FastQCFastQC Report
Wed 25 May 2016
SRR2049451_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049451_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5929648
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75150.049.3727951
GTATCAA127000.036.1768341
ATCAACG141450.031.7647483
TCAACGC141300.031.6313614
TATCAAC156700.029.0033362
CAACGCA155550.028.8244845
AACGCAG173950.025.939146
GTACATG225700.024.8361551
TACATGG226200.024.24762
CGCAGAG184450.023.5937618
ACGCAGA194450.022.7939667
GTGGTAT41200.022.5999621
ACATGGG237550.022.4163743
GCAGAGT205000.020.4038439
TGGTATC43100.020.0646252
GTCGTAC2902.7685019E-919.4591081
CATGGGG142300.019.3870934
CATGGGT73300.019.0428524
CAGAGTA199250.018.42196310-11
AGAGTAC182050.017.18068910-11
Produced by FastQC (version 0.11.5)