##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049453_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6586309
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.029864678380562	31.0	31.0	34.0	28.0	34.0
2	31.265455811441583	31.0	31.0	34.0	28.0	34.0
3	31.341382100353933	33.0	31.0	34.0	28.0	34.0
4	34.916009710446325	37.0	35.0	37.0	32.0	37.0
5	34.92157246190545	37.0	35.0	37.0	32.0	37.0
6	34.99728208925515	37.0	35.0	37.0	32.0	37.0
7	34.91077035711504	37.0	35.0	37.0	32.0	37.0
8	34.89214414325232	37.0	35.0	37.0	32.0	37.0
9	36.43677179433883	39.0	37.0	39.0	32.0	39.0
10-11	36.38852550039787	39.0	37.0	39.0	32.0	39.0
12-13	36.30361140055834	39.0	37.0	39.0	32.0	39.0
14-15	37.54943656606454	40.0	38.0	41.0	32.0	41.0
16-17	37.5611211833517	40.0	38.0	41.0	32.0	41.0
18-19	37.607455480755604	40.0	38.0	41.0	32.0	41.0
20-21	37.67143023505274	40.0	38.0	41.0	32.0	41.0
22-23	37.64712686574529	40.0	38.0	41.0	32.0	41.0
24-25	37.66811282313053	40.0	38.0	41.0	32.0	41.0
26-27	37.67959527863026	40.0	38.0	41.0	32.0	41.0
28-29	37.6728129518369	40.0	38.0	41.0	32.0	41.0
30-31	37.614453634046015	40.0	38.0	41.0	32.0	41.0
32-33	37.559563634199364	40.0	38.0	41.0	32.0	41.0
34-35	37.4866641543845	40.0	38.0	41.0	32.0	41.0
36-37	37.36685706668181	40.0	38.0	41.0	31.5	41.0
38-39	37.25697913656951	40.0	37.5	41.0	31.0	41.0
40-41	37.13431612455474	40.0	37.0	41.0	31.0	41.0
42-43	37.04314556149734	40.0	37.0	41.0	30.5	41.0
44-45	36.90364109852726	40.0	37.0	41.0	30.0	41.0
46-47	36.78794124296324	40.0	37.0	41.0	30.0	41.0
48-49	36.68752878129465	40.0	37.0	41.0	30.0	41.0
50-51	36.580640234158466	40.0	36.5	41.0	30.0	41.0
52-53	36.47123502708421	40.0	36.0	41.0	30.0	41.0
54-55	36.34006337995985	40.0	36.0	41.0	30.0	41.0
56-57	36.19373347044604	39.5	35.5	41.0	29.5	41.0
58-59	36.029062256265235	39.0	35.0	41.0	29.0	41.0
60-61	35.8544307593221	39.0	35.0	41.0	29.0	41.0
62-63	35.62561329873834	39.0	35.0	41.0	28.5	41.0
64-65	35.407167884166995	38.5	35.0	40.5	28.0	41.0
66-67	35.1990497257265	38.0	35.0	40.0	28.0	41.0
68-69	34.93271178136343	37.5	34.0	40.0	28.0	41.0
70-71	34.632079894824244	37.0	34.0	40.0	28.0	41.0
72-73	34.298593795098284	36.5	34.0	39.5	27.0	41.0
74-75	33.99582565895405	36.0	34.0	39.0	27.0	41.0
76-77	33.66312398947575	36.0	34.0	39.0	26.0	40.5
78-79	33.298411674884974	35.0	34.0	38.5	26.0	40.0
80-81	32.93973157044408	35.0	33.0	37.0	26.0	39.0
82-83	32.61334125076731	35.0	33.0	37.0	26.0	39.0
84-85	32.28932836282051	35.0	33.0	36.5	25.0	38.5
86-87	31.981735445452074	35.0	33.0	36.0	25.0	37.0
88-89	31.713439955519853	35.0	33.0	36.0	24.5	37.0
90-91	31.4641311241243	35.0	33.0	35.0	24.0	36.5
92-93	31.227242754629337	35.0	33.0	35.0	23.0	36.0
94-95	30.993722811972532	35.0	32.0	35.0	20.0	36.0
96-97	30.785905884464274	35.0	32.0	35.0	18.5	36.0
98-99	30.5930955410686	35.0	32.0	35.0	9.5	36.0
100	30.446967641512114	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.32898815042032226
1101	2	0.3197205273699488
1101	3	0.3759607320214471
1101	4	0.36213839241189305
1101	5	0.327429765484915
1101	6	0.37481351940915886
1101	7	0.434461394616072
1101	8	0.4539670671260225
1101	9	0.534371384598245
1101	10-11	0.5273884388049197
1101	12-13	0.545089407582644
