##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049454_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6476440
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.792001778754994	31.0	31.0	34.0	28.0	34.0
2	31.00885223981076	31.0	31.0	34.0	28.0	34.0
3	31.131012871268783	31.0	31.0	34.0	28.0	34.0
4	34.7333181500948	37.0	35.0	37.0	32.0	37.0
5	34.760152645589244	37.0	35.0	37.0	32.0	37.0
6	34.76657577310992	37.0	35.0	37.0	32.0	37.0
7	34.65002717542354	37.0	35.0	37.0	32.0	37.0
8	34.62376861362107	37.0	35.0	37.0	32.0	37.0
9	36.204405506729	39.0	37.0	39.0	32.0	39.0
10-11	36.14654269938423	39.0	37.0	39.0	32.0	39.0
12-13	36.06656249420978	39.0	37.0	39.0	31.0	39.0
14-15	37.28545466336444	40.0	38.0	41.0	31.0	41.0
16-17	37.2748738504487	40.0	38.0	41.0	31.0	41.0
18-19	37.29556392091952	40.0	38.0	41.0	31.0	41.0
20-21	37.3390250662401	40.0	38.0	41.0	31.0	41.0
22-23	37.29808644872801	40.0	38.0	41.0	31.0	41.0
24-25	37.298568194872495	40.0	38.0	41.0	31.0	41.0
26-27	37.29176530624849	40.0	38.0	41.0	31.0	41.0
28-29	37.26583362155752	40.0	38.0	41.0	31.0	41.0
30-31	37.20889995120776	40.0	37.0	41.0	31.0	41.0
32-33	37.15680628555194	40.0	37.0	41.0	31.0	41.0
34-35	37.08630706066913	40.0	37.0	41.0	31.0	41.0
36-37	36.97757209207528	40.0	37.0	41.0	30.0	41.0
38-39	36.869817291598466	40.0	37.0	41.0	30.0	41.0
40-41	36.74569277257258	40.0	37.0	41.0	30.0	41.0
42-43	36.649809926441065	40.0	37.0	41.0	30.0	41.0
44-45	36.499919863381734	40.0	36.0	41.0	30.0	41.0
46-47	36.37265156474854	40.0	36.0	41.0	30.0	41.0
48-49	36.26532632125057	40.0	36.0	41.0	29.5	41.0
50-51	36.14876807320071	40.0	36.0	41.0	29.0	41.0
52-53	36.03332633360303	39.5	35.0	41.0	29.0	41.0
54-55	35.8862263064276	39.0	35.0	41.0	28.5	41.0
56-57	35.71781804509885	39.0	35.0	41.0	28.0	41.0
58-59	35.53384953770899	39.0	35.0	41.0	28.0	41.0
60-61	35.33706334035365	39.0	35.0	40.5	28.0	41.0
62-63	35.08614887191111	38.0	34.5	40.0	27.5	41.0
64-65	34.845485096750686	38.0	34.0	40.0	27.0	41.0
66-67	34.62071678267691	37.0	34.0	40.0	27.0	41.0
68-69	34.33590753562142	37.0	34.0	40.0	26.0	41.0
70-71	34.02519679021191	36.5	34.0	39.0	26.0	41.0
72-73	33.691537480467666	36.0	34.0	39.0	26.0	41.0
74-75	33.38903093366109	35.5	33.0	39.0	26.0	40.5
76-77	33.06717208836954	35.0	33.0	38.0	26.0	40.0
78-79	32.72497475464915	35.0	33.0	37.0	25.0	39.0
80-81	32.397349006552986	35.0	33.0	37.0	25.0	39.0
82-83	32.105842793262966	35.0	33.0	36.0	24.5	39.0
84-85	31.827020477299257	35.0	33.0	36.0	24.0	37.0
86-87	31.562430362977192	35.0	32.0	36.0	24.0	37.0
88-89	31.343442539419804	35.0	32.0	35.0	23.0	37.0
90-91	31.14587443101457	35.0	32.0	35.0	22.5	36.0
92-93	30.94731511447647	35.0	32.0	35.0	20.0	36.0
94-95	30.753365120343894	35.0	32.0	35.0	19.5	36.0
96-97	30.569474433485063	35.0	32.0	35.0	17.5	36.0
98-99	30.400556246950487	35.0	32.0	35.0	6.0	35.0
100	30.269860448023913	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.26025476092410216
1101	2	0.294555618632554
1101	3	0.3752018579940035
1101	4	0.45507756200985483
1101	5	0.43561993370099117
1101	6	0.45888296525126293
1101	7	0.4876123734578144
1101	8	0.49692057221164987
1101	9	0.6105739584332923
1101	10-11	0.6014137961987203
1101	12-13	0.6432903423135201
1101	14-15	0.7062759232310114
1101	16-17	0.6154541206292734
1101	18-19	0.6516044496390592
1101	20-21	0.6771202090188027
1101	22-23	0.7007626383510868
1101	24-25	0.7056707821508894
1101	26-27	0.6932617148417677
1101	28-29	0.7266184089044003
1101	30-31	0.7226002077953027
1101	32-33	0.6850116894964202
1101	34-35	0.6695502627856769
1101	36-37	0.6929250700930538
1101	38-39	0.6239548853574135
1101	40-41	0.6430340414944169
1101	42-43	0.623045188010316
1101	44-45	0.6477487726914077
1101	46-47	0.5708098797599561
