##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049449_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4230869
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.63402577579216	31.0	31.0	34.0	27.0	34.0
2	30.83640831233489	31.0	31.0	34.0	27.0	34.0
3	30.96155305210348	31.0	31.0	34.0	27.0	34.0
4	34.54958969422121	37.0	35.0	37.0	32.0	37.0
5	34.58958715100846	37.0	35.0	37.0	32.0	37.0
6	34.59460834169056	37.0	35.0	37.0	32.0	37.0
7	34.47510877788937	37.0	35.0	37.0	31.0	37.0
8	34.44092431129397	37.0	35.0	37.0	31.0	37.0
9	35.99572144635062	39.0	37.0	39.0	31.0	39.0
10-11	35.92923392333821	39.0	37.0	39.0	30.5	39.0
12-13	35.84164896147813	39.0	37.0	39.0	30.0	39.0
14-15	37.044128523005554	40.0	38.0	41.0	31.0	41.0
16-17	37.031199500622684	40.0	37.0	41.0	31.0	41.0
18-19	37.048271052589904	40.0	37.5	41.0	31.0	41.0
20-21	37.095821213088854	40.0	38.0	41.0	31.0	41.0
22-23	37.0551302817459	40.0	37.0	41.0	31.0	41.0
24-25	37.05832678818465	40.0	37.0	41.0	31.0	41.0
26-27	37.058223145174196	40.0	37.0	41.0	31.0	41.0
28-29	37.034122658962026	40.0	37.0	41.0	31.0	41.0
30-31	36.97616269849054	40.0	37.0	41.0	30.0	41.0
32-33	36.9262795657346	40.0	37.0	41.0	30.0	41.0
34-35	36.85554220657742	40.0	37.0	41.0	30.0	41.0
36-37	36.741185085144444	40.0	37.0	41.0	30.0	41.0
38-39	36.63106645466924	40.0	37.0	41.0	30.0	41.0
40-41	36.511926746018375	40.0	37.0	41.0	30.0	41.0
42-43	36.41485508059928	40.0	36.0	41.0	30.0	41.0
44-45	36.2645403343852	40.0	36.0	41.0	29.5	41.0
46-47	36.139617062121275	40.0	36.0	41.0	29.0	41.0
48-49	36.036073676589844	40.0	36.0	41.0	29.0	41.0
50-51	35.92611033336178	40.0	35.0	41.0	28.0	41.0
52-53	35.81208222235196	39.0	35.0	41.0	28.0	41.0
54-55	35.664890711577215	39.0	35.0	41.0	28.0	41.0
56-57	35.505749291693974	39.0	35.0	41.0	28.0	41.0
58-59	35.32723419231368	39.0	35.0	41.0	27.5	41.0
60-61	35.132429295258255	39.0	35.0	40.5	27.0	41.0
62-63	34.87322947129774	38.0	34.0	40.0	26.5	41.0
64-65	34.62770697934632	38.0	34.0	40.0	26.0	41.0
66-67	34.40279242869491	37.0	34.0	40.0	26.0	41.0
68-69	34.11329445558347	37.0	34.0	40.0	26.0	41.0
70-71	33.79552262194835	36.0	34.0	39.0	26.0	41.0
72-73	33.456204269146596	36.0	33.0	39.0	25.0	41.0
74-75	33.15864542248886	35.5	33.0	39.0	25.0	40.0
76-77	32.83560835847199	35.0	33.0	37.5	25.0	40.0
78-79	32.4902954924863	35.0	33.0	37.0	24.0	39.0
80-81	32.161227752501915	35.0	33.0	37.0	23.5	39.0
82-83	31.87146399474907	35.0	32.5	36.0	23.0	39.0
84-85	31.587774166489204	35.0	32.0	36.0	22.0	37.0
86-87	31.320910786885626	35.0	32.0	35.5	21.0	37.0
88-89	31.094997387061618	35.0	32.0	35.0	20.0	37.0
90-91	30.890865800855572	35.0	32.0	35.0	19.5	36.0
92-93	30.68916468460735	35.0	32.0	35.0	18.0	36.0
94-95	30.48813399800372	35.0	32.0	35.0	13.5	36.0
96-97	30.29931675974841	35.0	32.0	35.0	4.5	35.5
98-99	30.12323165760982	35.0	32.0	35.0	2.0	35.0
100	29.991888664007323	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2807991976853401
1101	2	0.2437572630970024
1101	3	0.31331004448819044
1101	4	0.3827704974222641
1101	5	0.34700007613636075
1101	6	0.33594847468762623
1101	7	0.4186724671294684
1101	8	0.4213989394054991
1101	9	0.5455406209325915
1101	10-11	0.5370224631403389
1101	12-13	0.5668048117501243
1101	14-15	0.6788716100658405
1101	16-17	0.5919538119760404
