##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049456_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7316380 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.927855168812993 33.0 31.0 34.0 30.0 34.0 2 32.15731071923547 33.0 31.0 34.0 30.0 34.0 3 32.159533676490284 34.0 31.0 34.0 30.0 34.0 4 35.84453841927292 37.0 35.0 37.0 35.0 37.0 5 35.900058498875126 37.0 35.0 37.0 35.0 37.0 6 35.96568056880589 37.0 35.0 37.0 35.0 37.0 7 35.93789920698487 37.0 35.0 37.0 35.0 37.0 8 35.9496662283807 37.0 35.0 37.0 35.0 37.0 9 37.67347322036308 39.0 37.0 39.0 35.0 39.0 10-11 37.64729586216134 39.0 37.0 39.0 35.0 39.0 12-13 37.622243855567916 39.0 37.0 39.0 35.0 39.0 14-15 38.90586868095971 40.0 38.0 41.0 35.0 41.0 16-17 38.92189040208409 40.0 38.0 41.0 35.5 41.0 18-19 39.01902101859116 40.0 38.5 41.0 36.0 41.0 20-21 39.05280616917109 40.0 39.0 41.0 35.5 41.0 22-23 39.01830789270103 40.0 39.0 41.0 35.0 41.0 24-25 38.99120029304109 40.0 39.0 41.0 35.0 41.0 26-27 38.88540869938412 40.0 38.0 41.0 35.0 41.0 28-29 38.80292904414478 40.0 38.0 41.0 35.0 41.0 30-31 38.66868444503976 40.0 38.0 41.0 35.0 41.0 32-33 38.501997982608884 40.0 38.0 41.0 34.5 41.0 34-35 38.37406811838642 40.0 38.0 41.0 34.0 41.0 36-37 38.21911402906902 40.0 38.0 41.0 34.0 41.0 38-39 38.09624479592367 40.0 38.0 41.0 34.0 41.0 40-41 37.98673640516212 40.0 38.0 41.0 33.5 41.0 42-43 37.82017233932628 40.0 38.0 41.0 33.0 41.0 44-45 37.602547571339926 40.0 38.0 41.0 33.0 41.0 46-47 37.29190357799896 40.0 37.0 41.0 32.5 41.0 48-49 36.987501332626245 40.0 37.0 41.0 31.0 41.0 50-51 36.8050456646593 40.0 37.0 41.0 31.0 41.0 52-53 36.67794264376646 40.0 37.0 41.0 31.0 41.0 54-55 36.536121073536364 40.0 36.5 41.0 31.0 41.0 56-57 36.39683791711201 40.0 36.0 41.0 30.0 41.0 58-59 36.2276647741096 39.5 36.0 41.0 30.0 41.0 60-61 36.05453291108444 39.0 35.0 41.0 30.0 41.0 62-63 35.85275423091747 39.0 35.0 41.0 29.0 41.0 64-65 35.64997567102857 39.0 35.0 41.0 29.0 41.0 66-67 35.405149062787885 38.0 35.0 40.5 29.0 41.0 68-69 35.12657304568653 37.5 35.0 40.0 29.0 41.0 70-71 34.8521284433012 37.0 35.0 40.0 28.0 41.0 72-73 34.52558984087759 37.0 34.5 39.5 28.0 41.0 74-75 34.203182311470975 36.0 34.0 39.0 28.0 41.0 76-77 33.883003343183375 36.0 34.0 39.0 28.0 40.5 78-79 33.56266152113477 35.0 34.0 37.5 27.5 39.5 80-81 33.238579666447066 35.0 34.0 37.0 27.0 39.0 82-83 32.951327432418765 35.0 34.0 37.0 27.0 39.0 84-85 32.64921887600152 35.0 34.0 36.0 26.0 38.5 86-87 32.383294265743444 35.0 34.0 36.0 26.0 37.0 88-89 32.14776392150216 35.0 33.5 36.0 26.0 37.0 90-91 31.937417206323346 35.0 33.0 35.0 26.0 36.5 92-93 31.759230384425088 35.0 33.0 35.0 25.0 36.0 94-95 31.587120953258307 35.0 33.0 35.0 25.0 36.0 96-97 31.43566941574932 35.0 33.0 35.0 25.0 36.0 98-99 31.29576170182522 35.0 33.0 35.0 24.5 36.0 100 31.181087505022976 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3462101308839287 1101 2 0.39838876586401994 1101 3 0.500641335188881 1101 4 0.3117589460179815 1101 5 0.2877106137438119 1101 6 0.23796020851956712 1101 7 0.2561869243939725 1101 8 0.27906200979911233 1101 9 0.3342335594464245 1101 10-11 0.33582307220403607 1101 12-13 0.33774781498004103 1101 14-15 0.46089155569007545 1101 16-17 0.4497817408995104 1101 18-19 0.4329369562972758 1101 20-21 0.4456271205811859 1101 22-23 0.45454060397278084 1101 24-25 0.41606632830635704 1101 26-27 0.4267562547901349 1101 28-29 0.4409274326708754 1101 30-31 0.4664829968819504 1101 32-33 0.45862760482837217 1101 34-35 0.35357919148518135 1101 36-37 0.37653998045042414 1101 38-39 0.4040998470588306 1101 40-41 0.39980429745006063 1101 42-43 0.4146944576074105 1101 44-45 0.40567522752237295 1101 46-47 0.35890423222206636 1101 48-49 0.3518236336099534 1101 50-51 0.3075716215290569 1101 52-53 0.2959902551780189 1101 54-55 0.28061331063441486 1101 56-57 0.35748225200025985 1101 58-59 0.3356360229869395 1101 60-61 