##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049452_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5939009
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.90360967629448	31.0	31.0	34.0	28.0	34.0
2	30.964136946079726	31.0	31.0	34.0	28.0	34.0
3	31.158991171759464	31.0	31.0	34.0	28.0	34.0
4	34.75312396394752	37.0	35.0	37.0	32.0	37.0
5	34.79752952049744	37.0	35.0	37.0	32.0	37.0
6	34.840892478863054	37.0	35.0	37.0	32.0	37.0
7	34.67526585664376	37.0	35.0	37.0	32.0	37.0
8	34.6887179325709	37.0	35.0	37.0	32.0	37.0
9	36.24502976843443	39.0	37.0	39.0	32.0	39.0
10-11	36.22067747329563	39.0	37.0	39.0	32.0	39.0
12-13	36.16200985720008	39.0	37.0	39.0	32.0	39.0
14-15	37.39074902900467	40.0	38.0	41.0	32.0	41.0
16-17	37.36713372550875	40.0	38.0	41.0	32.0	41.0
18-19	37.36077273161229	40.0	38.0	41.0	32.0	41.0
20-21	37.422691058390384	40.0	38.0	41.0	32.0	41.0
22-23	37.369587249320546	40.0	38.0	41.0	32.0	41.0
24-25	37.366964505357714	40.0	38.0	41.0	32.0	41.0
26-27	37.37313152749895	40.0	38.0	41.0	32.0	41.0
28-29	37.35548220250213	40.0	38.0	41.0	32.0	41.0
30-31	37.30564131153868	40.0	38.0	41.0	32.0	41.0
32-33	37.280110957905606	40.0	38.0	41.0	31.0	41.0
34-35	37.221377505910496	40.0	37.5	41.0	31.0	41.0
36-37	37.112835239010415	40.0	37.0	41.0	31.0	41.0
38-39	37.00949325047327	40.0	37.0	41.0	30.5	41.0
40-41	36.89796605797365	40.0	37.0	41.0	30.0	41.0
42-43	36.798628609587894	40.0	37.0	41.0	30.0	41.0
44-45	36.63191704205197	40.0	37.0	41.0	30.0	41.0
46-47	36.517056465144265	40.0	36.0	41.0	30.0	41.0
48-49	36.42423693919305	40.0	36.0	41.0	30.0	41.0
50-51	36.310808251006186	40.0	36.0	41.0	30.0	41.0
52-53	36.208911200505	39.5	36.0	41.0	29.5	41.0
54-55	36.07097783485426	39.0	35.0	41.0	29.0	41.0
56-57	35.91578384541933	39.0	35.0	41.0	29.0	41.0
58-59	35.74333192625234	39.0	35.0	41.0	28.5	41.0
60-61	35.56023681728719	39.0	35.0	40.5	28.0	41.0
62-63	35.317593473928056	38.0	35.0	40.0	28.0	41.0
64-65	35.08784512702371	38.0	34.0	40.0	28.0	41.0
66-67	34.87215198697291	37.5	34.0	40.0	28.0	41.0
68-69	34.5943846018755	37.0	34.0	40.0	28.0	41.0
70-71	34.289948878676555	36.5	34.0	39.5	27.0	41.0
72-73	33.96185289498635	36.0	34.0	39.0	26.0	41.0
74-75	33.66156491764872	36.0	34.0	39.0	26.0	40.5
76-77	33.33443197341509	35.0	33.5	38.5	26.0	40.0
78-79	32.98808454407124	35.0	33.0	37.0	26.0	39.0
80-81	32.654960364599546	35.0	33.0	37.0	26.0	39.0
82-83	32.35445172755253	35.0	33.0	36.5	25.5	39.0
84-85	32.06663106588995	35.0	33.0	36.0	25.0	37.0
86-87	31.796610764523173	35.0	33.0	36.0	25.0	37.0
88-89	31.568333959419828	35.0	33.0	35.0	24.5	37.0
90-91	31.365944385671078	35.0	32.0	35.0	24.0	36.0
92-93	31.164387274038482	35.0	32.0	35.0	23.5	36.0
94-95	30.965176008320583	35.0	32.0	35.0	22.0	36.0
96-97	30.782615921275756	35.0	32.0	35.0	20.0	36.0
98-99	30.613463205730113	35.0	32.0	35.0	18.5	35.0
100	30.483418361548196	35.0	32.0	35.0	17.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.11387417563570068
1101	2	0.11210731924052197
1101	3	0.17649186286672247
1101	4	0.15717564876287327
1101	5	0.2710097107309579
1101	6	0.1892598321570489
1101	7	0.2132773518613149
1101	8	0.20901487956592035
1101	9	0.2993107839777025
1101	10-11	0.36475256073889994
1101	12-13	0.3280554995646767
1101	14-15	0.4477400603148993
1101	16-17	0.34778774669689483
1101	18-19	0.34616933976968056
1101	20-21	0.32903785096775806
