FastQCFastQC Report
Wed 25 May 2016
SRR2049454_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049454_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6476440
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212900.32872998128601516No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201500.31112771831438263No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119530.18456127131572284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122450.059.8810961
GTATCAA226200.040.9670031
ATCAACG277900.032.8476943
TCAACGC279950.032.657534
CAACGCA292100.031.3152225
TATCAAC292800.031.2885152
GTGGTAT45000.030.7479321
AACGCAG302600.030.2867646
ACGCAGA342200.026.6720497
CGCAGAG345500.026.2268528
TGGTATC52250.025.3692132
GCAGAGT373150.023.8804269
GTACATG193450.022.6740071
TACATGG191700.021.798362
GAGTACT279350.021.5435912-13
CAGAGTA368100.021.5203910-11
AGAGTAC354350.020.67100310-11
ACATGGG198850.020.2581333
GTACTTT303400.019.74292614-15
AGTACTT287950.019.35774612-13
Produced by FastQC (version 0.11.5)