##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049449_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4230869 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.771586168231632 31.0 31.0 34.0 30.0 34.0 2 31.932980907704778 33.0 31.0 34.0 30.0 34.0 3 32.07447642552866 33.0 31.0 34.0 30.0 34.0 4 35.823875898781075 37.0 35.0 37.0 35.0 37.0 5 35.8425964027721 37.0 35.0 37.0 35.0 37.0 6 35.94920641598688 37.0 35.0 37.0 35.0 37.0 7 35.93164264835428 37.0 35.0 37.0 35.0 37.0 8 35.95069570813939 37.0 35.0 37.0 35.0 37.0 9 37.66734635366871 39.0 37.0 39.0 35.0 39.0 10-11 37.63867068443859 39.0 37.0 39.0 35.0 39.0 12-13 37.61500982422287 39.0 37.0 39.0 35.0 39.0 14-15 38.91704600166065 40.0 38.0 41.0 35.0 41.0 16-17 38.92338583397406 40.0 38.0 41.0 35.5 41.0 18-19 39.00428824432995 40.0 38.5 41.0 36.0 41.0 20-21 39.02137031423095 40.0 39.0 41.0 35.0 41.0 22-23 38.973739910169755 40.0 39.0 41.0 35.0 41.0 24-25 38.94095432876792 40.0 38.0 41.0 35.0 41.0 26-27 38.83657742652869 40.0 38.0 41.0 35.0 41.0 28-29 38.74158819382023 40.0 38.0 41.0 35.0 41.0 30-31 38.60826121064018 40.0 38.0 41.0 35.0 41.0 32-33 38.439688985879734 40.0 38.0 41.0 34.0 41.0 34-35 38.29499034359135 40.0 38.0 41.0 34.0 41.0 36-37 38.14137190255713 40.0 38.0 41.0 34.0 41.0 38-39 38.01918601119534 40.0 38.0 41.0 34.0 41.0 40-41 37.918868322323384 40.0 38.0 41.0 33.0 41.0 42-43 37.76335133515124 40.0 37.5 41.0 33.0 41.0 44-45 37.57144844238855 40.0 37.0 41.0 33.0 41.0 46-47 37.29432204116932 40.0 37.0 41.0 32.5 41.0 48-49 37.018944571434375 40.0 37.0 41.0 31.5 41.0 50-51 36.8534122658962 40.0 37.0 41.0 31.0 41.0 52-53 36.73486049792608 40.0 36.5 41.0 31.0 41.0 54-55 36.591505551223634 40.0 36.0 41.0 31.0 41.0 56-57 36.44664429458818 39.5 36.0 41.0 31.0 41.0 58-59 36.275980891868784 39.0 35.0 41.0 30.5 41.0 60-61 36.08572777838312 39.0 35.0 41.0 30.0 41.0 62-63 35.85971073554866 39.0 35.0 41.0 30.0 41.0 64-65 35.62910858265761 38.5 35.0 40.5 29.5 41.0 66-67 35.36665540814428 38.0 35.0 40.0 29.0 41.0 68-69 35.06971735593799 37.0 35.0 40.0 29.0 41.0 70-71 34.775110621482256 37.0 34.5 39.5 29.0 41.0 72-73 34.44034428860832 36.0 34.0 39.0 28.5 41.0 74-75 34.117995262911705 36.0 34.0 39.0 28.0 41.0 76-77 33.79959778948486 35.0 34.0 38.5 28.0 40.0 78-79 33.492369534485704 35.0 34.0 37.0 28.0 39.0 80-81 33.18673516008177 35.0 34.0 37.0 27.5 39.0 82-83 32.910822575693075 35.0 34.0 36.5 27.0 39.0 84-85 32.627608536213245 35.0 34.0 36.0 27.0 37.5 86-87 32.382522124887345 35.0 33.5 36.0 26.5 37.0 88-89 32.16432297951083 35.0 33.0 35.0 26.0 37.0 90-91 31.976481900054104 35.0 33.0 35.0 26.0 36.0 92-93 31.80965931585213 35.0 33.0 35.0 26.0 36.0 94-95 31.645171476592637 35.0 33.0 35.0 25.0 36.0 96-97 31.495590503983934 35.0 33.0 35.0 25.0 36.0 98-99 31.360766665193367 35.0 33.0 35.0 25.0 35.5 100 31.252378412094536 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2237757694191025 1101 2 0.25686176397804417 1101 3 0.40029555962063057 1101 4 0.27497456735225967 1101 5 0.26164815129706653 1101 6 0.2384985004913105 1101 7 0.24221192793241642 1101 8 0.22455660453014303 1101 9 0.28537259007836724 1101 10-11 0.299211510920415 1101 12-13 0.27171486029137526 1101 14-15 0.39548485802389877 1101 16-17 0.35133957805317095 1101 18-19 0.35961596309357446 1101 20-21 0.3924900066907284 1101 