0.2246914477428632 1101 62-63 0.28992273529248536 1101 64-65 0.29848426704391784 1101 66-67 0.3332396364996413 1101 68-69 0.3482841405068413 1101 70-71 0.313837583359863 1101 72-73 0.3216854941742042 1101 74-75 0.2900877511772819 1101 76-77 0.29091171243824476 1101 78-79 0.2953704477691801 1101 80-81 0.2560347857681151 1101 82-83 0.20428627711433478 1101 84-85 0.22929497337831606 1101 86-87 0.21235803419111932 1101 88-89 0.2496026944043308 1101 90-91 0.2571824301748009 1101 92-93 0.2583989861038596 1101 94-95 0.2610536850160763 1101 96-97 0.28952528568070335 1101 98-99 0.34336009147057567 1101 100 0.3489838692565215 1102 1 0.2607656136900296 1102 2 0.29037502198265486 1102 3 0.3642198539040038 1102 4 0.22271978818265126 1102 5 0.21262244844663059 1102 6 0.20918311474877527 1102 7 0.21541484457082305 1102 8 0.19641758658897146 1102 9 0.25522566025251336 1102 10-11 0.25857860058475524 1102 12-13 0.26872447869530447 1102 14-15 0.33081368169864334 1102 16-17 0.35542391800790796 1102 18-19 0.37042899144906016 1102 20-21 0.37372695700862835 1102 22-23 0.3131538221649066 1102 24-25 0.3158665149940916 1102 26-27 0.2992237106939939 1102 28-29 0.3774931947782818 1102 30-31 0.34356996162815534 1102 32-33 0.38513314424842093 1102 34-35 0.4089926732041178 1102 36-37 0.40050338939963837 1102 38-39 0.3589999242733697 1102 40-41 0.3764356535760953 1102 42-43 0.3627856677519006 1102 44-45 0.34988699536514645 1102 46-47 0.33497160120735003 1102 48-49 0.3770176681270385 1102 50-51 0.3496742653060281 1102 52-53 0.37717151547732186 1102 54-55 0.3325227139332867 1102 56-57 0.36255392677627896 1102 58-59 0.32772986966872253 1102 60-61 0.29586872647740137 1102 62-63 0.3039617040709146 1102 64-65 0.24091377565832772 1102 66-67 0.25165007146748053 1102 68-69 0.2521407584846145 1102 70-71 0.25353900355001 1102 72-73 0.2588244662260877 1102 74-75 0.2594275766452441 1102 76-77 0.2847093874984594 1102 78-79 0.2629483845592375 1102 80-81 0.2656418218789369 1102 82-83 0.28941338495994984 1102 84-85 0.21544673260433456 1102 86-87 0.20881338918419345 1102 88-89 0.23894249766140518 1102 90-91 0.23326656308661597 1102 92-93 0.23284697036332958 1102 94-95 0.19686165947313938 1102 96-97 0.22923633587406655 1102 98-99 0.23568431786693012 1102 100 0.2609090126660938 1103 1 0.3057622081500959 1103 2 0.32742113938849116 1103 3 0.38780138424108657 1103 4 0.28448830912522993 1103 5 0.2517963844371778 1103 6 0.24329517522198074 1103 7 0.20562813821668158 1103 8 0.20593767550924724 1103 9 0.27428382144048413 1103 10-11 0.2785267868557213 1103 12-13 0.28183066397577505 1103 14-15 0.3640289394453404 1103 16-17 0.3180558474743833 1103 18-19 0.32990317162455085 1103 20-21 0.318100336605859 1103 22-23 0.34951490055004086 1103 24-25 0.36786872275874316 1103 26-27 0.3428374851428799 1103 28-29 0.3468135438335409 1103 30-31 0.37812213214739643 1103 32-33 0.4079410442335458 1103 34-35 0.4017402783689761 1103 36-37 0.38706883482230126 1103 38-39 0.41589450622214486 1103 40-41 0.4422944070638266 1103 42-43 0.46430646853560376 1103 44-45 0.4898965876886834 1103 46-47 0.4727055771335884 1103 48-49 0.45192855190494186 1103 50-51 0.48293940952118675 1103 52-53 0.5066999490322743 1103 54-55 0.48797635682479523 1103 56-57 0.4326077855462742 1103 58-59 0.4112101347824293 1103 60-61 0.3749978348326124 1103 62-63 0.4315207351618966 1103 64-65 0.37987726129976807 1103 66-67 0.38790759047222423 1103 68-69 0.40297659706089917 1103 70-71 0.3964554970478886 1103 72-73 0.4632428029869544 1103 74-75 0.4626163434451769 1103 76-77 0.438238013960202 1103 78-79 0.4850348631333432 1103 80-81 0.4223143863003642 1103 82-83 0.41731777464083564 1103 84-85 0.43038862605924066 1103 86-87 0.4105041009720125 1103 88-89 0.44672137080678453 1103 90-91 0.38943500638450246 1103 92-93 0.4164035095708378 1103 94-95 0.3883723657852265 1103 96-97 0.4531538776775932 1103 98-99 0.4801240521001624 1103 100 