496428097123825 29.432675182566314 29.06777152316093 21.003125197148933 90-91 20.341466742162666 29.303695685873947 29.08152064105617 21.273316930907217 92-93 20.57669673443665 29.55064151645938 28.85307134885596 21.019590400248013 94-95 20.451919700793503 29.216186454110616 29.145224771573712 21.186669073522168 96-97 20.475105769185422 29.48738059252069 28.913046345969317 21.12446729232457 98-99 20.54265897113684 29.386704201124473 28.85060929475398 21.220027532984698 100 20.644077999710763 29.331208441102042 28.714203270664594 21.310510288522604 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4113.0 1 2792.5 2 1669.5 3 1903.0 4 1824.5 5 1546.5 6 1387.0 7 1384.5 8 1457.5 9 1542.0 10 1399.0 11 1243.5 12 1272.5 13 1400.5 14 1594.5 15 1910.0 16 2371.5 17 3027.5 18 4114.5 19 5820.5 20 8489.0 21 12466.5 22 18825.5 23 29124.5 24 40941.0 25 53675.0 26 71564.5 27 94219.5 28 122482.5 29 149838.0 30 171163.5 31 193008.5 32 216884.5 33 237573.5 34 256277.0 35 271982.0 36 278721.5 37 283836.0 38 289219.0 39 287262.5 40 286232.5 41 282843.0 42 270761.5 43 262168.5 44 251680.0 45 241670.0 46 236937.0 47 230172.0 48 225809.5 49 221687.5 50 212548.5 51 195655.5 52 174973.5 53 156345.0 54 145413.5 55 141662.0 56 125760.5 57 105843.0 58 91006.0 59 76930.0 60 62139.5 61 46437.5 62 35220.5 63 27043.0 64 20958.0 65 15986.5 66 11859.5 67 8553.0 68 6052.0 69 4395.5 70 3213.5 71 2335.0 72 1709.5 73 1266.0 74 981.5 75 725.5 76 531.0 77 380.0 78 276.5 79 203.5 80 132.5 81 107.0 82 94.5 83 64.0 84 53.0 85 48.5 86 33.0 87 26.0 88 18.0 89 14.5 90 16.5 91 17.0 92 11.5 93 10.0 94 14.0 95 10.0 96 9.0 97 9.5 98 8.5 99 11.0 100 20.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07824907946279444 2 0.013749969247086675 3 0.01787769361350832 4 0.011358075988398634 5 0.009649580803621462 6 0.007859077849974987 7 0.02093931698462901 8 0.009936607994664028 9 0.009553905073273942 10-11 0.013298926518305502 12-13 0.015813831430297496 14-15 0.010866029375182808 16-17 0.0136132896323045 18-19 0.008843171076406638 20-21 0.012929891558393632 22-23 0.014139506149215868 24-25 0.016606573196034105 26-27 0.00561069818680823 28-29 0.012075643966005047 30-31 0.011023210932182309 32-33 0.013244254672392632 34-35 0.009273711862970485 36-37 0.010380816742706092 38-39 0.01988688395080627 40-41 0.013797807112260436 42-43 0.008542475923885856 44-45 0.020775301446890403 46-47 0.019196651896156297 48-49 0.018718273244418687 50-51 0.010613172087835788 52-53 0.010688345875965984 54-55 0.012396841060743154 56-57 0.021137502426063163 58-59 0.002473901027557344 60-61 0.007182513756803228 62-63 0.01595051104507967 64-65 0.025278894753963024 66-67 0.013736301285608456 68-69 0.014283019744737151 70-71 0.025914454962700135 72-73 0.021212676214193357 74-75 0.021253680098628012 76-77 0.009799928379881855 78-79 0.011426415795789721 80-81 0.020597617947673577 82-83 0.012923057577654523 84-85 0.01144008375726794 86-87 0.017078117867032606 88-89 0.015875337256949475 90-91 0.01642888969681728 92-93 0.008590313789059617 94-95 0.015916341141384127 96-97 0.014221513918085175 98-99 0.01081135752926994 100 0.007626722504845293 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7316380.