FastQCFastQC Report
Wed 25 May 2016
SRR2049455_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049455_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2951489
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136870.46373203491525805No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136340.4619363311196484No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80410.27243875887729885No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78470.2658658053612939No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47650.1614439355864108No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38630.13088308985735675No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29600.10028836292461196No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68850.057.1619221
TATAACG3950.051.1705972
ATAACGC4350.044.3040853
TAACGCA6000.039.171294
GTATCAA118100.038.107681
GTGGTAT25050.037.773991
TACCTGG26250.034.202132
TGGTATC29050.031.8763962
ATCAACG146650.030.2899743
TCAACGC148700.029.9040034
TATCAAC151350.029.4735812
CAACGCA154050.028.8654675
GTACCTG30600.028.7689971
GTCTCGC10700.028.5978971
CCGATCG1005.327571E-528.199039
AACGCAG162200.027.6431666
GTATAAC9500.026.2637521
ACGCGAT909.5231395E-426.1102127
GCGGTAT1901.3118552E-824.7771261
ACGCAGA182750.024.4314447
Produced by FastQC (version 0.11.5)