1101	48-49	0.5691780757103473
1101	50-51	0.5498408445836915
1101	52-53	0.5253617509810056
1101	54-55	0.5281504959096992
1101	56-57	0.5099162941685051
1101	58-59	0.47394811788866775
1101	60-61	0.5027302322626852
1101	62-63	0.43427587659635947
1101	64-65	0.5059964010180664
1101	66-67	0.5016336062802438
1101	68-69	0.4390916825325064
1101	70-71	0.4771017547724625
1101	72-73	0.49946609063265157
1101	74-75	0.4611443081886648
1101	76-77	0.4808618255788062
1101	78-79	0.47245054882218085
1101	80-81	0.4948047865037779
1101	82-83	0.46877335709675805
1101	84-85	0.4875428585371253
1101	86-87	0.4208852751231156
1101	88-89	0.39571746028449795
1101	90-91	0.36338714587617815
1101	92-93	0.3870495802901921
1101	94-95	0.38358464380229407
1101	96-97	0.3740103283111864
1101	98-99	0.4635724115422519
1101	100	0.42911400520964804
1102	1	0.1398625863160845
1102	2	0.1927054766949503
1102	3	0.24918262736980878
1102	4	0.25514075976103356
1102	5	0.26417353580974634
1102	6	0.2750161862330387
1102	7	0.2631149234835064
1102	8	0.2910133609008341
1102	9	0.4209524233791768
1102	10-11	0.4134942486379529
1102	12-13	0.37314630117556646
1102	14-15	0.4612029222356995
1102	16-17	0.45594194183864545
1102	18-19	0.4520092933527877
1102	20-21	0.5291410338039739
1102	22-23	0.4282256486594278
1102	24-25	0.4789748637207225
1102	26-27	0.4158915138095267
1102	28-29	0.44821623782062403
1102	30-31	0.34707149608161814
1102	32-33	0.3622301945235975
1102	34-35	0.3844274832423906
1102	36-37	0.380318937923839
1102	38-39	0.3775678124314297
1102	40-41	0.43708634428350024
1102	42-43	0.3612705429766194
1102	44-45	0.32718082965642026
1102	46-47	0.26787860377280737
1102	48-49	0.22590735207622714
1102	50-51	0.2800493798369459
1102	52-53	0.190698192123115
1102	54-55	0.2734545136804911
1102	56-57	0.2778475986919773
1102	58-59	0.21870400905849863
1102	60-61	0.2137927585950763
1102	62-63	0.17814479787668347
1102	64-65	0.16094259069610928
1102	66-67	0.24955203480970312
1102	68-69	0.18346218054627172
1102	70-71	0.16549829197190036
1102	72-73	0.20607509151707148
1102	74-75	0.22666821764781986
1102	76-77	0.1433206848721369
1102	78-79	0.15902595940373487
1102	80-81	0.1976688543680325
1102	82-83	0.1619760725719388
1102	84-85	0.14455551072487083
1102	86-87	0.11381205460199695
1102	88-89	0.15310051896597088
1102	90-91	0.13679125742953957
1102	92-93	0.1254102789326197
1102	94-95	0.08069326770158369
1102	96-97	0.13731230328932043
1102	98-99	0.19897465845110673
1102	100	0.21110041417706427
1103	1	0.18866793864427933
1103	2	0.2406700254564953
1103	3	0.2693483999671109
1103	4	0.2638618794041747
1103	5	0.3044424543813733
1103	6	0.31917466279190876
1103	7	0.3197827301561844
1103	8	0.2978953855121844
1103	9	0.3936566257263081
1103	10-11	0.4478463149234031
1103	12-13	0.4625390981065891
1103	14-15	0.5278696626096604
1103	16-17	0.5446674175823176
1103	18-19	0.499539558792371
1103	20-21	0.4855503858825543
1103	22-23	0.4867513580060532
1103	24-25	0.4928989314587042
1103	26-27	0.48332326209227006
1103	28-29	0.4310688031043952
1103	30-31	0.4293482109184481
1103	32-33	0.47515715011299164
1103	34-35	0.43691507953481334
1103	36-37	0.4518595233660605
1103	38-39	0.4889079361712234
1103	40-41	0.5360419120580815
1103	42-43	0.46518807287844055
1103	44-45	0.4899048199526135
1103	46-47	0.44392647567151045
1103	48-49	0.4829736694686346
1103	50-51	0.43417605415934446
1103	52-53	0.46560637741536937
1103	54-55	0.36626423291961885
1103	56-57	0.32150624158362007
1103	58-59	0.3379946182098905
1103	60-61	0.3890828336143528
1103	62-63	0.42148181903117177
1103	64-65	0.513386748828701
1103	66-67	0.4273804290178873
1103	68-69	0.3632596525829541
1103	70-71	0.3742260207015704
1103	72-73	0.4214022388436831
