FastQCFastQC Report
Wed 25 May 2016
SRR2049449_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049449_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4230869
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56880.13444046601301057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46150.042.695791
GTATCAA71500.033.6091541
ATCAACG77600.029.6749153
TCAACGC78400.029.6115324
CAACGCA85750.027.2407115
TATCAAC89300.026.2079492
AACGCAG92700.025.5551136
GTACCTG32150.023.988641
GTACATG109750.023.0526311
ACGCAGA104200.022.2836467
TACCTGG33400.022.0906412
TACATGG108950.022.041892
CGCAGAG105550.021.6400818
ACATGGG112000.020.686363
GTCTCGC13050.019.8196011
GTGGTAT28700.019.1710851
CATGGGG77250.019.1629354
GCAGAGT121200.018.3031279
CCGATCG1300.00804366218.0764279
TATAACG3501.5152182E-917.4554272
Produced by FastQC (version 0.11.5)