##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049451_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5929648
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.496405520192766	31.0	31.0	34.0	27.0	34.0
2	30.740979059802537	31.0	31.0	34.0	27.0	34.0
3	30.805386086998755	31.0	31.0	34.0	27.0	34.0
4	34.41005536922259	37.0	35.0	37.0	31.0	37.0
5	34.36969698707242	37.0	35.0	37.0	30.0	37.0
6	34.44743836396359	37.0	35.0	37.0	31.0	37.0
7	34.35136807446243	37.0	35.0	37.0	30.0	37.0
8	34.348016948054926	37.0	35.0	37.0	30.0	37.0
9	35.817828478182854	39.0	37.0	39.0	30.0	39.0
10-11	35.75035828433661	39.0	37.0	39.0	30.0	39.0
12-13	35.65692575680715	39.0	37.0	39.0	30.0	39.0
14-15	36.82076634228541	40.0	37.0	41.0	30.0	41.0
16-17	36.80994984862508	40.0	37.0	41.0	30.0	41.0
18-19	36.83781516204672	40.0	37.0	41.0	30.0	41.0
20-21	36.88839683232462	40.0	37.0	41.0	30.0	41.0
22-23	36.850818800711274	40.0	37.0	41.0	30.0	41.0
24-25	36.85292280418669	40.0	37.0	41.0	30.0	41.0
26-27	36.85268206477012	40.0	37.0	41.0	30.0	41.0
28-29	36.833509510176654	40.0	37.0	41.0	30.0	41.0
30-31	36.77918731432287	40.0	37.0	41.0	30.0	41.0
32-33	36.72832029827066	40.0	37.0	41.0	30.0	41.0
34-35	36.64933179844739	40.0	37.0	41.0	30.0	41.0
36-37	36.53575777179354	40.0	36.5	41.0	30.0	41.0
38-39	36.42371317825274	40.0	36.0	41.0	30.0	41.0
40-41	36.29272614495835	40.0	36.0	41.0	29.5	41.0
42-43	36.201185129370245	40.0	36.0	41.0	29.0	41.0
44-45	36.050034082967485	40.0	35.5	41.0	29.0	41.0
46-47	35.926210291066184	40.0	35.0	41.0	28.0	41.0
48-49	35.82218236225827	39.0	35.0	41.0	28.0	41.0
50-51	35.70965586827414	39.0	35.0	41.0	28.0	41.0
52-53	35.58958912906803	39.0	35.0	41.0	28.0	41.0
54-55	35.44395906805936	39.0	35.0	41.0	27.0	41.0
56-57	35.28135194534313	39.0	35.0	41.0	27.0	41.0
58-59	35.09646449502567	39.0	34.5	40.0	26.5	41.0
60-61	34.90070574172363	38.0	34.0	40.0	26.0	41.0
62-63	34.64599441653198	38.0	34.0	40.0	26.0	41.0
64-65	34.40958451496615	38.0	34.0	40.0	26.0	41.0
66-67	34.19094413361468	37.0	34.0	40.0	26.0	41.0
68-69	33.90391605032879	37.0	34.0	40.0	25.0	41.0
70-71	33.59385498093647	36.0	33.0	39.0	24.5	41.0
72-73	33.25400673024773	36.0	33.0	39.0	24.0	41.0
74-75	32.95258436925767	35.5	33.0	39.0	23.5	40.0
76-77	32.62387303597111	35.0	33.0	38.0	23.0	40.0
78-79	32.269610438933306	35.0	32.0	37.0	22.0	39.0
80-81	31.928389847087047	35.0	32.0	37.0	21.5	39.0
82-83	31.615669176315357	35.0	32.0	36.0	20.0	39.0
84-85	31.314349435244722	35.0	32.0	36.0	20.0	37.0
86-87	31.023990125552142	35.0	32.0	35.5	18.5	37.0
88-89	30.774529702269007	35.0	32.0	35.0	17.5	37.0
90-91	30.539290527869447	35.0	31.5	35.0	13.0	36.0
92-93	30.302864267828376	35.0	31.0	35.0	6.5	36.0
94-95	30.064216712357968	34.5	31.0	35.0	2.0	36.0
96-97	29.841509816434296	34.0	31.0	35.0	2.0	35.5
98-99	29.630764085827693	34.0	31.0	35.0	2.0	35.0
100	29.46537635960853	34.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5114358026974912
1101	2	0.5121683072623071
1101	3	0.5561499092859492
1101	4	0.552922247676122
1101	5	0.5412200058033534
1101	6	0.5913157473818487
1101	7	0.6714954676092404
1101	8	0.6238059287823887
1101	9	0.740243655111847
1101	10-11	0.7993227455037584
1101	12-13	0.7589839874922006