1103	74-75	0.3871262003187468
1103	76-77	0.39038353556525607
1103	78-79	0.4312696632046169
1103	80-81	0.3982118682195903
1103	82-83	0.3592473674664376
1103	84-85	0.28243849991889647
1103	86-87	0.31663619835675405
1103	88-89	0.29663253274235046
1103	90-91	0.25874073403410947
1103	92-93	0.23507709989319991
1103	94-95	0.3091753963876229
1103	96-97	0.3298375984010029
1103	98-99	0.3227903912219041
1103	100	0.2563928036050811
1104	1	0.115861069557468
1104	2	0.1329344363308067
1104	3	0.1795551559041968
1104	4	0.17027810786235165
1104	5	0.24167604864468473
1104	6	0.20893291464875574
1104	7	0.2121754104046687
1104	8	0.2446597504496424
1104	9	0.3351827741741715
1104	10-11	0.3392068312371279
1104	12-13	0.3181739519006186
1104	14-15	0.3652556091877486
1104	16-17	0.3412466906148168
1104	18-19	0.3292099833963178
1104	20-21	0.35207659624786203
1104	22-23	0.3457883045627952
1104	24-25	0.37969606075331797
1104	26-27	0.3937687599327617
1104	28-29	0.33110835951124784
1104	30-31	0.3054595436874834
1104	32-33	0.3304369692338298
1104	34-35	0.32442956674449874
1104	36-37	0.3314147402298744
1104	38-39	0.36445361468094717
1104	40-41	0.3690869439726967
1104	42-43	0.31318457153495416
1104	44-45	0.303233719210084
1104	46-47	0.25507260781797925
1104	48-49	0.2802188971367485
1104	50-51	0.2538774799472634
1104	52-53	0.2241890090863663
1104	54-55	0.23483398458110116
1104	56-57	0.2705589281465137
1104	58-59	0.3002833396451621
1104	60-61	0.28707266287668176
1104	62-63	0.3099565044050081
1104	64-65	0.31784034735900946
1104	66-67	0.3245204972638973
1104	68-69	0.29361082124833615
1104	70-71	0.28152462593134686
1104	72-73	0.24476653601396237
1104	74-75	0.2795690631883403
1104	76-77	0.2363253772686278
1104	78-79	0.2218074380989421
1104	80-81	0.23207722382449703
1104	82-83	0.2558452275285106
1104	84-85	0.1796414350184321
1104	86-87	0.21069782521010794
1104	88-89	0.14248772539349375
1104	90-91	0.1390635150217001
1104	92-93	0.12006653441702753
1104	94-95	0.1399747176779904
1104	96-97	0.2054139584827155
1104	98-99	0.23486160549155244
1104	100	0.20972915772384582
1105	1	0.12626871223716307
1105	2	0.14105687979655812
1105	3	0.2056828563811237
1105	4	0.22025025619652183
1105	5	0.23401721335441295
1105	6	0.23012377886523439
1105	7	0.2320841747836937
1105	8	0.24481657081563668
1105	9	0.2821594360912627
1105	10-11	0.37136701595548516
1105	12-13	0.37087424071259534
1105	14-15	0.4257698062745874
1105	16-17	0.3355228440165945
1105	18-19	0.37502335830435385
1105	20-21	0.3216651589615438
1105	22-23	0.28169456537116133
1105	24-25	0.30833696013542067
1105	26-27	0.3398327935722776
1105	28-29	0.3915580628565465
1105	30-31	0.39114795726131746
1105	32-33	0.3364991475754948
1105	34-35	0.32037805521575535
1105	36-37	0.3297254720670182
1105	38-39	0.34976394273069644
1105	40-41	0.3566420822512981
1105	42-43	0.3856591275842405
1105	44-45	0.40508978204240975
1105	46-47	0.3362873038123766
1105	48-49	0.29555293732286003
1105	50-51	0.3792892173619933
1105	52-53	0.34638538827760357
1105	54-55	0.2736924001628722
1105	56-57	0.28159676079798146
1105	58-59	0.28252565299690957
1105	60-61	0.2476957531296904
1105	62-63	0.3190073708824954
1105	64-65	0.2812639549689493
1105	66-67	0.27249871608074017
1105	68-69	0.27240785627336095
1105	70-71	0.2886394336591138
1105	72-73	0.2969430145231726
1105	74-75	0.33097568643319875
1105	76-77	0.2961775387845478
1105	78-79	0.2389995708118704
1105	80-81	0.28700203161226057
1105	82-83	0.26559997566181437
1105	84-85	0.22030610793999728
1105	86-87	0.19763987167239705
1105	88-89	0.17982227640691661
1105	90-91	0.23118363541502518
1105	92-93	0.23654072332270815
1105	94-95	0.24289561879905008
1105	96-97	0.24222424384967667
