##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049453_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6586309
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.976456312632767	33.0	31.0	34.0	30.0	34.0
2	32.14859141895711	33.0	31.0	34.0	30.0	34.0
3	32.09666628152429	34.0	31.0	34.0	30.0	34.0
4	35.83073630465865	37.0	35.0	37.0	35.0	37.0
5	35.88842870870468	37.0	35.0	37.0	35.0	37.0
6	35.955733628653014	37.0	35.0	37.0	35.0	37.0
7	35.945751558270345	37.0	35.0	37.0	35.0	37.0
8	35.95969897555672	37.0	35.0	37.0	35.0	37.0
9	37.67479797865542	39.0	37.0	39.0	35.0	39.0
10-11	37.64874036429205	39.0	37.0	39.0	35.0	39.0
12-13	37.62432790505274	39.0	37.0	39.0	35.0	39.0
14-15	38.90526256815464	40.0	38.0	41.0	35.0	41.0
16-17	38.920185266133124	40.0	38.0	41.0	35.5	41.0
18-19	39.02295663625864	40.0	38.5	41.0	36.0	41.0
20-21	39.057975111705204	40.0	39.0	41.0	35.5	41.0
22-23	39.02355674475643	40.0	39.0	41.0	35.0	41.0
24-25	38.99457442704252	40.0	39.0	41.0	35.0	41.0
26-27	38.886405496614266	40.0	38.0	41.0	35.0	41.0
28-29	38.80253325496875	40.0	38.0	41.0	35.0	41.0
30-31	38.65934926527134	40.0	38.0	41.0	35.0	41.0
32-33	38.48636527681893	40.0	38.0	41.0	34.5	41.0
34-35	38.34988359944849	40.0	38.0	41.0	34.0	41.0
36-37	38.19186474548947	40.0	38.0	41.0	34.0	41.0
38-39	38.06418245788346	40.0	38.0	41.0	34.0	41.0
40-41	37.951374206706674	40.0	38.0	41.0	33.5	41.0
42-43	37.78271608574696	40.0	38.0	41.0	33.0	41.0
44-45	37.564346130738784	40.0	37.5	41.0	33.0	41.0
46-47	37.25931018420181	40.0	37.0	41.0	32.5	41.0
48-49	36.96273314841439	40.0	37.0	41.0	31.0	41.0
50-51	36.788338961928446	40.0	37.0	41.0	31.0	41.0
52-53	36.66904931123031	40.0	37.0	41.0	31.0	41.0
54-55	36.53764399757132	40.0	36.0	41.0	31.0	41.0
56-57	36.400951731842525	40.0	36.0	41.0	30.0	41.0
58-59	36.23538775663273	39.5	35.5	41.0	30.0	41.0
60-61	36.06845708271507	39.0	35.0	41.0	30.0	41.0
62-63	35.87800830480319	39.0	35.0	41.0	29.5	41.0
64-65	35.682230593796916	39.0	35.0	41.0	29.0	41.0
66-67	35.44480884513618	38.5	35.0	40.5	29.0	41.0
68-69	35.17791459526117	38.0	35.0	40.0	29.0	41.0
70-71	34.91373400488801	37.0	35.0	40.0	28.5	41.0
72-73	34.598388110852376	37.0	34.5	39.5	28.0	41.0
74-75	34.285922965351304	36.0	34.0	39.0	28.0	41.0
76-77	33.9683915528409	36.0	34.0	39.0	28.0	40.5
78-79	33.65079530887482	35.5	34.0	38.5	27.5	40.0
80-81	33.324818574409434	35.0	34.0	37.0	27.0	39.0
82-83	33.03179512227562	35.0	34.0	37.0	27.0	39.0
84-85	32.71859937029981	35.0	34.0	36.5	26.0	39.0
86-87	32.44708986474822	35.0	34.0	36.0	26.0	37.0
88-89	32.201052213007316	35.0	33.5	36.0	26.0	37.0
90-91	31.979788679820516	35.0	33.0	35.5	26.0	37.0
92-93	31.794275215450718	35.0	33.0	35.0	25.5	36.0
94-95	31.614095087248412	35.0	33.0	35.0	25.0	36.0
96-97	31.459071158064404	35.0	33.0	35.0	25.0	36.0
98-99	31.315163621992227	35.0	33.0	35.0	24.5	36.0
100	31.19820524667154	35.0	33.0	35.0	24.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3009202095221113
1101	2	0.30369484296865323
1101	3	0.4446278563857504
1101	4	0.27393364914033924
1101	5	0.2621225151562214
1101	6	0.21197924866869755
1101	7	0.20225476374375262