22-23 0.3844092069895595 1101 24-25 0.3319657135189331 1101 26-27 0.4316864006444163 1101 28-29 0.38699248553425747 1101 30-31 0.3979448496745235 1101 32-33 0.39154009749285024 1101 34-35 0.4375381456501728 1101 36-37 0.49446880573044893 1101 38-39 0.49083306389211856 1101 40-41 0.5151958869173043 1101 42-43 0.4672347665669818 1101 44-45 0.5004682362822024 1101 46-47 0.551389369372977 1101 48-49 0.4969274792264571 1101 50-51 0.5168742675850666 1101 52-53 0.481640094617255 1101 54-55 0.4719186828236275 1101 56-57 0.49905790625437874 1101 58-59 0.45397275578771 1101 60-61 0.3328360689448786 1101 62-63 0.4118959577989756 1101 64-65 0.42456820572691356 1101 66-67 0.44930973291968 1101 68-69 0.467698413714146 1101 70-71 0.42648060576757274 1101 72-73 0.48826597330680954 1101 74-75 0.4142369272041364 1101 76-77 0.4352830628078834 1101 78-79 0.34320844180511045 1101 80-81 0.2731313818054133 1101 82-83 0.3502488223280693 1101 84-85 0.2945809699918982 1101 86-87 0.21516711748138562 1101 88-89 0.23389087810442533 1101 90-91 0.263304903529189 1101 92-93 0.29142887821692653 1101 94-95 0.28340488572613154 1101 96-97 0.2804550704065534 1101 98-99 0.33873364759401525 1101 100 0.35766518168249206 1102 1 0.12711636263529869 1102 2 0.1574316984826325 1102 3 0.2751870284639111 1102 4 0.193574575006906 1102 5 0.16742294761233723 1102 6 0.18525233870905566 1102 7 0.21790991536096982 1102 8 0.18039973906640938 1102 9 0.223111752789535 1102 10-11 0.24136268931542304 1102 12-13 0.19653968793534915 1102 14-15 0.22516189192847236 1102 16-17 0.23546483077099367 1102 18-19 0.2777429710854733 1102 20-21 0.30437323859992915 1102 22-23 0.2819398779486022 1102 24-25 0.2985090813294917 1102 26-27 0.23361198467863886 1102 28-29 0.24338328868768144 1102 30-31 0.24888983800840236 1102 32-33 0.35831214198515227 1102 34-35 0.34809014829222207 1102 36-37 0.3536376694986032 1102 38-39 0.29487550195143086 1102 40-41 0.3129523941529939 1102 42-43 0.2689423856166684 1102 44-45 0.3621803759088138 1102 46-47 0.35766453433913625 1102 48-49 0.4283030212202803 1102 50-51 0.3016488597763214 1102 52-53 0.3187224617916016 1102 54-55 0.27257499091803084 1102 56-57 0.29059106786355926 1102 58-59 0.31362436806664107 1102 60-61 0.29786986976555596 1102 62-63 0.24628592784714698 1102 64-65 0.22988115337656012 1102 66-67 0.23737104089734373 1102 68-69 0.24451114580593014 1102 70-71 0.22229152577718736 1102 72-73 0.2713881357601693 1102 74-75 0.2500967166242063 1102 76-77 0.2221157451348148 1102 78-79 0.2213889733797103 1102 80-81 0.23872176963384106 1102 82-83 0.19415378839124742 1102 84-85 0.11253799297776368 1102 86-87 0.12880369775881917 1102 88-89 0.13070380517316238 1102 90-91 0.15877264001758817 1102 92-93 0.16605432988346536 1102 94-95 0.07671985993260577 1102 96-97 0.08875576917027672 1102 98-99 0.14529709562435045 1102 100 0.1734010018627039 1103 1 0.10049255948601754 1103 2 0.14418421984041174 1103 3 0.2541841370478153 1103 4 0.14654214971633195 1103 5 0.17736757957620597 1103 6 0.13294886709489617 1103 7 0.1538660352349197 1103 8 0.12367854573494697 1103 9 0.2072143026088682 1103 10-11 0.18906084241832133 1103 12-13 0.15447045409968752 1103 14-15 0.2434308777626839 1103 16-17 0.2564011378007365 1103 18-19 0.2223705830357261 1103 20-21 0.26458058150864616 1103 22-23 0.2567079786021509 