1101	18-19	0.6279386010167656
1101	20-21	0.5815354562243158
1101	22-23	0.6302596478296536
1101	24-25	0.6223093264222612
1101	26-27	0.6743235295835319
1101	28-29	0.6352953573425495
1101	30-31	0.6392413305909983
1101	32-33	0.6698362477462254
1101	34-35	0.6483018258828324
1101	36-37	0.6217851154573282
1101	38-39	0.6096010774682981
1101	40-41	0.5808740997230757
1101	42-43	0.5939512807926874
1101	44-45	0.5812797931563836
1101	46-47	0.5795291183264908
1101	48-49	0.5548380138278759
1101	50-51	0.49898482875563843
1101	52-53	0.5790174921429099
1101	54-55	0.5756669088768831
1101	56-57	0.5596071733656984
1101	58-59	0.5732707435667308
1101	60-61	0.5412112962530102
1101	62-63	0.4795661417999568
1101	64-65	0.5606022930681789
1101	66-67	0.515772039829038
1101	68-69	0.4712287006318405
1101	70-71	0.47078182136330327
1101	72-73	0.46386635181914215
1101	74-75	0.4200680777961665
1101	76-77	0.3946658316066092
1101	78-79	0.41829147846269166
1101	80-81	0.45777091800184877
1101	82-83	0.41341115569140996
1101	84-85	0.4089490230678763
1101	86-87	0.3097868543694453
1101	88-89	0.3505108697290389
1101	90-91	0.4040283333657584
1101	92-93	0.3425529158524725
1101	94-95	0.2718061055588983
1101	96-97	0.3412304258770149
1101	98-99	0.4021501340471936
1101	100	0.3822496271700544
1102	1	0.1730416301119817
1102	2	0.2682826566796628
1102	3	0.35179590877892863
1102	4	0.32167476782689874
1102	5	0.29763895064268553
1102	6	0.32919149998865294
1102	7	0.27226005523318264
1102	8	0.40842707409943557
1102	9	0.3747325593814921
1102	10-11	0.45882259033271566
1102	12-13	0.42762628579038875
1102	14-15	0.490444942946624
1102	16-17	0.4426754493877354
1102	18-19	0.5440148004199372
1102	20-21	0.517952416379444
1102	22-23	0.49298690129994327
1102	24-25	0.501141676806931
1102	26-27	0.41776270531723014
1102	28-29	0.5234422505772613
1102	30-31	0.43449036792235063
1102	32-33	0.46293626587186054
1102	34-35	0.4683621628270984
1102	36-37	0.38192018634830305
1102	38-39	0.42473893821478725
1102	40-41	0.4270418817301689
1102	42-43	0.3805983739389589
1102	44-45	0.2798726733199288
1102	46-47	0.3316920988454015
1102	48-49	0.2869334119624085
1102	50-51	0.28283117415322323
1102	52-53	0.31032236100646315
1102	54-55	0.291970617507161
1102	56-57	0.23691840858806756
1102	58-59	0.3408029033313298
1102	60-61	0.2986941087932493
1102	62-63	0.17646233125208965
1102	64-65	0.1532688244545355
1102	66-67	0.18707521648514813
1102	68-69	0.10363231882651291
1102	70-71	0.0770507639165885
1102	72-73	0.08767127544474107
1102	74-75	0.17438823164984285
1102	76-77	0.1447616651896766
1102	78-79	0.17331213518842503
1102	80-81	0.1732681501715021
1102	82-83	0.15300401972723776
1102	84-85	0.12814555190027122
1102	86-87	0.07149750759762341
1102	88-89	0.0722919565787592
1102	90-91	0.11377858954381992
1102	92-93	0.11023771730213383
1102	94-95	0.12420222797265623
1102	96-97	0.13813312659677024
1102	98-99	0.06108442970014849
1102	100	0.0960929552390084
1103	1	0.16822930524052282
1103	2	0.20691932758800036
1103	3	0.26142842099186936
1103	4	0.23102916329580125
1103	5	0.28503691986242075
1103	6	0.36596601305225107
1103	7	0.3483966173435036
1103	8	0.3782043776145727
1103	9	0.4433842531200085
1103	10-11	0.4427529636636791
1103	12-13	0.41045862601541927
1103	14-15	0.4645296760027122
