FastQCFastQC Report
Wed 25 May 2016
SRR2049452_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049452_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5939009
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62300.040.4577451
GTATCAA101150.033.6122321
TCAACGC104300.031.2271544
ATCAACG104500.031.0784873
CAACGCA113650.028.5770685
AACGCAG122550.026.6167626
TATCAAC124150.026.5380732
GTACATG180850.023.7918131
ACGCAGA140250.023.056767
TACATGG182550.022.7855512
CGCAGAG141600.022.7012988
ACATGGG185150.021.6024953
TAGACCG2702.6002454E-819.1486435
CATGGGG128250.018.8726674
GCAGAGT165350.018.7875339
GTCGTAC4550.018.6031321
AGAGTAC145400.017.30882310-11
CAGAGTA157250.016.67692610-11
GAGTACA131200.016.4155941
GTGGTAT39750.016.0889131
Produced by FastQC (version 0.11.5)