FastQCFastQC Report
Wed 25 May 2016
SRR2049453_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049453_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6586309
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA97580.1481558183802187No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76850.050.731051
GTATCAA141750.035.5991671
ATCAACG157050.030.8270173
TCAACGC158150.030.4920964
TATCAAC176250.027.8422012
CAACGCA182750.026.439795
TGGTATC37650.025.842682
GTGGTAT41600.025.0970571
AACGCAG198200.024.4726986
CGCAGAG193650.024.4135118
ACGCAGA204500.023.3970057
GTACATG224300.023.3780751
TACATGG216200.022.7409292
ACATGGG221900.021.0976583
GCAGAGT219300.020.916269
ATACCGT6100.018.4921446
TACCGCG4850.018.410338
AGAGTAC208900.018.18930410-11
CAGAGTA223100.018.000610-11
GGTACCG5050.017.6834936
Produced by FastQC (version 0.11.5)