##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049451_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5929648
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.828246634538846	31.0	31.0	34.0	30.0	34.0
2	31.90349494607437	33.0	31.0	34.0	30.0	34.0
3	31.97289754805007	33.0	31.0	34.0	30.0	34.0
4	35.740186095363505	37.0	35.0	37.0	33.0	37.0
5	35.839384732449545	37.0	35.0	37.0	35.0	37.0
6	35.94134322981735	37.0	35.0	37.0	35.0	37.0
7	35.90063693494116	37.0	35.0	37.0	35.0	37.0
8	35.913440561733175	37.0	35.0	37.0	35.0	37.0
9	37.620059065900705	39.0	37.0	39.0	35.0	39.0
10-11	37.6052883746219	39.0	37.0	39.0	35.0	39.0
12-13	37.60162660582888	39.0	37.0	39.0	35.0	39.0
14-15	38.88008074003719	40.0	38.0	41.0	35.0	41.0
16-17	38.89333177955926	40.0	38.0	41.0	35.0	41.0
18-19	38.989114699557206	40.0	38.5	41.0	35.5	41.0
20-21	39.02246845006651	40.0	39.0	41.0	35.0	41.0
22-23	38.996341351122354	40.0	39.0	41.0	35.0	41.0
24-25	38.98109634838357	40.0	38.5	41.0	35.0	41.0
26-27	38.88330867194815	40.0	38.0	41.0	35.0	41.0
28-29	38.79848205154842	40.0	38.0	41.0	35.0	41.0
30-31	38.671240687474196	40.0	38.0	41.0	35.0	41.0
32-33	38.51754775325618	40.0	38.0	41.0	34.5	41.0
34-35	38.389002686162826	40.0	38.0	41.0	34.0	41.0
36-37	38.244627758679776	40.0	38.0	41.0	34.0	41.0
38-39	38.13046103242553	40.0	38.0	41.0	34.0	41.0
40-41	38.027956296899916	40.0	38.0	41.0	33.5	41.0
42-43	37.86615917167427	40.0	38.0	41.0	33.0	41.0
44-45	37.66463312830711	40.0	37.0	41.0	33.0	41.0
46-47	37.36448091016533	40.0	37.0	41.0	32.5	41.0
48-49	37.071931925807405	40.0	37.0	41.0	31.5	41.0
50-51	36.89465799656236	40.0	37.0	41.0	31.0	41.0
52-53	36.7816981716284	40.0	36.5	41.0	31.0	41.0
54-55	36.64366333381003	40.0	36.0	41.0	31.0	41.0
56-57	36.501761656003865	39.5	36.0	41.0	31.0	41.0
58-59	36.330260666400434	39.0	35.0	41.0	30.5	41.0
60-61	36.149856028553465	39.0	35.0	41.0	30.0	41.0
62-63	35.93993361831933	39.0	35.0	41.0	30.0	41.0
64-65	35.73137165983546	38.5	35.0	40.5	30.0	41.0
66-67	35.47657019438591	38.0	35.0	40.0	29.0	41.0
68-69	35.19082810649131	37.0	35.0	40.0	29.0	41.0
70-71	34.9116350582699	37.0	35.0	40.0	29.0	41.0
72-73	34.5820223224043	36.5	34.0	39.0	29.0	41.0
74-75	34.259328884277785	36.0	34.0	39.0	28.5	41.0
76-77	33.93966817254582	35.5	34.0	39.0	28.0	40.0
78-79	33.62694193651967	35.0	34.0	37.5	28.0	39.5
80-81	33.313696951319876	35.0	34.0	37.0	28.0	39.0
82-83	33.03331681745696	35.0	34.0	36.5	28.0	39.0
84-85	32.741320479731684	35.0	34.0	36.0	27.0	37.5
86-87	32.48787347916773	35.0	34.0	36.0	27.0	37.0
88-89	32.26265294331131	35.0	33.0	35.5	26.5	37.0
90-91	32.066491467958976	35.0	33.0	35.0	26.0	36.0
92-93	31.896799439022352	35.0	33.0	35.0	26.0	36.0
94-95	31.732376019622073	35.0	33.0	35.0	26.0	36.0
96-97	31.585529613224935	35.0	33.0	35.0	25.0	36.0
98-99	31.447946151272387	35.0	33.0	35.0	25.0	35.5
100	31.33799308154548	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.29447245492153584
1101	2	0.32583596924395763
1101	3	0.4719696685116581
1101	4	0.28906743073803653
1101	5	0.24656415532379583
1101	6	0.23633742743422914
1101	7	0.26259200528823357
1101	8	0.26205559238594134
1101	9	0.33729328453804897
