FastQCFastQC Report
Wed 25 May 2016
SRR2049455_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049455_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2951489
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA65020.22029558639723884No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33750.042.225961
GTATCAA55700.033.7765661
TCAACGC55950.032.7611124
ATCAACG56250.032.757383
CAACGCA60250.030.5779515
TACCTGG27050.029.8881762
TATCAAC64450.029.0267492
AACGCAG63750.028.9011216
GTACCTG29650.028.7120741
TATAACG2850.028.0376952
TCTGTCG9950.027.3953558
GTCTCGC11000.027.3651581
ACGCAGA72400.025.188487
GTGGTAT18300.024.9305211
CGCAGAG72250.024.7182548
TCGCTCT11800.024.2963984
CGCTCTG11850.022.2100245
CTGTCGC13250.021.9915169
GTACATG68950.021.8286291
CTCGCTC12850.021.2163413
Produced by FastQC (version 0.11.5)