##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049454_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6476440
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.959751344874654	33.0	31.0	34.0	30.0	34.0
2	32.14836098226804	33.0	31.0	34.0	30.0	34.0
3	32.17797153992008	34.0	31.0	34.0	30.0	34.0
4	35.83678780317582	37.0	35.0	37.0	35.0	37.0
5	35.886950392499585	37.0	35.0	37.0	35.0	37.0
6	35.95497310250693	37.0	35.0	37.0	35.0	37.0
7	35.91746561382488	37.0	35.0	37.0	35.0	37.0
8	35.936799383611984	37.0	35.0	37.0	35.0	37.0
9	37.664685073898625	39.0	37.0	39.0	35.0	39.0
10-11	37.63578038243232	39.0	37.0	39.0	35.0	39.0
12-13	37.61096265849757	39.0	37.0	39.0	35.0	39.0
14-15	38.91465133622793	40.0	38.0	41.0	35.0	41.0
16-17	38.925526832642625	40.0	38.0	41.0	35.5	41.0
18-19	39.0036314703757	40.0	38.5	41.0	36.0	41.0
20-21	39.02740973436023	40.0	39.0	41.0	35.0	41.0
22-23	38.99618810642884	40.0	39.0	41.0	35.0	41.0
24-25	38.96961501998011	40.0	38.5	41.0	35.0	41.0
26-27	38.868761541834715	40.0	38.0	41.0	35.0	41.0
28-29	38.78667122678509	40.0	38.0	41.0	35.0	41.0
30-31	38.67419986906387	40.0	38.0	41.0	35.0	41.0
32-33	38.537755464421814	40.0	38.0	41.0	34.5	41.0
34-35	38.42537767662481	40.0	38.0	41.0	34.0	41.0
36-37	38.291834788865486	40.0	38.0	41.0	34.0	41.0
38-39	38.184192781836934	40.0	38.0	41.0	34.0	41.0
40-41	38.09175449784141	40.0	38.0	41.0	34.0	41.0
42-43	37.94696330082576	40.0	38.0	41.0	33.0	41.0
44-45	37.76412481239693	40.0	37.5	41.0	33.0	41.0
46-47	37.51190854852357	40.0	37.0	41.0	33.0	41.0
48-49	37.25903343503529	40.0	37.0	41.0	32.0	41.0
50-51	37.097834998857394	40.0	37.0	41.0	32.0	41.0
52-53	36.981379353471965	40.0	37.0	41.0	31.5	41.0
54-55	36.83588329390838	40.0	36.0	41.0	31.0	41.0
56-57	36.68342623416568	39.5	36.0	41.0	31.0	41.0
58-59	36.50597689471377	39.0	35.0	41.0	31.0	41.0
60-61	36.3221614034871	39.0	35.0	41.0	31.0	41.0
62-63	36.10448749621706	39.0	35.0	41.0	30.5	41.0
64-65	35.88654484562507	38.5	35.0	40.5	30.0	41.0
66-67	35.62693833340539	38.0	35.0	40.0	30.0	41.0
68-69	35.3349852696852	37.0	35.0	40.0	30.0	41.0
70-71	35.051679317649814	37.0	35.0	39.5	29.0	41.0
72-73	34.72392116656682	36.0	34.5	39.0	29.0	41.0
74-75	34.396552967370965	36.0	34.0	39.0	29.0	41.0
76-77	34.08083437814602	35.5	34.0	38.5	29.0	40.0
78-79	33.77423468757527	35.0	34.0	37.0	29.0	39.0
80-81	33.46853973788069	35.0	34.0	37.0	29.0	39.0
82-83	33.19425309274848	35.0	34.0	36.5	29.0	39.0
84-85	32.91475463371852	35.0	34.0	36.0	28.5	37.5
86-87	32.6713804188721	35.0	34.0	36.0	28.0	37.0
88-89	32.455828973942474	35.0	34.0	35.5	27.0	37.0
90-91	32.266957773097566	35.0	33.0	35.0	27.0	36.0
92-93	32.10647825039682	35.0	33.0	35.0	27.0	36.0
94-95	31.950658463600373	35.0	33.0	35.0	27.0	36.0
96-97	31.80745540760047	35.0	33.0	35.0	26.5	36.0
98-99	31.675770485019548	35.0	33.0	35.0	26.0	35.5
100	31.569550246740494	35.0	33.0	35.0	26.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3113794619772463
1101	2	0.3509613615144431
1101	3	0.43141143240682567
1101	4	0.26673843232382666
1101	5	0.2536605255282751
1101	6	0.22749697218148413
1101	7	0.25153330823826536
1101	8	0.2448551950531268
1101	9	0.3219324822608556
