##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049450_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6391226
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.578002248707836	31.0	31.0	34.0	27.0	34.0
2	30.63852271848938	31.0	31.0	34.0	27.0	34.0
3	30.849956800150707	31.0	31.0	34.0	27.0	34.0
4	34.4355220735427	37.0	35.0	37.0	31.0	37.0
5	34.47542333818269	37.0	35.0	37.0	31.0	37.0
6	34.51515327419184	37.0	35.0	37.0	31.0	37.0
7	34.3308660967395	37.0	35.0	37.0	30.0	37.0
8	34.35256162119756	37.0	35.0	37.0	30.0	37.0
9	35.84787957740815	39.0	37.0	39.0	30.0	39.0
10-11	35.82514074764372	39.0	37.0	39.0	30.0	39.0
12-13	35.76761586900541	39.0	37.0	39.0	30.0	39.0
14-15	36.93996144088787	40.0	37.0	41.0	31.0	41.0
16-17	36.90895643496256	40.0	37.0	41.0	30.5	41.0
18-19	36.90135890985548	40.0	37.0	41.0	30.0	41.0
20-21	36.9764793171138	40.0	37.0	41.0	30.0	41.0
22-23	36.91652532706557	40.0	37.0	41.0	30.0	41.0
24-25	36.91797160669956	40.0	37.0	41.0	30.0	41.0
26-27	36.92677742893147	40.0	37.0	41.0	30.0	41.0
28-29	36.906981227075995	40.0	37.0	41.0	30.0	41.0
30-31	36.8489655192916	40.0	37.0	41.0	30.0	41.0
32-33	36.82178583576922	40.0	37.0	41.0	30.0	41.0
34-35	36.753693501059104	40.0	37.0	41.0	30.0	41.0
36-37	36.62980435991467	40.0	37.0	41.0	30.0	41.0
38-39	36.518498485267145	40.0	36.0	41.0	30.0	41.0
40-41	36.40507243837098	40.0	36.0	41.0	30.0	41.0
42-43	36.28543302959401	40.0	36.0	41.0	29.5	41.0
44-45	36.09673988996791	40.0	36.0	41.0	29.0	41.0
46-47	35.968433912366734	39.0	35.0	41.0	28.5	41.0
48-49	35.862668836933636	39.0	35.0	41.0	28.0	41.0
50-51	35.74555679927451	39.0	35.0	41.0	28.0	41.0
52-53	35.63191068505479	39.0	35.0	41.0	28.0	41.0
54-55	35.48516161062056	39.0	35.0	40.0	28.0	41.0
56-57	35.31882294570713	39.0	35.0	40.0	27.5	41.0
58-59	35.132016220362104	38.5	34.5	40.0	27.0	41.0
60-61	34.93605874678818	38.0	34.0	40.0	26.5	41.0
62-63	34.684578905518286	38.0	34.0	40.0	26.0	41.0
64-65	34.446193500276785	37.5	34.0	40.0	26.0	41.0
66-67	34.228651748506465	37.0	34.0	40.0	26.0	41.0
68-69	33.94966654285109	37.0	33.5	40.0	26.0	41.0
70-71	33.64021715082521	36.0	33.0	39.0	25.5	41.0
72-73	33.31093416192762	36.0	33.0	39.0	25.0	40.5
74-75	33.015077545372364	35.0	33.0	39.0	24.5	40.0
76-77	32.693252828174124	35.0	33.0	37.5	24.0	40.0
78-79	32.34968955877949	35.0	32.0	37.0	23.5	39.0
80-81	32.01788506618292	35.0	32.0	37.0	22.5	39.0
82-83	31.722956675292032	35.0	32.0	36.0	22.0	38.5
84-85	31.43359896833565	35.0	32.0	36.0	21.5	37.0
86-87	31.157893180432048	35.0	32.0	35.5	20.0	37.0
88-89	30.92579326720726	35.0	32.0	35.0	20.0	36.5
90-91	30.714028654283233	35.0	32.0	35.0	18.5	36.0
92-93	30.499894855853945	34.5	31.0	35.0	17.5	36.0
94-95	30.283902884986386	34.0	31.0	35.0	9.5	36.0
96-97	30.077321862816305	34.0	31.0	35.0	2.0	35.5
98-99	29.88534594145161	34.0	31.0	35.0	2.0	35.0
100	29.73725135052336	34.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5679440237745368
1101	2	0.5001814500664956
1101	3	0.5690788045701396
1101	4	0.6572615131937596
1101	5	0.6510562481486346
1101	6	0.6703793081301441
1101	7	0.7394775043892494
1101	8	0.7170928083042938
1101	9	0.8617617763488354
1101	10-11	0.9146253393222921
1101	12-13	0.8857807839621188
1101	14-15	1.0499487550123092
1101	16-17	0.9895555830018594
1101	18-19	0.9964026301546554
1101	20-21	1.0211882869916522
1101	22-23	0.9566050787964571
1101	24-25	1.0401234956700733
1101	26-27	0.9869473887044791
1101	28-29	0.9463452703449846
1101	30-31	0.9574264104567689
1101	32-33	0.9445982315796755
1101	34-35	0.9223133829914971
1101	36-37	0.9380543675653996
1101	38-39	0.9607838178069485
1101	40-41	0.9957051391893046
1101	42-43	0.9232287815496747
1101	44-45	0.8858338056742525
1101	46-47	0.8456813772000018
1101	48-49	0.8313195656886876
1101	50-51	0.8208315556892245
1101	52-53	0.8171912628182127
1101	54-55	0.8230258621574507
1101	56-57	0.780451193386746
1101	58-59	0.8284699286971602
1101	60-61	0.8569200746799339
1101	62-63	0.8264559672477532
1101	64-65	0.8427537405355849
1101	66-67	0.84592302143718
1101	68-69	0.790630674603328
1101	70-71	0.8217285240394645
1101	72-73	0.8127558377213688
1101	74-75	0.7659209654021666
1101	76-77	0.746419868815515
1101	78-79	0.7347741519403357
1101	80-81	0.7755488172660705
1101	82-83	0.7689383228245035
1101	84-85	0.7708618608364581
1101	86-87	0.8315562795587539