1101	8	0.2446853974965819
1101	9	0.30584142763347444
1101	10-11	0.27351913448741527
1101	12-13	0.252348608050184
1101	14-15	0.388890354468586
1101	16-17	0.37124417182587166
1101	18-19	0.33446803124906666
1101	20-21	0.3815033947581483
1101	22-23	0.3658360377986014
1101	24-25	0.3872403157085884
1101	26-27	0.3439512173308401
1101	28-29	0.39211263176216704
1101	30-31	0.3616341686875799
1101	32-33	0.38202975647681114
1101	34-35	0.3455576015022217
1101	36-37	0.36125775103899116
1101	38-39	0.38773271064962245
1101	40-41	0.3218757715052263
1101	42-43	0.3129513924041518
1101	44-45	0.31376189588135617
1101	46-47	0.3452993126432773
1101	48-49	0.3315192239643494
1101	50-51	0.32081151257024487
1101	52-53	0.28170381586911475
1101	54-55	0.30901704254101503
1101	56-57	0.3382622626911811
1101	58-59	0.30871878596443736
1101	60-61	0.2323811946253116
1101	62-63	0.2941663098447762
1101	64-65	0.2945278887446534
1101	66-67	0.3193969964487522
1101	68-69	0.26048778068912526
1101	70-71	0.31182657420661997
1101	72-73	0.32471392518017694
1101	74-75	0.34374640484987395
1101	76-77	0.2930961384900854
1101	78-79	0.29425500061196175
1101	80-81	0.2932616898593494
1101	82-83	0.24487731744728336
1101	84-85	0.2094321127077734
1101	86-87	0.19834995520978538
1101	88-89	0.2724277573389742
1101	90-91	0.28267419413695194
1101	92-93	0.19925540527498597
1101	94-95	0.16708448599289483
1101	96-97	0.1414783363762524
1101	98-99	0.18185501768773804
1101	100	0.1666974897685023
1102	1	0.31128868042780766
1102	2	0.32928003298249564
1102	3	0.3978380319988588
1102	4	0.22831476026843234
1102	5	0.22780487991148135
1102	6	0.18858072349605237
1102	7	0.2501616922370502
1102	8	0.2325556025332176
1102	9	0.21754317505119047
1102	10-11	0.25687365053506284
1102	12-13	0.27471860985509267
1102	14-15	0.35062126265755467
1102	16-17	0.3437556220967224
1102	18-19	0.37670091791733284
1102	20-21	0.3605660680052907
1102	22-23	0.3195305507769959
1102	24-25	0.32456889817463264
1102	26-27	0.34464088684311633
1102	28-29	0.34591509157615974
1102	30-31	0.3592351637086608
1102	32-33	0.40896451641600606
1102	34-35	0.4203903664705564
1102	36-37	0.42319729259433103
1102	38-39	0.4128829098568829
1102	40-41	0.4277418217082598
1102	42-43	0.3865201760842041
1102	44-45	0.43145512927065965
1102	46-47	0.40305432414595543
1102	48-49	0.40860611146413817
1102	50-51	0.3315438573774969
1102	52-53	0.32406994683245927
1102	54-55	0.32799406057186786
1102	56-57	0.31953040544457423
1102	58-59	0.30623763359554346
1102	60-61	0.2965932406520011
1102	62-63	0.3169221210245965
1102	64-65	0.2933178775716314
1102	66-67	0.26486817061455525
1102	68-69	0.2721723601808179
1102	70-71	0.28082484144293574
1102	72-73	0.26616211635963793
1102	74-75	0.23279216367398448
1102	76-77	0.23812913820034254
1102	78-79	0.21555772836106968
1102	80-81	0.21942983241591207
1102	82-83	0.20547821520193565
1102	84-85	0.135425009260409
1102	86-87	0.1293756393174874
1102	88-89	0.11296810129802992
1102	90-91	0.16395612962008244
1102	92-93	0.1521386624136447
1102	94-95	0.08332163856580621
1102	96-97	0.09784340902071875
1102	98-99	0.17717716075481604