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.18804638794895 #Duplication Level Percentage of deduplicated Percentage of total 1 71.75077109494748 40.31535654654015 2 16.306601764750265 18.32472192775196 3 5.862548739985938 9.882154816618247 4 2.575888376463227 5.789365423475773 5 1.2796185518835097 3.5949633276055346 6 0.6937660670254141 2.3388815973845323 7 0.4161691476750667 1.6368611962359878 8 0.24969568339624337 1.1223930113230989 9 0.17743697551310444 0.8972853309960906 >10 0.5897979493505062 5.63010856971414 >50 0.04647983745300057 1.8467040536269437 >100 0.04449111687245944 5.118223896351473 >500 0.005026762246908849 1.9014139705665474 >1k 0.001683533402647394 1.530798714995715 >5k 2.4399034322488707E-5 0.07076761681383742 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.366796147821737E-5 0.0 0.0 0.0 3 0.0 1.366796147821737E-5 0.0 1.366796147821737E-5 0.0 4 0.0 1.366796147821737E-5 0.0 5.467184591286948E-5 0.0 5 0.0 1.366796147821737E-5 0.0 6.833980739108685E-5 0.0 6 0.0 1.366796147821737E-5 1.366796147821737E-5 8.200776886930422E-5 0.0 7 0.0 1.366796147821737E-5 1.366796147821737E-5 8.200776886930422E-5 0.0 8 0.0 1.366796147821737E-5 1.366796147821737E-5 9.567573034752159E-5 0.0 9 0.0 1.366796147821737E-5 1.366796147821737E-5 1.5034757626039108E-4 0.0 10-11 0.0 1.366796147821737E-5 1.366796147821737E-5 2.0501942217326054E-4 0.0 12-13 0.0 2.733592295643474E-5 1.366796147821737E-5 2.5285728734702133E-4 0.0 14-15 1.366796147821737E-5 6.833980739108685E-5 1.366796147821737E-5 3.485330176945429E-4 0.0 16-17 2.733592295643474E-5 8.200776886930422E-5 1.366796147821737E-5 4.100388443465211E-4 0.0 18-19 2.733592295643474E-5 1.0934369182573896E-4 1.366796147821737E-5 4.373747673029558E-4 0.0 20-21 2.733592295643474E-5 1.4351359552128239E-4 1.366796147821737E-5 5.330504976504774E-4 0.0 22-23 2.733592295643474E-5 1.6401553773860844E-4 1.366796147821737E-5 6.765640931717597E-4 0.0 24-25 2.733592295643474E-5 1.6401553773860844E-4 1.366796147821737E-5 9.909272071707592E-4 0.0 26-27 2.733592295643474E-5 1.9135146069504318E-4 1.366796147821737E-5 0.0017016612040380626 0.0 28-29 2.733592295643474E-5 1.9135146069504318E-4 1.366796147821737E-5 0.0034033224080761253 0.0 30-31 3.416990369554342E-5 1.9135146069504318E-4 1.366796147821737E-5 0.009991279840576898 0.0 32-33 4.100388443465211E-5 2.3918932586880398E-4 1.366796147821737E-5 0.02499186756292046 0.0 34-35 4.100388443465211E-5 2.4602330660791267E-4 1.366796147821737E-5 0.05145304098474929 0.0 36-37 4.100388443465211E-5 2.938611717816735E-4 1.366796147821737E-5 0.09280545843709595 0.0 38-39 4.100388443465211E-5 3.553669984336516E-4 1.366796147821737E-5 0.14751147425366096 0.0 40-41 