1101	10-11	0.32037460586935396
1101	12-13	0.2893359395272057
1101	14-15	0.46434173529234357
1101	16-17	0.4339886198194378
1101	18-19	0.4305593956026712
1101	20-21	0.437748093292349
1101	22-23	0.4579643018047648
1101	24-25	0.42125580608227864
1101	26-27	0.40616614643052174
1101	28-29	0.431040911731813
1101	30-31	0.471733531652589
1101	32-33	0.5194872263587555
1101	34-35	0.5245685118120633
1101	36-37	0.5189300447617953
1101	38-39	0.46517191781390466
1101	40-41	0.46667370477261727
1101	42-43	0.424647022897787
1101	44-45	0.45286083485481754
1101	46-47	0.4900524066238674
1101	48-49	0.5287815439266268
1101	50-51	0.4729343750847619
1101	52-53	0.4867679065719912
1101	54-55	0.48835236231933976
1101	56-57	0.4833816422696131
1101	58-59	0.45357487304618616
1101	60-61	0.3894265610934582
1101	62-63	0.4169644878607244
1101	64-65	0.4100284991051879
1101	66-67	0.42232299560513553
1101	68-69	0.4031712574866404
1101	70-71	0.4228227417845005
1101	72-73	0.4623722672263071
1101	74-75	0.41247447335386056
1101	76-77	0.4146407799581908
1101	78-79	0.4166703180950364
1101	80-81	0.3925622388720029
1101	82-83	0.34789904911122704
1101	84-85	0.3582987609663917
1101	86-87	0.3650148822991568
1101	88-89	0.3617177571958834
1101	90-91	0.4060378993163525
1101	92-93	0.42717990887019397
1101	94-95	0.4102748738114492
1101	96-97	0.3386523487170514
1101	98-99	0.47049642317444196
1101	100	0.4858774842996745
1102	1	0.2690718382545434
1102	2	0.293478849119186
1102	3	0.3698675901754491
1102	4	0.2544422881040447
1102	5	0.25715898862627995
1102	6	0.21228700072840923
1102	7	0.23531317799704254
1102	8	0.24058600429793842
1102	9	0.2829200845295432
1102	10-11	0.31012685768620685
1102	12-13	0.29682936694541695
1102	14-15	0.37559046481672453
1102	16-17	0.37428989622757314
1102	18-19	0.37497487894887627
1102	20-21	0.40942252065400453
1102	22-23	0.3351727273477678
1102	24-25	0.35425218749222864
1102	26-27	0.29984145841292786
1102	28-29	0.3877734764928391
1102	30-31	0.36082104017680194
1102	32-33	0.4261983627934569
1102	34-35	0.40859734647358437
1102	36-37	0.42203051274145764
1102	38-39	0.3563603326711302
1102	40-41	0.44504621342007056
1102	42-43	0.36362503823242776
1102	44-45	0.3935193456882331
1102	46-47	0.4253490708369796
1102	48-49	0.43019773307247533
1102	50-51	0.44327933398490416
1102	52-53	0.4222198687590577
1102	54-55	0.38614540027001
1102	56-57	0.3701789040231134
1102	58-59	0.38052269099373603
1102	60-61	0.3394687471669684
1102	62-63	0.34881920072718486
1102	64-65	0.3536233763275831
1102	66-67	0.3417971787914027
1102	68-69	0.3808347570664168
1102	70-71	0.4228814929752289
1102	72-73	0.39675809919391725
1102	74-75	0.36717089401987124
1102	76-77	0.3580305717258412
1102	78-79	0.3274940907418795
1102	80-81	0.348968719421336
1102	82-83	0.3347191634165796
1102	84-85	0.25534666315318333
1102	86-87	0.2908634941640855
1102	88-89	0.25648709488600474
1102	90-91	0.2899204978842391
1102	92-93	0.3299281340103093
1102	94-95	0.33549157810707086
1102	96-97	0.3459540267477159
1102	98-99	0.39443535703576416
1102	100	0.3856448114606188
1103	1	0.24009040013052996
1103	2	0.2557400160431875
1103	3	0.3659464535560879