1102	100	0.20370373636515282
1103	1	0.29153220968554194
1103	2	0.2804082741927658
1103	3	0.3854421311866716
1103	4	0.21765591749827706
1103	5	0.23237541548722618
1103	6	0.22655263781514634
1103	7	0.22088207509253976
1103	8	0.18487926974861324
1103	9	0.24895910833276957
1103	10-11	0.25654271981196786
1103	12-13	0.21608255777326946
1103	14-15	0.29922361944687736
1103	16-17	0.2905788096191273
1103	18-19	0.2817096475849823
1103	20-21	0.27144607834782164
1103	22-23	0.2579900993189099
1103	24-25	0.22588606229578545
1103	26-27	0.2603290635438569
1103	28-29	0.2604444804893262
1103	30-31	0.31441640357775924
1103	32-33	0.3140769302193007
1103	34-35	0.31281748551862165
1103	36-37	0.3112760599644986
1103	38-39	0.30337863807049104
1103	40-41	0.26442276280291566
1103	42-43	0.29844962138133724
1103	44-45	0.29110333420631207
1103	46-47	0.301598485255127
1103	48-49	0.32859602774796315
1103	50-51	0.32440990277682147
1103	52-53	0.29863890173803753
1103	54-55	0.2642833881065414
1103	56-57	0.2759503061830344
1103	58-59	0.2388584996285701
1103	60-61	0.22310616105250602
1103	62-63	0.29118658860702595
1103	64-65	0.25473183122795007
1103	66-67	0.23346790515791582
1103	68-69	0.2601698846392324
1103	70-71	0.20450356467553377
1103	72-73	0.2355797214650508
1103	74-75	0.2696388731340491
1103	76-77	0.2167604343839713
1103	78-79	0.22873967170414033
1103	80-81	0.23261716670277366
1103	82-83	0.19642735713917858
1103	84-85	0.23290357520875915
1103	86-87	0.1531178481727551
1103	88-89	0.11780843025417909
1103	90-91	0.1610833373346665
1103	92-93	0.14937464851637117
1103	94-95	0.1333834639797793
1103	96-97	0.15672512148746875
1103	98-99	0.2356880469893632
1103	100	0.15772008418127115
1104	1	0.2270540393360747
1104	2	0.18554253810899013
1104	3	0.27171791176714066
1104	4	0.19807366517153469
1104	5	0.12943766294279868
1104	6	0.13266958646473626
1104	7	0.17607795175327823
1104	8	0.14348543661607494
1104	9	0.2038856241849487
1104	10-11	0.23967530032771123
1104	12-13	0.22927128828781917
1104	14-15	0.32239576572990813
1104	16-17	0.34593840133894105
1104	18-19	0.2720020102458065
1104	20-21	0.3045747210497112
1104	22-23	0.25921605609713083
1104	24-25	0.22897342188942105
1104	26-27	0.2429070516783085
1104	28-29	0.23077907037765755
1104	30-31	0.24718589255946455
1104	32-33	0.21684403022076992
1104	34-35	0.1981184510179972
1104	36-37	0.18109498147676106
1104	38-39	0.193913687183219
1104	40-41	0.19015393237426537
1104	42-43	0.15841062925612448
1104	44-45	0.16684772742266318
1104	46-47	0.13073273267914942
1104	48-49	0.05613994811592704
1104	50-51	0.0764276529358554
1104	52-53	0.08072747159694416
1104	54-55	0.044287987998963274
1104	56-57	0.013218828159558882
1104	58-59	0.015512760886920773
1104	60-61	-0.040878946004042405
1104	62-63	-0.011669013611388834
1104	64-65	-0.019261250648582973
1104	66-67	0.04590518247572817
1104	68-69	0.04002796080861515
1104	70-71	0.003933525786194991
1104	72-73	0.01094750670588951
1104	74-75	0.020412985192884037
1104	76-77	0.04936997880818694
1104	78-79	0.004794500892053577
1104	80-81	-0.015731622500133824
1104	82-83	-0.005099158211066879
1104	84-85	-0.03340267293661725
