FastQCFastQC Report
Wed 25 May 2016
SRR2049450_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049450_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6391226
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186900.29243215620915297No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178420.27916396635011814No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105220.16463195011410955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112150.056.941231
GTATCAA203400.040.7431561
GTGGTAT48500.032.8930551
ATCAACG255350.032.1593253
TCAACGC256100.032.0834964
TATCAAC266200.031.0781022
CAACGCA266450.030.8196035
AACGCAG277250.029.6487886
TGGTATC51950.029.0448632
ACGCAGA317150.025.8001847
CGCAGAG319900.025.4461678
GCAGAGT349450.022.8102239
GAGTACT251650.021.30036712-13
GTACATG172550.021.0274051
CAGAGTA345250.020.49442510-11
AGAGTAC328950.020.07405910-11
TACATGG176900.019.5834662
GTACTTT272050.019.51309814-15
AGTACTT263950.019.28392812-13
TATAACG6300.018.6530282
Produced by FastQC (version 0.11.5)