1103 24-25 0.24167327291380758 1103 26-27 0.2992260813040488 1103 28-29 0.3090100203894508 1103 30-31 0.32783359204481144 1103 32-33 0.2949154649957251 1103 34-35 0.327612519195398 1103 36-37 0.29718365508388445 1103 38-39 0.268098772309564 1103 40-41 0.24296511046521374 1103 42-43 0.258544327037491 1103 44-45 0.24598950472972092 1103 46-47 0.23750260807459256 1103 48-49 0.2469922720342339 1103 50-51 0.24045564726390012 1103 52-53 0.24565635379550343 1103 54-55 0.14746836886145331 1103 56-57 0.14128369054565582 1103 58-59 0.17852221108067567 1103 60-61 0.1380605100027239 1103 62-63 0.2088319203954896 1103 64-65 0.15546564533624263 1103 66-67 0.14336717523126907 1103 68-69 0.13596007137473975 1103 70-71 0.15311229221425293 1103 72-73 0.17218507398088434 1103 74-75 0.13324990945432802 1103 76-77 0.1898260168861441 1103 78-79 0.14928334878334937 1103 80-81 0.10308544339761738 1103 82-83 0.10875528011359847 1103 84-85 0.08019192186273472 1103 86-87 0.05868143199804621 1103 88-89 0.12219970247247858 1103 90-91 0.11574540337696959 1103 92-93 0.1810603181263133 1103 94-95 0.1585887139326303 1103 96-97 0.18564678605167018 1103 98-99 0.20781300189442575 1103 100 0.2307641905323905 1104 1 0.1799631987195056 1104 2 0.20637338152293694 1104 3 0.3110923662971956 1104 4 0.23568780180109883 1104 5 0.17258139541337414 1104 6 0.20516231468757695 1104 7 0.19142738687857985 1104 8 0.19080715418915872 1104 9 0.2556149658631668 1104 10-11 0.2435462383557052 1104 12-13 0.2246541248385867 1104 14-15 0.31153970415632415 1104 16-17 0.27678966265640526 1104 18-19 0.2923121816972909 1104 20-21 0.2746578051523514 1104 22-23 0.2702587542973518 1104 24-25 0.23821923077819207 1104 26-27 0.28102772055120084 1104 28-29 0.27284435262220086 1104 30-31 0.30795104090998393 1104 32-33 0.24715904318885862 1104 34-35 0.20894202676263518 1104 36-37 0.21585766841441512 1104 38-39 0.24144222097508106 1104 40-41 0.2738885756748104 1104 42-43 0.2263460897231866 1104 44-45 0.24072464415935002 1104 46-47 0.22847266511728037 1104 48-49 0.19097184751338148 1104 50-51 0.18582295353272116 1104 52-53 0.17736054701905601 1104 54-55 0.16168782517684122 1104 56-57 0.18722970814103235 1104 58-59 0.18539363659276376 1104 60-61 0.17032597575329333 1104 62-63 0.2024133237590675 1104 64-65 0.18041787503796058 1104 66-67 0.18183302480111507 1104 68-69 0.17297829347048577 1104 70-71 0.11522449121373768 1104 72-73 0.133931928058729 1104 74-75 0.11391711543969052 1104 76-77 0.10128330818907472 1104 78-79 0.10964996951265249 1104 80-81 0.11956610310616611 1104 82-83 0.18956100235950402 1104 84-85 0.1538491446531367 1104 86-87 0.13087416842736133 1104 88-89 0.16435619850815186 1104 90-91 0.19757951772827198 1104 92-93 0.20019884142865152 1104 94-95 0.15641346186741956 1104 96-97 0.10834796083027598 1104 98-99 0.19176822932992366 1104 100 0.13113048816754969 1105 1 0.08960487902045244 1105 2 0.12553189887716343 1105 3 0.20954401422434188 1105 4 0.12321212764037881 1105 5 0.1274815992878331 1105 6 0.08338806368986695 1105 7 0.06980247321838107 1105 8 0.0902846916350839 1105 9 0.09152467417867172 1105 10-11 0.11646584342202004 1105 12-13 0.1595268989501406 1105 14-15 0.19358768033215057 1105 16-17 0.21604659422048655 1105 18-19 0.2065045941581829 1105 20-21 0.17605612560473816 1105 22-23 0.09659363564226453 