1101	22-23	0.31597909873453744
1101	24-25	0.32887784100897477
1101	26-27	0.30471137010573557
1101	28-29	0.3374545966565279
1101	30-31	0.27356327225328414
1101	32-33	0.2875604204566926
1101	34-35	0.26099376514037687
1101	36-37	0.2867251379431721
1101	38-39	0.2840238843354257
1101	40-41	0.36375873466182895
1101	42-43	0.233844952546292
1101	44-45	0.24403077292527797
1101	46-47	0.14566419113827322
1101	48-49	0.15972853742612614
1101	50-51	0.1713007123515382
1101	52-53	0.1887623843491184
1101	54-55	0.19345966530742942
1101	56-57	0.19480374201020823
1101	58-59	0.2086473666452875
1101	60-61	0.19415592956249839
1101	62-63	0.1765842466299148
1101	64-65	0.19716992733685856
1101	66-67	0.21449365065942771
1101	68-69	0.12641043182316025
1101	70-71	0.14958281422235586
1101	72-73	0.14944669569744207
1101	74-75	0.10737605679973683
1101	76-77	0.09945850236981357
1101	78-79	0.05095997638448324
1101	80-81	0.15816580252808166
1101	82-83	0.1162381947591129
1101	84-85	0.10789865448413138
1101	86-87	0.09211837947649926
1101	88-89	0.08046063384690072
1101	90-91	0.11739847163599038
1101	92-93	0.13861343193044107
1101	94-95	0.0680831881019408
1101	96-97	0.06960270989534934
1101	98-99	0.09102275213407296
1101	100	0.09670973920455594
1102	1	0.03643225515440207
1102	2	0.08810877961868968
1102	3	0.16158526384027638
1102	4	0.1338147084800525
1102	5	0.1574351075339564
1102	6	0.0800366389815963
1102	7	0.0752173603146602
1102	8	0.167078305319869
1102	9	0.17786231408449993
1102	10-11	0.28790484296622054
1102	12-13	0.2570510096130576
1102	14-15	0.32734501054661536
1102	16-17	0.23011421660005738
1102	18-19	0.2339887701919494
1102	20-21	0.24994970076946998
1102	22-23	0.24230863875853714
1102	24-25	0.21688789037765588
1102	26-27	0.19405278385104197
1102	28-29	0.19120277181387024
1102	30-31	0.14666759766873128
1102	32-33	0.18185815782820924
1102	34-35	0.19114481285642881
1102	36-37	0.19673860202952653
1102	38-39	0.20258964187244288
1102	40-41	0.15515305754530573
1102	42-43	0.14553297771972495
1102	44-45	0.046475058204414665
1102	46-47	0.06991075590889295
1102	48-49	-0.025379561977040055
1102	50-51	0.02504117286349583
1102	52-53	0.021998304533255464
1102	54-55	0.009063028422382047
1102	56-57	-0.015345116202674092
1102	58-59	-0.015144806912786635
1102	60-61	-0.029108714864591434
1102	62-63	-0.09673303100026232
1102	64-65	-0.05274055617455531
1102	66-67	-0.02293650379757395
1102	68-69	-0.06952478033294796
1102	70-71	-0.08947455636103996
1102	72-73	-0.08626910634369267
1102	74-75	-0.06394820450266536
1102	76-77	-0.0960178564004579
1102	78-79	-0.092449316663334
1102	80-81	-0.09198837136948868
1102	82-83	-0.11598294993297031
1102	84-85	-0.13595420127037272
1102	86-87	-0.16849443834939137
1102	88-89	-0.14932252189277762
1102	90-91	-0.12753338438335504
1102	92-93	-0.13749124788265377
1102	94-95	-0.11849520930047319
1102	96-97	-0.08213806413084157
1102	98-99	-0.1042683851413031
1102	100	-0.16415515570909278
1103	1	0.23748322271523747
1103	2	0.22865848188293825
1103	3	0.2692376297627632
1103	4	0.27021137735376044
1103	5	0.26436675794782616
1103	6	0.2684622821829592
1103	7	0.3024186656807615
1103	8	0.32391878152564857
1103	9	0.3613196057712571
1103	10-11	0.3674233767221011
1103	12-13	0.38060840008080277
1103	14-15	0.408545633445371
1103	16-17	0.41545761445173923
1103	18-19	0.3900560869703611