1101	14-15	0.6410551899099346
1101	16-17	0.6258694720652471
1101	18-19	0.6089418525708581
1101	20-21	0.6547588434610816
1101	22-23	0.5907817562506565
1101	24-25	0.5742867893845798
1101	26-27	0.6230310782201514
1101	28-29	0.587424905331396
1101	30-31	0.5780419509554875
1101	32-33	0.5641829855201195
1101	34-35	0.5672052093431432
1101	36-37	0.5651853401718796
1101	38-39	0.5423922019094576
1101	40-41	0.5776148353626525
1101	42-43	0.4965459251534696
1101	44-45	0.5097743070318188
1101	46-47	0.4524183872931147
1101	48-49	0.438475977250981
1101	50-51	0.42053711815348294
1101	52-53	0.4453278504299618
1101	54-55	0.3949411451369542
1101	56-57	0.39393054585408294
1101	58-59	0.4035905966012976
1101	60-61	0.3978633588953713
1101	62-63	0.3550962611474304
1101	64-65	0.44852685545639304
1101	66-67	0.36812294644818166
1101	68-69	0.34664956013354953
1101	70-71	0.29602735428057514
1101	72-73	0.33428662535172293
1101	74-75	0.32295607676057614
1101	76-77	0.32254414196336256
1101	78-79	0.32063219584705394
1101	80-81	0.34384379620522054
1101	82-83	0.2854120409713232
1101	84-85	0.2939786292914093
1101	86-87	0.2817772739237867
1101	88-89	0.2822824267631354
1101	90-91	0.29811028895198177
1101	92-93	0.20255125596074208
1101	94-95	0.2224413882650218
1101	96-97	0.21915926013211617
1101	98-99	0.29295367839985786
1101	100	0.28097975534758746
1102	1	0.1897807223358825
1102	2	0.30277441316474807
1102	3	0.3076724264266595
1102	4	0.3498248969411719
1102	5	0.2859435699915096
1102	6	0.3387925262958902
1102	7	0.3304681358143853
1102	8	0.37863948913425816
1102	9	0.4500794834088282
1102	10-11	0.4761599869068007
1102	12-13	0.47911356059298527
1102	14-15	0.546221275921738
1102	16-17	0.4415514160768552
1102	18-19	0.5383611215810191
1102	20-21	0.5284510325078458
1102	22-23	0.49023198367085286
1102	24-25	0.48170783677311846
1102	26-27	0.42068131015558663
1102	28-29	0.49164921910676895
1102	30-31	0.4439407655297529
1102	32-33	0.4999957547820202
1102	34-35	0.5024530064132904
1102	36-37	0.5397407459080554
1102	38-39	0.54672453385529
1102	40-41	0.5303781442338931
1102	42-43	0.5168178824863432
1102	44-45	0.4391821228318591
1102	46-47	0.4582911693945064
1102	48-49	0.4024337950384904
1102	50-51	0.4301469228728152
1102	52-53	0.41755186079434026
1102	54-55	0.3405796854452703
1102	56-57	0.3518359959982007
1102	58-59	0.393730697102356
1102	60-61	0.4104414539031467
1102	62-63	0.36429600263513606
1102	64-65	0.37217972379662
1102	66-67	0.4711637261774442
1102	68-69	0.4348300573352333
1102	70-71	0.36950082003952645
1102	72-73	0.39378286825626674
1102	74-75	0.38914851755999535
1102	76-77	0.3413827571310222
1102	78-79	0.36240756177810596
1102	80-81	0.3622490916517407
1102	82-83	0.3574351762972299
1102	84-85	0.3505839538240565
1102	86-87	0.38456126132150814
1102	88-89	0.3212869877167499
1102	90-91	0.30566424245546386
1102	92-93	0.29594409983917913
1102	94-95	0.2804126369031046
1102	96-97	0.3410703263914989
1102	98-99	0.3696245647003842
1102	100	0.39059239232557275
1103	1	0.26079217828082335
1103	2	0.34216513046453656
1103	3	0.38577965217249144
1103	4	0.3270145007270031
1103	5	0.30679013501973884
1103	6	0.3634856370358506
1103	7	0.39339918564324705
1103	8	0.39836545748726593