1104	86-87	-0.08313358249656488
1104	88-89	-0.061121797779307485
1104	90-91	-0.0820146430030384
1104	92-93	-0.014555669746229682
1104	94-95	0.004877569747666399
1104	96-97	-0.031908584980115506
1104	98-99	-0.017576191553956733
1104	100	-0.029618977844613426
1105	1	0.2392514521748268
1105	2	0.19586315128647414
1105	3	0.2955979301083431
1105	4	0.18618586890904254
1105	5	0.18719774771678033
1105	6	0.14968444586614993
1105	7	0.16555139745060643
1105	8	0.14159619908404153
1105	9	0.21629453767247497
1105	10-11	0.18209139219478487
1105	12-13	0.1700171810306017
1105	14-15	0.21950528187548457
1105	16-17	0.2981317567486954
1105	18-19	0.24995953382571656
1105	20-21	0.2580906177684099
1105	22-23	0.2328619758610131
1105	24-25	0.26197358855007025
1105	26-27	0.22576240752948706
1105	28-29	0.2753025945921834
1105	30-31	0.22778868634853922
1105	32-33	0.25539393729657434
1105	34-35	0.2952139557998379
1105	36-37	0.25445748264025525
1105	38-39	0.3003267374792742
1105	40-41	0.28414355075413056
1105	42-43	0.3116310651507419
1105	44-45	0.27982288009269496
1105	46-47	0.33473660265328675
1105	48-49	0.36344528603052595
1105	50-51	0.28065343771746853
1105	52-53	0.2944262582235311
1105	54-55	0.32614605058775936
1105	56-57	0.30421229080786105
1105	58-59	0.33842457966891715
1105	60-61	0.2753367449565687
1105	62-63	0.2510756172218649
1105	64-65	0.24037544733229765
1105	66-67	0.24316305376917313
1105	68-69	0.2950015941711328
1105	70-71	0.29656879305949246
1105	72-73	0.3245515006130546
1105	74-75	0.31857429384880476
1105	76-77	0.2955335124559326
1105	78-79	0.2333311045622679
1105	80-81	0.24312733906022288
1105	82-83	0.16477463162190986
1105	84-85	0.17293300789134491
1105	86-87	0.20392031533373256
1105	88-89	0.15300680027699798
1105	90-91	0.19343133907981525
1105	92-93	0.22813606648354678
1105	94-95	0.20804642542842444
1105	96-97	0.2209423165443809
1105	98-99	0.23904373725526185
1105	100	0.2149012731861788
1106	1	0.20027743416027022
1106	2	0.21321810576304046
1106	3	0.3030940085994729
1106	4	0.16689211387101466
1106	5	0.183114485215647
1106	6	0.19637629690533487
1106	7	0.1665637091826042
1106	8	0.1526858856831268
1106	9	0.19205496838628733
1106	10-11	0.15779842586291437
1106	12-13	0.15618827716404127
1106	14-15	0.22600778224331464
1106	16-17	0.2229681305723119
1106	18-19	0.24844398433707227
1106	20-21	0.208292205226428
1106	22-23	0.24106566864782053
1106	24-25	0.23589301891944103
1106	26-27	0.23281678443926523
1106	28-29	0.22643704063327874
1106	30-31	0.21217479241548176
1106	32-33	0.19118141474642414
1106	34-35	0.19791305668569947
1106	36-37	0.18430062435430727
1106	38-39	0.21429682884015477
1106	40-41	0.225780721378392
1106	42-43	0.1461129031702626
1106	44-45	0.17870142998224026
1106	46-47	0.09697199288023484
1106	48-49	0.14367734802310395
1106	50-51	0.10916872208281347
1106	52-53	0.08465063437339637
1106	54-55	0.1366567962695555
1106	56-57	0.13678383971863894
1106	58-59	0.09588169650044165
1106	60-61	0.06385947137182768
1106	62-63	0.10090855600612514
1106	64-65	0.1067951374188496
1106	66-67	0.10669998936258906
1106	68-69	0.15797382116341652
