FastQCFastQC Report
Wed 25 May 2016
SRR2049456_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049456_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7316380
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258160.35285209352165964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257810.352373714869922No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135460.1851462061839325No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86630.11840555028579708No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA155750.063.469551
GTATCAA295000.040.9138341
TGGTATC54750.037.862632
GTGGTAT56100.037.675381
ATCAACG367350.032.322853
TCAACGC373100.031.8247054
TATCAAC378900.031.486422
CAACGCA394500.030.1340945
AACGCAG415500.028.6403146
CGCAGAG448700.026.1964668
ACGCAGA454550.025.9730577
GCAGAGT481050.024.0439859
GTACATG227200.022.749311
GAGTACT367000.021.76407412-13
TACATGG230900.021.721852
CAGAGTA486750.021.52232610-11
ACATGGG232600.020.5930563
AGAGTAC468800.020.58695810-11
GTACTTT395650.020.11680814-15
AGTACTT384900.019.18896512-13
Produced by FastQC (version 0.11.5)