FastQCFastQC Report
Wed 25 May 2016
SRR2049452_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049452_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5939009
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210260.35403212892925406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191490.32242752957606224No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122420.20612866557366727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107150.057.7506941
GTATCAA198200.041.0739141
ATCAACG242850.032.9235653
TCAACGC244150.032.825274
CAACGCA254400.031.5396675
TATCAAC256600.031.342532
AACGCAG264550.030.3968146
GTGGTAT44600.026.799241
ACGCAGA300650.026.6375477
CGCAGAG303350.026.3229898
TGGTATC47200.023.9006612
GTACATG172300.023.5421241
GCAGAGT334900.023.52049
TACATGG173150.022.206062
ACATGGG171750.021.8670773
CAGAGTA321850.021.36370810-11
GAGTACT244600.021.34732412-13
AGAGTAC311900.020.88496210-11
AGTACTT257250.019.8591212-13
CATGGGG118800.019.4274
Produced by FastQC (version 0.11.5)