FastQCFastQC Report
Wed 25 May 2016
SRR2049453_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049453_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6586309
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289610.4397151727925307No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279480.4243347829565846No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161460.24514489071192988No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86770.1317429838168844No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA152550.063.7154731
GTATCAA292650.041.5766141
TGGTATC50050.039.820032
GTGGTAT55600.037.5031
ATCAACG365550.032.8536763
TCAACGC369950.032.577284
TATCAAC378300.031.907922
CAACGCA392450.030.7095555
AACGCAG412200.029.303016
CGCAGAG438550.027.1887028
ACGCAGA449350.026.6711987
GCAGAGT470900.025.1013099
GAGTACT360700.022.63292912-13
GTACATG221350.022.1789951
CAGAGTA476500.022.15800310-11
TACATGG216800.021.616022
AGAGTAC464000.020.94689410-11
GTACTTT395400.020.55753314-15
ACATGGG219850.020.3967883
AGTACTT373550.020.03002212-13
Produced by FastQC (version 0.11.5)