1105 24-25 0.12862757077105158 1105 26-27 0.10805370604595765 1105 28-29 0.1264027868540012 1105 30-31 0.07854146498705461 1105 32-33 0.08477537082997344 1105 34-35 0.09672047080378121 1105 36-37 0.0781814382527557 1105 38-39 0.10474708445112668 1105 40-41 0.1572719426023852 1105 42-43 0.06492661944351852 1105 44-45 0.05859118871734381 1105 46-47 0.05155143920617178 1105 48-49 0.03789396082982677 1105 50-51 -0.019285588429980294 1105 52-53 0.0015520624982983122 1105 54-55 0.051203679061622154 1105 56-57 0.0324343755739136 1105 58-59 0.05155955766338138 1105 60-61 -0.008716962907577397 1105 62-63 0.0018964660103080178 1105 64-65 0.059390164422971736 1105 66-67 0.07129253802310842 1105 68-69 0.021183008093323963 1105 70-71 -0.055154062178296215 1105 72-73 -0.008474656701700667 1105 74-75 0.03938582692660475 1105 76-77 0.03646576973976323 1105 78-79 -0.02013632008548427 1105 80-81 -0.03243192087410307 1105 82-83 -0.04600448259500922 1105 84-85 -0.035920944477368266 1105 86-87 -0.05284959399464384 1105 88-89 -0.05769754261027771 1105 90-91 -0.06378920605357052 1105 92-93 -0.10152074316562931 1105 94-95 -0.10180845767226643 1105 96-97 -0.06262879313994318 1105 98-99 -0.022151867267446335 1105 100 -0.06491254056390261 1106 1 0.04804230108420171 1106 2 0.12604940202420778 1106 3 0.20474853310495433 1106 4 0.12784463915081545 1106 5 0.09323860110000481 1106 6 0.14407246913004457 1106 7 0.1260263706062119 1106 8 0.12570528165610284 1106 9 0.17086050353105975 1106 10-11 0.15945454699260608 1106 12-13 0.14852871524282563 1106 14-15 0.227711951608093 1106 16-17 0.16358789448220534 1106 18-19 0.19460846901029072 1106 20-21 0.21527102234475848 1106 22-23 0.1906673276580193 1106 24-25 0.14794124531621122 1106 26-27 0.1469888365747778 1106 28-29 0.16637041427438248 1106 30-31 0.20949197261988672 1106 32-33 0.2155513398647173 1106 34-35 0.18188050762893937 1106 36-37 0.16462402428086165 1106 38-39 0.1265378610198411 1106 40-41 0.12937271403134076 1106 42-43 0.09168929456442498 1106 44-45 0.12185904337377451 1106 46-47 0.15973157673172977 1106 48-49 0.12436613207042768 1106 50-51 0.14340918303036432 1106 52-53 0.05483638140912461 1106 54-55 0.17917855003329208 1106 56-57 0.15609124681994047 1106 58-59 0.11511487403213039 1106 60-61 0.05883064560606499 1106 62-63 0.09320717714410165 1106 64-65 0.10204971159291887 1106 66-67 0.09617693177847997 1106 68-69 0.06473174596679598 1106 70-71 0.11436724934099374 1106 72-73 0.1804060390951392 1106 74-75 0.13778110904393515 1106 76-77 0.1507264855963797 1106 78-79 0.05110869360298409 1106 80-81 -0.00485076872632817 1106 82-83 0.05554755070308204 1106 84-85 0.09045465208482995 1106 86-87 0.09855506659643964 1106 88-89 0.03863301596728519 1106 90-91 0.02606724840000396 1106 92-93 0.06535000870541907 1106 94-95 0.022474580285088308 1106 96-97 0.04904430403158244 1106 98-99 0.04823991095181057 1106 100 0.009777033473383767 1107 1 5.60005219732318E-4 1107 2 0.0429049479155168 1107 3 0.14214967937186174 1107 4 0.07016557382082311 1107 5 0.09419092257640926 1107 6 0.060495155303115666 1107 7 0.03976336525207813 1107 8 0.0919454339031418 1107 9 0.053718691875573654 1107 10-11 0.08647696671329186 1107 12-13 0.12084801468173367 1107 14-15 0.11945802228819247 1107 16-17 0.07996988797940219 1107 18-19 0.08439429386399411 