0.463618961544892 1104 1 0.2617781597363411 1104 2 0.2607650732339337 1104 3 0.3165657703834768 1104 4 0.17814510984238296 1104 5 0.1571509422492383 1104 6 0.13294551123637177 1104 7 0.16213246643336987 1104 8 0.14975984766454786 1104 9 0.19822669916679558 1104 10-11 0.2157336128497036 1104 12-13 0.200256733969276 1104 14-15 0.3278465718910937 1104 16-17 0.28887114010428405 1104 18-19 0.2664945183184386 1104 20-21 0.27609517500725644 1104 22-23 0.24308657812017032 1104 24-25 0.27129046919196753 1104 26-27 0.31379635032701003 1104 28-29 0.29105453394436864 1104 30-31 0.3042289313871578 1104 32-33 0.3352519558741349 1104 34-35 0.311109679542362 1104 36-37 0.3347923288568708 1104 38-39 0.350035260557803 1104 40-41 0.3146240294131104 1104 42-43 0.3880720291273576 1104 44-45 0.4338383174094318 1104 46-47 0.4122096071102703 1104 48-49 0.36790111209992915 1104 50-51 0.40992323730172586 1104 52-53 0.4462561192719008 1104 54-55 0.43519108089013514 1104 56-57 0.44342663141723904 1104 58-59 0.42462698279027933 1104 60-61 0.3543565711090011 1104 62-63 0.3863254029053991 1104 64-65 0.34241415182924584 1104 66-67 0.3806874431297018 1104 68-69 0.35139451374294595 1104 70-71 0.3405353955910968 1104 72-73 0.3359655755658437 1104 74-75 0.32595260399151016 1104 76-77 0.313911683978219 1104 78-79 0.3262054902180225 1104 80-81 0.326764393905826 1104 82-83 0.30808182552191 1104 84-85 0.27513064558096545 1104 86-87 0.2627004678706797 1104 88-89 0.31445803672497163 1104 90-91 0.3078698861853866 1104 92-93 0.33488629000467895 1104 94-95 0.29383903665990374 1104 96-97 0.29179376183997974 1104 98-99 0.3657416377059093 1104 100 0.3293784221282898 1105 1 0.13272452756187292 1105 2 0.13276404221368665 1105 3 0.23070111544422645 1105 4 0.14246532121037347 1105 5 0.16119323132198105 1105 6 0.08366925362765443 1105 7 0.12763230511539803 1105 8 0.10072752891571213 1105 9 0.1531881469897982 1105 10-11 0.09266687252609529 1105 12-13 0.09806895498562085 1105 14-15 0.20028181507669984 1105 16-17 0.2007688821942537 1105 18-19 0.1556209906143593 1105 20-21 0.1733034622082883 1105 22-23 0.128032143390179 1105 24-25 0.18545123606412517 1105 26-27 0.18249494645721853 1105 28-29 0.20200096145590152 1105 30-31 0.19208800203867327 1105 32-33 0.15153254535952243 1105 34-35 0.15469801961855723 1105 36-37 0.18258592010663932 1105 38-39 0.14176502581833006 1105 40-41 0.1403391919430419 1105 42-43 0.08845091912044012 1105 44-45 0.11139376763026831 1105 46-47 0.12399936729305949 1105 48-49 0.1425313242206272 1105 50-51 0.1279002657108137 1105 52-53 0.1299868867044367 1105 54-55 0.14226418318783374 1105 56-57 0.12250179476067302 1105 58-59 0.14455741489807394 1105 60-61 0.09137692646392992 1105 62-63 0.12724073898893096 1105 64-65 0.11517494894229685 1105 66-67 0.09234956923979354 1105 68-69 0.09256025466882534 1105 70-71 0.08556815325304257 1105 72-73 0.12780031632802036 1105 74-75 0.1283469572987599 1105 76-77 0.1291323359062062 1105 78-79 0.10539297651925494 1105 80-81 0.12782307971534124 1105 82-83 0.0849968920237103 1105 84-85 0.06926954195823498 1105 86-87 0.052638948745020286 1105 88-89 0.058643584889409794 1105 90-91 0.05851832198685969 1105 92-93 0.07307061891694033 1105 94-95 0.11528008806623546 1105 96-97 0.13866821644541005 1105 98-99 0.14334805313213295 1105 100 0.11772305214090295 1106 1 0.10940229628884524 1106 2 0.1187360547983971 1106 3 0.22978262316909337 1106 4 0.13275020821954087 1106 5 0.10462747654721483 1106 6 0.11686397918069247 1106 7 0.10211479852194572 1106 8 0.08947066950373284 1106 9 0.1093091211096251 1106 10-11 0.1357303508317642 1106 12-13 0.11398306743406295 1106 14-15 0.19503890760955045 1106 16-17 0.16753355316447482 1106 18-19 0.1464538269437483 1106 20-21 0.1275486663672396 1106 22-23 0.14799657780100972 1106 24-25 0.12168280393667885 1106 26-27 0.10482849229349256 1106 28-29 0.14185245707218996 1106 30-31 0.17159754049624354 