1103	20-21	0.44571344814726643
1103	22-23	0.42787413312416334
1103	24-25	0.37646956575461843
1103	26-27	0.36107005873860487
1103	28-29	0.3736879760933789
1103	30-31	0.3445950182850268
1103	32-33	0.3781518084177904
1103	34-35	0.3419766569753122
1103	36-37	0.3050874167648203
1103	38-39	0.2769467083556876
1103	40-41	0.36442124792476704
1103	42-43	0.26196544130896626
1103	44-45	0.22393344055325315
1103	46-47	0.16863522766632144
1103	48-49	0.13039952752367867
1103	50-51	0.12129290581627572
1103	52-53	0.18440766601381142
1103	54-55	0.13830013814641973
1103	56-57	0.06625258590729999
1103	58-59	0.10922380366084639
1103	60-61	0.07327175157023191
1103	62-63	0.08667382560016534
1103	64-65	0.13328763926180898
1103	66-67	0.13039341719906616
1103	68-69	0.12975099539779222
1103	70-71	0.07164923271476198
1103	72-73	0.1038707941039334
1103	74-75	0.1350931806596165
1103	76-77	0.05648761298065352
1103	78-79	0.10724534080662806
1103	80-81	0.11420051994785752
1103	82-83	0.052796526334951466
1103	84-85	0.006697061133493776
1103	86-87	0.10515439417076422
1103	88-89	0.08030247998213369
1103	90-91	0.0224032168650119
1103	92-93	0.0055397374201930916
1103	94-95	0.018448317330754804
1103	96-97	0.0297723569418622
1103	98-99	0.06950362898373186
1103	100	0.07860683353856501
1104	1	-0.024287907770165873
1104	2	-0.053375572098005364
1104	3	0.007032017628855414
1104	4	-0.0034352677238231877
1104	5	0.019707755458242104
1104	6	0.016120383155907803
1104	7	-1.100550553019275E-4
1104	8	0.012558553374574899
1104	9	0.04087582610748086
1104	10-11	0.10608334655498197
1104	12-13	0.11425491144798627
1104	14-15	0.10817580205932842
1104	16-17	0.07754173011945653
1104	18-19	0.05022611259646936
1104	20-21	0.03955495941416842
1104	22-23	0.030286782950518898
1104	24-25	0.08613640958756719
1104	26-27	0.07549023646028985
1104	28-29	0.07351505897993604
1104	30-31	0.07926765409236936
1104	32-33	0.008424028642927794
1104	34-35	0.05115246223332548
1104	36-37	0.0609559917874094
1104	38-39	0.04934364978178962
1104	40-41	0.05271687621191745
1104	42-43	0.035016149434085264
1104	44-45	0.03297294477567192
1104	46-47	0.026059124594119965
1104	48-49	-0.019097689242990157
1104	50-51	-0.034718649333342455
1104	52-53	-0.020144729433653197
1104	54-55	-0.09122093821198973
1104	56-57	-0.06650203854391634
1104	58-59	-0.03680178979474391
1104	60-61	-0.052846035203856445
1104	62-63	-0.04152103647498251
1104	64-65	-0.004158259454627
1104	66-67	0.02708529984659691
1104	68-69	-0.024593583237042083
1104	70-71	-0.016273478561799948
1104	72-73	-0.04557817763740246
1104	74-75	-0.06022708402525723
1104	76-77	-0.08428597109053015
1104	78-79	-0.09593285246763372
1104	80-81	-0.09229894315347309
1104	82-83	-0.06524505605558772
1104	84-85	-0.10436381001030526
1104	86-87	-0.13430066396497153
1104	88-89	-0.1408933628073541
1104	90-91	-0.11484426333684894
1104	92-93	-0.14530378875953076
1104	94-95	-0.19749279686769583
1104	96-97	-0.18676289426139903
1104	98-99	-0.175381376218116
1104	100	-0.21094465036399157
1105	1	0.09350449308486475
1105	2	0.09544433333784852
1105	3	0.12216741132844433
1105	4	0.06147690033417064
1105	5	0.09536867222013257
1105	6	0.1534256427672389
1105	7	0.15038703751291393
1105	8	0.14301989655878344
1105	9	0.24223998057846075
1105	10-11	0.19809593251948598
1105	12-13	0.22141135805825485
1105	14-15	0.24417540769536572
1105	16-17	0.20174785836904618