1103	4	0.20271438321963586
1103	5	0.2246373215032449
1103	6	0.17157399332137402
1103	7	0.19803206397384088
1103	8	0.16439567877048944
1103	9	0.2595038727008401
1103	10-11	0.22865844629792065
1103	12-13	0.20595256178327048
1103	14-15	0.2887417440772637
1103	16-17	0.3044761923546986
1103	18-19	0.3212029357016064
1103	20-21	0.33052857220410203
1103	22-23	0.2857256804963839
1103	24-25	0.31261450907570776
1103	26-27	0.34690316585010805
1103	28-29	0.36417458403862213
1103	30-31	0.3490183689049928
1103	32-33	0.4032244027463321
1103	34-35	0.38923816177639026
1103	36-37	0.36747756937224096
1103	38-39	0.31436669848510945
1103	40-41	0.3407498210981643
1103	42-43	0.37529885872176294
1103	44-45	0.3635920023139505
1103	46-47	0.3812721081114461
1103	48-49	0.4355868223112225
1103	50-51	0.4390468744888736
1103	52-53	0.36610250186819115
1103	54-55	0.3589223485565398
1103	56-57	0.3574660366915978
1103	58-59	0.30700422691174367
1103	60-61	0.264095490488792
1103	62-63	0.2518212823011865
1103	64-65	0.27165718630119073
1103	66-67	0.3227915050137753
1103	68-69	0.30502538949444613
1103	70-71	0.27931953069840176
1103	72-73	0.31112728045106053
1103	74-75	0.33964896659222177
1103	76-77	0.30493062424697825
1103	78-79	0.26850361530197375
1103	80-81	0.2463842110260117
1103	82-83	0.2567051955574371
1103	84-85	0.2552489985888968
1103	86-87	0.1953675549160181
1103	88-89	0.2672745781747423
1103	90-91	0.27333050123224467
1103	92-93	0.27591179924881004
1103	94-95	0.2801441232246127
1103	96-97	0.27363817715948713
1103	98-99	0.3232615975238602
1103	100	0.2787799604641563
1104	1	0.22189452254870545
1104	2	0.24960235961503585
1104	3	0.3367435659178959
1104	4	0.19949356757197023
1104	5	0.20616396465972997
1104	6	0.1716368144293483
1104	7	0.20719619392781397
1104	8	0.20928977530212478
1104	9	0.2623618434724122
1104	10-11	0.2687837986754644
1104	12-13	0.24221957705809416
1104	14-15	0.3653493577631295
1104	16-17	0.3487427818951758
1104	18-19	0.2767285785054341
1104	20-21	0.3059769621346504
1104	22-23	0.3481662798576082
1104	24-25	0.33799258243094243
1104	26-27	0.33287778954039027
1104	28-29	0.3495111394089392
1104	30-31	0.34176285905291337
1104	32-33	0.38089817061351994
1104	34-35	0.3705089268356332
1104	36-37	0.37063882459403175
1104	38-39	0.39119979141434413
1104	40-41	0.378911326852446
1104	42-43	0.37158464103356437
1104	44-45	0.40126524655797624
1104	46-47	0.3573645887119312
1104	48-49	0.3781892061911236
1104	50-51	0.37392524933757443
1104	52-53	0.4108888523384735
1104	54-55	0.3947626382965197
1104	56-57	0.39345445846745264
1104	58-59	0.39238498947522515
1104	60-61	0.39275516352781636
1104	62-63	0.43341211372795385
1104	64-65	0.3729871481211333
1104	66-67	0.40970920409875333
1104	68-69	0.4222012819660321
1104	70-71	0.37136248885209255
1104	72-73	0.4450796352548352
1104	74-75	0.43327965818289726
1104	76-77	0.3767862825318673
1104	78-79	0.41195224530623165
1104	80-81	0.37650985802702763
1104	82-83	0.3947767010170935
1104	84-85	0.3470268297923411
1104	86-87	0.3238741878064815
1104	88-89	0.3643786276343022
1104	90-91	0.3770872513397876
1104	92-93	0.3690646334343697