1107 20-21 0.08251223949842768 1107 22-23 0.07652270400696182 1107 24-25 0.06941881456021548 1107 26-27 0.10104621613198361 1107 28-29 0.12929113516049995 1107 30-31 0.11190358737548678 1107 32-33 0.08413669441392813 1107 34-35 0.15230987367090876 1107 36-37 0.131312824251971 1107 38-39 0.15338369082940773 1107 40-41 0.173622293435848 1107 42-43 0.1326145394227325 1107 44-45 0.1501820988281679 1107 46-47 0.1555680325539015 1107 48-49 0.13158599089881307 1107 50-51 0.09451268814322589 1107 52-53 0.09646858783749934 1107 54-55 0.1855461575096058 1107 56-57 0.1304801042749375 1107 58-59 0.09680009756219476 1107 60-61 0.1333138047321114 1107 62-63 0.14418797722355237 1107 64-65 0.1076823679606349 1107 66-67 0.15841490605820496 1107 68-69 0.08462039475426764 1107 70-71 0.08499214086898377 1107 72-73 0.07786815791323676 1107 74-75 0.08782028436578315 1107 76-77 0.0679140812147665 1107 78-79 0.03817584642486338 1107 80-81 0.025410060666906986 1107 82-83 -0.0018702287734555512 1107 84-85 0.04813729134782818 1107 86-87 -0.02179635008553049 1107 88-89 -0.008760721599180954 1107 90-91 0.06769141661412448 1107 92-93 0.03328332739189577 1107 94-95 -0.048817081741734825 1107 96-97 -0.06695105261432488 1107 98-99 -0.007950241345387354 1107 100 0.0425105479863106 1108 1 -0.006064533046899356 1108 2 0.08228124485889765 1108 3 0.12826636768198085 1108 4 0.04445175105598764 1108 5 0.08209593485407396 1108 6 0.09476342288029116 1108 7 0.07443416961333327 1108 8 0.06860730222175704 1108 9 0.10535093254755878 1108 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2214 88-89 -0.47393006855743636 2214 90-91 -0.2943658124925186 2214 92-93 -0.22891097838385832 2214 94-95 -0.21146856605561837 2214 96-97 -0.24440829275221887 2214 98-99 -0.21343667590778637 2214 100 -0.18176725271120375 2215 1 -0.2771080352317341 2215 2 -0.3330687907051093 2215 3 -0.2666640490877157 2215 4 -0.2172766596682365 2215 5 -0.2528857474851307 2215 6 -0.19796620459896985 2215 7 -0.225905732406126 2215 8 -0.2497008809160448 2215 9 -0.3878894600711149 2215 10-11 -0.3931061491761767 2215 12-13 -0.43336255206261143 2215 14-15 -0.5927841066930526 2215 16-17 -0.5585275855727332 2215 18-19 -0.539117472025012 2215 20-21 -0.5376480622535738 2215 22-23 -0.5256207590829476 2215 24-25 -0.5007084536923472 2215 26-27 -0.4973958020750189 2215 28-29 -0.4176901529876389 2215 30-31 -0.5215856092720372 2215 32-33 -0.4947578642056243 2215 34-35 -0.5506380882076627 2215 36-37 -0.5886596868566158 2215 38-39 -0.6698725160489047 2215 40-41 -0.6642603333691639 2215 42-43 -0.578343730221583 2215 44-45 -0.5716265635964106 2215 46-47 -0.6244930836313642 2215 48-49 -0.5633704245908433 2215 50-51 -0.5859838435920182 2215 52-53 -0.608301417475495 2215 54-55 -0.582398499334488 2215 56-57 -0.5688223388900866 2215 58-59 -0.6248085066789599 2215 60-61 -0.5849214997403962 2215 62-63 -0.6250735977247572 2215 64-65 -0.7268421408408301 2215 66-67 -0.5762475525572697 2215 68-69 -0.527286396198761 2215 70-71 -0.5082994266775245 2215 72-73 -0.47936311684294 2215 74-75 -0.5124172670921823 2215 76-77 -0.5890751191387125 2215 78-79 -0.5298236795503115 2215 80-81 -0.5863076425598663 2215 82-83 -0.58824137504768 2215 84-85 -0.5081793294380432 2215 86-87 -0.6016441171995268 2215 88-89 -0.565228001926414 2215 90-91 -0.6614476800232687 