1101	10-11	0.2995544074445817
1101	12-13	0.29499958143572513
1101	14-15	0.42854530918920375
1101	16-17	0.3547953110813111
1101	18-19	0.35175913473972287
1101	20-21	0.39222966461213105
1101	22-23	0.425783618348305
1101	24-25	0.3913296770786374
1101	26-27	0.4177786942592334
1101	28-29	0.44057091268865634
1101	30-31	0.4054826889446588
1101	32-33	0.4260291463352033
1101	34-35	0.4491126427413974
1101	36-37	0.480934697205484
1101	38-39	0.4392576050783177
1101	40-41	0.48351948263749733
1101	42-43	0.4682746072738908
1101	44-45	0.4680871582197881
1101	46-47	0.4300938211409999
1101	48-49	0.3602638926104902
1101	50-51	0.29793314395603687
1101	52-53	0.29072108147694564
1101	54-55	0.3002060580202297
1101	56-57	0.3216548027485757
1101	58-59	0.27163606649199323
1101	60-61	0.21689869940959028
1101	62-63	0.26436483189655746
1101	64-65	0.24386545649143443
1101	66-67	0.22216875473686315
1101	68-69	0.1748396441847575
1101	70-71	0.1863517391947198
1101	72-73	0.18411420449066895
1101	74-75	0.16008724765760007
1101	76-77	0.20835751496634458
1101	78-79	0.2223332044981845
1101	80-81	0.2356954996417926
1101	82-83	0.22468245164321132
1101	84-85	0.22003960480923013
1101	86-87	0.15525565911980976
1101	88-89	0.20691540739456116
1101	90-91	0.18232149485393734
1101	92-93	0.20519234902911165
1101	94-95	0.20444128322231947
1101	96-97	0.1874762363948932
1101	98-99	0.19733594853764203
1101	100	0.2159308109199074
1102	1	0.23003505899878718
1102	2	0.24725355628132917
1102	3	0.32808988661083305
1102	4	0.24340459145170712
1102	5	0.2314945334397649
1102	6	0.178795262509567
1102	7	0.19879732767231673
1102	8	0.164937913683616
1102	9	0.1984817240203327
1102	10-11	0.22598446931335303
1102	12-13	0.2189838261273067
1102	14-15	0.2945494135269371
1102	16-17	0.3456379559168852
1102	18-19	0.34794663179691554
1102	20-21	0.32555585877037174
1102	22-23	0.24040963768084112
1102	24-25	0.2418948184587677
1102	26-27	0.23776559201299818
1102	28-29	0.2489466886981333
1102	30-31	0.30561031762357516
1102	32-33	0.30449118763174
1102	34-35	0.2642583033153514
1102	36-37	0.3107666498906383
1102	38-39	0.3478340984092867
1102	40-41	0.35206153569099286
1102	42-43	0.2757322272757321
1102	44-45	0.31878343926500463
1102	46-47	0.2875828922410619
1102	48-49	0.3124382733571167
1102	50-51	0.29721188853744707
1102	52-53	0.28175221887951807
1102	54-55	0.2804383395660537
1102	56-57	0.29380343354549865
1102	58-59	0.33845856692971665
1102	60-61	0.2782403160204936
1102	62-63	0.28619142016172816
1102	64-65	0.2681729463167102
1102	66-67	0.27085257825279285
1102	68-69	0.2681821927102561
1102	70-71	0.2333776026782033
1102	72-73	0.2624043552419266
1102	74-75	0.24040768094489096
1102	76-77	0.22397179792309174
1102	78-79	0.23273109961866112
1102	80-81	0.2369529065996403
1102	82-83	0.2976763638275841
1102	84-85	0.24530614420392283
1102	86-87	0.2548715602895797
1102	88-89	0.20693375216994525
1102	90-91	0.22651469570945437
1102	92-93	0.16614381303390502
1102	94-95	0.16785989020568692
1102	96-97	0.19068817725378295
1102	98-99	0.21587329309879522
1102	100	0.16332568975164818
1103	1	0.2721867073147948
1103	2	0.273333871669891
1103	3	0.36423878908478713
1103	4	0.22907363493261812