1101	88-89	0.741399104344282
1101	90-91	0.7875968822145509
1101	92-93	0.7431087710011788
1101	94-95	0.7759892169875187
1101	96-97	0.7970671932500615
1101	98-99	0.8720523079462161
1101	100	0.9257521293099309
1102	1	0.4314689281180186
1102	2	0.4788392739690366
1102	3	0.5463149433067791
1102	4	0.5653291218807652
1102	5	0.5056687816019476
1102	6	0.5633617537661095
1102	7	0.5198147382745049
1102	8	0.5634437528746759
1102	9	0.6853889165341513
1102	10-11	0.7497240247243298
1102	12-13	0.7168168260476193
1102	14-15	0.8223269791332868
1102	16-17	0.7171470131358433
1102	18-19	0.752767343998606
1102	20-21	0.7793735847169359
1102	22-23	0.7611956638515309
1102	24-25	0.7619002579698346
1102	26-27	0.6917011653332708
1102	28-29	0.7458744251215919
1102	30-31	0.7776900346942526
1102	32-33	0.7066667331466121
1102	34-35	0.7235796677578463
1102	36-37	0.7743769155450835
1102	38-39	0.8757677450773116
1102	40-41	0.8176194457743122
1102	42-43	0.7685501728034936
1102	44-45	0.7183544264306718
1102	46-47	0.7406632771321284
1102	48-49	0.655620905938882
1102	50-51	0.7273894975801767
1102	52-53	0.701316412678942
1102	54-55	0.7123780758801743
1102	56-57	0.7162369371363368
1102	58-59	0.7626020210903448
1102	60-61	0.7348321651966998
1102	62-63	0.6872994483235928
1102	64-65	0.7231857773489239
1102	66-67	0.7609162887146894
1102	68-69	0.7078782361068434
1102	70-71	0.6663378479941642
1102	72-73	0.7019184678555064
1102	74-75	0.6941864817890249
1102	76-77	0.6515824754944717
1102	78-79	0.658873864322544
1102	80-81	0.7306532214740713
1102	82-83	0.6921422852241861
1102	84-85	0.6413013889540586
1102	86-87	0.6199941820450228
1102	88-89	0.5964753598738142
1102	90-91	0.6228345296035762
1102	92-93	0.6414401830558702
1102	94-95	0.5875669686006333
1102	96-97	0.6271847600641429
1102	98-99	0.7060893994773956
1102	100	0.6395370248136842
1103	1	0.5145537886526661
1103	2	0.47743932378414655
1103	3	0.5281479970476894
1103	4	0.5307053916046129
1103	5	0.5674022022781173
1103	6	0.5985995763302654
1103	7	0.598275539610043
1103	8	0.5637234085343152
1103	9	0.728531680315605
1103	10-11	0.7171051771162382
1103	12-13	0.7330208824526636
1103	14-15	0.8075343271156825
1103	16-17	0.7936866506409217
1103	18-19	0.776131261159442
1103	20-21	0.7845541628287975
1103	22-23	0.7751816483185436
1103	24-25	0.7997453819264777
1103	26-27	0.8094917615942379
1103	28-29	0.7885246937653321
1103	30-31	0.7609997201189245
1103	32-33	0.7850231186356922
1103	34-35	0.8086693279590946
1103	36-37	0.7655383501035615
1103	38-39	0.8030883457641593
1103	40-41	0.8264499719308134
1103	42-43	0.7883754053872352
1103	44-45	0.7589899826610917
1103	46-47	0.7185085253538261
1103	48-49	0.6933038865282342
1103	50-51	0.6468126153244782
1103	52-53	0.6326063095576799
1103	54-55	0.5887801633528653
1103	56-57	0.6411911066901723
1103	58-59	0.7017783617164142
1103	60-61	0.6510001135917918
1103	62-63	0.6588700167999377
1103	64-65	0.6633316225480144
1103	66-67	0.6742959213734352
1103	68-69	0.6243981877980858
1103	70-71	0.6590380230489998
1103	72-73	0.6624558112948762
1103	74-75	0.624090669163273
1103	76-77	0.5698356282637249
1103	78-79	0.5715742663271115
1103	80-81	0.5876219974364858
1103	82-83	0.5609909960536825
1103	84-85	0.5535551422745009
1103	86-87	0.5525405373569008
1103	88-89	0.5289637445891273
1103	90-91	0.5321461654678856
1103	92-93	0.5180255496267563
1103	94-95	0.5917620524995826
1103	96-97	0.6149172201999029
1103	98-99	0.5590519258087241
1103	100	0.5469875780528533
1104	1	0.4251768142886867
1104	2	0.4228340909751118
1104	3	0.4700859807216773
1104	4	0.5028665460384403
1104	5	0.4926624762346634
1104	6	0.5074173902917494
1104	7	0.499151283271857
1104	8	0.5246645836948645
1104	9	0.6271266974733507
1104	10-11	0.6656124470179066
1104	12-13	0.6659256284278783
1104	14-15	0.7750663095763528
1104	16-17	0.7290907657201728
1104	18-19	0.7281617712440251
1104	20-21	0.7353869764874972
1104	22-23	0.7382256785167485
1104	24-25	0.7404429200634368
1104	26-27	0.7519891297288126
1104	28-29	0.7315212392021024
1104	30-31	0.7310848940881414
1104	32-33	0.6881654724657693
1104	34-35	0.714490105765023
1104	36-37	0.7268697503761103
1104	38-39	0.7532822332138096
1104	40-41	0.8025339631032296
1104	42-43	0.7682196728435713
1104	44-45	0.7342526848528053
1104	46-47	0.6704042568342317
1104	48-49	0.7099644650437398