1103	16-17	0.4823567118160952
1103	18-19	0.3972447959461647
1103	20-21	0.4306192762713863
1103	22-23	0.4555694644883772
1103	24-25	0.48447918798412104
1103	26-27	0.48505812297685225
1103	28-29	0.4579035727371803
1103	30-31	0.492023078408792
1103	32-33	0.4885987007308614
1103	34-35	0.4458302676004564
1103	36-37	0.4613772059093151
1103	38-39	0.44963254826064514
1103	40-41	0.5208327498919161
1103	42-43	0.43085541288052553
1103	44-45	0.44429604092898956
1103	46-47	0.34133000743997854
1103	48-49	0.3634731360042167
1103	50-51	0.40676150106370557
1103	52-53	0.3991259860983618
1103	54-55	0.33463354036153703
1103	56-57	0.31009121117275384
1103	58-59	0.31655887547207584
1103	60-61	0.3677660384987078
1103	62-63	0.42109056697763947
1103	64-65	0.46891783799016906
1103	66-67	0.4259775431379893
1103	68-69	0.40730728373551983
1103	70-71	0.4078205838689257
1103	72-73	0.4209855328591132
1103	74-75	0.44565518310619723
1103	76-77	0.36718854857038963
1103	78-79	0.4124731545126963
1103	80-81	0.43109610946305565
1103	82-83	0.35904735789410225
1103	84-85	0.3513855046210068
1103	86-87	0.36541387064529474
1103	88-89	0.33046006199040434
1103	90-91	0.33734284739337994
1103	92-93	0.25472062788989547
1103	94-95	0.32570288627319144
1103	96-97	0.37503911118400524
1103	98-99	0.30453465852413686
1103	100	0.26398753083100246
1104	1	0.2227403415981435
1104	2	0.20458700205987768
1104	3	0.2713809634321507
1104	4	0.2760700616300795
1104	5	0.3105857139135111
1104	6	0.352382140918003
1104	7	0.3575430813058702
1104	8	0.3329677173642196
1104	9	0.4751075420361772
1104	10-11	0.4657224308524306
1104	12-13	0.4941617985928488
1104	14-15	0.5066209318738117
1104	16-17	0.47887582801276807
1104	18-19	0.5146792875720223
1104	20-21	0.4919913411370942
1104	22-23	0.577778831981874
1104	24-25	0.5334190456652905
1104	26-27	0.5235895855122266
1104	28-29	0.4863887126845867
1104	30-31	0.4763572372891005
1104	32-33	0.4159839950592783
1104	34-35	0.5116185324067644
1104	36-37	0.5567745226538321
1104	38-39	0.5290761387147853
1104	40-41	0.5484741170441012
1104	42-43	0.5719312832568093
1104	44-45	0.52441502242025
1104	46-47	0.4904331933554005
1104	48-49	0.5312392340666037
1104	50-51	0.4874771297489957
1104	52-53	0.4674017858909991
1104	54-55	0.41976230373525425
1104	56-57	0.38022824037177116
1104	58-59	0.38891502298029224
1104	60-61	0.399144652234277
1104	62-63	0.5004251752901965
1104	64-65	0.5401661039480175
1104	66-67	0.4091710968298159
1104	68-69	0.43749703527198136
1104	70-71	0.41379281351264297
1104	72-73	0.4101498664132208
1104	74-75	0.41064354136127434
1104	76-77	0.34620068406945137
1104	78-79	0.3283988378878888
1104	80-81	0.3696150624557575
1104	82-83	0.4022942966806049
1104	84-85	0.3555539990478387
1104	86-87	0.3081615047055486
1104	88-89	0.2751827540108458
1104	90-91	0.29335999955042524
1104	92-93	0.3201646317388551
1104	94-95	0.30824250569313705
1104	96-97	0.3819051932159532
1104	98-99	0.4126320606400675
1104	100	0.3577393456466922
1105	1	0.0491577122703859
1105	2	0.0107783142019926
1105	3	0.11786436174345027
1105	4	0.06316196889239478
1105	5	0.1710721876056951
1105	6	0.05611739917610237
1105	7	0.07721333041938294
1105	8	0.14948852342833163
1105	9	0.11205804700333744
1105	10-11	0.13966906661526224
1105	12-13	0.16863362882008204
