FastQCFastQC Report
Wed 25 May 2016
SRR2049449_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049449_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4230869
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182600.43158982232728077No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176660.4175501534082005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109240.258197547596014No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70580.16682152059068717No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47830.11305006134673516No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45900.10848835073834714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90550.058.113531
TATAACG4050.039.4613952
GTATCAA157600.038.7353671
GTGGTAT34100.032.0226671
ATCAACG198100.030.348323
TCAACGC200300.030.0384584
TATCAAC204100.029.5943432
ATAACGC4800.029.3784333
CAACGCA208300.028.8847945
TGGTATC38450.028.117722
GTACCTG32650.027.6784611
AACGCAG218950.027.6476296
TACCTGG33050.026.1694722
ACGCAGA248700.024.208087
CGCAGAG251450.023.980718
TAACGCA6700.023.1519574
GCAGAGT274600.021.7365289
GCGGTAT3351.8189894E-1221.0751321
CAGAGTA267800.020.44571710-11
GAGTACT217250.020.24897612-13
Produced by FastQC (version 0.11.5)