1104	50-51	0.6633914205270557
1104	52-53	0.6932227064328984
1104	54-55	0.6399090977263882
1104	56-57	0.6127469548987463
1104	58-59	0.6469047156575698
1104	60-61	0.6995604214692435
1104	62-63	0.6767635177752993
1104	64-65	0.6638600355987734
1104	66-67	0.7085725430161105
1104	68-69	0.6788511388735898
1104	70-71	0.6561427146754752
1104	72-73	0.6670755272953883
1104	74-75	0.669637959277054
1104	76-77	0.5983252115518596
1104	78-79	0.595674153812304
1104	80-81	0.6251901766616754
1104	82-83	0.5796412453335265
1104	84-85	0.5543246206398855
1104	86-87	0.5760246313909221
1104	88-89	0.5986134749626046
1104	90-91	0.6368553570598756
1104	92-93	0.6355058775688995
1104	94-95	0.5926472453717295
1104	96-97	0.682695761230331
1104	98-99	0.6847515226766028
1104	100	0.641768285763316
1105	1	0.4037957386576174
1105	2	0.37166208866323913
1105	3	0.4035187262562303
1105	4	0.39596200804766823
1105	5	0.40806043437286377
1105	6	0.4328280261861295
1105	7	0.45209135193184835
1105	8	0.47456746830720675
1105	9	0.5145462476230449
1105	10-11	0.5891982454432139
1105	12-13	0.5485685810673644
1105	14-15	0.6773430628443506
1105	16-17	0.6297072883095325
1105	18-19	0.6544449125997502
1105	20-21	0.6348441701566756
1105	22-23	0.6560656131977751
1105	24-25	0.6155314619534451
1105	26-27	0.6914943993610834
1105	28-29	0.6892247846202579
1105	30-31	0.6731100628002835
1105	32-33	0.6435958107651274
1105	34-35	0.6837160446789454
1105	36-37	0.6881859598459386
1105	38-39	0.7308929055298705
1105	40-41	0.7374870246356906
1105	42-43	0.7111693553868363
1105	44-45	0.7155724873901264
1105	46-47	0.6746961503044133
1105	48-49	0.5838695205540674
1105	50-51	0.5673140071079246
1105	52-53	0.5824149929364424
1105	54-55	0.5516736550234924
1105	56-57	0.5583042846200286
1105	58-59	0.5578339256813649
1105	60-61	0.5891709789991921
1105	62-63	0.6753098802936677
1105	64-65	0.5691234321048526
1105	66-67	0.6059534117702938
1105	68-69	0.6087355365917944
1105	70-71	0.6435921561351492
1105	72-73	0.5938579009916509
1105	74-75	0.5776932335957028
1105	76-77	0.5379917109493988
1105	78-79	0.5345473632660998
1105	80-81	0.6008871177315456
1105	82-83	0.5837625581157724
1105	84-85	0.5815634601172768
1105	86-87	0.5270724039127614
1105	88-89	0.5583874423212265
1105	90-91	0.6094466624912336
1105	92-93	0.5550471564368742
1105	94-95	0.5439458342989454
1105	96-97	0.6122895056485582
1105	98-99	0.5778499798180086
1105	100	0.5685533436217405
1106	1	0.41155114378133106
1106	2	0.35885175094087174
1106	3	0.43191432165797394
1106	4	0.4377350865114238
1106	5	0.42990225423313433
1106	6	0.4211870414819572
1106	7	0.45716780223987286
1106	8	0.3859757927789005
1106	9	0.5424990008812003
1106	10-11	0.5183265956977294
1106	12-13	0.5384884948428237
1106	14-15	0.6261125188401522
1106	16-17	0.6104491785826553
1106	18-19	0.6363157621554194
1106	20-21	0.6249806539132052
1106	22-23	0.6319621283855312
1106	24-25	0.6286939600442238
1106	26-27	0.6173636811979293
1106	28-29	0.5674899289089907
1106	30-31	0.5768351721419407
1106	32-33	0.5604016220836385
1106	34-35	0.5329863157540586
1106	36-37	0.5140748861986992
1106	38-39	0.5283756217180127
1106	40-41	0.6750702016294099
1106	42-43	0.620851075804552
1106	44-45	0.5692408245611063
1106	46-47	0.5320846976957299
1106	48-49	0.5021000155670521
1106	50-51	0.537989211694267
1106	52-53	0.5524128699838684
1106	54-55	0.5708982393906794
1106	56-57	0.5594245023331368
1106	58-59	0.5875066547705288
1106	60-61	0.5754995486574899
1106	62-63	0.6091709738736029
1106	64-65	0.5981951542804822
1106	66-67	0.6793713558107939
1106	68-69	0.6544994296833622
1106	70-71	0.6717181557720338
1106	72-73	0.6530160398250615
1106	74-75	0.6481028769877
1106	76-77	0.5305063818965507
1106	78-79	0.5300492494278117
1106	80-81	0.555145366747162
1106	82-83	0.5602406278235534
1106	84-85	0.593192293079337
1106	86-87	0.593608763558219
1106	88-89	0.5611714880924694
1106	90-91	0.640522893779714
1106	92-93	0.527993343911838
1106	94-95	0.5900349906222218
1106	96-97	0.6371494243999898
1106	98-99	0.709143597607774
1106	100	0.6997868751293339
1107	1	0.3355896061812942
1107	2	0.3398078693308335
1107	3	0.3705020978278135
1107	4	0.3970501509486297
1107	5	0.3483243951229298
1107	6	0.41889566273393797
1107	7	0.41157034341881626
1107	8	0.3771597963650777
1107	9	0.4116981348327329