1104	94-95	0.359756677629985
1104	96-97	0.3613112949410002
1104	98-99	0.40134392133884944
1104	100	0.3822467764614039
1105	1	0.19378672469281
1105	2	0.19438775810703746
1105	3	0.29173327412773276
1105	4	0.19168571633502296
1105	5	0.21716311867221805
1105	6	0.13632106847980907
1105	7	0.1517043711662751
1105	8	0.10067846336382047
1105	9	0.20445536754873928
1105	10-11	0.19016821737344713
1105	12-13	0.19197175207519024
1105	14-15	0.23393219241861374
1105	16-17	0.2625463853518042
1105	18-19	0.22939925989715704
1105	20-21	0.23588227198257528
1105	22-23	0.22183533599249472
1105	24-25	0.25417426721906367
1105	26-27	0.2079255750163398
1105	28-29	0.27559424330269167
1105	30-31	0.30055173661299506
1105	32-33	0.30576114908883056
1105	34-35	0.2974065946176694
1105	36-37	0.28436480088628
1105	38-39	0.22374596149325043
1105	40-41	0.24715045016388615
1105	42-43	0.25439071809358893
1105	44-45	0.19309458738175067
1105	46-47	0.1822746165997131
1105	48-49	0.2682069980087576
1105	50-51	0.23505094957376116
1105	52-53	0.22245203234594868
1105	54-55	0.27597434776254204
1105	56-57	0.22763985723836555
1105	58-59	0.28912861595072314
1105	60-61	0.2224561989785272
1105	62-63	0.26742487807885595
1105	64-65	0.24648792952143594
1105	66-67	0.24241118242755277
1105	68-69	0.2374062916447386
1105	70-71	0.2784377291636204
1105	72-73	0.2722065925141095
1105	74-75	0.28463542064893943
1105	76-77	0.25460976698823146
1105	78-79	0.2563915329980162
1105	80-81	0.231156225042497
1105	82-83	0.19540018556209304
1105	84-85	0.14765719599327554
1105	86-87	0.13975726868057592
1105	88-89	0.10832291703222552
1105	90-91	0.15433556881726673
1105	92-93	0.14820843657032157
1105	94-95	0.09965042825094628
1105	96-97	0.11510242591756281
1105	98-99	0.20135448233469688
1105	100	0.18253314458263503
1106	1	0.20228896844313127
1106	2	0.21269845736340542
1106	3	0.26969998953806495
1106	4	0.1858048217423871
1106	5	0.1608792254949094
1106	6	0.18426927549410266
1106	7	0.1963638169683719
1106	8	0.1350068759297116
1106	9	0.1630994165607973
1106	10-11	0.18104999089080565
1106	12-13	0.17459365085154843
1106	14-15	0.2537200295561419
1106	16-17	0.30948405207045226
1106	18-19	0.2748224895904485
1106	20-21	0.22189274206732534
1106	22-23	0.24116700975066863
1106	24-25	0.2578524694621649
1106	26-27	0.25121220614192197
1106	28-29	0.2819021546397522
1106	30-31	0.3095425714954416
1106	32-33	0.280290114137145
1106	34-35	0.25811435989595566
1106	36-37	0.3087798564601911
1106	38-39	0.3132472568496567
1106	40-41	0.3162399365685076
1106	42-43	0.30437048390500365
1106	44-45	0.31057342772307095
1106	46-47	0.35444699481463715
1106	48-49	0.33849670726286973
1106	50-51	0.27839844480674003
1106	52-53	0.29211159726142455
1106	54-55	0.35224227258159857
1106	56-57	0.32153707736596004
1106	58-59	0.2715460922855968
1106	60-61	0.2770419565310007
1106	62-63	0.2554393757420428
1106	64-65	0.26042816095539933
1106	66-67	0.27523396418666835
1106	68-69	0.29018229270906915
1106	70-71	0.3231277421612546
1106	72-73	0.32781481427131354
1106	74-75	0.2787296913884987
1106	76-77	0.29879974265528375
1106	78-79	0.28776536180394174
1106	80-81	0.24389407135772956