1105	18-19	0.16774765136977265
1105	20-21	0.1555711122473511
1105	22-23	0.16738526211807425
1105	24-25	0.13156700160516266
1105	26-27	0.16771026699191793
1105	28-29	0.2263139278951769
1105	30-31	0.21680240591297917
1105	32-33	0.20681658824599225
1105	34-35	0.19047213953653852
1105	36-37	0.16957004504849493
1105	38-39	0.17216239929216215
1105	40-41	0.19530633976715706
1105	42-43	0.14609024088953504
1105	44-45	0.0928233150282054
1105	46-47	0.06728929305639753
1105	48-49	0.11931709493471487
1105	50-51	0.11890768594930279
1105	52-53	0.13413538516446266
1105	54-55	0.04322077814738634
1105	56-57	0.10625264687137559
1105	58-59	0.0855900850344895
1105	60-61	0.12554746469762534
1105	62-63	0.1204118842598163
1105	64-65	0.08267334988967434
1105	66-67	0.06826651740573908
1105	68-69	0.048758301943500726
1105	70-71	0.05917948614247592
1105	72-73	0.07549094517293753
1105	74-75	0.05466737700055546
1105	76-77	0.024245994018379236
1105	78-79	0.0783334278145773
1105	80-81	0.09198650247554951
1105	82-83	0.0679401577217078
1105	84-85	0.006938783734710796
1105	86-87	0.02631426639004175
1105	88-89	-0.0028527620148075528
1105	90-91	0.054920012470720536
1105	92-93	-0.01479871994457227
1105	94-95	0.018588763344006054
1105	96-97	-0.07758699891160248
1105	98-99	-0.06814268482280283
1105	100	-0.11254392454984696
1106	1	0.005022788566680703
1106	2	-0.052744671431746326
1106	3	0.010216077729484851
1106	4	0.06365432363504908
1106	5	0.07767929231393822
1106	6	0.06608056136725793
1106	7	0.09172213934736106
1106	8	0.05059823836181465
1106	9	0.1293125054341715
1106	10-11	0.11739976869896651
1106	12-13	0.10534407987233152
1106	14-15	0.1750376306243666
1106	16-17	0.1943855113653541
1106	18-19	0.15469694618287377
1106	20-21	0.08767073256716884
1106	22-23	0.1027483232793216
1106	24-25	0.09171616500394464
1106	26-27	0.07771774933603126
1106	28-29	0.04266628659566152
1106	30-31	0.05929833416635688
1106	32-33	0.04651675857198967
1106	34-35	0.06597047705229642
1106	36-37	0.10175704190527313
1106	38-39	0.06410543794520862
1106	40-41	0.16548465489122322
1106	42-43	0.04591915763018051
1106	44-45	-0.09077794389293814
1106	46-47	-0.06962202208863744
1106	48-49	-0.08006480344288036
1106	50-51	-0.0804092625358237
1106	52-53	-0.09450185250204868
1106	54-55	-0.10998618832564944
1106	56-57	-0.13025357608557897
1106	58-59	-0.10664302915848367
1106	60-61	-0.09070225569745105
1106	62-63	-0.045007992038314626
1106	64-65	-0.060276484910417594
1106	66-67	-0.02161117291783654
1106	68-69	-0.04776415557402913
1106	70-71	-0.038095501799567444
1106	72-73	-0.024416697827504663
1106	74-75	-0.0010007972026286893
1106	76-77	-0.04888336170198926
1106	78-79	-0.035704675928172946
1106	80-81	0.007404995311837581
1106	82-83	-0.0015413732084255116
1106	84-85	-0.03522869611625623
1106	86-87	-0.03074399570228792
1106	88-89	-0.005554371723622609
1106	90-91	-0.06286836505184112
1106	92-93	-0.21065715946952324
1106	94-95	-0.17409543222099444
1106	96-97	-0.0948582820884738
1106	98-99	-0.09587774518251635
1106	100	-0.06998863850236603
1107	1	0.07068579910038864
1107	2	0.06909139467298431
1107	3	0.11839562637899093
1107	4	0.09480996318651336
1107	5	0.08629321391666167
1107	6	0.17527029123807125
1107	7	0.12258888511059496
1107	8	0.10416675141066634
1107	9	0.13618993908377774
1107	10-11	0.14958770279727673
1107	12-13	0.17232561519816159