1105	98-99	0.28354112416550237
1105	100	0.2573057770685807
1106	1	0.11918081584113693
1106	2	0.2007278547720901
1106	3	0.23672809388875038
1106	4	0.3196209456943535
1106	5	0.3141445655784594
1106	6	0.2637718170469654
1106	7	0.3545885924711598
1106	8	0.29240813207810135
1106	9	0.35921075215696874
1106	10-11	0.3541665918610164
1106	12-13	0.36402100572036034
1106	14-15	0.3930835205434775
1106	16-17	0.33431215164785755
1106	18-19	0.35269906922778205
1106	20-21	0.31588980025396296
1106	22-23	0.32657194142395696
1106	24-25	0.36718372702127056
1106	26-27	0.3738942198180837
1106	28-29	0.37296227356672773
1106	30-31	0.4064097933616537
1106	32-33	0.37574838075700256
1106	34-35	0.42520238220672013
1106	36-37	0.39968715583086833
1106	38-39	0.36200084994474935
1106	40-41	0.44319584053260996
1106	42-43	0.3655218098279036
1106	44-45	0.29791965661368636
1106	46-47	0.3603229205000318
1106	48-49	0.31045038199011543
1106	50-51	0.33777141977527236
1106	52-53	0.2996603581422761
1106	54-55	0.2730503850343311
1106	56-57	0.2365168679087759
1106	58-59	0.2328095744485239
1106	60-61	0.270951132497963
1106	62-63	0.3549987055932604
1106	64-65	0.3076970174260225
1106	66-67	0.37359841187508636
1106	68-69	0.37715036974685745
1106	70-71	0.3189490328502558
1106	72-73	0.329889482229234
1106	74-75	0.316522308521364
1106	76-77	0.2320595808640462
1106	78-79	0.2496132627906178
1106	80-81	0.2945225488068317
1106	82-83	0.28035293234480463
1106	84-85	0.30327727620836953
1106	86-87	0.347576891906602
1106	88-89	0.3293228017416183
1106	90-91	0.2813366221738214
1106	92-93	0.15493325512295897
1106	94-95	0.21283720793740457
1106	96-97	0.2816669340923923
1106	98-99	0.30305079899143195
1106	100	0.2899712374405041
1107	1	0.1407646017260582
1107	2	0.13189456987026915
1107	3	0.20619029655386711
1107	4	0.14407826117972888
1107	5	0.16832216078411477
1107	6	0.2112558629524841
1107	7	0.23836004228416385
1107	8	0.16675147626763476
1107	9	0.2737964363055241
1107	10-11	0.2416968301063136
1107	12-13	0.2595014022800939
1107	14-15	0.2968827367077722
1107	16-17	0.28553611656582945
1107	18-19	0.29187302263540715
1107	20-21	0.30597321252437126
1107	22-23	0.30098170597133844
1107	24-25	0.3003232399638023
1107	26-27	0.39131541168612216
1107	28-29	0.3306218899891604
1107	30-31	0.2938553550854053
1107	32-33	0.27746865859033676
1107	34-35	0.30144564110648275
1107	36-37	0.2671481150012127
1107	38-39	0.3000947649342365
1107	40-41	0.3926967915513728
1107	42-43	0.3612627459020885
1107	44-45	0.3149004833961797
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2214	4	-0.2716615310976209
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2214	84-85	-0.506239698193717
2214	86-87	-0.5016243106283191
2214	88-89	-0.5310918324924749
2214	90-91	-0.48138951519776185
2214	92-93	-0.46872948429855654
2214	94-95	-0.4597884734521891
2214	96-97	-0.3966502276860382
2214	98-99	-0.4286339683333509
2214	100	-0.49039987031279964
2215	1	-0.40432650929477987
2215	2	-0.5410591049586522
2215	3	-0.5893227178695284
2215	4	-0.528297921658357
2215	5	-0.5204168538530425
2215	6	-0.5376949493619279
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2215	16-17	-0.951554566142697
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2215	22-23	-0.8210103958865389
2215	24-25	-0.871400959642358
2215	26-27	-0.9521319029416162
2215	28-29	-0.8360484412587539
2215	30-31	-1.102476796773793
2215	32-33	-1.0727133347025841
2215	34-35	-0.8859281280379179
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2215	40-41	-0.8971686430535044
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2215	44-45	-0.8256001529591686
2215	46-47	-0.7742796006165591
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2215	64-65	-0.7648521546628899