1106	82-83	0.24785935761336475
1106	84-85	0.2522930056784318
1106	86-87	0.24682506553855177
1106	88-89	0.1746171173195279
1106	90-91	0.24609736579675712
1106	92-93	0.28399331848923026
1106	94-95	0.23772493957087448
1106	96-97	0.2835837225590154
1106	98-99	0.26609129563837897
1106	100	0.34385728607594856
1107	1	0.09906244543642373
1107	2	0.0671076271051021
1107	3	0.19668300017340457
1107	4	0.1293173500382352
1107	5	0.14246298283634218
1107	6	0.11717182772769519
1107	7	0.08633036425488427
1107	8	0.10125642110330801
1107	9	0.11540506735911293
1107	10-11	0.11289110169537508
1107	12-13	0.11083920014881699
1107	14-15	0.18458080693309142
1107	16-17	0.13280027239811432
1107	18-19	0.1310819222039754
1107	20-21	0.1691006345213637
1107	22-23	0.1948937271689175
1107	24-25	0.17809135989735125
1107	26-27	0.18442491939417494
1107	28-29	0.20355996001256926
1107	30-31	0.182898591202445
1107	32-33	0.15589454989334683
1107	34-35	0.12577671560481463
1107	36-37	0.14392351270124237
1107	38-39	0.09314155370967114
1107	40-41	0.1443483947445685
1107	42-43	0.1678753419132164
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2216	74-75	-0.9099576936001554
2216	76-77	-0.7807615207635266
2216	78-79	-0.827350311856911
2216	80-81	-0.794900974156306
2216	82-83	-0.8275205088954323
2216	84-85	-0.9185683920167804
2216	86-87	-0.8400275313461805
2216	88-89	-0.823011071781508
2216	90-91	-0.8062413022068782
2216	92-93	-0.7744469021123699
2216	94-95	-0.8377157646147673
2216	96-97	-0.9229079376378628
2216	98-99	-1.0008694961106492
2216	100	-1.0573478552094464
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	390.0
3	40.0
4	52.0
5	152.0
6	475.0
7	1102.0
8	2126.0
9	3444.0
10	4996.0
11	7241.0
12	10521.0
13	15013.0
14	18327.0
15	24057.0
16	31397.0
17	45767.0
18	46109.0
19	22930.0
20	15776.0
21	14770.0
22	14842.0
23	15912.0
24	18462.0
25	22077.0
26	28292.0
27	36388.0
28	48064.0
29	62748.0
30	81836.0
31	106754.0
32	139191.0
33	183485.0
34	251629.0
35	371498.0
36	616209.0
37	1169719.0
38	1739193.0
39	756250.0
40	2414.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.94363519611944	21.385166275301874	15.56251252651569	22.108686002063003
2	15.409278931902874	21.242491965796283	40.22992595850546	23.118303143795384
3	17.648787921306628	26.344717258090196	31.894068585521435	24.112426235081745
4	12.49613805069036	18.30233430382377	38.96116599164065	30.24036165384522
5	12.176548749136861	38.04581792446752	35.84692793626388	13.930705390131736
6	28.705329715576372	39.2498466601941	17.89054345785858	14.15428016637095
7	25.038181018502282	34.303790039476205	22.904125168981363	17.753903773040154
8	24.4874231994884	36.0227453636371	20.753238640809705	18.73659279606479
9	24.741603548810993	16.771501444942434	22.523326848406516	35.96356815784006
10-11	23.224211622679793	27.508403534240145	29.081920208417095	20.185464634662967
12-13	24.25501480020399	25.330913403291394	29.447059926660064	20.96701186984455
14-15	21.494749772667788	27.181107546350137	27.719444729265547	23.604697951716528
16-17	19.816049789127447	30.46105772214472	28.59840078196885	21.124491706758985
