FastQCFastQC Report
Wed 25 May 2016
SRR2049454_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049454_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6476440
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72300.043.8354071
GTATCAA120850.034.2015461
ATCAACG129300.030.571243
TCAACGC130450.030.2285084
CAACGCA143300.027.4850585
TATCAAC147000.027.3056662
AACGCAG148150.026.5228526
GTACATG200900.024.3420331
ACGCAGA165400.023.5865767
TACATGG199450.023.1178382
CGCAGAG168050.023.0168958
ACATGGG208700.021.034883
GTGGTAT40250.020.678111
GCAGAGT199400.018.7150449
AGAGTAC175050.017.42541310-11
CATGGGG149350.017.3083744
CAGAGTA188500.017.29161610-11
TGGTATC48400.016.1203692
TATACTG35300.015.8442285
GAGTACA151450.015.3687791
Produced by FastQC (version 0.11.5)