1107	10-11	0.4371190445878881
1107	12-13	0.4817516979698908
1107	14-15	0.5046214383513288
1107	16-17	0.47641737796574546
1107	18-19	0.5019542374488921
1107	20-21	0.537226940399421
1107	22-23	0.5319682011262756
1107	24-25	0.558450081317936
1107	26-27	0.6048440005276206
1107	28-29	0.5670609354589402
1107	30-31	0.5365387086273614
1107	32-33	0.5299046545680355
1107	34-35	0.5594159898844708
1107	36-37	0.5455563534420378
1107	38-39	0.5222100969120476
1107	40-41	0.6187210433525365
1107	42-43	0.5244099675440594
1107	44-45	0.5302027383606145
1107	46-47	0.507468256022598
1107	48-49	0.5180129195670915
1107	50-51	0.6060834975108946
1107	52-53	0.5359041779217932
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2210	16-17	0.05071155317089904
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2210	30-31	0.1407395461677936
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2211	2	-0.08437451970326393
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2211	4	-0.120468355807688
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2211	10-11	-0.17213358487315844
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2211	30-31	-0.0996561292217848
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2211	64-65	0.05729481973030204
2211	66-67	-5.887899456524792E-4
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2211	72-73	0.034539851243536646
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2211	86-87	0.09900334377897835
2211	88-89	0.01690111584322551
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2212	9	-0.13384405447242642
2212	10-11	-0.15692630448984346
2212	12-13	-0.16872149962333793
2212	14-15	-0.18539124998485335
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2212	58-59	-0.46080278129454655
2212	60-61	-0.07858754681765134
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2212	64-65	0.0116008691821321
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2212	72-73	0.08499477766788743
2212	74-75	0.06187001367995748
2212	76-77	0.08806536811429311
2212	78-79	0.07773465321680106
2212	80-81	-0.12211558158690394
2212	82-83	-0.08482251663180307
2212	84-85	0.0041965015660387905
2212	86-87	0.08510450110555823
2212	88-89	0.08614232860756488
2212	90-91	0.12299705337969868
2212	92-93	0.1409000702998746
2212	94-95	0.1825039880748882
2212	96-97	0.17971881282348434
2212	98-99	0.2100380886630795
2212	100	0.203416146320194
2213	1	-0.39454611758813485
2213	2	-0.4578324155575899
2213	3	-0.6132142051279459
2213	4	-0.4854602666202794
2213	5	-0.5669081082971914
2213	6	-0.5672573714036204
2213	7	-0.6405577456078717
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2213	9	-0.734425482029998
2213	10-11	-0.774604699546245
2213	12-13	-0.791995225930421
2213	14-15	-0.7899686102485575
2213	16-17	-0.7979553649303455
2213	18-19	-0.7206286466757135
2213	20-21	-0.766595560429387
2213	22-23	-0.8801725859113603
2213	24-25	-1.0596278992290706
2213	26-27	-0.9317243879931425
2213	28-29	-0.7364809440071696
2213	30-31	-0.7435580382301339
2213	32-33	-0.6683776128811374
2213	34-35	-0.679560373293107
2213	36-37	-0.58284886910522
2213	38-39	-0.641091785091831
2213	40-41	-0.6768033104191673
2213	42-43	-0.7864615088866316
2213	44-45	-0.670281027874438
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2213	48-49	-0.6862714831790342
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2213	56-57	-0.6507411357116055
2213	58-59	-0.7223558647099537
2213	60-61	-0.7497422218740226
2213	62-63	-0.8002344679713929
2213	64-65	-0.787828890510319
2213	66-67	-0.6320396303140399
2213	68-69	-0.6289934622290403
2213	70-71	-0.6579104072846675
2213	72-73	-0.6476759979815938
2213	74-75	-0.6672660078511399
2213	76-77	-0.7003912422245904
2213	78-79	-0.6519278524203358
2213	80-81	-0.8109824541281974
2213	82-83	-0.7851836976220383
2213	84-85	-0.7646829928407435
2213	86-87	-0.7113637747458128
2213	88-89	-0.63120667220511
2213	90-91	-0.6377053649530531
2213	92-93	-0.6453071506638715
2213	94-95	-0.6686108796256747
2213	96-97	-0.9065935803244152
2213	98-99	-0.9617637922304034
2213	100	-0.9412877394634407
2214	1	-0.5147262152787526
2214	2	-0.48544704904751157
2214	3	-0.5115115564450647
2214	4	-0.5623687059380131
2214	5	-0.4782270952694958
2214	6	-0.5289169100111266
2214	7	-0.5729925670229647
2214	8	-0.5390790382246138
2214	9	-0.6286089643653696