1107	14-15	0.19521834858106502
1107	16-17	0.1420075191530401
1107	18-19	0.19511383114167558
1107	20-21	0.20669174155228376
1107	22-23	0.16871073971746853
1107	24-25	0.158501462322576
1107	26-27	0.2655248144062199
1107	28-29	0.1879140074552197
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2216	20-21	0.1063075168891956
2216	22-23	0.08286526552820561
2216	24-25	0.15367151823073044
2216	26-27	0.07667582387971805
2216	28-29	0.15059320516625263
2216	30-31	0.084562798015547
2216	32-33	0.06807113736948622
2216	34-35	0.09856746757791512
2216	36-37	0.09755999141713545
2216	38-39	0.17916220297494334
2216	40-41	0.18447776380217817
2216	42-43	0.2751795636577157
2216	44-45	0.31014553258365396
2216	46-47	0.4732991417669936
2216	48-49	0.32218747160565897
2216	50-51	0.33811585884610906
2216	52-53	0.27024469083505664
2216	54-55	0.2623065460879275
2216	56-57	0.3565849000689667
2216	58-59	0.3814334199868554
2216	60-61	0.3843098019831146
2216	62-63	0.32375592509379914
2216	64-65	0.3654163237278638
2216	66-67	0.3597843818811626
2216	68-69	0.4372783204333004
2216	70-71	0.464672320564155
2216	72-73	0.4643660553569475
2216	74-75	0.4127063127266126
2216	76-77	0.43804201026500067
2216	78-79	0.4407552235617658
2216	80-81	0.46543686746132096
2216	82-83	0.4259190837335751
2216	84-85	0.48918797285075755
2216	86-87	0.4100022807939361
2216	88-89	0.46424159851902047
2216	90-91	0.40383713923141684
2216	92-93	0.409199827620899
2216	94-95	0.5361113847967083
2216	96-97	0.5087365936464892
2216	98-99	0.45310810611657004
2216	100	0.39815286077532264
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	96406.0
3	18411.0
4	11255.0
5	9122.0
6	7739.0
7	7279.0
8	7694.0
9	8764.0
10	9885.0
11	11187.0
12	12392.0
13	13643.0
14	15114.0
15	16209.0
16	16267.0
17	17172.0
18	16389.0
19	15466.0
20	15524.0
21	17081.0
22	19517.0
23	23822.0
24	29118.0
25	35511.0
26	43659.0
27	50691.0
28	60781.0
29	76499.0
30	93879.0
31	110094.0
32	142273.0
33	190025.0
34	267516.0
35	397128.0
36	669131.0
37	1198166.0
38	1613311.0
39	573797.0
40	1092.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.584963589380216	20.820201553823097	14.753567971124268	22.84126688567242
2	14.973370185823512	20.669912393662194	40.889895691825345	23.46682172868895
3	17.961105288941738	25.877028934338668	30.744012619671402	25.417853157048185
4	12.462573917565285	17.535546779487714	39.2235784487735	30.7783008541735
5	12.923613293201946	38.27226229411026	35.55778619966611	13.246338213021685
6	29.950692281579034	38.428565270338716	18.248183417572825	13.372559030509423
7	26.380323360981787	33.087359065956825	22.9295486405595	17.60276893250188
8	24.26821129992993	36.905179879690095	20.903420511957822	17.923188308422148
9	24.499737224487745	17.703778157856938	22.08159630103684	35.71488831661848
10-11	23.25199207715912	27.655796953794994	29.387049166138173	19.705161802907714
12-13	23.7113047027891	25.263772918095633	29.33256059076916	21.692361788346116
14-15	21.27858529395792	27.491053051897563	26.43460381890813	24.795757835236387
16-17	20.838418583527083	30.795132877745434	27.077861646224203	21.28858689250328
18-19	20.856380791880326	29.790766669672447	28.929809011828873	20.423043526618358
20-21	21.839295797156385	29.008625211740284	28.502039897350905	20.650039093752426
22-23	21.597537876236384	28.847034700910427	28.531169252821687	21.024258170031498