1101	14-15	0.9028011649378129
1101	16-17	0.8760775522742748
1101	18-19	0.8371301273387317
1101	20-21	0.8059977770436078
1101	22-23	0.8058547959730475
1101	24-25	0.8266736591646904
1101	26-27	0.8200693296984198
1101	28-29	0.8080167700472316
1101	30-31	0.8130298159477789
1101	32-33	0.7867818908546269
1101	34-35	0.8053621936139521
1101	36-37	0.7694189655768184
1101	38-39	0.7744322288516798
1101	40-41	0.7772173851706086
1101	42-43	0.651066153630147
1101	44-45	0.7024889121713898
1101	46-47	0.6823221872862959
1101	48-49	0.6706426787659581
1101	50-51	0.6788819471866461
1101	52-53	0.6818180402111622
1101	54-55	0.5820684634184232
1101	56-57	0.5861986020097234
1101	58-59	0.5861930713604764
1101	60-61	0.5896440185351963
1101	62-63	0.5533868188194333
1101	64-65	0.6036392236092496
1101	66-67	0.5324536522251293
1101	68-69	0.5245868583929152
1101	70-71	0.5368104741178357
1101	72-73	0.5736890290622796
1101	74-75	0.4783575777282678
1101	76-77	0.5117145908792224
1101	78-79	0.5128203212211702
1101	80-81	0.49950880470614223
1101	82-83	0.4775783124051003
1101	84-85	0.5248154927269333
1101	86-87	0.47144942728255756
1101	88-89	0.43624779003925696
1101	90-91	0.4896465287836982
1101	92-93	0.45577403108093506
1101	94-95	0.533086671127279
1101	96-97	0.49533954338143715
1101	98-99	0.5683727436053907
1101	100	0.5836054037815082
1102	1	0.36199030821605405
1102	2	0.3515912141008606
1102	3	0.42774910642751607
1102	4	0.4418713510816019
1102	5	0.35759802238248284
1102	6	0.4124317076729298
1102	7	0.44736329972231914
1102	8	0.5357471974069696
1102	9	0.6026101705941329
1102	10-11	0.5929151473225147
1102	12-13	0.5831932432846756
1102	14-15	0.7280473716951192
1102	16-17	0.6458502730663866
1102	18-19	0.6176527830665393
1102	20-21	0.6778289734244609
1102	22-23	0.6403791523741447
1102	24-25	0.6369761154296327
1102	26-27	0.5737028416434242
1102	28-29	0.6165494788438295
1102	30-31	0.5513914844719352
1102	32-33	0.5709286568266094
1102	34-35	0.5812042220228264
1102	36-37	0.6098386263894326
1102	38-39	0.6629777538656114
1102	40-41	0.6087101348205763
1102	42-43	0.5729078361168334
1102	44-45	0.5861649753836815
1102	46-47	0.5752076959864283
1102	48-49	0.49375070465587356
1102	50-51	0.5402331544949206
1102	52-53	0.5771773969288176
1102	54-55	0.59305161339568
1102	56-57	0.5173157910917823
1102	58-59	0.5270360518709083
1102	60-61	0.5545393362978857
1102	62-63	0.4392636804921253
1102	64-65	0.47534970064112514
1102	66-67	0.5056286136940145
1102	68-69	0.4761050057748548
1102	70-71	0.46063696514754326
1102	72-73	0.5040511369667939
1102	74-75	0.49884178450201233
1102	76-77	0.4293778025932582
1102	78-79	0.45142068269353786
1102	80-81	0.5063782537003227
1102	82-83	0.47127841769206213
1102	84-85	0.462614104338126
1102	86-87	0.4252214832951253
1102	88-89	0.4285195541988678
1102	90-91	0.4440480532094533
1102	92-93	0.40912944449116395
1102	94-95	0.38104311290384985
1102	96-97	0.42276476383885964
1102	98-99	0.4392373998314838
1102	100	0.3631023830339082
1103	1	0.24290429954575288
1103	2	0.3606851066931398
1103	3	0.3702181632561832
1103	4	0.33289636448512994
1103	5	0.32278216560352035
1103	6	0.320152529395628
1103	7	0.42466390351396655
1103	8	0.46025952175168783