1105	14-15	0.1779574494388214
1105	16-17	0.1866249062789933
1105	18-19	0.20714674578509573
1105	20-21	0.19046249814926597
1105	22-23	0.13175329332869978
1105	24-25	0.14160772208585826
1105	26-27	0.15667558120788527
1105	28-29	0.1333945230082918
1105	30-31	0.1527997271718533
1105	32-33	0.15163360529520276
1105	34-35	0.1182885380158254
1105	36-37	0.12887156468391936
1105	38-39	0.15292423417959355
1105	40-41	0.23543015233410358
1105	42-43	0.1755517374391644
1105	44-45	0.15450591868953722
1105	46-47	0.13281321713763816
1105	48-49	0.15264297471419752
1105	50-51	0.18965115578838265
1105	52-53	0.12479462783905859
1105	54-55	0.08768982548932058
1105	56-57	0.05974104366768529
1105	58-59	-0.012548177017201567
1105	60-61	0.022983863005329397
1105	62-63	0.11570635110865624
1105	64-65	0.04449912120969657
1105	66-67	0.028495606332207046
1105	68-69	0.018135367001015368
1105	70-71	0.052858261305736676
1105	72-73	0.07530656075597619
1105	74-75	0.04514446443838693
1105	76-77	0.012420256453928857
1105	78-79	0.0345940922803436
1105	80-81	-0.010344398867921711
1105	82-83	-0.08229538821123228
1105	84-85	-0.07958647301456168
1105	86-87	-0.096122465854517
1105	88-89	-0.11674916465579344
1105	90-91	-0.1402018566185106
1105	92-93	-0.14863736466779187
1105	94-95	-0.1742144137834991
1105	96-97	-0.14951393018824177
1105	98-99	-0.1438770788103021
1105	100	-0.2320123941971346
1106	1	0.16483110593747696
1106	2	0.15313247351739534
1106	3	0.17661331004641312
1106	4	0.24693364980860366
1106	5	0.2582681348819875
1106	6	0.22557737832360658
1106	7	0.2605311826693679
1106	8	0.24129770485607338
1106	9	0.30044386288216884
1106	10-11	0.31206957652586453
1106	12-13	0.28762227782724636
1106	14-15	0.43163299063974137
1106	16-17	0.39780771766946543
1106	18-19	0.40289356585733316
1106	20-21	0.3613022626309004
1106	22-23	0.3396701880936206
1106	24-25	0.3780290618391433
1106	26-27	0.39052874692856676
1106	28-29	0.3030071639112464
1106	30-31	0.32759515939005723
1106	32-33	0.3449092558288598
1106	34-35	0.31719605996923406
1106	36-37	0.2939612841893364
1106	38-39	0.35721527391386587
1106	40-41	0.3820161680900611
1106	42-43	0.3145924301680978
1106	44-45	0.2258143494842031
1106	46-47	0.2729381905315478
1106	48-49	0.21673456970846416
1106	50-51	0.2146506217360553
1106	52-53	0.2006351168302487
1106	54-55	0.18913398021573613
1106	56-57	0.1843888972791774
1106	58-59	0.23995831718788452
1106	60-61	0.22150119216713193
1106	62-63	0.24108038720852676
1106	64-65	0.19126764130821527
1106	66-67	0.25024166406082315
1106	68-69	0.23739110651936812
1106	70-71	0.2959589485731797
1106	72-73	0.26085716356593736
1106	74-75	0.22858643559853675
1106	76-77	0.14233348002728263
1106	78-79	0.18928812919352112
1106	80-81	0.22610581831765586
1106	82-83	0.24452415383181503
1106	84-85	0.2257930039847693
1106	86-87	0.2561488780296308
1106	88-89	0.18860097163693368
1106	90-91	0.22653265399780764
1106	92-93	0.14715593726069187
1106	94-95	0.1994482998663898
1106	96-97	0.18017147824082969
1106	98-99	0.19992971572522222
1106	100	0.18980306459757657
1107	1	0.04011343410528667
1107	2	0.09715414885680573
1107	3	0.1456857979351689
1107	4	0.04844400643946756
1107	5	0.11039609221750624
1107	6	0.06935682878563654
1107	7	0.0724641562518542
1107	8	-0.01832793757405682