1106 32-33 0.20448597401485102 1106 34-35 0.13394641527401774 1106 36-37 0.14247262296863283 1106 38-39 0.14846370362315042 1106 40-41 0.16420451118557366 1106 42-43 0.18711709095693152 1106 44-45 0.24784396454131752 1106 46-47 0.2260300549317762 1106 48-49 0.19100175752507198 1106 50-51 0.17690336604053414 1106 52-53 0.15879880815923997 1106 54-55 0.1670452676063121 1106 56-57 0.17384475936515997 1106 58-59 0.1479978942456981 1106 60-61 0.14971529570868114 1106 62-63 0.15437505969025977 1106 64-65 0.14873241821006644 1106 66-67 0.1723851611777505 1106 68-69 0.13117899605853722 1106 70-71 0.1912044744199548 1106 72-73 0.18392208091138684 1106 74-75 0.20125049980060083 1106 76-77 0.17220234871039963 1106 78-79 0.15914485238802456 1106 80-81 0.10319450206092284 1106 82-83 0.12725938045824137 1106 84-85 0.13022442453949168 1106 86-87 0.11715394732943452 1106 88-89 0.08604022091375896 1106 90-91 0.10157650418685904 1106 92-93 0.14140429412574917 1106 94-95 0.10895688043899909 1106 96-97 0.1524139293335125 1106 98-99 0.1923530637487154 1106 100 0.16800406851364258 1107 1 0.13770701744846647 1107 2 0.12812314152944282 1107 3 0.20835290588905053 1107 4 0.12211382788827763 1107 5 0.148388873267713 1107 6 0.08158319722003426 1107 7 0.10625534533988201 1107 8 0.10840925681732472 1107 9 0.06858992114330675 1107 10-11 0.0631661817768574 1107 12-13 0.12623413738456435 1107 14-15 0.20099374540141213 1107 16-17 0.19048571163044414 1107 18-19 0.16804972737370605 1107 20-21 0.15695309729013474 1107 22-23 0.1796148824474244 1107 24-25 0.16635443080405565 1107 26-27 0.15306475722765356 1107 28-29 0.164788738301624 1107 30-31 0.21969228689179943 1107 32-33 0.19103792933663755 1107 34-35 0.17492517005360497 1107 36-37 0.17277075228813032 1107 38-39 0.18623648542013882 1107 40-41 0.18081299580342147 1107 42-43 0.14760027918034524 1107 44-45 0.1526330584675435 1107 46-47 0.17976979653735015 1107 48-49 0.14692728788071463 1107 50-51 0.15091962661097824 1107 52-53 0.1323090367099482 1107 54-55 0.16198341035727282 1107 56-57 0.13553332965083342 1107 58-59 0.13414581190178865 1107 60-61 0.08742826761783107 1107 62-63 0.07139234092210245 1107 64-65 0.08311737777145822 1107 66-67 0.12259694282199973 1107 68-69 0.13429516131067487 1107 70-71 0.1245030974117256 1107 72-73 0.0866077619200567 1107 74-75 0.12777277735808923 1107 76-77 0.11706274578572362 1107 78-79 0.13103375328336853 1107 80-81 0.14260836093754392 1107 82-83 0.1635555581444521 1107 84-85 0.12529361360921598 1107 86-87 0.12774851901205153 1107 88-89 0.15958209414813496 1107 90-91 0.14940132067070522 1107 92-93 0.16128807951060864 1107 94-95 0.11512004362029415 1107 96-97 0.12290065488699042 1107 98-99 0.12299022274019578 1107 100 0.16197598852294348 1108 1 0.07694463742707214 1108 2 0.13383603221734575 1108 3 0.19780190179749013 1108 4 0.12365124387826398 1108 5 0.11194464699429574 1108 6 0.11444203947146292 1108 7 0.11047712868745663 1108 8 0.10087432917060823 1108 9 0.1635776013933068 1108 10-11 0.09264678177974872 1108 12-13 0.085112454425186 1108 14-15 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0.4151924147005701 2203 4 0.22589379613405924 2203 5 0.1790149812504609 2203 6 0.19615908467304166 2203 7 0.22019720947172772 2203 8 0.22479229125966782 2203 9 0.28242925998858937 2203 10-11 0.28620105243275873 2203 12-13 0.27905761432104015 2203 14-15 0.38888136556882813 2203 16-17 0.07066193344395799 2203 18-19 0.16531594483540601 2203 20-21 0.2761518518738768 2203 22-23 0.3682943807322374 2203 24-25 0.3936746725293432 2203 26-27 0.36980375326443493 2203 28-29 0.3789225255114914 2203 30-31 0.37659184178930616 2203 32-33 0.360860541399731 2203 34-35 0.4057981863483775 2203 36-37 0.47613191892423146 2203 38-39 0.5002734014088119 2203 40-41 0.42021497329800184 2203 42-43 0.40272035096058545 2203 44-45 0.4231290189578303 2203 46-47 0.43960825428416683 2203 48-49 