1103	9	0.4847962019375487
1103	10-11	0.48853816199278555
1103	12-13	0.47266495655073726
1103	14-15	0.5830509414143137
1103	16-17	0.5470843739968103
1103	18-19	0.48835879223879175
1103	20-21	0.515264815547738
1103	22-23	0.5090381459109921
1103	24-25	0.5131369759732181
1103	26-27	0.5834270163067288
1103	28-29	0.5268556865727945
1103	30-31	0.4888567709641265
1103	32-33	0.5444042161144935
1103	34-35	0.5310435927649095
1103	36-37	0.5345048075284424
1103	38-39	0.4709010356276764
1103	40-41	0.5932719182745672
1103	42-43	0.5745389575794135
1103	44-45	0.5120133126863848
1103	46-47	0.4436688024823141
1103	48-49	0.439259130100794
1103	50-51	0.3986342556067868
1103	52-53	0.37241484560210125
1103	54-55	0.323379765640297
1103	56-57	0.2889489069803304
1103	58-59	0.2795511514036306
1103	60-61	0.32009300450649647
1103	62-63	0.32513763248788763
1103	64-65	0.3575875373320798
1103	66-67	0.32766898563019
1103	68-69	0.2599216686605601
1103	70-71	0.27389527249322754
1103	72-73	0.2869895215646707
1103	74-75	0.2730446826799309
1103	76-77	0.23922221330978743
1103	78-79	0.2506282801472537
1103	80-81	0.20593319067794624
1103	82-83	0.19400742737987287
1103	84-85	0.15848587172490625
1103	86-87	0.22870170310871885
1103	88-89	0.23873867848463703
1103	90-91	0.2562588370432337
1103	92-93	0.20247022237066403
1103	94-95	0.222480525929452
1103	96-97	0.23494040035348007
1103	98-99	0.2641085623717956
1103	100	0.2648484641758664
1104	1	0.13804608672539587
1104	2	0.13462191639338528
1104	3	0.19423358279789227
1104	4	0.2340575586599556
1104	5	0.1827205077783347
1104	6	0.2082494261664536
1104	7	0.217341643606332
1104	8	0.2984387880663206
1104	9	0.3368582360699719
1104	10-11	0.2862161066618967
1104	12-13	0.31525336345944766
1104	14-15	0.3334956275585981
1104	16-17	0.29879826116643926
1104	18-19	0.28509654949187535
1104	20-21	0.3354394549031383
1104	22-23	0.296966780534099
1104	24-25	0.35766473529020715
1104	26-27	0.33116478706429575
1104	28-29	0.32285692922494746
1104	30-31	0.32154884814240603
1104	32-33	0.30618166760678633
1104	34-35	0.3363867699556593
1104	36-37	0.29886802837096127
1104	38-39	0.36551641279674385
1104	40-41	0.35899248383307736
1104	42-43	0.3513384578415497
1104	44-45	0.2685378698854848
1104	46-47	0.24734216136105402
1104	48-49	0.20144185972165474
1104	50-51	0.19364907887662497
1104	52-53	0.2493688527728679
1104	54-55	0.1758459246416919
1104	56-57	0.18968569047598294
1104	58-59	0.2310766987328634
1104	60-61	0.2131547736879753
1104	62-63	0.19586977426304486
1104	64-65	0.22879478023256894
1104	66-67	0.23842677040135385
1104	68-69	0.22838367568839146
1104	70-71	0.23671621477922145
1104	72-73	0.27153180358695295
1104	74-75	0.2423469080407159
1104	76-77	0.15693782236171927
1104	78-79	0.1773692928191224
1104	80-81	0.2204930213674885
1104	82-83	0.23094345206655476
1104	84-85	0.13037081896239044
1104	86-87	0.11580455605783868
1104	88-89	0.07546706266516523
1104	90-91	0.13137004293807308
1104	92-93	0.07711854093609816
1104	94-95	0.14223289300971587
1104	96-97	0.1390788873509834
1104	98-99	0.14559913426546345
1104	100	0.15389456551906378
1105	1	0.23273857554868016
1105	2	0.23879687189474197
1105	3	0.24398044995803403
1105	4	0.22614827798806658