2215	66-67	-0.7098720936676415
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2215	80-81	-0.637983326575096
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2215	84-85	-0.6178717098034632
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2215	90-91	-0.6172910493357513
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2215	94-95	-0.6309566475206019
2215	96-97	-0.6817533658219652
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2215	100	-0.7310187094391658
2216	1	-0.44717503216945786
2216	2	-0.6106882149919279
2216	3	-0.602350427428032
2216	4	-0.7239608688079286
2216	5	-0.6991063665708808
2216	6	-0.7392339046328686
2216	7	-0.650401012837186
2216	8	-0.6104106997915935
2216	9	-0.7694020931062369
2216	10-11	-0.7701070828924514
2216	12-13	-0.8023205074278437
2216	14-15	-0.9524391730001582
2216	16-17	-0.9594183774695466
2216	18-19	-1.096624509244478
2216	20-21	-1.0525694396763683
2216	22-23	-1.0246655328192844
2216	24-25	-1.048861123466878
2216	26-27	-1.0745711833365093
2216	28-29	-1.0342421063086604
2216	30-31	-1.1464919814016312
2216	32-33	-1.1535460987111747
2216	34-35	-1.1662824029083225
2216	36-37	-1.1484890970514385
2216	38-39	-1.1097192262216637
2216	40-41	-1.1112367748821939
2216	42-43	-1.0005353824401624
2216	44-45	-1.130206339927554
2216	46-47	-0.9989842380389504
2216	48-49	-1.176884345478861
2216	50-51	-1.138579506854768
2216	52-53	-1.2184073177395902
2216	54-55	-1.191784741051137
2216	56-57	-1.1533120489582274
2216	58-59	-1.1648487069624522
2216	60-61	-1.1155083251383004
2216	62-63	-1.1268059845897298
2216	64-65	-1.1254055527304772
2216	66-67	-1.1570019198964872
2216	68-69	-1.0610288114335376
2216	70-71	-1.052863185051919
2216	72-73	-1.092169494614211
2216	74-75	-1.1437716558116549
2216	76-77	-1.1421516184196676
2216	78-79	-1.1471032871529587
2216	80-81	-1.109156374805984
2216	82-83	-1.0988490221025344
2216	84-85	-1.0004458785038892
2216	86-87	-1.1537350929582395
2216	88-89	-1.1292973566011177
2216	90-91	-1.1467058182176793
2216	92-93	-1.1660856392988634
2216	94-95	-1.0815721190753003
2216	96-97	-1.114869471858647
2216	98-99	-1.1952960924131695
2216	100	-1.2989616355418825
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	106459.0
3	20312.0
4	12647.0
5	10049.0
6	8600.0
7	8663.0
8	9439.0
9	10577.0
10	12278.0
11	13814.0
12	15117.0
13	16861.0
14	19027.0
15	20536.0
16	20645.0
17	22291.0
18	21591.0
19	18863.0
20	18836.0
21	20169.0
22	23369.0
23	27448.0
24	33415.0
25	41529.0
26	50829.0
27	58449.0
28	70621.0
29	88330.0
30	106052.0
31	124463.0
32	159048.0
33	210969.0
34	291882.0
35	426100.0
36	713691.0
37	1281537.0
38	1722614.0
39	638009.0
40	1311.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.65279443215111	20.136229339992333	14.385407266388933	22.825568961467617
2	15.300439361516652	20.31767886905989	40.388737112450826	23.99314465697264
3	18.22323564431664	25.608210263292413	30.282349416475814	25.88620467591513
4	12.71796126190066	17.255592143665883	38.94757502364226	31.0788715707912
5	13.195261694393157	37.89756566623176	35.3969391043632	13.510233535011876
6	30.44496469037895	38.0673122609538	18.005428353348528	13.482294695318728
7	26.69750831862857	32.703584399988515	22.691223441467525	17.907683839915396
8	24.602859268114948	36.27634845942303	20.872169272526722	18.2486229999353
9	24.733151066538706	17.296727613573808	21.91083648413993	36.059284835747555
10-11	23.56145319442985	27.289179180969985	29.103143504250976	20.046224120349184
12-13	24.09911875054533	24.876862124959366	29.07035247661751	21.9536666478778
14-15	21.613237198148834	27.071240415197384	26.27609682585727	25.039425560796513
16-17	21.169729146589887	30.31298810167888	26.913088338542714	21.60419441318852