1103	5	0.18352671698859524
1103	6	0.18459565728578298
1103	7	0.203812641589181
1103	8	0.18651842390509188
1103	9	0.27888762512382925
1103	10-11	0.24590713071633985
1103	12-13	0.21750719895300819
1103	14-15	0.3344710110881195
1103	16-17	0.31373863466269825
1103	18-19	0.2927078496771287
1103	20-21	0.2764103964610882
1103	22-23	0.2806694165491592
1103	24-25	0.30182123647636416
1103	26-27	0.3072238483836358
1103	28-29	0.3515218246757925
1103	30-31	0.39525675360609114
1103	32-33	0.39240160822024706
1103	34-35	0.42074992500829467
1103	36-37	0.39515765819155746
1103	38-39	0.4065827231541945
1103	40-41	0.4055722013807923
1103	42-43	0.4021633156325848
1103	44-45	0.4065400818883518
1103	46-47	0.4137636829433333
1103	48-49	0.4190010717180712
1103	50-51	0.41759711229065033
1103	52-53	0.45617930443943067
1103	54-55	0.40577916205090503
1103	56-57	0.4351129515637311
1103	58-59	0.4374594401153473
1103	60-61	0.35205607336038724
1103	62-63	0.4412351482368848
1103	64-65	0.3691456736385774
1103	66-67	0.41642159112060284
1103	68-69	0.4165867739619742
1103	70-71	0.39971440467635944
1103	72-73	0.4170251386324253
1103	74-75	0.3859083794442597
1103	76-77	0.35954055035850274
1103	78-79	0.3526587485479098
1103	80-81	0.34724865953674566
1103	82-83	0.33628399390742914
1103	84-85	0.32720899112322854
1103	86-87	0.2692843441793329
1103	88-89	0.31019474671708025
1103	90-91	0.35203052385747924
1103	92-93	0.39101117677493846
1103	94-95	0.39380223784166546
1103	96-97	0.39368308850283995
1103	98-99	0.4415220523349319
1103	100	0.3603048458228919
1104	1	0.16542677212880363
1104	2	0.16995931153442
1104	3	0.2921722522364192
1104	4	0.1713828799395003
1104	5	0.12853530346504272
1104	6	0.14934009565379824
1104	7	0.15565744555321714
1104	8	0.12886354565308977
1104	9	0.1331780387335897
1104	10-11	0.1716638062138074
1104	12-13	0.16864556016432886
1104	14-15	0.24222430270835105
1104	16-17	0.20507903075277056
1104	18-19	0.16607977743632318
1104	20-21	0.19459121806676194
1104	22-23	0.18610507305282908
1104	24-25	0.18270523725427523
1104	26-27	0.16334520824185716
1104	28-29	0.20551122511553643
1104	30-31	0.21323221566065342
1104	32-33	0.233383254589981
1104	34-35	0.22220857635654312
1104	36-37	0.2099203533627403
1104	38-39	0.22804430290310762
1104	40-41	0.18578168522393668
1104	42-43	0.2043920136996178
1104	44-45	0.20809506499904273
1104	46-47	0.19096726316375623
1104	48-49	0.20806853709235895
1104	50-51	0.237864504873599
1104	52-53	0.29287678201676215
1104	54-55	0.19562990678812753
1104	56-57	0.24213656664428385
1104	58-59	0.25648699762751903
1104	60-61	0.18089816038661155
1104	62-63	0.16367505322131137
1104	64-65	0.10740498062777704
1104	66-67	0.13945557342353965
1104	68-69	0.2027261335185031
1104	70-71	0.1534933034679824
1104	72-73	0.16627619102273172
1104	74-75	0.1929419897707234
1104	76-77	0.13673501397114762
1104	78-79	0.13311794842582003
1104	80-81	0.13826132949868253
1104	82-83	0.10204561218668573
1104	84-85	0.08182608121288837
1104	86-87	0.12987139121335645
1104	88-89	0.13857583177266974
1104	90-91	0.10442587422240024
1104	92-93	0.1373702890367028
1104	94-95	0.11225920538901946
1104	96-97	0.0780928164540633