1105	5	0.2193416694602348
1105	6	0.2662551873108825
1105	7	0.27491231802668636
1105	8	0.2684439645626071
1105	9	0.3718350521176248
1105	10-11	0.4047565357163734
1105	12-13	0.3999067113039274
1105	14-15	0.4105685308874527
1105	16-17	0.37127214808984377
1105	18-19	0.3923750948230875
1105	20-21	0.37095689198505255
1105	22-23	0.35667388095638586
1105	24-25	0.3597072235342367
1105	26-27	0.3896538345242817
1105	28-29	0.33521107742596
1105	30-31	0.327763423446477
1105	32-33	0.3389151192499611
1105	34-35	0.3002068343490407
1105	36-37	0.29162643624937346
1105	38-39	0.3345551530919195
1105	40-41	0.39963781400323484
1105	42-43	0.3678203817668759
1105	44-45	0.3738064598494404
1105	46-47	0.26252277590032946
1105	48-49	0.2942648365209237
1105	50-51	0.2890026223401705
1105	52-53	0.25085483725370494
1105	54-55	0.18855277288444228
1105	56-57	0.22342423917906018
1105	58-59	0.20517590157562893
1105	60-61	0.22892875652055977
1105	62-63	0.2583344722830816
1105	64-65	0.27732930175319837
1105	66-67	0.24109487558622078
1105	68-69	0.24936280511178666
1105	70-71	0.25481881372915893
1105	72-73	0.24343447163187903
1105	74-75	0.29817989595028394
1105	76-77	0.27519189047487913
1105	78-79	0.24868897034811255
1105	80-81	0.27647957264118617
1105	82-83	0.25094637917278106
1105	84-85	0.22394670191734178
1105	86-87	0.18928655287089668
1105	88-89	0.1874224577320156
1105	90-91	0.2119759211826704
1105	92-93	0.187695486806021
1105	94-95	0.14396657757305675
1105	96-97	0.18572934999600577
1105	98-99	0.20731320564817324
1105	100	0.19760244850398223
1106	1	0.21463036776400557
1106	2	0.1899375515836006
1106	3	0.259597006121453
1106	4	0.2583610148668214
1106	5	0.24772940740360383
1106	6	0.2820646980106929
1106	7	0.3295352745076485
1106	8	0.26355058917496166
1106	9	0.3163492172355973
1106	10-11	0.3702428369514692
1106	12-13	0.33386134931589595
1106	14-15	0.4108259995950547
1106	16-17	0.37873618182048574
1106	18-19	0.37289286040890346
1106	20-21	0.3821510752088102
1106	22-23	0.4024954058825827
1106	24-25	0.4469898776052119
1106	26-27	0.43098858122428396
1106	28-29	0.334913838590019
1106	30-31	0.34810170374823457
1106	32-33	0.3694170437485198
1106	34-35	0.2955221201124658
1106	36-37	0.3456889306316029
1106	38-39	0.3197940820599925
1106	40-41	0.3985314313544066
1106	42-43	0.37426807503247517
1106	44-45	0.33263967320819887
1106	46-47	0.38179207870640397
1106	48-49	0.2795049033338657
1106	50-51	0.28819495935888284
1106	52-53	0.31644486529879856
1106	54-55	0.2915109496157484
1106	56-57	0.28940809221297314
1106	58-59	0.3244036104350343
1106	60-61	0.2910980363269289
1106	62-63	0.3397972403600136
1106	64-65	0.33855524282630967
1106	66-67	0.3738824614960947
1106	68-69	0.38158562253395445
1106	70-71	0.400093381523142
1106	72-73	0.36451829947313996
1106	74-75	0.39609550895353607
1106	76-77	0.3350238028032919
1106	78-79	0.26675213254183916
1106	80-81	0.3181072191227301
1106	82-83	0.2933358808519273
1106	84-85	0.3157903608989301
1106	86-87	0.3038146738468299
1106	88-89	0.25615725491312347
1106	90-91	0.2777625241796713
1106	92-93	0.2012483952371511
1106	94-95	0.2128307515275658
1106	96-97	0.33075340681325116
1106	98-99	0.3571625087105659
1106	100	0.3268786560140917