1103	9	0.48556745245024047
1103	10-11	0.5400269022760682
1103	12-13	0.5090193365065119
1103	14-15	0.5842136212032756
1103	16-17	0.5510848405983211
1103	18-19	0.5092145057480693
1103	20-21	0.5206579553084083
1103	22-23	0.5161331728579555
1103	24-25	0.4972938208027813
1103	26-27	0.5511152469845939
1103	28-29	0.49772473834308073
1103	30-31	0.4973341337573487
1103	32-33	0.5443501754178328
1103	34-35	0.4870767012387276
1103	36-37	0.49689558362699415
1103	38-39	0.5255481008272156
1103	40-41	0.5637057542247419
1103	42-43	0.56266465552239
1103	44-45	0.5312954023752852
1103	46-47	0.47684164672586604
1103	48-49	0.44076510424675064
1103	50-51	0.49007239842075734
1103	52-53	0.47124207787878447
1103	54-55	0.3970583772887579
1103	56-57	0.42194284217706723
1103	58-59	0.45868397996125765
1103	60-61	0.4434807766769069
1103	62-63	0.446780493868971
1103	64-65	0.42887419631172463
1103	66-67	0.42551410267550693
1103	68-69	0.4162104751630338
1103	70-71	0.39309609444731564
1103	72-73	0.4302915675365142
1103	74-75	0.41818706950824236
1103	76-77	0.3332942220903874
1103	78-79	0.40156587082617534
1103	80-81	0.36442111968573343
1103	82-83	0.3784733090486867
1103	84-85	0.35800352722698037
1103	86-87	0.3966897106005902
1103	88-89	0.42632683300610097
1103	90-91	0.4086657822257358
1103	92-93	0.37128665495765745
1103	94-95	0.3513608297821733
1103	96-97	0.35938967294796953
1103	98-99	0.43956114110768496
1103	100	0.42295045430617506
1104	1	0.21979198326587834
1104	2	0.23814565192703796
1104	3	0.22615436490059437
1104	4	0.33004544827105775
1104	5	0.27185402987492324
1104	6	0.3128305139331573
1104	7	0.3552095564471003
1104	8	0.3257201689758986
1104	9	0.45698550258831716
1104	10-11	0.40759105858893463
1104	12-13	0.43721935590654937
1104	14-15	0.5138858419895982
1104	16-17	0.4627526958986792
1104	18-19	0.46072370596427703
1104	20-21	0.47889860472144363
1104	22-23	0.48487782597162266
1104	24-25	0.5112371331950598
1104	26-27	0.4461132307619806
1104	28-29	0.41534326328294924
1104	30-31	0.47592342186294445
1104	32-33	0.4385110183718268
1104	34-35	0.4575708025827794
1104	36-37	0.48034601555116296
1104	38-39	0.5359840950389199
1104	40-41	0.6061645040327477
1104	42-43	0.5436697672738902
1104	44-45	0.5261289780495275
1104	46-47	0.5112869660782309
1104	48-49	0.5027421740638331
1104	50-51	0.49181639850935
1104	52-53	0.5018343100316827
1104	54-55	0.4560960276398731
1104	56-57	0.4346538628245611
1104	58-59	0.4641166386387283
1104	60-61	0.4695342487188796
1104	62-63	0.5179963338661366
1104	64-65	0.6045991700126478
1104	66-67	0.5377462542177938
1104	68-69	0.5028319785343527
1104	70-71	0.5020508434940751
1104	72-73	0.5121974478451392
1104	74-75	0.4696471475827835
1104	76-77	0.48575738714905015
1104	78-79	0.4322115932043147
1104	80-81	0.44382250903708
1104	82-83	0.4244465614751789
1104	84-85	0.3567323944010816
1104	86-87	0.38389186147501775
1104	88-89	0.34052514851175175
1104	90-91	0.3715495745917856
1104	92-93	0.3515617157092201
1104	94-95	0.3838040971606347
1104	96-97	0.3821580727138816
1104	98-99	0.38605900105434543
1104	100	0.37473509671222516
1105	1	0.11904644316869195
1105	2	0.20297068015518605
1105	3	0.18058118493469522
1105	4	0.15891809787112265