18-19	19.69200364001371	28.336049627229137	31.97548151256196	19.996465220195194
20-21	21.020193778787544	27.33034068801386	31.885821890270723	19.763643642927878
22-23	20.68619418892993	27.237645472378798	32.065394100965186	20.010766237726084
24-25	20.498392147392224	27.273558228076944	31.91669218813663	20.3113574363942
26-27	20.124407047433508	27.514238619223264	31.968592402112233	20.392761931230993
28-29	20.127493233999726	27.42882039540964	32.174667029138995	20.26901934145163
30-31	20.499673842359613	27.76536651079457	31.671593322234305	20.06336632461151
32-33	20.165083998240704	27.590971673192065	32.054921303929	20.189023024638225
34-35	20.51238960558873	27.37276310499375	31.79788243754098	20.316964851876538
36-37	20.697476477524468	27.47371344808326	31.445020007933017	20.383790066459255
38-39	20.463120239177773	27.588779300221532	31.569319123158746	20.378781337441954
40-41	20.42303354263187	27.413271411726292	31.56912518247289	20.594569863168942
42-43	20.47979913816132	27.470897092036488	31.361136445198774	20.688167324603416
44-45	20.726710927866208	27.684341465125755	31.00764160031084	20.581306006697194
46-47	20.885270086858444	27.930528085309618	30.485991748582713	20.698210079249225
48-49	20.912641020175226	27.837765412044696	30.13903186158774	21.11056170619234
50-51	20.90800330812217	28.00800317320691	29.82956998459268	21.254423534078246
52-53	20.888954959889695	28.125758898335956	29.80681989892149	21.178466242852863
54-55	21.015623524364347	28.464117937523444	29.620881374408736	20.899377163703477
56-57	20.972197675140244	28.115142753836313	29.587565737460302	21.325093833563137
58-59	20.860344492624183	27.83502494583152	30.003829906935454	21.30080065460884
60-61	20.956505343993438	28.065359022997654	29.677604808919515	21.30053082408939
62-63	20.855377924625543	27.958657916962355	29.886251258084794	21.299712900327307
64-65	20.775061184070285	27.884302744446217	29.95926571020742	21.381370361276083
66-67	20.67703681567607	27.9471395266633	29.878662274725244	21.497161382935378
68-69	21.052708356381356	28.15057487392169	29.54629010018807	21.250426669508883
70-71	20.98758644695267	27.914810457551614	29.44535662150603	21.652246473989685
72-73	20.8165644908433	27.94061300097409	29.753022439105997	21.489800069076612
74-75	20.913501104955976	28.072231269039914	29.45808081693888	21.556186809065224
76-77	20.983673904420634	28.079845549010667	29.286418013345816	21.650062533222883
78-79	20.95389136083626	27.978794019476368	29.587026076421402	21.480288543265974
80-81	20.865742198030386	28.014893988935754	29.489722016031156	21.6296417970027
82-83	20.72723482811648	27.998512212815292	29.73032044342197	21.54393251564626
84-85	20.719402330381296	27.954903766769277	29.440080625597158	21.885613277252265
86-87	20.86993872883519	28.01057638641368	29.313862960462334	21.805621924288797
88-89	20.682423214508113	27.88564257790909	29.42510898043289	22.006825227149903
90-91	20.569477011664837	27.9258724342352	29.37202847536839	22.132622078731572
92-93	20.673783121330512	27.948544245028113	29.291391818522477	22.0862808151189
94-95	20.4607755268517	27.834595510835534	29.49419217899386	22.2104367833189