0.41646492180770167 2203 50-51 0.5056955313272127 2203 52-53 0.4674545207995564 2203 54-55 0.43597331400617634 2203 56-57 0.258553892564116 2203 58-59 0.3302286268604675 2203 60-61 0.28024042865529 2203 62-63 0.4177402101674801 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-0.21910523753317435 2214 96-97 -0.1903813687197129 2214 98-99 -0.22667406767982 2214 100 -0.21476333826635496 2215 1 -0.3460323585626526 2215 2 -0.3895268051663514 2215 3 -0.3003866949647218 2215 4 -0.3012802321751593 2215 5 -0.2846641770622824 2215 6 -0.20089138592429379 2215 7 -0.2521818013468007 2215 8 -0.32349027952761134 2215 9 -0.3865560465320641 2215 10-11 -0.42689137690004486 2215 12-13 -0.4156353839422664 2215 14-15 -0.5867156260784796 2215 16-17 -0.5874665166505153 2215 18-19 -0.5875270006236661 2215 20-21 -0.5287173524163578 2215 22-23 -0.5354747505979134 2215 24-25 -0.5263443570978623 2215 26-27 -0.4901389960160287 2215 28-29 -0.47550328351370297 2215 30-31 -0.5168767121785152 2215 32-33 -0.5093312779223425 2215 34-35 -0.5189082311608004 2215 36-37 -0.5402611763753171 2215 38-39 -0.5469644575508426 2215 40-41 -0.5966961622374214 2215 42-43 -0.555797226965673 2215 44-45 -0.5735889005720693 2215 46-47 -0.5606861503285288 2215 48-49 -0.46322083845772966 2215 50-51 -0.4843874582729697 2215 52-53 -0.5595549685929342 2215 54-55 -0.5292832948006065 2215 56-57 -0.4606692602795377 2215 58-59 -0.4517187629561761 2215 60-61 -0.4498385423392861 2215 62-63 -0.4912875119297553 2215 64-65 -0.5046254440444358 2215 66-67 -0.4436042029066982 2215 68-69 -0.35605021679427296 2215 70-71 -0.302763475375464 2215 72-73 -0.304672235865894 2215 74-75 -0.331755604503698 2215 76-77 -0.3555273863408459 2215 78-79 -0.3899246375613572 2215 80-81 -0.399933386092286 2215 82-83 -0.41782236582137244 2215 84-85 -0.3555730685192202 2215 86-87 -0.3015063260519142 2215 88-89 -0.32208150672966696 2215 90-91 -0.33550271249252717 2215 92-93 -0.29303706490869175 2215 94-95 -0.2881801395144805 2215 96-97 -0.3270021557919911 2215 98-99 -0.33082284297563547 2215 100 -0.32905672054417323 2216 1 -0.36101894053076933 2216 2 -0.3634947222704241 2216 3 -0.2877787250919397 2216 4 -0.22350398017182727 2216 5 -0.294340648009225 2216 6 -0.24674321864541326 2216 7 -0.35113876449452874 2216 8 -0.2803591046582028 2216 9 -0.4221025439311177 2216 10-11 -0.4051055415400384 2216 12-13 -0.4394964180996439 2216 14-15 -0.622687597162745 2216 16-17 -0.6303378823084813 2216 18-19 -0.5596438769663692 2216 20-21 -0.5446572165511583 2216 22-23 -0.5224076857342226 2216 24-25 -0.5520889296948184 2216 26-27 -0.5680344908683423 2216 28-29 -0.6109948869109232 2216 30-31 -0.5702669898985704 2216 32-33 -0.5687838392735287 2216 34-35 -0.5503253580442049 2216 36-37 -0.6812206023563903 2216 38-39 -0.5810897609867283 2216 40-41 -0.6468660389026439 2216 42-43 -0.6990693436810886 2216 44-45 -0.7238019098519857 2216 46-47 -0.6799318112066857 2216 48-49 -0.6500912231154956 2216 50-51 -0.6671718591164009 2216 52-53 -0.6372423552138358 2216 54-55 -0.6317719066384626 2216 56-57 -0.5995921707389655 2216 58-59 -0.591697595282497 2216 60-61 -0.6394268661219726 2216 62-63 -0.6338008780150375 2216 64-65 -0.7035989228222164 2216 66-67 -0.6518188796723905 2216 68-69 -0.5975951599737357 2216 70-71 -0.6225230744290471 2216 72-73 -0.6281310932575792 2216 74-75 -0.621854871062915 2216 76-77 -0.5132243700955641 2216 78-79 -0.5233968028807254 2216 80-81 -0.5407578384396388 2216 82-83 -0.5635991498256061 2216 84-85 -0.6520111863449429 2216 86-87 -0.5824621248053781 2216 88-89 -0.6290397251782096 2216 90-91 -0.5332883899800436 2216 92-93 -0.5465040259695222 2216 94-95 -0.6035238289289353 2216 96-97 -0.5840869573695429 2216 98-99 -0.6601750389841463 2216 100 -0.6495092025074136 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 620.0 3 74.0 4 82.0 5 163.0 6 599.0 7 1628.0 8 3925.0 9 7093.0 10 8144.0 11 9924.0 12 14437.0 13 20827.0 14 25921.0 15 34356.0 16 44038.0 17 62944.0 18 65387.0 19 32660.0 20 20672.0 21 18184.0 22 18065.0 23 19425.0 24 22189.0 25 27112.0 26 34275.0 27 43915.0 28 56826.0 29 74398.0 30 97257.0 31 127003.0 32 165991.0 33 216888.0 34 292088.0 35 421566.0 36 704066.0 37 1389090.0 38 2181422.0 39 1048548.0 40 4578.