18-19	21.08477386170727	29.281465175423282	28.839750066921265	20.79401089594818
20-21	22.175853302905274	28.43003631168191	28.44143293550159	20.95267744991122
22-23	21.89869473018391	28.35107378533188	28.386584030257602	21.363647454226605
24-25	21.641344699096017	28.49376516395487	28.377426136260976	21.487464000688135
26-27	21.459129484261354	28.451978013553102	28.472646379241272	21.616246122944272
28-29	21.451268389148716	28.468771069170344	28.58988550107525	21.490075040605685
30-31	21.748435688319145	28.78981292036015	28.166636206975493	21.295115184345217
32-33	21.418862037779913	28.5826242904327	28.50189157463464	21.49662209715274
34-35	21.631620030899203	28.28535669586984	28.528844275578898	21.554178997652063
36-37	21.857974338129477	28.5533612902633	28.126263117212876	21.462401254394344
38-39	21.6721907640551	28.678327654802132	28.2014901009472	21.447991480195565
40-41	21.664095241610234	28.41032963913932	28.373182829170844	21.5523922900796
42-43	21.613473375765256	28.47330936564904	28.278434241690242	21.634783016895458
44-45	21.68326834896567	28.58342829900232	28.161391548330872	21.571911803701134
46-47	21.76405630855098	28.571878634250393	28.035624369670746	21.62844068752788
48-49	21.667046955431317	28.501341820915265	28.055126041595486	21.776485182057936
50-51	21.669082972122887	28.493734862380997	28.026687995320422	21.810494170175694
52-53	21.680241806871223	28.477172477335237	28.040373414864217	21.802212300929323
54-55	21.80327263815626	28.6825209286481	27.901145097520835	21.613061335674807
56-57	21.67551219976254	28.52980938990686	27.97574996818635	21.81892844214425
58-59	21.68354541202673	28.24422960611251	28.163898132434134	21.908326849426622
60-61	21.82537286628852	28.556144621177857	27.903239679054032	21.715242833479593
62-63	21.684994129781064	28.608346945575096	27.958943599955678	21.747715324688162
64-65	21.804318864941184	28.373335236053084	27.994883969617025	21.82746192938871
66-67	21.632589526520192	28.418756662457568	27.936974985217706	22.01167882580454
68-69	21.912278367743596	28.516051151378562	27.93584681858189	21.63582366229595
70-71	21.857701368770485	28.35475875779283	27.950896868535573	21.836643004901106
72-73	21.756988911206566	28.480216630070547	28.033804743215097	21.728989715507787
74-75	21.776882532254437	28.526201027629362	27.9210222951604	21.775894144955803
76-77	21.85583651026212	28.448864520767604	27.721146053896195	21.974152915074086
78-79	21.894557142934353	28.513844374422366	27.929810284083583	21.6617881985597
80-81	21.74347036995626	28.426229589211356	27.98530466459066	21.844995376241727
82-83	21.727996496438376	28.537488480002764	28.077738670870282	21.656776352688574
84-85	21.77418869155246	28.38346117194583	27.909708584790966	21.932641551710745
86-87	21.89520873424245	28.59893933752531	27.776617829945916	21.729234098286327
88-89	21.689694330568788	28.45760298206697	27.952144607639696	21.90055807972455
90-91	21.669496817855833	28.383186307545294	27.942989183242457	22.004327691356416
92-93	21.763829155108528	28.54410993527442	27.85280659720356	21.839254312413487
94-95	21.654568492447297	28.38482617931139	28.003449929310424	21.95715539893089
96-97	21.735373517616114	28.493868089115875	27.88250308871398	21.888255304554033
98-99	21.638831358708106	28.56636247905287	27.810899239448606	21.983906922790414
100	21.701005019305914	28.43155376123669	27.811741670584983	22.05569954887241
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2866.0
1	2188.0
2	1737.5
3	2037.0
4	1890.0
5	1564.5
6	1435.0
7	1385.5
8	1403.0
9	1424.5
10	1306.0
11	1170.0
12	1183.0
13	1261.0
14	1362.0
15	1597.0
16	1871.5
17	2221.0
18	2866.0
19	3884.0
20	5565.0
21	7816.0