1104	98-99	0.10783751338230729
1104	100	0.1131131561121208
1105	1	0.17409742662718486
1105	2	0.17472188776564224
1105	3	0.29582838884125096
1105	4	0.19462347376550326
1105	5	0.15744362847156168
1105	6	0.12230897053358802
1105	7	0.16928919474700166
1105	8	0.09823998650992394
1105	9	0.18734981051446908
1105	10-11	0.2019791887492275
1105	12-13	0.2044730886340389
1105	14-15	0.28757756989549677
1105	16-17	0.2811487358117546
1105	18-19	0.24795027079312604
1105	20-21	0.2410299449198945
1105	22-23	0.22231258582980473
1105	24-25	0.24479351193462406
1105	26-27	0.15816680077157486
1105	28-29	0.22349719262974332
1105	30-31	0.21936020254876354
1105	32-33	0.1934461867303483
1105	34-35	0.22661418590390525
1105	36-37	0.2070482502863129
1105	38-39	0.19022328546109435
1105	40-41	0.14324663118778602
1105	42-43	0.15269680500917815
1105	44-45	0.20546479495508407
1105	46-47	0.22990090721452106
1105	48-49	0.24636050512681607
1105	50-51	0.2200989976975194
1105	52-53	0.21310794452524107
1105	54-55	0.26407920187227063
1105	56-57	0.22483097366801275
1105	58-59	0.25271282455528166
1105	60-61	0.29156380876818844
1105	62-63	0.2805874193575235
1105	64-65	0.23092828255070685
1105	66-67	0.24404281615278478
1105	68-69	0.2685255673965301
1105	70-71	0.25662410095361565
1105	72-73	0.2934959673094184
1105	74-75	0.3099173640896993
1105	76-77	0.2803161939471934
1105	78-79	0.22171682208995946
1105	80-81	0.23736127098359816
1105	82-83	0.21601344714117232
1105	84-85	0.18408705841937234
1105	86-87	0.2089713857458193
1105	88-89	0.19103590971407414
1105	90-91	0.18231385769452402
1105	92-93	0.19308778757473988
1105	94-95	0.17575724120142056
1105	96-97	0.23851339146366968
1105	98-99	0.23154818923266518
1105	100	0.19624636874067392
1106	1	0.1679208532396146
1106	2	0.13070422045451835
1106	3	0.23916292264991057
1106	4	0.12299226190086898
1106	5	0.12438054095837003
1106	6	0.08098270673462338
1106	7	0.09024109099983235
1106	8	0.0520275831442234
1106	9	0.12418089160091483
1106	10-11	0.11789608737700519
1106	12-13	0.09822092123161497
1106	14-15	0.16140171137048043
1106	16-17	0.133697912976956
1106	18-19	0.1385104512426807
1106	20-21	0.17423237662527669
1106	22-23	0.16651994962391115
1106	24-25	0.1495576784912913
1106	26-27	0.16603478018424056
1106	28-29	0.19107448904468782
1106	30-31	0.21077420288611393
1106	32-33	0.19814359200191234
1106	34-35	0.1562392132706023
1106	36-37	0.1451619288186876
1106	38-39	0.15682916346087694
1106	40-41	0.22601481420071678
1106	42-43	0.1721146807259899
1106	44-45	0.243535017427007
1106	46-47	0.23291832486381026
1106	48-49	0.2563755906444811
1106	50-51	0.2708538422965887
1106	52-53	0.2878564299556672
1106	54-55	0.33357818131394623
1106	56-57	0.3129083282330498
1106	58-59	0.2919432217807909
1106	60-61	0.25330033313510114
1106	62-63	0.2753258061144379
1106	64-65	0.2618415195744461
1106	66-67	0.21324688227689137
1106	68-69	0.2403656258164233
1106	70-71	0.28428927630633893
1106	72-73	0.29191196701351174
1106	74-75	0.25861982572982356
1106	76-77	0.22949580925899937
1106	78-79	0.20032045458808057
1106	80-81	0.17903770060510737
1106	82-83	0.18839217642901218
1106	84-85	0.17112221700423902