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.47836104680484 21.865501329959223 15.562610045828334 22.093527577407595 2 15.272621159644522 21.38204959283143 40.653396898466184 22.69193234905787 3 17.339927669147855 26.841798813074224 31.82221262427594 23.996060893501976 4 11.954272468078477 18.59092884732614 40.10480592861497 29.349992755980413 5 11.502250412661091 38.504468359232156 36.55905923523562 13.434221992871132 6 27.96305651277055 40.2793518747837 18.072118883095484 13.685472729350263 7 24.341094913058097 34.28453688846123 23.67840380078673 17.695964397693942 8 23.68797137382148 36.50463753932956 21.03000390903698 18.777387177811978 9 24.141323988092473 16.999827783685376 23.269431057435508 35.589417170786646 10-11 22.536835156183795 27.97799594881622 29.582983661318853 19.902185233681138 12-13 23.72031660465968 25.943260191515478 29.735907648317884 20.60051555550696 14-15 20.96295572400559 27.86827638804983 28.183890120524087 22.9848777674205 16-17 18.94466935834388 31.00846320174731 29.090349325759462 20.956518114149347 18-19 18.63988065136037 28.949993029339645 32.80897793717658 19.6011483821234 20-21 20.264024011874724 28.064363797397075 32.488983623048554 19.182628567679643 22-23 19.74600143786955 28.02981802476088 32.78039822972563 19.443782307643946 24-25 19.48175873861117 28.18049363209675 32.74344689586927 19.594300733422813 26-27 19.143305842506813 28.21305071633786 32.83800185337557 19.80564158777975 28-29 19.17155751888229 28.128917579458694 33.14756751289572 19.551957388763295 30-31 19.691814804589157 28.66580877428455 32.4228511914362 19.219525229690092 32-33 19.127109308155127 28.341058009562104 32.94714735975988 19.584685322522887 34-35 19.5667939609479 28.01993608861213 32.75777091949844 19.65549903094153 36-37 19.762033956683496 28.39443823311528 32.170923325469694 19.672604484731522 38-39 19.586892698301618 28.48410689439313 32.360894322055444 19.56810608524981 40-41 19.584391461351107 28.11226316839749 32.35807872199093 19.94526664826048 42-43 19.52576957457103 28.236484436292265 32.16591401758793 20.07183197154877 44-45 19.916242732061484 28.392627228219418 31.72577832206638 19.96535171765272 46-47 20.026160478269308 28.631413349224616 31.207072349987293 20.135353822518788 48-49 19.94870414057225 28.650097452565337 30.826651978164065 20.574546428698344 50-51 20.191576982059434 28.644336406802275 30.4155401988415 20.748546412296793 52-53 20.203830309524655 28.820413920545406 30.299054997143397 20.676700772786543 54-55 20.358845494629858 29.193808960168827 30.103992684907016 20.3433528602943 56-57 20.271814749917308 28.794355951987182 30.11884975903384 20.814979539061667 58-59 20.19367501414634 28.385205525136747 30.504470790199523 20.916648670517386 60-61 20.277343440335247 28.807989743561706 30.109343691825742 20.805323124277304 62-63 20.06937173848269 28.725694947501363 30.27333872762213 20.931594586393818 64-65 20.122710958151437 28.570556750742853 30.31318083533113 20.99355145577458 66-67 19.99408860666067 28.601323332030322 30.202593359010876 21.20199470229813 68-69 20.534834166623384 28.826345815826954 30.021240012137152 20.617580005412513 70-71 20.460658139681097 28.599068118386416 29.922618836091075 21.01765490584141 72-73 20.20987154849803 28.630811958919576 30.126455979596468 21.03286051298593 74-75 20.40469467140854 28.706949338333988 29.825432796000207 21.06292319425727 76-77 20.480551857612646 28.675608702664434 29.63134090902878 21.21249853069414 78-79 20.555616302051014 28.626164852016984 29.9317285324163 20.8864903135157 80-81 20.330127005674676 