1107	1	0.14858232781598701
1107	2	0.19922240255170465
1107	3	0.23083711520639838
1107	4	0.2369866941083174
1107	5	0.18131306652953327
1107	6	0.22971551303981386
1107	7	0.18942391324578978
1107	8	0.19138038713533234
1107	9	0.24629537251265532
1107	10-11	0.24827525220318591
1107	12-13	0.23357219223868242
1107	14-15	0.33630658329370533
1107	16-17	0.2823593969700937
1107	18-19	0.2799881646207396
1107	20-21	0.2854914454571542
1107	22-23	0.2352008325315964
1107	24-25	0.23351934681564757
1107	26-27	0.31141026073048295
1107	28-29	0.3184235979735135
1107	30-31	0.3062740969937394
1107	32-33	0.32446221618609883
1107	34-35	0.3155440880330147
1107	36-37	0.307770474111571
1107	38-39	0.3104013518832076
1107	40-41	0.3893413990187895
1107	42-43	0.3201961127116988
1107	44-45	0.3115962134060126
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2216	96-97	-0.8984037361072303
2216	98-99	-1.0259530218286699
2216	100	-1.0997617836361577
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	84426.0
3	16952.0
4	10399.0
5	8532.0
6	7200.0
7	7883.0
8	8870.0
9	10255.0
10	11705.0
11	13225.0
12	15181.0
13	17231.0
14	20322.0
15	20956.0
16	20825.0
17	22541.0
18	21334.0
19	18410.0
20	17831.0
21	18932.0
22	21767.0
23	27243.0
24	32730.0
25	40323.0
26	48524.0
27	54487.0
28	65911.0
29	84493.0
30	100784.0
31	115567.0
32	146595.0
33	196442.0
34	270129.0
35	392844.0
36	661901.0
37	1222431.0
38	1846541.0
39	881442.0
40	3145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.2402919849996	22.41133781886476	15.377365084574757	21.971005111560878
2	14.696535980950712	21.317593972038022	41.59551604956568	22.39035399744558
3	17.54305853932545	27.245445556928505	31.32043960035613	23.89105630338991
4	11.76434859614747	18.39249986941189	41.257429855964354	28.585721678476283
5	11.783246976891864	39.353482984088686	36.57195169708135	12.291318341938103
6	27.858050005891478	40.50643021392745	18.857177824234743	12.778341955946324
7	24.628226085192278	34.08848343699449	24.20793218926407	17.075358288549165
8	23.15825357044429	37.65768529070337	21.620686749174155	17.563374389678188
9	23.912017202247736	17.914432453222922	23.532434378924727	34.64111596560461
10-11	22.23643884121361	28.531987468656077	30.273656586449405	18.95791710368091
12-13	22.805977882433297	26.48254064331231	29.957814428891222	20.753667045363176
14-15	20.408970681776733	28.897208179444007	27.476957029630118	23.216864109149146
16-17	19.290501641692963	32.075783994281316	28.406781488369585	20.226932875656136
18-19	19.187305668286996	30.909935161139018	30.552282054283886	19.3504771162901
20-21	20.807861667786817	29.983393623066224	29.83192269227393	19.376822016873028
22-23	20.305183371966862	30.03128139053374	30.01232274195055	19.651212495548855
24-25	19.936757036210608	30.230287027569315	29.935802628864543	19.897153307355534
26-27	19.599308770314476	30.268090648812073	30.093620595620006	20.038979985253444
28-29	19.620307224567394	30.220331505152565	30.416276890387433	19.743084379892608
30-31	20.124867448010047	30.720257542812764	29.647724319496856	19.50715068968033
32-33	19.53287153226869	30.43018716647578	30.17706086032878	19.859880440926755
34-35	20.003677665802826	30.072138128390012	30.09779979315787	19.826384412649286