2214	10-11	-0.7241469648015055
2214	12-13	-0.7498767338942116
2214	14-15	-0.7758925303157227
2214	16-17	-0.740147547538669
2214	18-19	-0.645577401770268
2214	20-21	-0.675509963744382
2214	22-23	-0.6925465128511306
2214	24-25	-0.6943977481558861
2214	26-27	-0.7051407324515253
2214	28-29	-0.7948219335130275
2214	30-31	-0.7565978081540123
2214	32-33	-0.8358557919618832
2214	34-35	-0.8637697048338353
2214	36-37	-0.7708173797985438
2214	38-39	-0.9220673186064232
2214	40-41	-1.0085863498047587
2214	42-43	-1.1687309591820778
2214	44-45	-1.1353009105531413
2214	46-47	-1.0208586788694305
2214	48-49	-1.0293685959267478
2214	50-51	-0.8922669771316833
2214	52-53	-0.8455019953252645
2214	54-55	-0.7504970283248582
2214	56-57	-0.8964011761541215
2214	58-59	-0.945975651642442
2214	60-61	-1.0020507741173645
2214	62-63	-1.030189837044972
2214	64-65	-1.0186128297257113
2214	66-67	-1.0518997347650512
2214	68-69	-1.07678789463764
2214	70-71	-0.8942365957642338
2214	72-73	-0.823506092114954
2214	74-75	-0.7006307280825297
2214	76-77	-0.7145192974414485
2214	78-79	-0.744143868032662
2214	80-81	-0.6852703477515334
2214	82-83	-0.6144214971211781
2214	84-85	-0.6797773834704941
2214	86-87	-0.6951990582422773
2214	88-89	-0.7277646038218819
2214	90-91	-0.7053991724987974
2214	92-93	-0.6325145551085853
2214	94-95	-0.5819953101477608
2214	96-97	-0.48154261042495605
2214	98-99	-0.5622144209560638
2214	100	-0.5520843016237009
2215	1	-0.6026211991651422
2215	2	-0.6608722504075075
2215	3	-0.6947354933831988
2215	4	-0.6803661434449495
2215	5	-0.5814970542839291
2215	6	-0.6761301782714071
2215	7	-0.6970225102407142
2215	8	-0.7030297764103466
2215	9	-0.8659284887288123
2215	10-11	-0.8618043119658694
2215	12-13	-0.8853748285205114
2215	14-15	-1.0420920626147208
2215	16-17	-1.1691688292200624
2215	18-19	-1.0840818977005071
2215	20-21	-0.9992145895041986
2215	22-23	-1.0288325538925633
2215	24-25	-1.0828670415330137
2215	26-27	-1.1221275472986676
2215	28-29	-1.015973241323458
2215	30-31	-1.2486890190194089
2215	32-33	-1.1875910614867635
2215	34-35	-1.0175197369165474
2215	36-37	-1.0882556879046774
2215	38-39	-1.0766143892321338
2215	40-41	-1.0368220086054976
2215	42-43	-1.0384679670996775
2215	44-45	-0.954167433774245
2215	46-47	-0.8665476540319688
2215	48-49	-0.8402128481537616
2215	50-51	-0.943156539622052
2215	52-53	-0.8920366699783884
2215	54-55	-0.9612223804648039
2215	56-57	-0.8507122864896033
2215	58-59	-0.9403179918905948
2215	60-61	-0.9427217689621159
2215	62-63	-0.9823712880547006
2215	64-65	-0.9657834051356105
2215	66-67	-0.8785675752692654
2215	68-69	-0.8972939681572498
2215	70-71	-0.836903785008225
2215	72-73	-0.7537909339711888
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2215	78-79	-0.7193899401403883
2215	80-81	-0.7649957274352559
2215	82-83	-0.781327880961733
2215	84-85	-0.7361050937330553
2215	86-87	-0.7241262158221566
2215	88-89	-0.6919418380021227
2215	90-91	-0.7015101399117647
2215	92-93	-0.7631915881253875
2215	94-95	-0.8862820024158893
2215	96-97	-0.8852425674898647
2215	98-99	-0.880015500521143
2215	100	-0.9791835332932131
2216	1	-1.5788371879346883
2216	2	-1.6914577825291808
2216	3	-1.7179525828591267
2216	4	-1.8319063060750267
2216	5	-1.7314110220013745
2216	6	-1.8311170841921154
2216	7	-1.8081465536824979
2216	8	-1.7906824050383676
2216	9	-2.146169499120404
2216	10-11	-2.156619522015724
2216	12-13	-2.1547861348124897
2216	14-15	-2.4550124833226477
2216	16-17	-2.505506827064913
2216	18-19	-2.6135387028672383
2216	20-21	-2.4835914679067272
2216	22-23	-2.5708887956033735
2216	24-25	-2.562664556762364
2216	26-27	-2.623292803520812
2216	28-29	-2.506984022682495
2216	30-31	-2.6644870313340476
2216	32-33	-2.613968874626565
2216	34-35	-2.5950109741207825
2216	36-37	-2.6956948249817145
2216	38-39	-2.6678547616654953
2216	40-41	-2.7395680164247835
2216	42-43	-2.6786043085737603
2216	44-45	-2.702526850620117
2216	46-47	-2.5290625039680705
2216	48-49	-2.652474302991642
2216	50-51	-2.559247046862666
2216	52-53	-2.7687919350472185
2216	54-55	-2.6647322005211223
2216	56-57	-2.65991254432614
2216	58-59	-2.6821914984461728
2216	60-61	-2.715905851230943
2216	62-63	-2.7272214290982078
2216	64-65	-2.690076067986624
2216	66-67	-2.69652257372546
2216	68-69	-2.663514283273301
2216	70-71	-2.619355586147236