1106	70-71	0.14416455460148114
1106	72-73	0.1817661399793309
1106	74-75	0.18328897510845366
1106	76-77	0.11720532408692463
1106	78-79	0.06285244523490263
1106	80-81	0.014948429950322861
1106	82-83	0.03253945034465744
1106	84-85	0.04552589841660648
1106	86-87	0.08426851698951054
1106	88-89	0.061544390103996705
1106	90-91	0.0830549255130748
1106	92-93	0.11914026706929093
1106	94-95	0.15996196114533845
1106	96-97	0.17912870592271446
1106	98-99	0.18371892647805055
1106	100	0.16757840277746539
1107	1	0.1681352672201868
1107	2	0.15078885490952842
1107	3	0.255566127224526
1107	4	0.173245286538652
1107	5	0.1353637319337082
1107	6	0.13301379805525926
1107	7	0.09890768582374676
1107	8	0.11518967753924159
1107	9	0.1490557751187822
1107	10-11	0.1318019896793814
1107	12-13	0.14809537507304782
1107	14-15	0.1690403105785947
1107	16-17	0.12906531803868404
1107	18-19	0.1426795376712704
1107	20-21	0.1728483434535022
1107	22-23	0.19230680835188707
1107	24-25	0.17032951222597603
1107	26-27	0.16519223827205565
1107	28-29	0.17508114464981617
1107	30-31	0.1559592532191303
1107	32-33	0.12600334412022107
1107	34-35	0.09737168455673384
1107	36-37	0.12238498314424362
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2216	72-73	-0.6337600104522636
2216	74-75	-0.5972118109506894
2216	76-77	-0.533397678597737
2216	78-79	-0.5407276123951021
2216	80-81	-0.5511266004785611
2216	82-83	-0.5875714051302197
2216	84-85	-0.6781310134971505
2216	86-87	-0.6066006956033156
2216	88-89	-0.6104407673882335
2216	90-91	-0.578179996225721
2216	92-93	-0.6053035670243254
2216	94-95	-0.6308195675495689
2216	96-97	-0.6434483447446695
2216	98-99	-0.7001799598600442
2216	100	-0.7214222063925604
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	541.0
3	44.0
4	70.0
5	169.0
6	643.0
7	1714.0
8	3753.0
9	6302.0
10	7584.0
11	9353.0
12	13288.0
13	19072.0
14	23357.0
15	31120.0
16	39551.0
17	55945.0
18	56468.0
19	28592.0
20	18361.0
21	16777.0
22	16369.0
23	17643.0
24	20219.0
25	24429.0
26	30738.0
27	39502.0
28	51521.0
29	67002.0
30	87209.0
31	113836.0
32	149445.0
33	194874.0
34	263460.0
35	385748.0
36	628796.0
37	1220252.0
38	1934503.0
39	1003280.0
40	4779.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.13775457458906	22.276409168029872	15.775506108573895	21.81033014880717
2	14.904159522427507	21.53230284215332	41.13820047009638	22.42533716532279
3	17.021491096151124	27.380479719369376	32.132549505345104	23.4654796791344
4	11.671058858611099	18.81451356138924	40.72948900514689	28.784938574852774
5	11.195770949513676	38.85766618660813	36.83786696081641	13.108695903061784
6	27.295478369025627	40.91165960351615	18.297993287881383	13.494868739576843
7	23.752635960444614	34.81573670473098	24.036892286711723	17.394735048112683
8	23.414388848139374	36.95679932417383	21.160152066961935	18.46865976072486
9	23.99486571310274	17.093747044057604	23.554361023753973	35.35702621908568
10-11	22.323945019888985	28.280042737138512	29.818203184818692	19.577809058153818
12-13	23.4603402300135	26.313197573937085	30.038744006696316	20.187718189353095
14-15	20.651004986252545	28.25225782756321	28.568724911023764	22.52801227516049