1105	5	0.1392537605962474
1105	6	0.2132622713729262
1105	7	0.18369057726360438
1105	8	0.2453567904326448
1105	9	0.29156296676300997
1105	10-11	0.29009315674456815
1105	12-13	0.2637511150941094
1105	14-15	0.276599548198071
1105	16-17	0.2451396814152318
1105	18-19	0.300808893044767
1105	20-21	0.2623259649785652
1105	22-23	0.28893475504386856
1105	24-25	0.2565499597845431
1105	26-27	0.3289816337286595
1105	28-29	0.2973930359282946
1105	30-31	0.31182684949976647
1105	32-33	0.24337957229179352
1105	34-35	0.24745693581015615
1105	36-37	0.2757650522890245
1105	38-39	0.30267921066240433
1105	40-41	0.3390673133241151
1105	42-43	0.3241714527647801
1105	44-45	0.28392401415730717
1105	46-47	0.2385128849386362
1105	48-49	0.24629890762797402
1105	50-51	0.2194756401255873
1105	52-53	0.17962987425441668
1105	54-55	0.171976155136619
1105	56-57	0.1610005198040838
1105	58-59	0.10582924350855194
1105	60-61	0.19378617133690312
1105	62-63	0.1951729270238971
1105	64-65	0.19071632324519783
1105	66-67	0.16207070662208167
1105	68-69	0.18877883815257235
1105	70-71	0.23899821573048996
1105	72-73	0.2167744424250202
1105	74-75	0.20306376111754076
1105	76-77	0.12956635556028573
1105	78-79	0.15709135614274317
1105	80-81	0.14902639901301384
1105	82-83	0.11805341447840689
1105	84-85	0.06686644345443682
1105	86-87	0.09373083997973453
1105	88-89	0.11645713423872905
1105	90-91	0.12878054951429263
1105	92-93	0.09704713110167873
1105	94-95	0.020761940462175943
1105	96-97	0.07604223168801028
1105	98-99	0.032671709890610856
1105	100	-0.08666454165414805
1106	1	0.19630185874483175
1106	2	0.21244681248058228
1106	3	0.23949800546714073
1106	4	0.2777216675412788
1106	5	0.26085584721622723
1106	6	0.2740207418646321
1106	7	0.30606567513299865
1106	8	0.2843676347282553
1106	9	0.3306000600013519
1106	10-11	0.39292266077242743
1106	12-13	0.4208859479552842
1106	14-15	0.4851459210976756
1106	16-17	0.43518110911081465
1106	18-19	0.3925508336042114
1106	20-21	0.43961195277876897
1106	22-23	0.398532262329617
1106	24-25	0.45070450755026314
1106	26-27	0.3777246821116549
1106	28-29	0.42625087465899014
1106	30-31	0.4351481852979049
1106	32-33	0.42268514469468244
1106	34-35	0.40196133672574064
1106	36-37	0.4579373914071141
1106	38-39	0.468885718361129
1106	40-41	0.5461809188614097
1106	42-43	0.4551884870337659
1106	44-45	0.42046746286433745
1106	46-47	0.41380481422724813
1106	48-49	0.3886428886831794
1106	50-51	0.3665812208783521
1106	52-53	0.4061931040792146
1106	54-55	0.3255749926705107
1106	56-57	0.2943320935698708
1106	58-59	0.29775207171748264
1106	60-61	0.3023442619757404
1106	62-63	0.3770729019327774
1106	64-65	0.3417840423620504
1106	66-67	0.3868173537250783
1106	68-69	0.3657454889588294
1106	70-71	0.3450762212774947
1106	72-73	0.3736980140397037
1106	74-75	0.3564870777038962
1106	76-77	0.30482248169329296
1106	78-79	0.29496609641429217
1106	80-81	0.35881469387276255
1106	82-83	0.3040100830304837
1106	84-85	0.2646808141748167
1106	86-87	0.28770769824302533
1106	88-89	0.32900921774164615
1106	90-91	0.32052178482197036
1106	92-93	0.26442356473803486
1106	94-95	0.3432050090133636
1106	96-97	0.36900401643307745
1106	98-99	0.3142847554441808
1106	100	0.28467423525901836