1107	9	0.021959591698646364
1107	10-11	0.06608532289850899
1107	12-13	0.06252032668553653
1107	14-15	0.13101962849423643
1107	16-17	0.07658515168087376
1107	18-19	0.09039173500042352
1107	20-21	0.17782219740223582
1107	22-23	0.10379896103827235
1107	24-25	0.09030493234890713
1107	26-27	0.1364466633431789
1107	28-29	0.027534238522925136
1107	30-31	0.007708830524016719
1107	32-33	0.03489761609334607
1107	34-35	-0.007523066516391452
1107	36-37	-0.02055959309710431
1107	38-39	0.04719633583093241
1107	40-41	0.09064612532325356
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2216	92-93	-1.0676865494204186
2216	94-95	-1.0316242199344359
2216	96-97	-1.1225886504511635
2216	98-99	-1.1928818256316625
2216	100	-1.2711891219600027
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	82238.0
3	15154.0
4	9569.0
5	7784.0
6	6441.0
7	6144.0
8	6382.0
9	7106.0
10	8250.0
11	9449.0
12	10697.0
13	11922.0
14	12852.0
15	13580.0
16	13719.0
17	14734.0
18	14425.0
19	13064.0
20	12816.0
21	13810.0
22	16062.0
23	19607.0
24	24281.0
25	28859.0
26	35148.0
27	39708.0
28	47773.0
29	59405.0
30	71315.0
31	82306.0
32	104502.0
33	138859.0
34	191792.0
35	277633.0
36	456539.0
37	827147.0
38	1109983.0
39	408902.0
40	912.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.565669568031154	20.065539709144478	13.549911464150668	22.8188792586737
2	16.066314558013655	20.947738506019515	39.72103312205711	23.26491381390972
3	18.041007296626567	26.06159935737868	30.64528787704714	25.25210546894761
4	12.779673172626227	18.004817005556646	38.90083015221388	30.31467966960325
5	12.73520213874923	37.38239597589889	35.03000820232076	14.852393683031123
6	29.149723596407025	38.41318549261107	18.644590623196912	13.792500287784987
7	25.86947944353126	32.88980624491877	23.118435775056966	18.122278536492995
8	24.016383929320668	35.77959227861946	21.667741798993827	18.536281993066048
9	24.16056632535957	17.972788565704157	22.46133495772723	35.405310151209044
10-11	22.920639730970038	27.458038235736655	29.41445537259153	20.206866660701774
12-13	23.700887468636488	25.34212055408755	29.118672867742067	21.838319109533895
14-15	21.463516220561484	27.721292435069238	26.43421849952789	24.38097284484139
16-17	20.774460483481406	30.713298711202423	26.863583138796105	21.648657666520066
18-19	20.622358480754137	29.516005124665522	28.979924453731453	20.88171194084888
20-21	21.954118454537515	29.09440486168083	28.237563873917882	20.71391280986378
22-23	21.628935027290673	29.167930862447662	28.1873955967337	21.015738513527968
24-25	21.356401588577373	29.021334688667206	28.352607441728523	21.2696562810269
26-27	21.134868566840286	28.84076755608508	28.46857875335188	21.555785123722757
28-29	21.247064521836627	28.892385834439327	28.50156789312301	21.358981750601043
30-31	21.59523745081314	29.46041927008017	27.967601825207332	20.976741453899354
32-33	21.010593912471627	29.101595280205867	28.54100389707339	21.346806910249118
34-35	21.196956816133113	28.55791278744953	28.62323598124904	21.621894415168313
36-37	21.79029174040803	29.06729065234128	27.96471004545864	21.17770756179205
38-39	21.520377933754265	29.158118212925675	28.157102504731913	21.164401348588143
40-41	21.28003040892487	28.88397543741787	28.400680607715177	21.435313545942083