2216	72-73	-2.6609880896924594
2216	74-75	-2.6709440215931153
2216	76-77	-2.654566715939943
2216	78-79	-2.710586363121976
2216	80-81	-2.6847937889973146
2216	82-83	-2.6389828511101108
2216	84-85	-2.619097731177046
2216	86-87	-2.8065454316453717
2216	88-89	-2.7961022654727863
2216	90-91	-2.8441359188197275
2216	92-93	-2.8668534326736648
2216	94-95	-2.845528762311936
2216	96-97	-3.004110465971422
2216	98-99	-3.118857021205624
2216	100	-3.2505412136193605
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	118628.0
3	22304.0
4	13995.0
5	11112.0
6	9548.0
7	9584.0
8	10229.0
9	11755.0
10	13448.0
11	14676.0
12	15961.0
13	17949.0
14	19515.0
15	20723.0
16	20927.0
17	22146.0
18	21095.0
19	19931.0
20	20328.0
21	21657.0
22	24289.0
23	30404.0
24	39003.0
25	48900.0
26	57957.0
27	64686.0
28	76216.0
29	95198.0
30	114427.0
31	133828.0
32	170396.0
33	225314.0
34	312810.0
35	456662.0
36	759793.0
37	1294437.0
38	1572499.0
39	478287.0
40	609.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.78916021180213	20.664799506836815	14.714992984449921	22.831047296911137
2	15.221441840093174	20.65731115714849	40.51377217192067	23.60747483083766
3	18.035659374493854	25.700914762248665	30.6063908947863	25.657034968471176
4	12.557561429632816	17.51352462787322	39.23695936696667	30.69195457552729
5	12.970278593201348	38.04717181896136	35.59542959434677	13.38711999349052
6	30.04412600007198	38.334838102957534	18.23605532336378	13.384980573606706
7	26.47630720654725	32.78363425493753	22.98271309413909	17.757345444376128
8	24.307711470189126	36.462005342773736	21.058718560505536	18.1715646265316
9	24.442135870282126	17.653034447567226	22.11378808697493	35.79104159517571
10-11	23.20905846220869	27.560383319188514	29.406045228935167	19.82451298966762
12-13	23.783538861337274	25.12531630802449	29.26138628688865	21.829758543749584
14-15	21.31611719436491	27.442765241588408	26.420620559955616	24.820497004091067
16-17	20.873176162427377	30.6399313540802	27.066417368538126	21.420475114954296
18-19	20.79109580842165	29.64980754193996	28.995274884313137	20.56382176532525
20-21	21.951379812305934	28.801943211376656	28.530679165520752	20.71599781079665
22-23	21.620582903085804	28.676757150420933	28.519286828287477	21.183373118205786
24-25	21.44076520156288	28.810327711030403	28.455412796717006	21.29349429068971
26-27	21.179565788616177	28.783675025166566	28.56613199399554	21.470627192221713
28-29	21.235260170239986	28.754615439023002	28.730791041964114	21.2793333487729
30-31	21.52181199547086	29.08924059229382	28.29297882581512	21.095968586420206
32-33	21.190230214390848	28.876971331409013	28.658427429059298	21.27437102514084
34-35	21.415550889941716	28.59172918889125	28.66984236145259	21.322877559714446
36-37	21.616679213668373	28.85576977694086	28.225230129641222	21.302320879749544
38-39	21.44688394041392	28.988382640854347	28.361102680034012	21.203630738697722
40-41	21.38843226097713	28.70455030909428	28.49923596870014	21.407781461228442
42-43	21.322761067330795	28.705263829919076	28.500913664852273	21.47106143789786
44-45	21.481216831420458	28.799789107634684	28.39811395621229	21.320880104732566
46-47	21.498032941914374	28.845853051578867	28.264330917392638	21.39178308911412
48-49	21.386940130921065	28.767838112332967	28.30748609961331	21.53773565713266
50-51	21.442967006167727	28.6973444683276	28.262972346821392	21.59671617868328
52-53	21.409575945483823	28.689538115020817	28.27936645240984	21.621519487085518
54-55	21.55252738517318	28.95909935729294	28.14464644852417	21.343726809009713
56-57	21.48039830008219	28.72909453523624	28.164669678476663	21.62583748620491
58-59	21.374088322469532	28.429200718561415	28.46949110349184	21.727219855477216
60-61	21.585678573753338	28.775034361809197	28.161004791876344	21.478282272561124
62-63	21.41543546642267	28.846993157920846	28.206100831743818	21.531470543912665
64-65	21.470858729962604	28.589077484664994	28.26993152036098	21.67013226501142
66-67	21.351503014040066	28.636400765770542	28.202634691333568	21.809461528855824
68-69	21.6689243679082	28.75587634134878	28.15231780358805	21.422881487154967
70-71	21.646705736373303	28.553167011766845	28.205959271423243	21.59416798043661
72-73	21.444827361987127	28.69895646688418	28.353360408558753	21.502855762569943
