FastQCFastQC Report
Wed 25 May 2016
SRR2049451_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049451_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5929648
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234640.3957064567744999No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231880.3910518803139748No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127830.21557772063367No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71840.12115390323337913No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144100.059.9059561
GTATCAA252050.040.2247771
GTGGTAT51900.038.3625681
TGGTATC56350.034.2012372
ATCAACG319750.031.4009383
TCAACGC323700.031.0903684
TATCAAC327350.030.7149892
CAACGCA338950.029.6360825
AACGCAG359750.028.0620546
CGCAGAG397550.025.0628268
ACGCAGA404600.024.8816727
GTACATG222050.023.4231971
GCAGAGT421900.023.3935289
GAGTACT287850.022.94830112-13
TACATGG226900.022.2495732
CAGAGTA415750.021.7612810-11
CATGGGT68200.021.504144
ACATGGG231000.021.1628073
GTACTTT314000.020.89496814-15
AGAGTAC400200.020.4870710-11
Produced by FastQC (version 0.11.5)