1106	86-87	0.18588590471672006
1106	88-89	0.14070033872648935
1106	90-91	0.14205258039054058
1106	92-93	0.1849154845816443
1106	94-95	0.13184372824029467
1106	96-97	0.18250974625980376
1106	98-99	0.17328672570732095
1106	100	0.15343315555891834
1107	1	0.10238499945496926
1107	2	0.1149638221588063
1107	3	0.22213751686303596
1107	4	0.09485795691821153
1107	5	0.16307700369692668
1107	6	0.08891635430669709
1107	7	0.09778036769723286
1107	8	0.10353779716118083
1107	9	0.11433860221194436
1107	10-11	0.10703956667159531
1107	12-13	0.11484888986309016
1107	14-15	0.1420791895857363
1107	16-17	0.13707624673170926
1107	18-19	0.13974238849054643
1107	20-21	0.14316387685737197
1107	22-23	0.14183332351391442
1107	24-25	0.11529293487009795
1107	26-27	0.15456665440823514
1107	28-29	0.16508917511415433
1107	30-31	0.1568038882574001
1107	32-33	0.1111621708326922
1107	34-35	0.1425548741342908
1107	36-37	0.13015602269027937
1107	38-39	0.15590361841768896
1107	40-41	0.20573042373958117
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2216	60-61	-0.5350822191143223
2216	62-63	-0.5762317111793891
2216	64-65	-0.6093056986956782
2216	66-67	-0.5558571964897325
2216	68-69	-0.5596530784959839
2216	70-71	-0.5108839378917764
2216	72-73	-0.5073348011266035
2216	74-75	-0.5800117851714219
2216	76-77	-0.46767409028964835
2216	78-79	-0.5079796607040663
2216	80-81	-0.4989020193821361
2216	82-83	-0.4982255012926231
2216	84-85	-0.6372727996763317
2216	86-87	-0.5431104556080584
2216	88-89	-0.5462496471686222
2216	90-91	-0.5227147808909223
2216	92-93	-0.5431931874734985
2216	94-95	-0.5518786139075722
2216	96-97	-0.5331003822786826
2216	98-99	-0.585260915096292
2216	100	-0.6577130815013774
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	420.0
3	45.0
4	65.0
5	164.0
6	502.0
7	1382.0
8	3404.0
9	5662.0
10	5981.0
11	7007.0
12	9865.0
13	14199.0
14	17362.0
15	22410.0
16	28507.0
17	40512.0
18	41600.0
19	21998.0
20	15504.0
21	14520.0
22	15211.0
23	16553.0
24	19436.0
25	23659.0
26	30442.0
27	39673.0
28	52164.0
29	68402.0
30	89155.0
31	116560.0
32	151925.0
33	200562.0
34	275996.0
35	407793.0
36	684775.0
37	1313993.0
38	1920075.0
39	796487.0
40	2470.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.43062452904689	20.540868294532107	14.917892987084947	23.110614189336058
2	15.412927472500323	20.77391282865278	39.89733557324703	23.915824125599865
3	17.73230354948089	25.76497273193298	30.92822600070409	25.574497717882043
4	12.552081081581857	17.81012408051337	38.31932357900328	31.318471258901496
5	12.6656766885479	37.720652594893664	35.49964142119513	14.114029295363306
6	29.98050929672422	38.47642085034311	17.49407581749241	14.048994035440254
7	26.014477089265093	33.25350037983831	22.584815114476473	18.147207416420134
8	24.734329353780783	35.95844321880539	20.407044610928228	18.9001828164856
9	24.665958458659386	16.717224277535188	21.879118775129548	36.73769848867588
10-11	23.540286021332708	27.201386873035187	28.82269425795653	20.435632847675574
12-13	24.443158587125026	24.80580380579454	29.148467367874943	21.60257023920549
14-15	21.658658151700624	26.647039114081196	27.11450272063047	24.57980001358771