24-25	21.40278410692652	28.89903629658745	28.50667398209606	21.191505614389975
26-27	21.197153464992258	28.98644642649968	28.502134042486194	21.31426606602187
28-29	21.1785783238255	28.962618316756195	28.66393262976522	21.19487072965308
30-31	21.466972470112175	29.175004272996937	28.366098589616325	20.99192466727457
32-33	21.137970218683495	29.04452142799166	28.61394756863602	21.203560784688822
34-35	21.360773974813167	28.827828795278705	28.608717682184313	21.20267954772381
36-37	21.551889120177595	29.010389973638812	28.28303877491584	21.154682131267748
38-39	21.34866097956674	29.119931545300677	28.386882895360287	21.144524579772302
40-41	21.340550673586055	28.85250992246759	28.56337791462224	21.24356148932412
42-43	21.306080564418043	28.890801617963362	28.462475101358457	21.34064271626014
44-45	21.361600350025622	29.02494522008046	28.363359067110306	21.250095362783604
46-47	21.437520445638075	28.97814542853426	28.30855999134389	21.275774134483775
48-49	21.38192534321585	28.877445319800664	28.32828143085237	21.41234790613111
50-51	21.354052869151875	28.92938371917874	28.269346556304598	21.447216855364783
52-53	21.344474079981598	28.932340996567955	28.255825055768003	21.467359867682447
54-55	21.47092133838489	29.10521773959219	28.163611996666088	21.26024892535683
56-57	21.32449907311	28.97715383511	28.211239135935585	21.48710795584442
58-59	21.36034941103651	28.69216024225329	28.387979743077345	21.559510603632855
60-61	21.505906406791052	28.99875493295093	28.12171852256983	21.37362013768818
62-63	21.37238890560747	28.990610071687055	28.22786679602187	21.409134226683605
64-65	21.446469403665404	28.840909906203205	28.256442013917017	21.45617867621437
66-67	21.30111919179604	28.87795553712904	28.232889093062447	21.588036178012473
68-69	21.455604509561525	28.99644119364248	28.171952672068617	21.37600162472738
70-71	21.51256326304382	28.77107361135824	28.20062518425976	21.515737941338177
72-73	21.39798218021406	28.89409949049276	28.314761092063957	21.393157237229225
74-75	21.421779669066407	28.972174861414906	28.18327217782544	21.422773291693247
76-77	21.49095605712212	28.921209967828602	28.03455583518074	21.55327813986854
78-79	21.484526391895574	28.888020152952347	28.26311124463038	21.3643422105217
80-81	21.387940717330505	28.866943571963816	28.23170956082804	21.513406149877635
82-83	21.367749062453164	28.898839243765543	28.348587238585672	21.384824455195616
84-85	21.391523159804876	28.811915750930556	28.22327997208672	21.57328111717785
86-87	21.521515444438084	28.98071929215939	28.09586801076446	21.401897252638065
88-89	21.349832208760848	28.93946085355241	28.137363578839146	21.573343358847595
90-91	21.308728237001226	28.819548322022705	28.22512041425721	21.646603026718857
92-93	21.407026502514277	28.967342823524955	28.147198302626673	21.47843237133409
94-95	21.330870952963263	28.8307911723108	28.260075328092142	21.57826254663379
96-97	21.35599853559945	28.90920514507097	28.178629923280635	21.556166396048948
98-99	21.29606132489704	28.928019999767617	28.139306612529257	21.636612062806087
100	21.398961374864868	28.858303781013095	28.119226352250188	21.62350849187185
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4036.0
1	3051.0
2	2348.5
3	2622.0
4	2357.0
5	1953.0
6	1727.0
7	1636.5
8	1672.5
9	1745.5
10	1560.0
11	1311.0
12	1283.0