42-43	21.13153702482646	28.920838005208072	28.302537272666783	21.64508769729868
44-45	21.450028676082255	29.11724002282739	28.10444004098339	21.328291260106962
46-47	21.396739256986137	29.387348010731163	27.873912011024775	21.342000721257925
48-49	21.2473329666772	29.005112500905707	28.219721348429356	21.52783318398774
50-51	21.314498978885222	28.82694660383453	28.215294182582817	21.643260234697433
52-53	21.31815535405764	28.900144122567294	28.07773305153357	21.703967471841494
54-55	21.507776544503137	29.35062712429127	27.88091334079444	21.260682990411148
56-57	21.21532164417345	29.092313126002363	27.930426267891185	21.761938961933005
58-59	21.087605209581255	28.658653253541928	28.373634989185803	21.88010654769101
60-61	21.552409623460438	29.178920705397704	27.85423173854731	21.414437932594556
62-63	21.351363969734344	29.16089813561155	27.890227607038188	21.597510287615915
64-65	21.375427397786545	28.766464234525763	28.179712457703506	21.67839590998419
66-67	21.249009093938074	28.90768614525693	27.90724776498752	21.93605699581748
68-69	21.716046766353916	29.12311490761746	27.804745496350286	21.356092829678342
70-71	21.757981672653752	28.782581774857174	27.933404408412265	21.526032144076808
72-73	21.37478407297181	28.95029736287112	28.309543436823066	21.365375127334005
74-75	21.34484395122231	28.96525597176837	28.11285795592561	21.577042121083707
76-77	21.508507487153643	29.006168213319555	27.777028585057558	21.708295714469248
78-79	21.64747769823151	29.199821670950755	27.890779130584427	21.261921500233314
80-81	21.406706501692245	28.8923398761167	28.089508595625635	21.611445026565416
82-83	21.257539243067676	28.958612318322412	28.225259522523473	21.55858891608644
84-85	21.43345952328329	28.88867351630285	28.00972486020809	21.668142100205774
86-87	21.570667892470468	29.138509170893368	27.86093021062258	21.429892726013584
88-89	21.28055809579666	29.00737463806735	27.988508457495865	21.723558808640124
90-91	21.154933566197172	28.896824897771417	28.02949535083941	21.918746185192
92-93	21.39641423103388	29.15487251442304	27.855528243680443	21.593185010862637
94-95	21.297845336139805	28.918999229117926	28.058192731865656	21.724962702876613
96-97	21.273745232751416	29.000567690704422	28.047289356668024	21.678397719876134
98-99	21.2197000892102	29.000985236113912	28.014153550277538	21.765161124398354
100	21.42438255080673	29.08669261575983	27.816089997827724	21.672834835605713
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5194.0
1	3843.5
2	2952.5
3	3355.0
4	2951.0
5	2368.0
6	2048.5
7	1907.0
8	1886.5
9	1893.5
10	1660.5
11	1455.0
12	1456.0
13	1468.0
14	1507.0
15	1676.5
16	1919.5
17	2239.5
18	2726.0
19	3443.0
20	4517.0
21	6199.0
22	9071.5
23	13351.5
24	18741.5
25	25613.5
26	34572.0
27	44739.0
28	57154.5
29	71059.0
30	82694.5
31	93346.5
32	103844.0
33	112575.5
34	121328.5
35	128839.5
36	134255.5
37	140111.0
38	145877.0
39	149253.5
40	150490.0
41	150432.0
42	147521.0
43	143357.0
44	140195.5
45	138165.0
46	136889.0
47	134143.5
48	130886.5
49	129368.0
50	126623.5
51	121425.0
52	113973.0
53	107327.5
54	102747.5
55	97767.5
56	90815.0
57	82647.5
58	75385.0
59	68843.5
60	60465.5
61	50628.5
62	41842.5
63	33932.5
64	27134.0
65	21311.5
66	16533.0
67	12564.5
68	9306.0