1107	1	0.11916010054502735
1107	2	0.17921663565986634
1107	3	0.1848374162465376
1107	4	0.16205046391864641
1107	5	0.15566133795635295
1107	6	0.17179219485437613
1107	7	0.09289555513680625
1107	8	0.09938126707619688
1107	9	0.16444929850075596
1107	10-11	0.21549728019500236
1107	12-13	0.1584366452070114
1107	14-15	0.2633922622630749
1107	16-17	0.23896346866548868
1107	18-19	0.21969775338946107
1107	20-21	0.24898481205411116
1107	22-23	0.24079368361250175
1107	24-25	0.2177565039399525
1107	26-27	0.35149888709692334
1107	28-29	0.27408188572432834
1107	30-31	0.24750206246879713
1107	32-33	0.22924181965086632
1107	34-35	0.23143675069654535
1107	36-37	0.1797438676054668
1107	38-39	0.18346202097549025
1107	40-41	0.26739690845762
1107	42-43	0.25937120048929074
1107	44-45	0.2352222251502809
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2216	90-91	-1.3813421142808124
2216	92-93	-1.3887914665909342
2216	94-95	-1.309509280500233
2216	96-97	-1.3515726558200392
2216	98-99	-1.422925182421995
2216	100	-1.4990572244102545
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	111046.0
3	21848.0
4	13280.0
5	10526.0
6	9614.0
7	9727.0
8	11218.0
9	12493.0
10	14197.0
11	15397.0
12	16910.0
13	18476.0
14	20607.0
15	20967.0
16	21309.0
17	21428.0
18	20644.0
19	19877.0
20	19754.0
21	21210.0
22	23743.0
23	28723.0
24	35561.0
25	44744.0
26	54647.0
27	61072.0
28	72318.0
29	91619.0
30	109086.0
31	124332.0
32	158037.0
33	207678.0
34	283045.0
35	406184.0
36	670227.0
37	1145488.0
38	1470320.0
39	511409.0
40	887.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.15087508246539	21.220570467255776	15.20659257312775	22.421961877151084
2	15.001206795905428	20.933852573224513	40.79499767495716	23.269942955912903
3	18.03785725418018	26.046740157862814	31.159048235259984	24.75635435269702
4	12.413209070530222	17.73428343543237	39.5369778650768	30.315529628960608
5	12.757312835333954	38.4128184007054	35.52067374085116	13.309195023109496
6	29.252259654889073	38.72101981642117	18.47364849093237	13.553072037757385
7	25.822578212949193	33.51297985626704	23.094212459353084	17.570229471430686
8	24.3033280835362	36.40939512419891	21.226597278043716	18.06067951422117
9	24.667315554744494	17.44676315208487	22.522055139070453	35.36386615410018
10-11	23.109149018600487	27.710104907613193	29.488511504013122	19.692234569773202
12-13	23.55212700364974	25.54568401952208	29.342126269096592	21.56006270773159
14-15	21.19028835478766	27.863614303598332	26.608104900138173	24.33799244147583
16-17	20.48641257025418	31.06314938603885	27.45924530068094	20.991192743026023
18-19	20.477701323588093	29.958960639791027	29.326259962160066	20.23707807446081
20-21	21.917247237194925	29.000910449729783	28.707485219106694	20.374357093968598
22-23	21.53443626650025	28.94279070061626	28.83855529388945	20.684217738994036
24-25	21.2385481723966	28.969328768907026	28.745942650961048	21.046180407735328
26-27	20.880299499591608	29.187861348203526	28.757350923548707	21.17448822865616
28-29	20.868530292379038	29.173972544516662	28.980635235256575	20.97686192784772
30-31	21.246730190577637	29.45523261006162	28.509795028835548	20.788242170525194