22	11081.0
23	16575.5
24	23921.5
25	33434.0
26	46395.5
27	61675.5
28	79737.0
29	100769.5
30	120542.5
31	138968.5
32	157286.5
33	172455.0
34	185001.0
35	196197.0
36	206303.5
37	215185.0
38	223371.5
39	227918.0
40	230662.0
41	232830.0
42	230505.5
43	226877.0
44	223224.5
45	219858.5
46	217585.5
47	212196.5
48	206144.5
49	202054.5
50	195518.5
51	186080.0
52	174517.0
53	164442.5
54	157848.0
55	151633.5
56	138705.5
57	123017.5
58	111653.5
59	101971.5
60	90580.0
61	77533.5
62	65931.0
63	55676.5
64	46035.0
65	37450.0
66	30004.5
67	23806.0
68	18955.0
69	14846.5
70	11094.5
71	8305.5
72	6402.0
73	4826.5
74	3640.0
75	2614.5
76	1757.0
77	1207.5
78	829.5
79	555.5
80	387.5
81	275.0
82	195.5
83	151.5
84	119.5
85	99.5
86	77.5
87	57.0
88	46.0
89	32.0
90	27.5
91	23.0
92	17.0
93	14.0
94	15.5
95	13.5
96	13.0
97	13.0
98	8.5
99	11.5
100	27.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07720290777031825
2	0.014004607469535732
3	0.017447857156091928
4	0.011549555002439612
5	0.009619482308181656
6	0.006840177628450198
7	0.018744866006633273
8	0.009063621372235363
9	0.009032740209127237
10-11	0.012607234838892973
12-13	0.015054567015212062
14-15	0.009704405506729005
16-17	0.012020492739838554
18-19	0.008438277819295787
20-21	0.01293920734230534
22-23	0.013031850831629724
24-25	0.014961923525887679
26-27	0.005550889068685883
28-29	0.011518673839331485
30-31	0.010322028768891552
32-33	0.011588156456324772
34-35	0.008415116946964691
36-37	0.009966895393148089
38-39	0.018127242744470726
40-41	0.01208997535683184
42-43	0.0076894096139236996
44-45	0.0199646719494043
46-47	0.017810710822612423
48-49	0.01694603825558486
50-51	0.009951454811594023
52-53	0.010708043307743144
54-55	0.011534114420885548
56-57	0.019022796474606418
58-59	0.002300646651555484
60-61	0.007087226933315215
62-63	0.014784356838015947
64-65	0.023129991167987352
66-67	0.01218261884615622
68-69	0.013533669732136792
70-71	0.02436523769231244
72-73	0.019362489268795822
74-75	0.019231244325586278
76-77	0.009017299627573173
78-79	0.010885609995614874
80-81	0.01834341088622762
82-83	0.011487792676223357
84-85	0.010762085343182366
86-87	0.016258932376429027
88-89	0.014382901717610292
90-91	0.014390622008387323
92-93	0.007473241472166808
94-95	0.01459134956859015
96-97	0.013101333448623008
98-99	0.009449635911086956
100	0.006871058791558325
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6476440.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.42091601601312
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.62719008479355	43.01367888438759
2	15.445985317853312	18.047372220777604
3	5.00949850844953	8.779784749334189
4	2.135279615789452	4.989799644189614
5	1.0981971367321157	3.2078841347026508
6	0.6327368605519458	2.217904019432462
7	0.4107861690889385	1.6798952999419252
8	0.2834693283322513	1.3248430258891282
9	0.21433304843410667	1.1269379719822517
>10	1.0670616534057322	10.998797320594566
>50	0.054520771085860786	2.1677493253330438
>100	0.020094296466390268	1.9850907439187941
>500	6.883634987814749E-4	0.29095087199846703
>1k	1.5884551795779348E-4	0.16931178751769746
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.0881163108127304E-5	0.0
3	0.0	0.0	0.0	4.632174466219096E-5	0.0
4	0.0	0.0	0.0	4.632174466219096E-5	0.0
5	0.0	0.0	0.0	6.176232621625461E-5	0.0
6	0.0	0.0	0.0	6.176232621625461E-5	0.0
7	0.0	0.0	0.0	6.176232621625461E-5	0.0
8	0.0	0.0	0.0	7.720290777031826E-5	0.0
9	0.0	0.0	0.0	9.264348932438192E-5	0.0
10-11	0.0	0.0	0.0	1.2352465243250921E-4	0.0
12-13	0.0	0.0	0.0	1.5440581554063652E-4	0.0
14-15	0.0	7.720290777031826E-6	0.0	1.6212610631766833E-4	0.0
16-17	0.0	1.5440581554063652E-5	0.0	1.6984639709470017E-4	0.0