36-37	20.135924320302475	30.335957726268475	29.666851411781675	19.86126654164737
38-39	19.871761433289407	30.490573831329343	29.89597178736346	19.741692948017782
40-41	19.94124739832088	29.983559870717247	30.004841763470935	20.070350967490942
42-43	19.758145152387886	30.13096352156717	29.97897574557828	20.131915580466668
44-45	19.974040734209556	30.237070025931928	29.720808050865216	20.068081188993293
46-47	20.019642919513384	30.361340122428466	29.637224813099937	19.981792144958217
48-49	19.754642033174274	30.177670784945796	29.71512462467194	20.35256255720799
50-51	19.853873580823855	30.14987702713147	29.555853613315964	20.440395778728707
52-53	19.906145254450543	30.205947763013917	29.53133213941565	20.356574843119883
54-55	20.00884059579724	30.47982933489474	29.45782335785022	20.053506711457807
56-57	19.99852389692427	30.184386838627614	29.3844209773078	20.432668287140316
58-59	20.10329846323152	29.84201107838591	29.662293251999117	20.392397206383446
60-61	20.052877178086035	30.23559072602564	29.35680980432529	20.354722291563036
62-63	19.836382775561376	30.25348475866057	29.488540143059154	20.4215923227189
64-65	20.114771632956145	29.98680565880509	29.422302597818167	20.4761201104206
66-67	19.742928627738127	30.050406136536555	29.427362023175935	20.77930321254938
68-69	20.21385783851321	30.413959397183692	29.380355443198514	19.991827321104587
70-71	20.22845698778454	30.116178508665453	29.198228797329147	20.457135706220857
72-73	20.006233933479145	30.185093154024855	29.467879486903993	20.340793425592004
74-75	20.118015298313157	30.14647582829163	29.271589022718462	20.463919850676753
76-77	20.098918207379292	30.168033706048142	28.979129296865924	20.75391878970664
78-79	20.200274025146356	30.109306362269866	29.422679460580753	20.26774015200302
80-81	20.02655293274371	30.151041160462633	29.33790789921275	20.48449800758091
82-83	19.976798876676288	30.262182260092203	29.51378317404548	20.247235689186034
84-85	19.92398020414936	29.935639873015376	29.399774945689572	20.740604977145697
86-87	20.269084867417654	30.12897993620971	29.130904183740697	20.47103101263194
88-89	20.120644859590982	29.979385078398735	29.253553968901997	20.646416093108286
90-91	19.93630171366719	29.851857864153803	29.322987607101826	20.88885281507718
92-93	20.15663999995141	30.10093174067963	29.115400582288576	20.62702767708038
94-95	20.07284009793711	29.800281583992337	29.324885560472186	20.801992757598367
96-97	20.109998233207097	30.062873379259823	29.102624852922272	20.724503534610804
98-99	20.122328890867614	29.950443444317283	29.079749216738865	20.847478448076238
100	20.258185127221118	29.88986787819947	28.936318758933023	20.915628235646388
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8064.0
1	5639.5
2	3828.5
3	4481.5
4	4206.5
5	3445.0
6	2841.5
7	2666.5
8	2696.0
9	2690.5
10	2311.0
11	1927.5
12	1855.5
13	1867.5
14	2001.0
15	2261.5
16	2698.0
17	3399.0
18	4608.0
19	6383.5
20	8982.5
21	13237.5
22	20127.0
23	29693.5
24	41297.0
25	55962.5
26	75472.5
27	98642.5
28	126353.5
29	152484.5
30	171897.5
31	191441.0
32	210887.0
33	227496.0
34	242657.0
35	254641.0
36	260314.0
37	263270.0
38	265613.5
39	262141.5
40	257622.5
41	249328.0