74-75	21.49092403759728	28.731967774251686	28.186841171029624	21.590267017121406
76-77	21.530635684747036	28.723235125304896	28.002314943764738	21.743814246183334
78-79	21.594769644289087	28.7272101632092	28.217957463848325	21.46006272865339
80-81	21.485661864413807	28.65900979141272	28.24396199867073	21.611366345502738
82-83	21.403144168107087	28.717292455141997	28.363931887624403	21.515631489126513
84-85	21.442558619059014	28.620691112067036	28.243657305383064	21.693092963490884
86-87	21.52908794205134	28.738415087584002	28.137675643734916	21.59482132662974
88-89	21.395176629164393	28.643706378871116	28.20168650303854	21.75943048892595
90-91	21.34132400709014	28.61208963671875	28.264475923836237	21.782110432354873
92-93	21.41792603113575	28.78872008345178	28.140541733987206	21.652812151425266
94-95	21.311186421475124	28.605581916269458	28.278445295926407	21.80478636632901
96-97	21.36616905348756	28.659697714038114	28.229899385629064	21.744233846845265
98-99	21.30491353336103	28.715679163898937	28.179760476886813	21.799646825853223
100	21.362055041943893	28.63668533465064	28.171203631971526	21.830055991433937
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2944.0
1	2392.0
2	1941.0
3	1954.0
4	1672.0
5	1350.0
6	1140.0
7	1095.0
8	1161.5
9	1164.5
10	1064.5
11	978.0
12	1003.0
13	1109.5
14	1209.5
15	1333.5
16	1589.5
17	1969.0
18	2649.5
19	3684.0
20	5239.0
21	7670.5
22	11625.0
23	17617.5
24	25580.5
25	36045.5
26	49824.0
27	66392.0
28	86212.5
29	107999.0
30	128310.5
31	146682.0
32	163309.0
33	178148.5
34	192375.0
35	203739.0
36	212051.0
37	220341.0
38	227833.0
39	231280.0
40	232684.0
41	232081.5
42	228142.5
43	223997.5
44	219219.0
45	214479.5
46	210557.5
47	204478.5
48	198757.5
49	195883.0
50	190423.0
51	180965.5
52	168322.0
53	157167.5
54	149733.5
55	142921.5
56	131445.5
57	117475.0
58	105811.5
59	95313.0
60	83936.5
61	70816.0
62	59064.5
63	48763.0
64	39792.0
65	32148.5
66	25511.5
67	19998.5
68	15469.5
69	11939.0
70	9035.5
71	6715.5
72	5133.0
73	3735.5
74	2631.0
75	1917.0
76	1295.0
77	856.5
78	633.5
79	452.5
80	329.0
81	222.0
82	151.0
83	122.0
84	95.0
85	70.0
86	54.5
87	48.0
88	35.5
89	22.5
90	21.0
91	20.5
92	18.0
93	18.5
94	16.0
95	11.0
96	6.5
97	6.0
98	8.0
99	10.5
100	18.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0735226699853831
2	0.012000827384292152
3	0.01628795476798974
4	0.01035795010221826
5	0.008542961866784245
6	0.006821852333183023
7	0.019479830630304736
8	0.00841779026434052
9	0.008715072820144366
10-11	0.012227700913721405
12-13	0.014027042698849955
14-15	0.00930181470659933
16-17	0.011758307404557435
18-19	0.007557235497539909
20-21	0.01210252931127768
22-23	0.012759680224107237
24-25	0.015051885193857953
26-27	0.004795637018625221
28-29	0.011234151319324337
30-31	0.009606920487555909
32-33	0.011703544828488305
34-35	0.007799755477274626
36-37	0.009215759229919268
38-39	0.018408048784380338
40-41	0.012219877688568673
42-43	0.007510296146623512
44-45	0.019346835802708275
46-47	0.01720327211085948
48-49	0.017461438540899665
50-51	0.009739915315152368
52-53	0.009528688236028579
54-55	0.010616116532258442
56-57	0.019870991887941374
58-59	0.001924513387572275
60-61	0.006415044625240915
62-63	0.014347794930112
64-65	0.023727841888238656
66-67	0.012517160244372522
68-69	0.013714113692740643
70-71	0.024025124444042503
72-73	0.01958153255729026
74-75	0.01944071450454107
76-77	0.008981062475337283
78-79	0.010725641684396702
80-81	0.01938595192847194
82-83	0.011390615822378993
84-85	0.01071781845924397
86-87	0.01592808641096403
88-89	0.014120921400682747
90-91	0.014754602638054106
92-93	0.007346008418416122
94-95	0.014543375558930321
96-97	0.012313756390401465
98-99	0.009559981136639513
100	0.006681034280433832
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6391226.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.3157938609475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.64496520700753	48.52836817526382
2	14.47430583840254	18.329043294892085
3	4.228817219229989	8.032527579851736
4	1.7026770754846217	4.31225402892581
5	0.8746691495539651	2.769018578484456
6	0.5087092917049075	1.9325599589241922
7	0.320891370769432	1.4222244318375963
8	0.23220962352090688	1.1762029324302374
9	0.16502633286879187	0.9403895946193678
>10	0.7914125549363283	8.77107399775804