18-19	0.0	1.5440581554063652E-5	0.0	2.0072756020282748E-4	0.0
20-21	0.0	1.5440581554063652E-5	0.0	2.2388843253392295E-4	0.0
22-23	0.0	1.5440581554063652E-5	0.0	2.9337104952720944E-4	0.0
24-25	0.0	1.5440581554063652E-5	0.0	3.474130849664322E-4	0.0
26-27	0.0	1.5440581554063652E-5	0.0	5.867420990544188E-4	0.0
28-29	0.0	1.5440581554063652E-5	0.0	0.0012506871058791558	0.0
30-31	0.0	1.5440581554063652E-5	0.0	0.0028719481690558397	0.0
32-33	1.5440581554063652E-5	1.5440581554063652E-5	0.0	0.006315197855612034	0.0
34-35	3.0881163108127304E-5	2.3160872331095476E-5	0.0	0.012244381172372476	0.0
36-37	3.0881163108127304E-5	3.860145388515913E-5	0.0	0.021084114112073917	0.0
38-39	3.0881163108127304E-5	4.632174466219096E-5	0.0	0.031244016774647804	0.0
40-41	3.0881163108127304E-5	6.948261699328644E-5	0.0	0.04219138909647893	0.0
42-43	3.0881163108127304E-5	9.264348932438192E-5	0.0	0.05371778322658745	0.0
44-45	4.632174466219096E-5	9.264348932438192E-5	0.0	0.06783047476700163	0.0
46-47	4.632174466219096E-5	1.0808407087844556E-4	0.0	0.08508532465366775	0.0
48-49	4.632174466219096E-5	1.1580436165547738E-4	0.0	0.103336092050571	1.5440581554063652E-5
50-51	4.632174466219096E-5	1.2352465243250921E-4	0.0	0.12177986671690003	1.5440581554063652E-5
52-53	4.632174466219096E-5	1.2352465243250921E-4	0.0	0.1394593325963029	1.5440581554063652E-5
54-55	4.632174466219096E-5	1.2352465243250921E-4	0.0	0.1599798654816535	1.5440581554063652E-5
56-57	4.632174466219096E-5	1.2352465243250921E-4	0.0	0.18596636423714263	1.5440581554063652E-5
58-59	4.632174466219096E-5	1.5440581554063652E-4	0.0	0.2150950213388837	1.5440581554063652E-5
60-61	4.632174466219096E-5	1.5440581554063652E-4	0.0	0.24578317717758522	1.5440581554063652E-5
62-63	4.632174466219096E-5	1.5440581554063652E-4	0.0	0.2754213734706104	1.5440581554063652E-5
64-65	4.632174466219096E-5	1.5440581554063652E-4	0.0	0.30746830048606955	1.5440581554063652E-5
66-67	4.632174466219096E-5	1.5440581554063652E-4	0.0	0.3466873776333912	1.5440581554063652E-5
68-69	4.632174466219096E-5	1.5440581554063652E-4	0.0	0.3911794133814256	1.5440581554063652E-5
70-71	4.632174466219096E-5	1.5440581554063652E-4	0.0	0.4395624756810841	1.5440581554063652E-5
72-73	4.632174466219096E-5	1.5440581554063652E-4	0.0	0.49094101080223085	1.5440581554063652E-5
74-75	4.632174466219096E-5	1.5440581554063652E-4	0.0	0.5445429896671629	1.5440581554063652E-5
76-77	4.632174466219096E-5	1.6212610631766833E-4	0.0	0.6055255665149373	1.5440581554063652E-5
78-79	4.632174466219096E-5	1.6984639709470017E-4	0.0	0.6775558794646441	1.5440581554063652E-5
80-81	4.632174466219096E-5	1.6984639709470017E-4	0.0	0.7571443570850652	1.5440581554063652E-5
82-83	4.632174466219096E-5	1.6984639709470017E-4	0.0	0.842708339766909	1.5440581554063652E-5
84-85	4.632174466219096E-5	1.6984639709470017E-4	0.0	0.9294149254837534	1.5440581554063652E-5
86-87	4.632174466219096E-5	1.6984639709470017E-4	0.0	1.0235252700557713	1.5440581554063652E-5
88	4.632174466219096E-5	1.6984639709470017E-4	0.0	1.1021487113290636	1.5440581554063652E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7230	0.0	43.835407	1
GTATCAA	12085	0.0	34.201546	1
ATCAACG	12930	0.0	30.57124	3
TCAACGC	13045	0.0	30.228508	4
CAACGCA	14330	0.0	27.485058	5
TATCAAC	14700	0.0	27.305666	2
AACGCAG	14815	0.0	26.522852	6
GTACATG	20090	0.0	24.342033	1
ACGCAGA	16540	0.0	23.586576	7
TACATGG	19945	0.0	23.117838	2
CGCAGAG	16805	0.0	23.016895	8
ACATGGG	20870	0.0	21.03488	3
GTGGTAT	4025	0.0	20.67811	1
GCAGAGT	19940	0.0	18.715044	9
AGAGTAC	17505	0.0	17.425413	10-11
CATGGGG	14935	0.0	17.308374	4
CAGAGTA	18850	0.0	17.291616	10-11
TGGTATC	4840	0.0	16.120369	2
TATACTG	3530	0.0	15.844228	5
GAGTACA	15145	0.0	15.368779	1
>>END_MODULE