42	235859.0
43	225705.0
44	214500.5
45	204912.0
46	199569.5
47	192792.0
48	188187.5
49	184593.0
50	177819.5
51	163883.5
52	147565.5
53	132490.5
54	122840.5
55	120132.0
56	105412.5
57	88194.5
58	75793.5
59	63954.5
60	51217.0
61	37115.0
62	27568.5
63	20722.0
64	15694.5
65	12116.0
66	9212.5
67	6461.0
68	4529.0
69	3316.5
70	2299.0
71	1557.0
72	1181.5
73	885.5
74	674.5
75	518.0
76	384.5
77	270.5
78	199.0
79	153.0
80	118.0
81	97.0
82	70.0
83	50.5
84	41.0
85	37.0
86	28.5
87	21.0
88	15.0
89	12.0
90	13.0
91	12.0
92	8.0
93	8.0
94	9.0
95	7.0
96	8.5
97	15.0
98	14.0
99	9.5
100	19.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07817732207826872
2	0.013148487263503733
3	0.01739973025863196
4	0.010886218669667639
5	0.009413466632069645
6	0.007971080615865427
7	0.021149933900762932
8	0.009777858888794923
9	0.009990421038551335
10-11	0.013695075648591647
12-13	0.01519060220223497
14-15	0.010294081252489064
16-17	0.013383823929305472
18-19	0.009079440396738142
20-21	0.013467330488138349
22-23	0.014029101883923152
24-25	0.016253412951017027
26-27	0.005541798904363583
28-29	0.012154000062857663
30-31	0.010407953832715713
32-33	0.013080163715367741
34-35	0.00926163652510078
36-37	0.01000560404924822
38-39	0.01998843358245111
40-41	0.013641935111152545
42-43	0.00883651222558796
44-45	0.020907005729612748
46-47	0.018811750253442405
48-49	0.01877379272670019
50-51	0.010779937594789433
52-53	0.010734388562698774
54-55	0.01184274834357149
56-57	0.021301764007731795
58-59	0.0022926346152298653
60-61	0.007303028145202418
62-63	0.016109174349396606
64-65	0.025325261842406726
66-67	0.013672301132546315
68-69	0.014469409194132862
70-71	0.02647157915002166
72-73	0.020975329277748735
74-75	0.021256214975641136
76-77	0.010111885124126425
78-79	0.011873114364965263
80-81	0.020481881430099925
82-83	0.012935925113747321
84-85	0.01195662092379814
86-87	0.017270674667708423
88-89	0.015646092523141565
90-91	0.015873837683594863
92-93	0.008669499107922207
94-95	0.016131948865441934
96-97	0.014127791453452911
98-99	0.01065847350921434
100	0.007470041262868171
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6586309.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.360606544245
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.19437515639899	35.538650774826905
2	16.675934992779816	17.129722718431424
3	6.3368745434029465	9.76397160431883
4	2.9918869268927706	6.146605091080397
5	1.605518650033256	4.123020584190272
6	0.9185701138024747	2.830699091898676
7	0.5712512530934302	2.0537867593627013
8	0.36864208180219626	1.514694473527519
9	0.25744157675251855	1.190011997854454
>10	0.9580797361568671	8.321347135293175
>50	0.06351458156497264	2.2459588335867466
>100	0.0502795133171991	5.302833145266958
>500	0.005501376736580415	1.9515248252987487
>1k	0.0020999209150833692	1.7389431569560854
>5k	2.9576350801499705E-5	0.14822980810703562
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9758	0.1481558183802187	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.5183010696886526E-5	0.0
5	0.0	0.0	0.0	1.5183010696886526E-5	0.0
6	0.0	0.0	0.0	1.5183010696886526E-5	0.0
7	0.0	0.0	0.0	1.5183010696886526E-5	0.0
8	0.0	0.0	0.0	1.5183010696886526E-5	0.0