>50	0.038604550815351184	1.657090001362119
>100	0.01709347804429716	1.8191961320027354
>500	4.94654052847569E-4	0.18683492924267894
>1k	1.236536086114785E-4	0.12321636440517486
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.564645030546565E-5	0.0
3	0.0	0.0	0.0	1.564645030546565E-5	0.0
4	0.0	0.0	0.0	1.564645030546565E-5	0.0
5	0.0	0.0	0.0	1.564645030546565E-5	0.0
6	0.0	0.0	0.0	1.564645030546565E-5	0.0
7	0.0	0.0	0.0	4.6939350916396946E-5	0.0
8	1.564645030546565E-5	0.0	0.0	6.25858012218626E-5	0.0
9	1.564645030546565E-5	0.0	0.0	6.25858012218626E-5	0.0
10-11	1.564645030546565E-5	7.823225152732825E-6	0.0	6.25858012218626E-5	0.0
12-13	1.564645030546565E-5	3.12929006109313E-5	0.0	7.040902637459542E-5	0.0
14-15	2.3469675458198476E-5	6.25858012218626E-5	0.0	7.823225152732824E-5	0.0
16-17	3.12929006109313E-5	1.1734837729099237E-4	0.0	8.605547668006107E-5	0.0
18-19	3.12929006109313E-5	1.4081805274919083E-4	0.0	1.1734837729099237E-4	0.0
20-21	3.12929006109313E-5	1.4081805274919083E-4	0.0	1.251716024437252E-4	0.0
22-23	4.693935091639695E-5	1.4081805274919083E-4	0.0	1.955806288183206E-4	0.0
24-25	6.25858012218626E-5	1.5646450305465648E-4	0.0	2.581664300401832E-4	0.0
26-27	6.25858012218626E-5	1.5646450305465648E-4	0.0	4.693935091639695E-4	0.0
28-29	6.25858012218626E-5	2.268735294292519E-4	0.0	0.001024842495008	0.0
30-31	8.605547668006107E-5	2.503432048874504E-4	0.0	0.0026598965519291605	0.0
32-33	1.0952515213825954E-4	2.6598965519291604E-4	0.0	0.006422867850393648	0.0
34-35	1.0952515213825954E-4	2.8163610549838166E-4	0.0	0.012251170589179603	0.0
36-37	1.0952515213825954E-4	2.894593306511145E-4	0.0	0.021247879514822352	0.0
38-39	1.251716024437252E-4	3.3639868156751143E-4	0.0	0.03243509148323029	0.0
40-41	1.251716024437252E-4	3.9898448278937403E-4	0.0	0.04336413702159805	0.0
42-43	1.251716024437252E-4	4.2245415824757255E-4	0.0	0.0553884340813484	0.0
44-45	1.251716024437252E-4	4.2245415824757255E-4	0.0	0.07065936957948288	0.0
46-47	1.251716024437252E-4	4.45923833705771E-4	0.0	0.08866843388107384	0.0
48-49	1.251716024437252E-4	4.693935091639695E-4	0.0	0.10738158844641076	0.0
50-51	1.4081805274919083E-4	4.92863184622168E-4	0.0	0.12625120751480234	0.0
52-53	1.4081805274919083E-4	5.006864097749008E-4	0.0	0.14515994270895755	0.0
54-55	1.4081805274919083E-4	5.006864097749008E-4	0.0	0.16693197830901302	0.0
56-57	1.4081805274919083E-4	5.006864097749008E-4	0.0	0.19281903033940592	0.0
58-59	1.5646450305465648E-4	5.632722109967633E-4	0.0	0.222273473039445	0.0
60-61	1.5646450305465648E-4	5.78918661302229E-4	0.0	0.25215036989773165	0.0
62-63	1.5646450305465648E-4	5.78918661302229E-4	0.0	0.282097675782393	0.0
64-65	1.5646450305465648E-4	5.78918661302229E-4	0.0	0.31549502395940937	0.0
66-67	1.5646450305465648E-4	5.78918661302229E-4	0.0	0.3556438154432342	0.0
68-69	1.5646450305465648E-4	5.78918661302229E-4	0.0	0.40010320398621485	0.0
70-71	1.7993417851285497E-4	6.023883367604274E-4	0.0	0.4481221599736889	0.0
72-73	1.8775740366558778E-4	6.806205882877558E-4	0.0	0.501116687158301	0.0
74-75	1.8775740366558778E-4	6.962670385932214E-4	0.0	0.5557227987243762	0.0
76-77	1.8775740366558778E-4	7.275599392041527E-4	0.0	0.6165249046114156	0.0
78-79	2.0340385397105344E-4	7.353831643568856E-4	0.0	0.6869417542111639	0.0
80-81	2.1905030427651909E-4	7.353831643568856E-4	0.0	0.7643447438723024	0.0
82-83	2.3469675458198474E-4	7.432063895096183E-4	0.0	0.847310046617034	0.0
84-85	2.503432048874504E-4	7.510296146623511E-4	0.0	0.9321059840475052	0.0
86-87	2.738128803456488E-4	7.58852839815084E-4	0.0	1.027596270261762	0.0
88	2.8163610549838166E-4	7.823225152732825E-4	0.0	1.107502691971775	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6465	0.0	44.73451	1
GTATCAA	10735	0.0	35.439087	1
TCAACGC	11590	0.0	31.669302	4
ATCAACG	11700	0.0	31.291464	3
CAACGCA	12500	0.0	29.365847	5
TATCAAC	13170	0.0	28.155647	2
AACGCAG	13295	0.0	27.574936	6
ACGCAGA	14935	0.0	24.10656	7
CGCAGAG	15125	0.0	23.428993	8
GTACATG	18250	0.0	22.933123	1
TACATGG	18095	0.0	21.84297	2
ACATGGG	18550	0.0	20.648474	3
GCAGAGT	17330	0.0	19.444567	9
GTGGTAT	4010	0.0	18.411592	1
AGAGTAC	15420	0.0	17.250954	10-11
CAGAGTA	16895	0.0	17.12186	10-11
CATGGGG	13030	0.0	16.916063	4
TGGTATC	4045	0.0	16.614674	2
GAGTACA	14245	0.0	16.341007	1
GTCTAGG	1975	0.0	14.524421	1
>>END_MODULE