16-17	18.544081366361645	31.419654012588843	29.518126161405423	20.51813845964409
18-19	18.274886586705847	29.227766264838166	33.36165217878481	19.135694969671178
20-21	19.818672339849225	28.262703738922667	33.1558768348099	18.762747086418205
22-23	19.371532674825914	28.263174412254266	33.37735900335074	18.987933909569076
24-25	19.12270590401999	28.363040665113036	33.30754752016645	19.206705910700514
26-27	18.806200863032693	28.495132858175953	33.374876581101795	19.323789697689556
28-29	18.804986222176943	28.407200451724933	33.71017059782649	19.07764272827163
30-31	19.30793104301666	28.814196236465673	33.0181289702624	18.859743750255266
32-33	18.77076371606616	28.60174795928949	33.49112530250251	19.13636302214184
34-35	19.242461900891684	28.29628096707883	33.28122017961805	19.180036952411434
36-37	19.338911976343653	28.594239960499877	32.74897214813335	19.31787591502312
38-39	19.186565647011093	28.736428855676223	32.89861590156186	19.178389595750822
40-41	19.20302403060652	28.380235424727264	32.874641927671476	19.54209861699474
42-43	19.196624391597783	28.48534740778181	32.693151809306244	19.624876391314164
44-45	19.502668338214924	28.631946967565597	32.249094295454405	19.616290398765074
46-47	19.656031929264177	28.861969579623427	31.727034064147308	19.75496442696509
48-49	19.58057236610065	28.83699352702705	31.354981978525455	20.227452128346847
50-51	19.7476006667771	28.896943644763706	30.971231383161644	20.38422430529755
52-53	19.800968949376653	29.0771735732411	30.839275837195007	20.282581640187242
54-55	19.94151807939773	29.36668322120933	30.723710351275653	19.968088348117284
56-57	19.93742625801492	28.999952780836736	30.64760551015751	20.415015450990836
58-59	19.917612429055485	28.629176068113416	31.0073138080828	20.445897694748304
60-61	19.83967044364302	29.01878730560622	30.66736619857951	20.474176052171252
62-63	19.626128989696657	28.95202001606666	30.8702719535327	20.55157904070398
64-65	19.768780966699254	28.831702247799186	30.84588044684815	20.553636338653412
66-67	19.623069613041235	28.82991065253695	30.780183559562722	20.76683617485909
68-69	20.131692879881584	29.086457984282244	30.580397913307745	20.20145122252843
70-71	20.094737431845363	28.869697732068143	30.43884670458067	20.596718131505824
72-73	19.84761874974284	28.870046941314172	30.65369389744696	20.62864041149603
74-75	20.024857625112944	28.89054400575497	30.400980275902633	20.683618093229455
76-77	20.052756407268472	28.87391101753653	30.21007669090533	20.863255884289668
78-79	20.12546025399051	28.848236546448092	30.50286586918409	20.523437330377302
80-81	19.87226505949096	28.88948187106712	30.511622998969827	20.726630070472094
82-83	19.79859451460012	28.94998690836256	30.68792170616262	20.5634968708747
84-85	19.676641638896836	28.755714147765264	30.500514402354955	21.067129810982944
86-87	19.879670955055236	28.905617114138977	30.30972673697805	20.904985193827738
88-89	19.790963657584086	28.703184244924024	30.34304995952945	21.162802137962437
90-91	19.504812975013586	28.62398821546496	30.428745665786906	21.442453143734554