13	1351.0
14	1427.5
15	1590.0
16	1890.0
17	2373.5
18	3143.5
19	4225.0
20	6213.0
21	9024.0
22	12584.5
23	18342.0
24	26544.0
25	35228.0
26	46763.5
27	62432.0
28	80827.5
29	100179.5
30	117965.0
31	135162.0
32	150643.0
33	164265.5
34	177249.0
35	188907.0
36	197836.0
37	204803.5
38	213459.0
39	220355.5
40	222708.0
41	221865.5
42	216882.0
43	211297.5
44	205790.5
45	199767.0
46	195483.0
47	188090.0
48	179966.0
49	175695.5
50	169632.0
51	161529.0
52	152736.5
53	144104.5
54	137754.5
55	130569.0
56	118891.0
57	105850.5
58	95089.0
59	86297.0
60	75901.5
61	64374.5
62	54347.5
63	45066.5
64	37225.0
65	30418.0
66	24087.0
67	18938.0
68	14858.0
69	11207.0
70	8314.5
71	6316.5
72	4921.0
73	3718.5
74	2749.0
75	1992.5
76	1341.5
77	880.5
78	615.5
79	461.0
80	297.0
81	193.5
82	141.5
83	101.5
84	80.0
85	64.5
86	50.5
87	36.0
88	42.0
89	31.0
90	15.0
91	16.0
92	13.0
93	15.0
94	13.0
95	5.5
96	7.5
97	9.0
98	11.5
99	13.5
100	29.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07768972904402065
2	0.012459991220757537
3	0.016467393802568744
4	0.01072569514543588
5	0.008671480376608287
6	0.0064825629999887184
7	0.018942554220746255
8	0.008620966898686296
9	0.008082156467518403
10-11	0.012375802090887553
12-13	0.014345827729845165
14-15	0.008949304505179232
16-17	0.011357113619460756
18-19	0.007534927123363511
20-21	0.012123234701277604
22-23	0.0121400725272516
24-25	0.01473309772724709
26-27	0.0047145912727190676
28-29	0.011104546229850805
30-31	0.009328155589594156
32-33	0.01102877601296782
34-35	0.007905359294791438
36-37	0.00915135841686719
38-39	0.017974379227241447
40-41	0.012089559049329611
42-43	0.007273940820766562
44-45	0.019498202477888145
46-47	0.01694727184282765
48-49	0.016534745106464733
50-51	0.009353412328555152
52-53	0.00956388515323011
54-55	0.010818303188292862
56-57	0.0192203783493172
58-59	0.001700620423373664
60-61	0.006280509088300759
62-63	0.01411009816620921
64-65	0.023337226799959386
66-67	0.01188750513764165
68-69	0.013167179911665399
70-71	0.023564537450608342
72-73	0.01877417596100629
74-75	0.019211959436330202
76-77	0.008486264290894323
78-79	0.010498384494786924
80-81	0.018833108351915276
82-83	0.010759370797383873
84-85	0.010388938625955945
86-87	0.015979096849322843
88-89	0.013916463167508249
90-91	0.01452262490257213
92-93	0.007097143648039597
94-95	0.014354246642832162
96-97	0.012510504698679526
98-99	0.009092426025958203
100	0.006549914303884704
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5939009.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.45839876722315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.39939521151099	44.83127307293477
2	14.5108564269502	17.25584575775056
3	4.5241259704149925	8.06991858066255
4	1.9264246796090703	4.581685071808648
5	1.0219953280800034	3.038310287760995
6	0.5874886927458878	2.0958682178711783
7	0.4032128624283212	1.678207381363563
8	0.2881223363955621	1.3705034216921077
9	0.20779016396642985	1.11193833861204
>10	1.0498544464864636	10.957015919600192
>50	0.057239727917425424	2.321856571212669
>100	0.022531102629518452	2.2370341892006587
>500	8.214294293016944E-4	0.29595811211049633
>1k	1.4162143582564823E-4	0.15458507741949173
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.6837825973996672E-5	0.0
2	0.0	0.0	0.0	1.6837825973996672E-5	0.0
3	0.0	0.0	0.0	1.6837825973996672E-5	0.0
