FastQCFastQC Report
Wed 25 May 2016
SRR2049450_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049450_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6391226
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64650.044.734511
GTATCAA107350.035.4390871
TCAACGC115900.031.6693024
ATCAACG117000.031.2914643
CAACGCA125000.029.3658475
TATCAAC131700.028.1556472
AACGCAG132950.027.5749366
ACGCAGA149350.024.106567
CGCAGAG151250.023.4289938
GTACATG182500.022.9331231
TACATGG180950.021.842972
ACATGGG185500.020.6484743
GCAGAGT173300.019.4445679
GTGGTAT40100.018.4115921
AGAGTAC154200.017.25095410-11
CAGAGTA168950.017.1218610-11
CATGGGG130300.016.9160634
TGGTATC40450.016.6146742
GAGTACA142450.016.3410071
GTCTAGG19750.014.5244211
Produced by FastQC (version 0.11.5)