4.100388443465211E-5 3.69034959911869E-4 1.366796147821737E-5 0.2008165240187087 0.0 42-43 4.100388443465211E-5 4.032048636074124E-4 1.366796147821737E-5 0.25782559134435334 0.0 44-45 5.467184591286948E-5 4.168728250856298E-4 1.366796147821737E-5 0.3323706532465509 0.0 46-47 5.467184591286948E-5 4.2370680582473846E-4 1.366796147821737E-5 0.42544947091321117 0.0 48-49 5.467184591286948E-5 4.5104272878117324E-4 1.366796147821737E-5 0.5241526547281579 0.0 50-51 5.467184591286948E-5 5.603864206069121E-4 1.366796147821737E-5 0.6228626725238438 0.0 52-53 5.467184591286948E-5 6.08224285780673E-4 1.366796147821737E-5 0.7226934631607435 0.0 54-55 5.467184591286948E-5 6.355602087371078E-4 1.366796147821737E-5 0.8366910958698154 0.0 56-57 5.467184591286948E-5 6.697301124326511E-4 1.366796147821737E-5 0.9770200563666731 0.0 58-59 5.467184591286948E-5 6.697301124326511E-4 1.366796147821737E-5 1.1320967472985275 0.0 60-61 5.467184591286948E-5 6.697301124326511E-4 1.366796147821737E-5 1.2926406228216685 0.0 62-63 5.467184591286948E-5 6.697301124326511E-4 1.366796147821737E-5 1.4556857352953236 0.0 64-65 6.150582665197817E-5 7.039000161281945E-4 1.366796147821737E-5 1.633273558781802 0.0 66-67 6.833980739108685E-5 7.17567977606412E-4 1.366796147821737E-5 1.8428444121273089 0.0 68-69 6.833980739108685E-5 7.38069919823738E-4 1.366796147821737E-5 2.0784390641273416 0.0 70-71 6.833980739108685E-5 7.38069919823738E-4 1.366796147821737E-5 2.330845308745582 0.0 72-73 6.833980739108685E-5 7.38069919823738E-4 1.366796147821737E-5 2.5932223312621816 0.0 74-75 6.833980739108685E-5 7.38069919823738E-4 1.366796147821737E-5 2.862870709285193 0.0 76-77 7.517378813019554E-5 7.38069919823738E-4 1.366796147821737E-5 3.154333153827439 0.0 78-79 8.200776886930422E-5 7.38069919823738E-4 1.366796147821737E-5 3.4837856972983907 0.0 80-81 9.567573034752159E-5 7.449039005628466E-4 1.366796147821737E-5 3.8444080269204166 0.0 82-83 9.567573034752159E-5 7.517378813019553E-4 1.366796147821737E-5 4.216456772338233 0.0 84-85 1.0250971108663028E-4 7.517378813019553E-4 1.366796147821737E-5 4.591478299377561 0.0 86-87 1.0934369182573896E-4 7.517378813019553E-4 1.366796147821737E-5 4.989297986162556 0.0 88 1.0934369182573896E-4 7.517378813019553E-4 1.366796147821737E-5 5.313392688734046 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8190 0.0 52.881573 1 GTATCAA 15195 0.0 37.10625 1 ATCAACG 16195 0.0 33.694355 3 TCAACGC 16580 0.0 32.88292 4 TATCAAC 18660 0.0 29.8728 2 CAACGCA 19145 0.0 28.551779 5 AACGCAG 20900 0.0 26.24474 6 CGCAGAG 20370 0.0 26.094423 8 ACGCAGA 21525 0.0 25.024826 7 GTGGTAT 4450 0.0 23.776676 1 GTACATG 23985 0.0 23.566406 1 TGGTATC 4030 0.0 23.325333 2 TACATGG 23950 0.0 22.587736 2 GCAGAGT 22955 0.0 22.255802 9 ACATGGG 24755 0.0 21.188837 3 GCGGGTA 485 0.0 20.350195 9 CAGAGTA 23325 0.0 18.981052 10-11 TCTGTCG 1880 0.0 18.249088 8 CATGGGA 10710 0.0 18.124144 4 AGAGTAC 21840 0.0 17.80764 10-11 >>END_MODULE