92-93	19.648554720917556	28.922047675257467	30.261595737872703	21.167801865952274
94-95	19.46213102129758	28.59916693591292	30.46557484276424	21.473127200025267
96-97	19.40190809293648	28.896383107747614	30.25877197111199	21.44293682820392
98-99	19.35409791685851	28.829077573279587	30.23092647607763	21.58589803378427
100	19.447644688128577	28.82729069292884	30.118697831135645	21.60636678780694
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3175.0
1	2133.5
2	1704.0
3	2225.5
4	1395.0
5	978.0
6	2516.5
7	6615.5
8	11021.0
9	12536.5
10	9331.5
11	5264.0
12	4616.5
13	5217.5
14	5986.0
15	6643.0
16	7425.5
17	8615.0
18	10060.0
19	11815.0
20	14458.0
21	18550.5
22	24818.5
23	34080.5
24	45031.0
25	58400.5
26	77368.0
27	100101.0
28	126835.5
29	151921.5
30	170717.5
31	189412.0
32	208556.5
33	224692.0
34	239354.5
35	251495.0
36	256779.5
37	259872.0
38	262141.5
39	259187.0
40	255667.5
41	247969.5
42	234917.0
43	226400.0
44	216660.5
45	206646.0
46	199286.5
47	192056.5
48	185820.5
49	181851.5
50	177841.5
51	163973.0
52	145864.0
53	129711.5
54	118275.5
55	112618.0
56	98483.5
57	82218.5
58	71765.5
59	62862.0
60	50225.5
61	35518.5
62	26289.5
63	19613.5
64	14543.0
65	11007.5
66	8199.5
67	5703.0
68	4019.5
69	2790.5
70	1829.5
71	1193.0
72	831.0
73	601.5
74	501.0
75	378.5
76	231.5
77	157.0
78	117.5
79	71.0
80	45.0
81	31.5
82	17.0
83	15.0
84	10.5
85	4.0
86	1.5
87	1.5
88	0.5
89	0.5
90	0.5
91	0.0
92	1.0
93	1.0
94	0.0
95	0.0
96	0.5
97	0.5
98	0.5
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13162151973130928
2	0.0
3	0.0
4	0.0
5	0.015198193707583412
6	1.9737913905952485E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	2.125621497564114E-4
82-83	6.604609653145639E-4
84-85	3.7957526742216313E-4
86-87	5.389968797394717E-4
88-89	5.76954406481688E-4
90-91	7.591505348443263E-4
92-93	3.112517192861738E-4
94-95	3.188432246346171E-4
96-97	7.136015027536667E-4
98-99	0.0011918663397055924
100	7.591505348443263E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6586309.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.566005063744164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.2661064780363	32.66846067830665
2	17.52495874592629	17.022344703931278
3	6.835003512147584	9.958464455450063
4	3.225102522497061	6.265213817547453
5	1.6788023344576797	4.076636133814859
6	0.9997986726999022	2.913373644064089
7	0.601643039907059	2.045357924588486
8	0.3927547262472307	1.5259622418985965
9	0.26686733011719044	1.1664612095237419
>10	1.0730938598056148	8.762318447205066
>50	0.06959018050288258	2.3427647337847843
>100	0.05693211258980385	5.713384789236735
>500	0.006595731808189976	2.19331185192521
>1k	0.0026257190184754316	2.1051691239539037
>5k	3.1258559686980475E-5	0.1317258292451122
>10k+	9.377567906094142E-5	1.109050415523991
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28961	0.4397151727925307	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27948	0.4243347829565846	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16146	0.24514489071192988	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8677	0.1317429838168844	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.0366021393773052E-5	0.0