69	6865.5
70	5154.0
71	3822.5
72	2812.5
73	2037.0
74	1442.5
75	1017.0
76	659.0
77	435.5
78	332.5
79	245.5
80	164.0
81	109.0
82	94.0
83	83.0
84	70.0
85	59.5
86	44.5
87	29.0
88	28.5
89	25.0
90	17.0
91	15.0
92	13.5
93	10.5
94	7.0
95	4.5
96	5.0
97	4.5
98	6.0
99	8.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07570548745423222
2	0.012905150218548482
3	0.016426885351449077
4	0.010399754754874235
5	0.008485254447726933
6	0.006618025753101785
7	0.0198068056467832
8	0.009028877991731722
9	0.008697976704076633
10-11	0.013224233603073034
12-13	0.015008736975784408
14-15	0.009938856532783218
16-17	0.012538795221501777
18-19	0.008248896385116155
20-21	0.013295141021856267
22-23	0.013649678115772433
24-25	0.015812354388661052
26-27	0.0052116952805676565
28-29	0.01206607909628022
30-31	0.010529751689310163
32-33	0.01217244022445507
34-35	0.008638887188423938
36-37	0.010163396692263457
38-39	0.01927500000590895
40-41	0.012574248930893392
42-43	0.007622547519197593
44-45	0.020397700803310145
46-47	0.017951394855288592
48-49	0.01766776518015566
50-51	0.010482480076788007
52-53	0.010447026367396391
54-55	0.011522455552275431
56-57	0.02016134274069937
58-59	0.002068133047844308
60-61	0.006878019621973643
62-63	0.015635085841702968
64-65	0.024971229314828702
66-67	0.012373344577674231
68-69	0.014311480691082613
70-71	0.025278494796222715
72-73	0.020137706934438292
74-75	0.020362247093918533
76-77	0.009206146538689806
78-79	0.011510637649144891
80-81	0.01984225935617482
82-83	0.011912446355583214
84-85	0.01120337216775088
86-87	0.017005962604845482
88-89	0.014831468428826324
90-91	0.015197823425873029
92-93	0.007374371553456276
94-95	0.015363274069700575
96-97	0.013531499084467045
98-99	0.009714316373302978
100	0.006570754140579631
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4230869.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.72683485806084
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.85769176298875	54.085087021778655
2	12.949253121797888	17.54023855430469
3	3.46208396953378	7.034279678080624
4	1.2892886684935705	3.4927776294173283
5	0.6789037453544814	2.299000092307098
6	0.41226189707298133	1.675271605279961
7	0.2723430982711968	1.2911455228942188
8	0.19523439884414515	1.0578086311304165
9	0.1493445457227702	0.9103170046606357
>10	0.6944362573798178	7.946146038292758
>50	0.029159854230291868	1.3321233787660647
>100	0.009754424244218005	1.0806220525228025
>500	1.3957491004324728E-4	0.06139701843161119
>1k	6.9787437388069E-5	0.05936461702419149
>5k	3.489371869403311E-5	0.1344211551089125
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5688	0.13444046601301057	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.727161252215561E-5	0.0
4	0.0	0.0	0.0	1.1817903130538903E-4	0.0
5	0.0	2.3635806261077806E-5	0.0	1.4181483756646685E-4	0.0
6	0.0	2.3635806261077806E-5	0.0	1.4181483756646685E-4	0.0
7	0.0	2.3635806261077806E-5	0.0	1.4181483756646685E-4	0.0
8	0.0	2.3635806261077806E-5	0.0	1.4181483756646685E-4	0.0
9	0.0	2.3635806261077806E-5	0.0	1.6545064382754465E-4	0.0
10-11	0.0	2.3635806261077806E-5	0.0	1.7726854695808355E-4	0.0
12-13	0.0	2.3635806261077806E-5	0.0	2.0090435321916134E-4	0.0
14-15	0.0	4.727161252215561E-5	0.0	2.3635806261077807E-4	0.0