16-17	20.504459239952812	29.93382166745928	27.74293438988086	21.818784702707042
18-19	20.34403931789687	28.12833902576107	30.90911828103094	20.61850337531113
20-21	21.39683221028837	27.287661122468513	30.82312196206558	20.492384705177535
22-23	21.129617505913743	27.14799334202123	30.87339649560561	20.848992656459416
24-25	20.942724706783356	27.313145802323497	30.79537523701293	20.948754253880217
26-27	20.766925965499563	27.29290319990612	30.872948718740545	21.06722211585377
28-29	20.76457127681257	27.28512886709365	31.012647380350934	20.937652475742848
30-31	21.075729876290062	27.500054042035437	30.643223746379185	20.780992335295316
32-33	20.715840801427944	27.392278782788075	30.92520736701027	20.96667304877371
34-35	20.970100857878712	27.13966314827282	30.86361488719111	21.026621106657363
36-37	21.206241391875782	27.408468232547513	30.382262477533956	21.003027898042752
38-39	21.077984201196955	27.533382537319884	30.43222047915213	20.956412782331032
40-41	21.031469449265337	27.365458492628665	30.45548171526332	21.147590342842673
42-43	21.062319731210355	27.41065307483741	30.294791583030182	21.23223561092205
44-45	21.2093295081866	27.54798160717925	30.02649603794677	21.216192846687377
46-47	21.36459227600348	27.649511151188	29.618424628345203	21.367471944463315
48-49	21.358964184027027	27.744717777050354	29.293253701107396	21.60306433781522
50-51	21.44877432663624	27.798088147191972	29.073982002458145	21.679155523713646
52-53	21.462879297885877	27.909422460487555	28.97244473815862	21.655253503467957
54-55	21.552511873807216	28.154726979636962	28.825489003217818	21.467272143338008
56-57	21.4361825323789	27.91522811915188	28.910149711878745	21.738439636590474
58-59	21.40374959082459	27.605721661900674	29.190712799006864	21.799815948267877
60-61	21.490332651889002	27.87894275249983	28.96259364712713	21.668130948484045
62-63	21.3662675791021	27.875545824557936	29.07413640827368	21.684050188066283
64-65	21.41324554848034	27.68803540216539	29.164818943740695	21.733900105613575
66-67	21.328129342663562	27.80413313487039	29.03681652265751	21.830920999808537
68-69	21.665313042350427	27.868273310645975	28.900700075967663	21.56571357103594
70-71	21.625368257870065	27.723595061484396	28.930847811451972	21.720188869193567
72-73	21.502121535905527	27.86364885647053	28.94185694610002	21.692372661523923
74-75	21.59375366713812	27.953814132455484	28.740334195947153	21.71209800445924
76-77	21.682112395081248	27.886091741759362	28.58806690095176	21.84372896220763
78-79	21.721022660597487	27.98842419600892	28.716138804651937	21.574414338741654
80-81	21.550847882898356	27.88804008384255	28.780075427415586	21.7810366058435
82-83	21.535832823212086	27.923098961668757	28.909256166406607	21.631812048712547
84-85	21.577404848607625	27.841293703183982	28.717040412475047	21.864261035733342
86-87	21.653776801516152	28.00934654189376	28.609741266992835	21.727135389597255
88-89	21.469441242863223	27.906867402656772	28.701938677201944	21.921752677278064
90-91	21.419505539809485	27.85866552940864	28.72871732982356	21.993111600958308
92-93	21.483588613335332	28.006090712489527	28.636076300201747	21.874244373973394