5	0.0	0.0	0.0	3.0366021393773052E-5	0.0
6	0.0	0.0	0.0	3.0366021393773052E-5	0.0
7	0.0	0.0	0.0	3.0366021393773052E-5	0.0
8	0.0	0.0	0.0	3.0366021393773052E-5	0.0
9	0.0	0.0	0.0	3.0366021393773052E-5	0.0
10-11	0.0	0.0	0.0	4.554903209065958E-5	0.0
12-13	0.0	0.0	0.0	6.0732042787546103E-5	0.0
14-15	0.0	0.0	0.0	6.832354813598936E-5	0.0
16-17	0.0	0.0	0.0	8.35065588328759E-5	0.0
18-19	0.0	0.0	0.0	1.2146408557509221E-4	0.0
20-21	0.0	0.0	0.0	1.7460462301419505E-4	0.0
22-23	0.0	0.0	0.0	3.4161774067994685E-4	0.0
24-25	0.0	0.0	0.0	8.198825776318724E-4	0.0
26-27	0.0	0.0	0.0	0.0015790331124761988	0.0
28-29	0.0	0.0	0.0	0.003780569663524745	0.0
30-31	0.0	0.0	0.0	0.011136738346166268	0.0
32-33	0.0	0.0	0.0	0.02734460226509263	0.0
34-35	0.0	0.0	0.0	0.05575960678431577	0.0
36-37	0.0	7.591505348443263E-6	0.0	0.09958536716087873	0.0
38-39	0.0	1.5183010696886526E-5	0.0	0.15548162104146646	0.0
40-41	0.0	1.5183010696886526E-5	0.0	0.21294931652918198	0.0
42-43	0.0	1.5183010696886526E-5	0.0	0.2718669895384501	0.0
44-45	0.0	1.5183010696886526E-5	0.0	0.3491864715123448	0.0
46-47	0.0	1.5183010696886526E-5	0.0	0.4455302658894382	0.0
48-49	0.0	1.5183010696886526E-5	0.0	0.5453206036947249	0.0
50-51	0.0	1.5183010696886526E-5	0.0	0.6461661607434452	0.0
52-53	0.0	1.5183010696886526E-5	0.0	0.7459337240326867	0.0
54-55	0.0	1.5183010696886526E-5	0.0	0.8613929288771602	0.0
56-57	0.0	1.5183010696886526E-5	0.0	1.0062692169468515	0.0
58-59	0.0	1.5183010696886526E-5	0.0	1.1668447380771232	0.0
60-61	0.0	1.5183010696886526E-5	0.0	1.334677738320507	0.0
62-63	0.0	1.5183010696886526E-5	0.0	1.505410693606996	0.0
64-65	0.0	1.5183010696886526E-5	0.0	1.6889125608895665	0.0
66-67	0.0	1.5183010696886526E-5	0.0	1.8990985694719151	0.0
68-69	0.0	1.5183010696886526E-5	0.0	2.1399542596619745	0.0
70-71	0.0	1.5183010696886526E-5	0.0	2.4013221973035277	0.0
72-73	0.0	1.5183010696886526E-5	0.0	2.6705245684646743	0.0
74-75	0.0	1.5183010696886526E-5	0.0	2.9449574868108983	0.0
76-77	0.0	1.5183010696886526E-5	0.0	3.239766005512344	0.0
78-79	0.0	1.5183010696886526E-5	0.0	3.576487225242545	0.0
80-81	0.0	1.5183010696886526E-5	0.0	3.9444626724922864	0.0
82-83	0.0	1.5183010696886526E-5	0.0	4.321563109170857	0.0
84-85	0.0	1.5183010696886526E-5	0.0	4.703074210456874	0.0
86-87	0.0	1.5183010696886526E-5	0.0	5.107853275635868	0.0
88	0.0	1.5183010696886526E-5	0.0	5.434591665832866	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15255	0.0	63.715473	1
GTATCAA	29265	0.0	41.576614	1
TGGTATC	5005	0.0	39.82003	2
GTGGTAT	5560	0.0	37.503	1
ATCAACG	36555	0.0	32.853676	3
TCAACGC	36995	0.0	32.57728	4
TATCAAC	37830	0.0	31.90792	2
CAACGCA	39245	0.0	30.709555	5
AACGCAG	41220	0.0	29.30301	6
CGCAGAG	43855	0.0	27.188702	8
ACGCAGA	44935	0.0	26.671198	7
GCAGAGT	47090	0.0	25.101309	9
GAGTACT	36070	0.0	22.632929	12-13
GTACATG	22135	0.0	22.178995	1
CAGAGTA	47650	0.0	22.158003	10-11
TACATGG	21680	0.0	21.61602	2
AGAGTAC	46400	0.0	20.946894	10-11
GTACTTT	39540	0.0	20.557533	14-15
ACATGGG	21985	0.0	20.396788	3
AGTACTT	37355	0.0	20.030022	12-13
>>END_MODULE