96-97	20.469293062743805	27.9698651903722	29.293002031664418	22.267839715219573
98-99	20.447821856148572	27.859860462527763	29.26677898471901	22.425538696604654
100	20.528065517603675	27.98397812482134	29.04306380670809	22.44489255086689
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1231.0
1	917.5
2	999.0
3	1459.5
4	1019.5
5	706.0
6	1782.0
7	4933.5
8	8499.0
9	10039.0
10	8058.0
11	5254.0
12	4926.5
13	5560.0
14	6253.0
15	6862.5
16	7512.0
17	8255.0
18	9076.0
19	10044.5
20	11551.0
21	13840.5
22	18085.5
23	25125.5
24	32637.5
25	40774.5
26	52959.5
27	69438.0
28	91807.5
29	111354.5
30	122361.0
31	136404.5
32	153513.0
33	168265.5
34	181977.5
35	194080.0
36	201962.0
37	206132.5
38	210260.5
39	213093.5
40	215297.0
41	212805.5
42	203888.5
43	197721.0
44	194227.5
45	190855.0
46	186600.5
47	180533.0
48	175711.5
49	173740.0
50	170473.0
51	162574.0
52	151415.5
53	142577.5
54	138313.0
55	133011.0
56	119357.0
57	103250.5
58	91979.5
59	82288.5
60	70256.5
61	57794.0
62	48380.0
63	39572.5
64	31163.0
65	24451.5
66	19148.0
67	14671.5
68	11057.5
69	8223.0
70	6021.0
71	4410.0
72	3218.0
73	2239.5
74	1506.5
75	981.0
76	587.0
77	369.5
78	237.5
79	143.5
80	84.5
81	37.0
82	19.5
83	16.0
84	12.0
85	5.5
86	3.5
87	4.0
88	2.5
89	1.0
90	1.0
91	0.5
92	0.5
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	3.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12160924223495223
2	0.0
3	0.0
4	0.0
5	0.01183881404090091
6	1.1805085225969567E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.264830559925311E-4
82-83	6.155508724969847E-4
84-85	2.951271306492392E-4
86-87	3.963135754432641E-4
88-89	4.8063561277161815E-4
90-91	5.39661038901466E-4
92-93	1.686440746567081E-4
94-95	2.4453390825222675E-4
96-97	4.5533900157311195E-4
98-99	9.78135633008907E-4
100	8.432203732835405E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5929648.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.05127932979293
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.15478871551909	39.72213428560232
2	16.432697016580743	18.092819872032827
3	5.280777553558222	8.721406805383026
4	2.253394330117682	4.962089628299206
5	1.1535988685111944	3.1753546772471415
6	0.6906475109117634	2.281261742497782
7	0.42563820138060765	1.640234926234414
8	0.3035021411555147	1.3366544919953987
9	0.20949693748920337	1.0379766982008871
>10	1.0015617565162327	9.544333027718487
>50	0.05707964662270226	2.146112755529857
>100	0.03121228859189767	3.3977652938719674
>500	0.0038898521026071637	1.4650586877997145
>1k	0.001592668018071061	1.3535186228076046
>5k	3.062823109398514E-5	0.12113109825508143
>10k+	9.188469328195542E-5	1.002147386524327
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23464	0.3957064567744999	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23188	0.3910518803139748	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12783	0.21557772063367	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7184	0.12115390323337913	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.6864407465670813E-5	0.0	0.0	0.0
6	0.0	1.6864407465670813E-5	0.0	0.0	0.0
7	0.0	1.6864407465670813E-5	0.0	0.0	0.0