32-33	20.876589712246325	29.280921513406483	28.92361496169005	20.91887381265715
34-35	21.24666847973892	28.96179688109408	28.768860694912647	21.022673944254354
36-37	21.36681256549783	29.072667501362137	28.526033888568573	21.03448604457146
38-39	21.089007664168168	29.21343074311859	28.678579335209843	21.0189822575034
40-41	21.082686002033938	28.904482767315688	28.83867790844953	21.174153322200844
42-43	21.02031782863173	28.918792859375753	28.801745057913784	21.259144254078734
44-45	21.183626928497272	29.12813748468208	28.627590551765493	21.060645035055153
46-47	21.258196690894934	29.26526478710362	28.43690901006846	21.03962951193298
48-49	21.09941275447257	28.954246530447563	28.573373136926435	21.372967578153432
50-51	21.091660059392105	29.028215381465326	28.444987418771	21.43513714037157
52-53	21.05814855921175	29.06929568219332	28.501819891117712	21.370735867477215
54-55	21.18955336406369	29.335561368799745	28.392441208367696	21.08244405876886
56-57	21.16592543963371	29.023525172987647	28.296767894788154	21.513781492590482
58-59	21.089954977103194	28.798067505590865	28.630820088275	21.481157429030944
60-61	21.261529862747178	29.068527242022927	28.250223425216937	21.419719470012954
62-63	21.092102126153765	29.024502325890488	28.40027867338179	21.483116874573955
64-65	21.11103342290156	28.875526704272445	28.463567401911067	21.549872470914927
66-67	20.896198056250388	28.942250094726653	28.42908589390463	21.73246595511833
68-69	21.203657999835887	29.17880536564439	28.22819396314828	21.38934267137144
70-71	21.139720983458833	28.849575898433567	28.255320900397912	21.755382217709688
72-73	20.979421912901085	28.88137261128152	28.582787808617958	21.556417667199433
74-75	20.984547638610174	28.99113752448141	28.39052816076434	21.633786676144084
76-77	21.054930676246762	28.964958512678272	28.190734834061253	21.789375977013712
78-79	21.03093856333365	28.798093177047928	28.61157851515034	21.55938974446808
80-81	20.997251256045768	28.837935432854643	28.46850127670858	21.69631203439101
82-83	20.814962658205804	28.84241745139714	28.750520855602257	21.5920990347948
84-85	20.81108750779008	28.709364034329482	28.534512056387108	21.945036401493333
86-87	21.02793996402591	28.808348255067877	28.387346189189156	21.776365591717052
88-89	20.873935794341378	28.636144405173646	28.561010859491837	21.92890894099314
90-91	20.83144107631832	28.577352472896344	28.54256446092117	22.048641989864166
92-93	20.965438135436283	28.629844123838755	28.443301062505295	21.96141667821967
94-95	20.78559533855316	28.486517430246884	28.669092226542187	22.058795004657767
96-97	20.822442797648428	28.603499156207494	28.494178459985697	22.07987958615838
98-99	20.838034035302954	28.48342044354612	28.489466913832658	22.18907860731827
100	20.905873570937782	28.55565926009379	28.315274239286737	22.22319292968169
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3312.0
1	2549.0
2	2157.5
3	2484.5
4	2321.0
5	1993.0
6	1676.0
7	1578.0
8	1627.0
9	1703.5
10	1598.0
11	1402.0
12	1376.5
13	1456.0
14	1585.0
15	1704.5
16	1987.5
17	2490.0
18	3253.5
19	4265.0
20	5830.5
21	8240.5
22	12658.0
23	20428.5
24	28611.0
25	37239.5
26	50279.0
27	67478.0
28	90951.5
29	111774.5
30	122894.5
31	136949.5