94-95	21.32972990249784	27.820545315116586	28.77485333140428	22.074871450981295
96-97	21.372520590557304	27.96389223566026	28.67394819120711	21.989638982575325
98-99	21.289163875296936	28.017317751227715	28.603534346181007	22.089984027294342
100	21.414716802487295	27.86536541559753	28.577717305541906	22.142200476373265
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1320.0
1	972.0
2	1115.5
3	1629.0
4	1075.0
5	686.5
6	1783.0
7	4886.5
8	8307.5
9	9548.0
10	7250.5
11	4329.0
12	3801.0
13	4129.5
14	4753.0
15	5482.5
16	6200.0
17	7016.0
18	7955.0
19	9076.0
20	10730.5
21	12855.0
22	16016.5
23	21134.0
24	28125.0
25	37109.5
26	49050.0
27	63511.0
28	81077.5
29	101997.5
30	121562.5
31	139443.5
32	156550.5
33	170870.0
34	183602.0
35	194441.0
36	203885.5
37	213089.5
38	221147.0
39	225469.5
40	228722.5
41	230939.0
42	229242.0
43	226181.5
44	222534.5
45	219025.0
46	215573.5
47	209883.5
48	203682.0
49	198945.5
50	192609.5
51	183715.5
52	171699.5
53	161108.5
54	154181.5
55	146072.5
56	133204.0
57	118564.0
58	107771.0
59	100089.0
60	89318.5
61	75288.5
62	64030.5
63	54438.0
64	44276.5
65	35510.0
66	28403.0
67	22360.5
68	17541.0
69	13540.0
70	10044.0
71	7329.5
72	5515.5
73	4086.5
74	3060.0
75	2119.5
76	1291.5
77	819.5
78	567.5
79	352.0
80	196.0
81	105.0
82	62.5
83	47.0
84	27.5
85	10.5
86	10.0
87	9.0
88	4.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	1.0
98	1.0
99	1.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1262113136229163
2	0.0
3	0.0
4	0.0
5	0.013525949441359758
6	2.9337104952720944E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	2.316087233109548E-4
82-83	5.944623898314506E-4
84-85	3.7057395729752767E-4
86-87	5.249797728381642E-4
88-89	6.099029713855143E-4
90-91	6.793855883788007E-4
92-93	3.3969279418940033E-4
94-95	3.010913403042412E-4
96-97	7.56588496149119E-4
98-99	0.0012275262335480605
100	9.264348932438192E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6476440.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.40845713670313
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.57210352286411	39.65699830230295
2	16.489469328404656	18.273121089797804
3	5.464338375546177	9.083116759856768
4	2.3579638170148316	5.2260454833985275
5	1.1835977764927041	3.2790663332946566
6	0.6935472735909114	2.3057030628623596
7	0.442215027151756	1.7151716664000858
8	0.32558363303259186	1.4432069420238756
9	0.2301547808259679	1.1477269177382652
>10	1.1502894265462789	11.33066970438739
>50	0.0649480947385219	2.4505682766796806
>100	0.02403174466215996	2.3242791752399588
>500	0.001143595331321166	0.42985637609420796
>1k	5.020394716270043E-4	0.42449096796830166
>5k	2.7891081613967606E-5	0.08484255882569931
>10k+	8.367324484190281E-5	0.8251363831294286
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21290	0.32872998128601516	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20150	0.31112771831438263	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11953	0.18456127131572284	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.5440581554063652E-5	0.0	0.0	0.0
5	0.0	1.5440581554063652E-5	0.0	0.0	0.0
6	0.0	1.5440581554063652E-5	0.0	0.0	0.0