8	0.0	1.6864407465670813E-5	0.0	0.0	0.0
9	0.0	1.6864407465670813E-5	0.0	1.6864407465670813E-5	0.0
10-11	0.0	1.6864407465670813E-5	0.0	4.2161018664177034E-5	0.0
12-13	0.0	7.588983359551865E-5	0.0	5.9025426129847844E-5	0.0
14-15	0.0	8.432203732835405E-5	0.0	6.745762986268325E-5	0.0
16-17	0.0	8.432203732835405E-5	0.0	8.432203732835405E-5	0.0
18-19	0.0	1.0118644479402487E-4	0.0	9.275424106118947E-5	0.0
20-21	0.0	1.1805085225969569E-4	0.0	1.517796671910373E-4	0.0
22-23	0.0	1.349152597253665E-4	0.0	2.951271306492392E-4	0.0
24-25	0.0	1.349152597253665E-4	0.0	7.083051135581742E-4	0.0
26-27	0.0	1.349152597253665E-4	0.0	0.0014503390420476897	0.0
28-29	0.0	1.433474634582019E-4	0.0	0.004114915421623678	0.0
30-31	0.0	1.517796671910373E-4	0.0	0.011206398760938254	0.0
32-33	0.0	1.517796671910373E-4	0.0	0.02730347568692104	0.0
34-35	0.0	1.686440746567081E-4	0.0	0.05252419705183174	0.0
36-37	0.0	2.0237288958804974E-4	0.0	0.09467678351227594	0.0
38-39	0.0	2.1080509332088514E-4	0.0	0.14951140438690458	0.0
40-41	0.0	2.1923729705372054E-4	0.0	0.20568674565505407	0.0
42-43	0.0	2.3610170451939137E-4	0.0	0.26331242596525123	0.0
44-45	0.0	2.3610170451939137E-4	0.0	0.33853611546587586	0.0
46-47	0.0	2.3610170451939137E-4	0.0	0.43191433960329517	0.0
48-49	0.0	2.3610170451939137E-4	0.0	0.5297869283303158	0.0
50-51	0.0	3.625847605119225E-4	0.0	0.6286292204866124	0.0
52-53	0.0	3.878813717104287E-4	0.0	0.7276570211250314	0.0
54-55	0.0	4.047457791760995E-4	0.0	0.8420398647609436	0.0
56-57	0.0	4.216101866417703E-4	0.0	0.9803617347943757	0.0
58-59	0.0	4.216101866417703E-4	0.0	1.1363069106294337	0.0
60-61	0.0	4.384745941074411E-4	0.0	1.2977245866871017	0.0
62-63	0.0	4.6377120530594735E-4	0.0	1.4614189577526355	0.0
64-65	0.0	4.8063561277161815E-4	0.0	1.6407466345388462	0.0
66-67	0.0	4.890678165044536E-4	0.0	1.8512313041178836	0.0
68-69	0.0	4.890678165044536E-4	0.0	2.0874173306746036	0.0
70-71	0.0	4.890678165044536E-4	0.0	2.341218230829216	0.0
72-73	0.0	5.059322239701244E-4	0.0	2.607819216250273	0.0
74-75	0.0	5.059322239701244E-4	0.0	2.880845540915751	0.0
76-77	0.0	5.059322239701244E-4	0.0	3.172186612088947	0.0
78-79	0.0	5.059322239701244E-4	0.0	3.506784888411589	0.0
80-81	0.0	5.059322239701244E-4	0.0	3.878400539121378	0.0
82-83	0.0	5.059322239701244E-4	0.0	4.253287884879507	0.0
84-85	0.0	5.059322239701244E-4	0.0	4.634254849529011	0.0
86-87	0.0	5.143644277029599E-4	0.0	5.03810681510943	0.0
88	0.0	5.227966314357952E-4	0.0	5.364348777532832	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14410	0.0	59.905956	1
GTATCAA	25205	0.0	40.224777	1
GTGGTAT	5190	0.0	38.362568	1
TGGTATC	5635	0.0	34.201237	2
ATCAACG	31975	0.0	31.400938	3
TCAACGC	32370	0.0	31.090368	4
TATCAAC	32735	0.0	30.714989	2
CAACGCA	33895	0.0	29.636082	5
AACGCAG	35975	0.0	28.062054	6
CGCAGAG	39755	0.0	25.062826	8
ACGCAGA	40460	0.0	24.881672	7
GTACATG	22205	0.0	23.423197	1
GCAGAGT	42190	0.0	23.393528	9
GAGTACT	28785	0.0	22.948301	12-13
TACATGG	22690	0.0	22.249573	2
CAGAGTA	41575	0.0	21.76128	10-11
CATGGGT	6820	0.0	21.50414	4
ACATGGG	23100	0.0	21.162807	3
GTACTTT	31400	0.0	20.894968	14-15
AGAGTAC	40020	0.0	20.48707	10-11
>>END_MODULE