32	155113.5
33	170064.5
34	184373.5
35	197146.0
36	205103.5
37	210718.5
38	214298.0
39	215681.5
40	218459.0
41	215937.0
42	206508.5
43	199829.0
44	195288.5
45	191252.5
46	187488.0
47	182076.5
48	177921.0
49	175917.5
50	172669.5
51	164822.0
52	154012.0
53	145770.5
54	141951.5
55	137897.5
56	124209.5
57	107679.5
58	94879.5
59	83499.5
60	71755.5
61	59656.5
62	50438.0
63	41501.5
64	32901.0
65	26084.5
66	20540.0
67	16023.0
68	12105.5
69	9056.5
70	6782.5
71	5138.0
72	3866.5
73	2755.5
74	1911.5
75	1309.0
76	873.0
77	613.0
78	409.0
79	279.5
80	209.5
81	144.5
82	102.5
83	79.0
84	62.0
85	42.5
86	34.5
87	33.0
88	25.0
89	18.5
90	16.5
91	14.0
92	13.0
93	10.0
94	7.5
95	6.5
96	5.5
97	4.5
98	8.5
99	14.5
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07479364711025005
2	0.01222669541261134
3	0.0161223735371813
4	0.010135508886868158
5	0.008162373213384673
6	0.007150508765444424
7	0.019461526215384117
8	0.00893813595680553
9	0.008955000364271201
10-11	0.012091780152885971
12-13	0.014731059921263454
14-15	0.009789788533821906
16-17	0.012319449653672529
18-19	0.007791356249139915
20-21	0.011973729300626276
22-23	0.012825381877642653
24-25	0.01517796671910373
26-27	0.004907542572510206
28-29	0.010658305518303953
30-31	0.010025890238341297
32-33	0.011897839467030758
34-35	0.00818766982458318
36-37	0.009098347827729404
38-39	0.018306314303985667
40-41	0.012724195432848627
42-43	0.007529957933422018
44-45	0.019107373658605032
46-47	0.017471526134434964
48-49	0.017496822745633467
50-51	0.009730763107692058
52-53	0.00985724616368459
54-55	0.011096780112411395
56-57	0.020068644884148265
58-59	0.001846652617490954
60-61	0.006256695169763872
62-63	0.014225127697293331
64-65	0.023483687395946605
66-67	0.012589280173123262
68-69	0.013145805619490396
70-71	0.024773814567070424
72-73	0.019925297420690064
74-75	0.01971449232736918
76-77	0.00923326308745477
78-79	0.01051495805484575
80-81	0.01892186517648265
82-83	0.011830381837168075
84-85	0.010675169925769624
86-87	0.01578508538786788
88-89	0.014697331106332112
90-91	0.014865975180988821
92-93	0.007656440989414549
94-95	0.014250424308491836
96-97	0.012842246285108322
98-99	0.009595847847966691
100	0.006914407060925032
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5929648.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.640420066583054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.52708765612329	43.70299726494127
2	14.96603495721272	17.552291532442403
3	4.910230977294684	8.638140213975266
4	2.1266133645962104	4.988220040765253
5	1.0507163401155044	3.0807223777597965
6	0.6176709682809792	2.1732291025757835
7	0.37686976879618467	1.5469861086823011
8	0.27851261465456745	1.3065677373748967
9	0.19701125128832353	1.03975402800514
>10	0.8731903198580984	8.889556209934932
>50	0.04739061959341494	1.9034337086118878
>100	0.024463340899819254	2.8839288069320217
>500	0.0031414699739752343	1.2929455312775056
>1k	0.0010663513121656446	1.001227336721422
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.6864407465670813E-5	0.0	0.0	0.0
3	0.0	1.6864407465670813E-5	0.0	0.0	0.0
4	0.0	1.6864407465670813E-5	0.0	0.0	0.0
5	0.0	1.6864407465670813E-5	0.0	0.0	0.0
6	0.0	1.6864407465670813E-5	0.0	0.0	0.0