7	0.0	1.5440581554063652E-5	0.0	0.0	0.0
8	0.0	1.5440581554063652E-5	0.0	0.0	0.0
9	0.0	1.5440581554063652E-5	0.0	0.0	0.0
10-11	0.0	2.3160872331095476E-5	0.0	0.0	0.0
12-13	0.0	3.0881163108127304E-5	0.0	0.0	0.0
14-15	0.0	3.0881163108127304E-5	0.0	1.5440581554063652E-5	0.0
16-17	0.0	3.0881163108127304E-5	0.0	3.0881163108127304E-5	0.0
18-19	0.0	3.0881163108127304E-5	0.0	3.0881163108127304E-5	0.0
20-21	0.0	3.0881163108127304E-5	0.0	4.632174466219095E-5	0.0
22-23	0.0	3.0881163108127304E-5	0.0	1.0808407087844557E-4	0.0
24-25	0.0	3.860145388515913E-5	0.0	1.4668552476360472E-4	0.0
26-27	0.0	4.632174466219096E-5	0.0	3.7057395729752767E-4	0.0
28-29	0.0	6.176232621625461E-5	0.0	0.0010190783825682012	0.0
30-31	0.0	9.264348932438192E-5	0.0	0.002617178573413789	0.0
32-33	0.0	9.264348932438192E-5	0.0	0.006014106515307793	0.0
34-35	0.0	9.264348932438192E-5	0.0	0.011989611576730426	0.0
36-37	0.0	9.264348932438192E-5	0.0	0.020860225679539993	0.0
38-39	0.0	9.264348932438192E-5	0.0	0.031089610959107165	0.0
40-41	0.0	9.264348932438192E-5	0.0	0.04208330502560048	0.0
42-43	0.0	9.264348932438192E-5	0.0	0.05370234264503339	0.0
44-45	0.0	9.264348932438192E-5	0.0	0.06786135593010975	0.0
46-47	0.0	9.264348932438192E-5	0.0	0.08515480727066105	0.0
48-49	0.0	9.264348932438192E-5	0.0	0.10354453990155085	0.0
50-51	0.0	9.264348932438192E-5	0.0	0.12192655224166363	0.0
52-53	0.0	9.264348932438192E-5	0.0	0.13968322102883685	0.0
54-55	0.0	1.0036378010141374E-4	0.0	0.1601960336234104	0.0
56-57	0.0	1.0808407087844556E-4	0.0	0.18624429470511578	0.0
58-59	0.0	1.0808407087844556E-4	0.0	0.2153883923884109	0.0
60-61	0.0	1.0808407087844556E-4	0.0	0.24641624102130183	0.0
62-63	0.0	1.0808407087844556E-4	0.0	0.27635552865463126	0.0
64-65	0.0	1.0808407087844556E-4	0.0	0.3083638542162052	0.0
66-67	0.0	1.0808407087844556E-4	0.0	0.3476832951436283	0.0
68-69	0.0	1.1580436165547738E-4	0.0	0.3923220164164263	0.0
70-71	0.0	1.2352465243250921E-4	0.0	0.44068191784375366	0.0
72-73	0.0	1.2352465243250921E-4	0.0	0.4922920616882114	0.0
74-75	0.0	1.2352465243250921E-4	0.0	0.5457241941560487	0.0
76-77	0.0	1.2352465243250921E-4	0.0	0.6068148550747015	0.0
78-79	0.0	1.2352465243250921E-4	0.0	0.6790767767477195	0.0
80-81	0.0	1.2352465243250921E-4	0.0	0.7589354645453367	0.0
82-83	0.0	1.2352465243250921E-4	0.0	0.8446924544966061	0.0
84-85	0.0	1.2352465243250921E-4	0.0	0.9316846909722007	0.0
86-87	0.0	1.2352465243250921E-4	0.0	1.0262119312461784	0.0
88	0.0	1.2352465243250921E-4	0.0	1.1053912334554168	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12245	0.0	59.881096	1
GTATCAA	22620	0.0	40.967003	1
ATCAACG	27790	0.0	32.847694	3
TCAACGC	27995	0.0	32.65753	4
CAACGCA	29210	0.0	31.315222	5
TATCAAC	29280	0.0	31.288515	2
GTGGTAT	4500	0.0	30.747932	1
AACGCAG	30260	0.0	30.286764	6
ACGCAGA	34220	0.0	26.672049	7
CGCAGAG	34550	0.0	26.226852	8
TGGTATC	5225	0.0	25.369213	2
GCAGAGT	37315	0.0	23.880426	9
GTACATG	19345	0.0	22.674007	1
TACATGG	19170	0.0	21.79836	2
GAGTACT	27935	0.0	21.54359	12-13
CAGAGTA	36810	0.0	21.52039	10-11
AGAGTAC	35435	0.0	20.671003	10-11
ACATGGG	19885	0.0	20.258133	3
GTACTTT	30340	0.0	19.742926	14-15
AGTACTT	28795	0.0	19.357746	12-13
>>END_MODULE