9	0.0	0.0	0.0	1.5183010696886526E-5	1.5183010696886526E-5
10-11	0.0	0.0	0.0	3.7957526742216315E-5	1.5183010696886526E-5
12-13	0.0	0.0	0.0	6.832354813598936E-5	1.5183010696886526E-5
14-15	0.0	0.0	0.0	8.35065588328759E-5	1.5183010696886526E-5
16-17	0.0	0.0	0.0	9.109806418131916E-5	1.5183010696886526E-5
18-19	0.0	0.0	0.0	1.2146408557509221E-4	1.5183010696886526E-5
20-21	0.0	0.0	0.0	1.7460462301419505E-4	1.5183010696886526E-5
22-23	0.0	0.0	0.0	3.5680075137683336E-4	1.5183010696886526E-5
24-25	0.0	0.0	0.0	8.578401043740887E-4	1.5183010696886526E-5
26-27	0.0	0.0	0.0	0.0016245821445668583	1.5183010696886526E-5
28-29	0.0	0.0	0.0	0.003841301706312291	1.5183010696886526E-5
30-31	0.0	0.0	0.0	0.011182287378256927	1.5183010696886526E-5
32-33	0.0	0.0	0.0	0.027458474845319283	1.5183010696886526E-5
34-35	0.0	0.0	0.0	0.05577478979501266	1.5183010696886526E-5
36-37	0.0	0.0	0.0	0.09976756328924136	1.5183010696886526E-5
38-39	0.0	0.0	0.0	0.15570177469657132	1.5183010696886526E-5
40-41	0.0	0.0	0.0	0.21290376749709133	1.5183010696886526E-5
42-43	0.0	0.0	0.0	0.27164683588334526	2.277451604532979E-5
44-45	0.0	0.0	0.0	0.34888281129840704	3.0366021393773052E-5
46-47	0.0	0.0	0.0	0.4451127330952738	3.0366021393773052E-5
48-49	0.0	0.0	0.0	0.5446981002561526	3.0366021393773052E-5
50-51	0.0	0.0	0.0	0.6455208827888276	3.0366021393773052E-5
52-53	0.0	0.0	0.0	0.7450227433908734	3.0366021393773052E-5
54-55	0.0	0.0	0.0	0.8605578632888314	3.0366021393773052E-5
56-57	0.0	0.0	0.0	1.0051532656606303	3.0366021393773052E-5
58-59	0.0	0.0	0.0	1.1657363782962507	3.0366021393773052E-5
60-61	0.0	0.0	0.0	1.3332809013363933	3.0366021393773052E-5
62-63	0.0	0.0	0.0	1.5034748597431429	3.0366021393773052E-5
64-65	0.0	0.0	0.0	1.6866806583171243	3.0366021393773052E-5
66-67	0.0	0.0	0.0	1.8966541047497165	3.0366021393773052E-5
68-69	0.0	0.0	0.0	2.1375401609611697	3.0366021393773052E-5
70-71	0.0	0.0	0.0	2.398536114840649	3.0366021393773052E-5
72-73	0.0	0.0	0.0	2.6670324760043904	3.0366021393773052E-5
74-75	0.0	0.0	0.0	2.9407138353211186	3.0366021393773052E-5
76-77	0.0	0.0	0.0	3.2343684452096007	3.0366021393773052E-5
78-79	0.0	0.0	0.0	3.57017109279264	3.0366021393773052E-5
80-81	0.0	0.0	0.0	3.9378201053123987	3.0366021393773052E-5
82-83	0.0	2.277451604532979E-5	0.0	4.3139867868331105	3.0366021393773052E-5
84-85	0.0	3.0366021393773052E-5	0.0	4.694002361565484	3.0366021393773052E-5
86-87	0.0	3.0366021393773052E-5	0.0	5.096420468581113	3.0366021393773052E-5
88	0.0	3.0366021393773052E-5	0.0	5.4212306164196065	3.0366021393773052E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7685	0.0	50.73105	1
GTATCAA	14175	0.0	35.599167	1
ATCAACG	15705	0.0	30.827017	3
TCAACGC	15815	0.0	30.492096	4
TATCAAC	17625	0.0	27.842201	2
CAACGCA	18275	0.0	26.43979	5
TGGTATC	3765	0.0	25.84268	2
GTGGTAT	4160	0.0	25.097057	1
AACGCAG	19820	0.0	24.472698	6
CGCAGAG	19365	0.0	24.413511	8
ACGCAGA	20450	0.0	23.397005	7
GTACATG	22430	0.0	23.378075	1
TACATGG	21620	0.0	22.740929	2
ACATGGG	22190	0.0	21.097658	3
GCAGAGT	21930	0.0	20.91626	9
ATACCGT	610	0.0	18.492144	6
TACCGCG	485	0.0	18.41033	8
AGAGTAC	20890	0.0	18.189304	10-11
CAGAGTA	22310	0.0	18.0006	10-11
GGTACCG	505	0.0	17.683493	6
>>END_MODULE