28.591940315725044 29.967643772878503 21.110288905721774 82-83 20.22287929055209 28.663708355697665 30.1873191461793 20.926093207570947 84-85 20.147253813959342 28.47671112373429 29.9563908054115 21.41964425689487 86-87 20.31260720868828 28.67258866389169 29.771123203985383 21.243680923434646 88-89 20.142032871788462 28.48954623384155 29.887930903104554 21.480489991265436 90-91 19.893961761939707 28.423505905903827 29.897716380209022 21.784815951947447 92-93 20.04100124330892 28.670014823621052 29.727589396016203 21.561394537053825 94-95 19.836459682620912 28.375629633365552 29.938530917214173 21.84937976679937 96-97 19.772951754981545 28.664266875314066 29.749934068724755 21.812847300979637 98-99 19.74665799923349 28.57658048985577 29.729099886335618 21.947661624575126 100 19.83866587374147 28.497250480395397 29.648026227217354 22.01605741864578 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1682.0 1 1117.5 2 831.0 3 1122.5 4 767.5 5 574.5 6 1395.0 7 3498.5 8 5877.5 9 6839.0 10 5680.0 11 4291.0 12 4439.0 13 5219.0 14 6089.0 15 6977.5 16 7872.0 17 8986.0 18 10562.0 19 12510.5 20 15297.5 21 19093.0 22 25098.5 23 34723.5 24 45551.0 25 57945.5 26 74947.0 27 96563.5 28 124102.0 29 149946.5 30 170407.0 31 192178.0 32 214917.5 33 234750.0 34 253462.5 35 268359.0 36 274463.0 37 280506.0 38 286436.0 39 284529.0 40 284060.5 41 280947.5 42 269997.0 43 262512.5 44 252673.0 45 242483.0 46 235198.0 47 228162.5 48 222832.5 49 218380.5 50 212297.0 51 195283.5 52 172394.5 53 152782.5 54 140234.0 55 133476.0 56 117458.0 57 99236.5 58 86681.5 59 75196.5 60 60853.5 61 44370.5 62 33409.5 63 25673.5 64 19610.0 65 14735.0 66 10864.5 67 7766.5 68 5285.0 69 3694.5 70 2695.5 71 1941.5 72 1342.5 73 965.0 74 693.5 75 461.5 76 312.5 77 213.5 78 153.0 79 107.0 80 57.5 81 37.0 82 33.5 83 22.5 84 10.0 85 5.5 86 3.0 87 2.5 88 2.0 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.5 97 0.5 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13346764383479262 2 0.0 3 0.0 4 0.0 5 0.014419699359519327 6 2.1868738365147793E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.9135146069504318E-4 82-83 5.877223435633469E-4 84-85 4.510427287811732E-4 86-87 5.740543820851295E-4 88-89 6.218922472588903E-4 90-91 7.790738042583901E-4 92-93 2.255213643905866E-4 94-95 2.5969126808613005E-4 96-97 6.765640931717599E-4 98-99 0.0014214679937346064 100 1.5034757626039105E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7316380.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.8956078851173 #Duplication Level Percentage of deduplicated Percentage of total 1 70.53547529366021 38.015523184174896 2 17.016403746355834 18.3421884785687 3 6.070390538649134 9.815021645416792 4 2.659914345165854 5.734308022202296 5 1.3511740815018027 3.641117424057734 6 0.7163214639028571 2.316394844292095 7 0.43779943246166736 1.6516826580996642 8 0.2778603305782585 1.198036113893991 9 0.17325356958026822 0.8403845805715521 >10 0.6512706168703211 5.9012851951120355 >50 0.05324411509551712 2.0213800126472266 >100 0.048631924089651744 5.429677525367911 >500 0.005947738290469633 2.154165065701185 >1k 0.0022111420944486977 1.9278555497540735 >5k 2.5415425897180295E-5 0.11866402653322512 >10k+ 7.624627769154088E-5 0.8923156736065894 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25816 0.35285209352165964 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25781 0.352373714869922 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13546 0.1851462061839325 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8663 0.11840555028579708 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.366796147821737E-5 0.0 3 0.0 2.733592295643474E-5 0.0 2.733592295643474E-5 0.0 4 0.0 2.733592295643474E-5 0.0 2.733592295643474E-5 0.0 5 0.0 2.733592295643474E-5 0.0 2.733592295643474E-5 0.0 6 0.0 2.733592295643474E-5 0.0 2.733592295643474E-5 0.0 7 0.0 2.733592295643474E-5 