16-17	0.0	7.090741878323342E-5	0.0	2.3635806261077807E-4	0.0
18-19	0.0	7.090741878323342E-5	0.0	2.718117720023948E-4	0.0
20-21	0.0	7.090741878323342E-5	0.0	3.309012876550893E-4	0.0
22-23	0.0	7.090741878323342E-5	0.0	4.6089822209101724E-4	0.0
24-25	0.0	7.090741878323342E-5	0.0	5.199877377437118E-4	0.0
26-27	0.0	7.090741878323342E-5	0.0	8.981606379209567E-4	0.0
28-29	0.0	7.090741878323342E-5	0.0	0.001713595953928141	0.0
30-31	0.0	7.090741878323342E-5	0.0	0.0037344573892502935	0.0
32-33	0.0	7.090741878323342E-5	0.0	0.008000720419374838	0.0
34-35	0.0	7.090741878323342E-5	0.0	0.015469635197875423	0.0
36-37	0.0	7.090741878323342E-5	0.0	0.02552667076196403	0.0
38-39	0.0	7.090741878323342E-5	0.0	0.03808910178972689	0.0
40-41	0.0	9.454322504431122E-5	0.0	0.052601486834028655	0.0
42-43	0.0	1.1817903130538903E-4	0.0	0.06823657267573163	0.0
44-45	0.0	1.2999693443592795E-4	0.0	0.08669613736563339	0.0
46-47	0.0	1.4181483756646685E-4	0.0	0.1082047210632142	0.0
48-49	0.0	1.5363274069700575E-4	0.0	0.13018602088601655	0.0
50-51	0.0	1.8908645008862244E-4	0.0	0.1529236665091734	0.0
52-53	0.0	2.0090435321916134E-4	0.0	0.1753422287478057	0.0
54-55	0.0	2.1272225634970027E-4	0.0	0.20135343353812185	0.0
56-57	0.0	2.1272225634970027E-4	0.0	0.23239906506204755	0.0
58-59	0.0	2.1272225634970027E-4	0.0	0.26507556721798764	0.0
60-61	0.0	2.3635806261077807E-4	0.0	0.3008601778972594	0.0
62-63	0.0	2.3635806261077807E-4	0.0	0.3375784029238438	0.0
64-65	0.0	2.3635806261077807E-4	0.0	0.37745200808628204	0.0
66-67	2.3635806261077806E-5	2.3635806261077807E-4	0.0	0.4250427039929622	0.0
68-69	2.3635806261077806E-5	2.3635806261077807E-4	0.0	0.4769469345422891	0.0
70-71	2.3635806261077806E-5	2.3635806261077807E-4	0.0	0.5342992184347943	1.1817903130538903E-5
72-73	2.3635806261077806E-5	2.3635806261077807E-4	0.0	0.5968395618016062	2.3635806261077806E-5
74-75	2.3635806261077806E-5	2.3635806261077807E-4	0.0	0.6619562080508756	2.3635806261077806E-5
76-77	2.3635806261077806E-5	2.3635806261077807E-4	0.0	0.7345535869817761	2.3635806261077806E-5
78-79	2.3635806261077806E-5	2.48175965741317E-4	0.0	0.8167471032546743	2.3635806261077806E-5
80-81	2.3635806261077806E-5	2.836296751329337E-4	0.0	0.907685867844171	2.3635806261077806E-5
82-83	2.3635806261077806E-5	2.954475782634726E-4	0.0	1.0051717507679865	2.3635806261077806E-5
84-85	2.3635806261077806E-5	3.309012876550893E-4	0.0	1.1073493412346256	2.3635806261077806E-5
86-87	2.3635806261077806E-5	3.309012876550893E-4	0.0	1.2188158035618688	2.3635806261077806E-5
88	2.3635806261077806E-5	3.309012876550893E-4	0.0	1.3154980690728075	2.3635806261077806E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4615	0.0	42.69579	1
GTATCAA	7150	0.0	33.609154	1
ATCAACG	7760	0.0	29.674915	3
TCAACGC	7840	0.0	29.611532	4
CAACGCA	8575	0.0	27.240711	5
TATCAAC	8930	0.0	26.207949	2
AACGCAG	9270	0.0	25.555113	6
GTACCTG	3215	0.0	23.98864	1
GTACATG	10975	0.0	23.052631	1
ACGCAGA	10420	0.0	22.283646	7
TACCTGG	3340	0.0	22.090641	2
TACATGG	10895	0.0	22.04189	2
CGCAGAG	10555	0.0	21.640081	8
ACATGGG	11200	0.0	20.68636	3
GTCTCGC	1305	0.0	19.819601	1
GTGGTAT	2870	0.0	19.171085	1
CATGGGG	7725	0.0	19.162935	4
GCAGAGT	12120	0.0	18.303127	9
CCGATCG	130	0.008043662	18.076427	9
TATAACG	350	1.5152182E-9	17.455427	2
>>END_MODULE