4	0.0	0.0	0.0	1.6837825973996672E-5	0.0
5	0.0	0.0	0.0	1.6837825973996672E-5	0.0
6	0.0	0.0	0.0	1.6837825973996672E-5	0.0
7	0.0	1.6837825973996672E-5	0.0	1.6837825973996672E-5	0.0
8	0.0	1.6837825973996672E-5	0.0	1.6837825973996672E-5	0.0
9	0.0	1.6837825973996672E-5	0.0	8.418912986998336E-5	0.0
10-11	0.0	1.6837825973996672E-5	0.0	8.418912986998336E-5	0.0
12-13	0.0	1.6837825973996672E-5	0.0	9.26080428569817E-5	0.0
14-15	0.0	3.3675651947993345E-5	0.0	1.0102695584398003E-4	0.0
16-17	0.0	5.051347792199002E-5	0.0	1.0102695584398003E-4	0.0
18-19	0.0	6.735130389598669E-5	0.0	1.0102695584398003E-4	0.0
20-21	0.0	1.178647818179767E-4	0.0	1.4312152077897172E-4	0.0
22-23	0.0	1.3470260779197338E-4	0.0	1.683782597399667E-4	0.0
24-25	0.0	1.5154043376597006E-4	0.0	3.199186935059368E-4	0.0
26-27	0.0	1.683782597399667E-4	0.0	5.388104311678935E-4	0.0
28-29	0.0	1.683782597399667E-4	0.0	0.0011702289051927686	0.0
30-31	0.0	1.683782597399667E-4	0.0	0.0028708493285664327	0.0
32-33	0.0	1.683782597399667E-4	0.0	0.0065835899558326985	0.0
34-35	0.0	1.683782597399667E-4	0.0	0.012872517957120456	0.0
36-37	0.0	2.1047282467495838E-4	0.0	0.021611349637624726	0.0
38-39	0.0	2.525673896099501E-4	0.0	0.03226969347916462	0.0
40-41	0.0	2.6940521558394676E-4	0.0	0.04399723927005331	0.0
42-43	0.0	3.367565194799334E-4	0.0	0.05575846071288998	0.0
44-45	0.0	3.367565194799334E-4	0.0	0.07063467996091605	0.0
46-47	0.0	3.535943454539301E-4	0.0	0.08895423462062442	0.0
48-49	0.0	3.704321714279268E-4	0.0	0.10803991036214963	0.0
50-51	0.0	4.0410782337592014E-4	0.0	0.12581223567770314	0.0
52-53	0.0	4.209456493499168E-4	0.0	0.14336566925559469	0.0
54-55	0.0	4.209456493499168E-4	0.0	0.16451397867893447	0.0
56-57	0.0	4.209456493499168E-4	0.0	0.1900990552464224	0.0
58-59	0.0	4.3778347532391344E-4	0.0	0.2201293178710455	0.0
60-61	0.0	4.462023883109118E-4	0.0	0.25033637766839556	0.0
62-63	0.0	4.6304021428490846E-4	0.0	0.28106541007093944	0.0
64-65	0.0	4.714591272719068E-4	0.0	0.3147158052799718	0.0
66-67	0.0	4.967158662329018E-4	0.0	0.35387216958250106	0.0
68-69	0.0	5.051347792199002E-4	0.0	0.3986102731954102	0.0
70-71	0.0	5.051347792199002E-4	0.0	0.4472126578693516	0.0
72-73	0.0	5.051347792199002E-4	0.0	0.4987195675238074	0.0
74-75	0.0	5.051347792199002E-4	0.0	0.5525500971626748	0.0
76-77	0.0	5.219726051938968E-4	0.0	0.612442244152181	0.0
78-79	0.0	5.219726051938968E-4	0.0	0.682916964766344	0.0
80-81	0.0	5.303915181808952E-4	0.0	0.7606487883752997	0.0
82-83	0.0	5.388104311678935E-4	0.0	0.8428426358673644	0.0
84-85	0.0	5.388104311678935E-4	0.0	0.9289260211594224	0.0
86-87	0.0	5.388104311678935E-4	0.0	1.0233946437865307	0.0
88	0.0	5.388104311678935E-4	0.0	1.1013116834812002	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6230	0.0	40.457745	1
GTATCAA	10115	0.0	33.612232	1
TCAACGC	10430	0.0	31.227154	4
ATCAACG	10450	0.0	31.078487	3
CAACGCA	11365	0.0	28.577068	5
AACGCAG	12255	0.0	26.616762	6
TATCAAC	12415	0.0	26.538073	2
GTACATG	18085	0.0	23.791813	1
ACGCAGA	14025	0.0	23.05676	7
TACATGG	18255	0.0	22.785551	2
CGCAGAG	14160	0.0	22.701298	8
ACATGGG	18515	0.0	21.602495	3
TAGACCG	270	2.6002454E-8	19.148643	5
CATGGGG	12825	0.0	18.872667	4
GCAGAGT	16535	0.0	18.787533	9
GTCGTAC	455	0.0	18.603132	1
AGAGTAC	14540	0.0	17.308823	10-11
CAGAGTA	15725	0.0	16.676926	10-11
GAGTACA	13120	0.0	16.415594	1
GTGGTAT	3975	0.0	16.088913	1
>>END_MODULE