7	0.0	1.6864407465670813E-5	0.0	0.0	0.0
8	0.0	1.6864407465670813E-5	0.0	0.0	0.0
9	0.0	1.6864407465670813E-5	0.0	3.3728814931341626E-5	0.0
10-11	0.0	2.5296611198506218E-5	0.0	4.2161018664177034E-5	0.0
12-13	0.0	3.3728814931341626E-5	0.0	5.9025426129847844E-5	0.0
14-15	0.0	3.3728814931341626E-5	0.0	6.745762986268325E-5	0.0
16-17	0.0	3.3728814931341626E-5	0.0	8.432203732835405E-5	0.0
18-19	0.0	6.745762986268324E-5	0.0	9.275424106118947E-5	0.0
20-21	0.0	8.432203732835405E-5	0.0	1.602118709238727E-4	0.0
22-23	0.0	1.0118644479402487E-4	0.0	3.2042374184774547E-4	0.0
24-25	0.0	1.0118644479402487E-4	0.0	7.083051135581742E-4	0.0
26-27	0.0	1.0118644479402487E-4	0.0	0.001433474634582019	0.0
28-29	0.0	1.0961864852686027E-4	0.0	0.004047457791760994	0.0
30-31	0.0	1.349152597253665E-4	0.0	0.010894407222823345	0.0
32-33	0.0	1.517796671910373E-4	0.0	0.02674695024055391	0.0
34-35	0.0	1.517796671910373E-4	0.0	0.05167254447481537	0.0
36-37	0.0	1.7707627838954354E-4	0.0	0.09362275804567152	0.0
38-39	0.0	1.9394068585521434E-4	0.0	0.14810322636352108	0.0
40-41	0.0	2.529661119850622E-4	0.0	0.2037220421853034	0.0
42-43	0.0	2.529661119850622E-4	0.0	0.26086708688272897	0.0
44-45	0.0	2.613983157178976E-4	0.0	0.33574505603030735	0.0
46-47	0.0	3.541525567790871E-4	0.0	0.428288491998176	0.0
48-49	0.0	3.710169642447579E-4	0.0	0.5254106145929742	0.0
50-51	0.0	4.5533900157311195E-4	0.0	0.6236457880805066	0.0
52-53	0.0	4.890678165044536E-4	0.0	0.72209176666136	0.0
54-55	0.0	4.890678165044536E-4	0.0	0.8358421950173096	0.0
56-57	0.0	4.890678165044536E-4	0.0	0.9734641921409164	0.0
58-59	0.0	4.890678165044536E-4	0.0	1.1282541560645758	0.0
60-61	0.0	4.97500020237289E-4	0.0	1.2886683998780366	0.0
62-63	0.0	5.059322239701244E-4	0.0	1.4512328556433705	0.0
64-65	0.0	5.227966314357952E-4	0.0	1.6293462950920528	0.0
66-67	0.0	5.227966314357952E-4	0.0	1.8387179137783558	0.0
68-69	0.0	5.227966314357952E-4	0.0	2.073748728423677	0.0
70-71	0.0	5.312288351686306E-4	0.0	2.3253909844226843	0.0
72-73	0.0	5.39661038901466E-4	0.0	2.589850190095601	0.0
74-75	0.0	5.39661038901466E-4	0.0	2.8615948197936873	0.0
76-77	0.0	5.480932426343015E-4	0.0	3.150296611198506	0.0
78-79	0.0	5.565254463671368E-4	0.0	3.482011073844518	0.0
80-81	0.0	5.902542612984784E-4	0.0	3.8502454108574407	0.0
82-83	0.0	6.071186687641492E-4	0.0	4.221776739529901	0.0
84-85	0.0	6.2398307622982E-4	0.0	4.5997924328729125	0.0
86-87	0.0	6.2398307622982E-4	0.0	5.000372703404992	0.0
88	0.0	6.2398307622982E-4	0.0	5.323587504688305	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7515	0.0	49.372795	1
GTATCAA	12700	0.0	36.176834	1
ATCAACG	14145	0.0	31.764748	3
TCAACGC	14130	0.0	31.631361	4
TATCAAC	15670	0.0	29.003336	2
CAACGCA	15555	0.0	28.824484	5
AACGCAG	17395	0.0	25.93914	6
GTACATG	22570	0.0	24.836155	1
TACATGG	22620	0.0	24.2476	2
CGCAGAG	18445	0.0	23.593761	8
ACGCAGA	19445	0.0	22.793966	7
GTGGTAT	4120	0.0	22.599962	1
ACATGGG	23755	0.0	22.416374	3
GCAGAGT	20500	0.0	20.403843	9
TGGTATC	4310	0.0	20.064625	2
GTCGTAC	290	2.7685019E-9	19.459108	1
CATGGGG	14230	0.0	19.387093	4
CATGGGT	7330	0.0	19.042852	4
CAGAGTA	19925	0.0	18.421963	10-11
AGAGTAC	18205	0.0	17.180689	10-11
>>END_MODULE