0.0 4.100388443465211E-5 0.0 8 1.366796147821737E-5 2.733592295643474E-5 0.0 5.467184591286948E-5 0.0 9 1.366796147821737E-5 2.733592295643474E-5 0.0 1.6401553773860844E-4 0.0 10-11 1.366796147821737E-5 2.733592295643474E-5 0.0 1.9135146069504318E-4 0.0 12-13 1.366796147821737E-5 3.416990369554342E-5 0.0 2.5969126808613005E-4 0.0 14-15 2.0501942217326055E-5 4.100388443465211E-5 0.0 3.8953690212919503E-4 0.0 16-17 2.733592295643474E-5 4.100388443465211E-5 0.0 4.3737476730295585E-4 0.0 18-19 2.733592295643474E-5 9.567573034752159E-5 0.0 4.7154467099849923E-4 0.0 20-21 4.100388443465211E-5 1.0250971108663028E-4 0.0 5.740543820851294E-4 0.0 22-23 4.7837865173760795E-5 1.0934369182573896E-4 0.0 6.833980739108685E-4 0.0 24-25 5.467184591286948E-5 1.2301165330395633E-4 0.0 9.499233227361072E-4 0.0 26-27 5.467184591286948E-5 1.366796147821737E-4 0.0 0.001646989358125193 0.0 28-29 5.467184591286948E-5 1.366796147821737E-4 0.0 0.003396488427337016 0.0 30-31 5.467184591286948E-5 1.5034757626039108E-4 0.0 0.009895604110229375 0.0 32-33 5.467184591286948E-5 1.5034757626039108E-4 0.0 0.02488252387109472 0.0 34-35 5.467184591286948E-5 1.5034757626039108E-4 0.0 0.051398369138836414 0.0 36-37 5.467184591286948E-5 1.8451747995593452E-4 0.0 0.09282596037931326 0.0 38-39 5.467184591286948E-5 2.3918932586880398E-4 0.0 0.14760714998400848 0.0 40-41 5.467184591286948E-5 2.4602330660791267E-4 0.0 0.20123339684379432 0.0 42-43 5.467184591286948E-5 2.4602330660791267E-4 0.0 0.258433815630134 0.0 44-45 5.467184591286948E-5 2.8702719104256477E-4 0.0 0.33304721733972265 0.0 46-47 5.467184591286948E-5 3.143631139989995E-4 0.0 0.4259210155842097 0.0 48-49 5.467184591286948E-5 3.280310754772169E-4 0.0 0.5248633887250252 0.0 50-51 5.467184591286948E-5 4.6471069025939057E-4 0.0 0.6238946036154491 0.0 52-53 5.467184591286948E-5 5.262165169113687E-4 0.0 0.7238210699826964 0.0 54-55 5.467184591286948E-5 5.603864206069121E-4 0.0 0.8380510580368981 0.0 56-57 5.467184591286948E-5 6.08224285780673E-4 0.0 0.9788720651469716 0.0 58-59 5.467184591286948E-5 6.150582665197816E-4 0.0 1.1346253201719976 0.0 60-61 5.467184591286948E-5 6.150582665197816E-4 0.0 1.2956065704624418 0.0 62-63 5.467184591286948E-5 6.355602087371078E-4 0.0 1.4587405246857053 0.0 64-65 6.833980739108685E-5 6.423941894762164E-4 0.0 1.6368682326505732 0.0 66-67 8.200776886930422E-5 6.423941894762164E-4 0.0 1.847122484069991 0.0 68-69 8.200776886930422E-5 6.423941894762164E-4 0.0 2.0827513059737193 0.0 70-71 8.200776886930422E-5 6.423941894762164E-4 0.0 2.3356154273014793 0.0 72-73 8.200776886930422E-5 6.423941894762164E-4 0.0 2.598204303220992 0.0 74-75 8.200776886930422E-5 6.560621509544337E-4 0.0 2.868529245337175 0.0 76-77 8.200776886930422E-5 6.697301124326511E-4 0.0 3.1606204161074194 0.0 78-79 9.567573034752159E-5 6.697301124326511E-4 0.0 3.491159562515889 0.0 80-81 1.0934369182573896E-4 6.697301124326511E-4 0.0 3.8533878776116057 0.0 82-83 1.0934369182573896E-4 6.697301124326511E-4 0.0 4.226666739562461 0.0 84-85 1.2301165330395633E-4 6.765640931717597E-4 0.0 4.602262320983875 0.0 86-87 1.366796147821737E-4 6.833980739108685E-4 0.0 5.001093436918257 0.0 88 1.366796147821737E-4 6.970660353890859E-4 0.0 5.32593987737105 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15575 0.0 63.46955 1 GTATCAA 29500 0.0 40.913834 1 TGGTATC 5475 0.0 37.86263 2 GTGGTAT 5610 0.0 37.67538 1 ATCAACG 36735 0.0 32.32285 3 TCAACGC 37310 0.0 31.824705 4 TATCAAC 37890 0.0 31.48642 2 CAACGCA 39450 0.0 30.134094 5 AACGCAG 41550 0.0 28.640314 6 CGCAGAG 44870 0.0 26.196466 8 ACGCAGA 45455 0.0 25.973057 7 GCAGAGT 48105 0.0 24.043985 9 GTACATG 22720 0.0 22.74931 1 GAGTACT 36700 0.0 21.764074 12-13 TACATGG 23090 0.0 21.72185 2 CAGAGTA 48675 0.0 21.522326 10-11 ACATGGG 23260 0.0 20.593056 3 AGAGTAC 46880 0.0 20.586958 10-11 GTACTTT 39565 0.0 20.116808 14-15 AGTACTT 38490 0.0 19.188965 12-13 >>END_MODULE