2215 92-93 -0.5984170740973553 2215 94-95 -0.5713671196603549 2215 96-97 -0.4807510328380218 2215 98-99 -0.5455551997730375 2215 100 -0.5205386276248944 2216 1 -0.15510323172477314 2216 2 -0.18116440411629497 2216 3 -0.17747493104284118 2216 4 -0.19345504614321385 2216 5 -0.19199831401794398 2216 6 -0.2143606890612446 2216 7 -0.2566066235898248 2216 8 -0.2665063180305651 2216 9 -0.3417996552419993 2216 10-11 -0.3502014752521134 2216 12-13 -0.3971705321197305 2216 14-15 -0.5523487296898892 2216 16-17 -0.5720294621402786 2216 18-19 -0.5397833513828374 2216 20-21 -0.46402938672720495 2216 22-23 -0.47359575493359785 2216 24-25 -0.4789113742560289 2216 26-27 -0.5052732748717546 2216 28-29 -0.5490819393018285 2216 30-31 -0.5588665631498628 2216 32-33 -0.5327913766235213 2216 34-35 -0.5364122926129298 2216 36-37 -0.5861294529834993 2216 38-39 -0.43152363567353547 2216 40-41 -0.5080562251758991 2216 42-43 -0.6116516888268464 2216 44-45 -0.6458841284532468 2216 46-47 -0.5138268607019398 2216 48-49 -0.516257049895593 2216 50-51 -0.4702998429691405 2216 52-53 -0.44398266952560306 2216 54-55 -0.4691953163915059 2216 56-57 -0.4941835292135437 2216 58-59 -0.4360353068360112 2216 60-61 -0.4392209564015772 2216 62-63 -0.4686123150813941 2216 64-65 -0.4830975711095533 2216 66-67 -0.4826855458325028 2216 68-69 -0.4602656200312154 2216 70-71 -0.5047749974550655 2216 72-73 -0.48279702947900915 2216 74-75 -0.4195602744102658 2216 76-77 -0.35778538726301434 2216 78-79 -0.3446585797239621 2216 80-81 -0.3707295252330738 2216 82-83 -0.3961822632982006 2216 84-85 -0.49080117898479614 2216 86-87 -0.4355771709920724 2216 88-89 -0.44222687497739344 2216 90-91 -0.37914654857875973 2216 92-93 -0.4380560230827868 2216 94-95 -0.4848661160381518 2216 96-97 -0.4105771326218104 2216 98-99 -0.48604899249407296 2216 100 -0.5035470464964433 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 297.0 3 25.0 4 45.0 5 122.0 6 438.0 7 1072.0 8 2299.0 9 4025.0 10 4736.0 11 6134.0 12 9122.0 13 13205.0 14 14224.0 15 17304.0 16 21640.0 17 30100.0 18 30484.0 19 16408.0 20 11641.0 21 10790.0 22 11068.0 23 11549.0 24 13613.0 25 16398.0 26 20748.0 27 26858.0 28 34574.0 29 45105.0 30 58235.0 31 76371.0 32 99052.0 33 130663.0 34 181476.0 35 272232.0 36 435632.0 37 847587.0 38 1243155.0 39 510847.0 40 1595.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.660714712555595 20.44932646469877 14.108398489039239 22.781560333706395 2 16.11052008464455 21.24494991454474 39.08107294269806 23.563457058112647 3 17.53308835608004 26.009881185165508 31.553186827576084 24.903843631178372 4 12.73974684633346 18.182269410846803 38.305109423146874 30.77287431967286 5 12.063402686818158 37.03396663685831 35.4346689889293 15.467961687394233 6 28.780288456652205 38.525751614151346 18.223713801989714 14.470246127206732 7 24.995196022377435 33.2295091150305 23.402189951993314 18.373104910598745 8 24.21327627964846 35.050647987446546 21.612888510610944 19.12318722229405 9 24.123767481337758 16.745070575335706 22.866720761148596 36.26444118217794 10-11 22.907208897273822 26.92730500518924 29.461784328467743 20.70370176906919 12-13 24.197215749294056 24.777238434940905 29.65245437757586 21.373091438189178 14-15 21.539652964910992 26.75162005725065 27.882274303458697 23.82645267437966 16-17 20.042360091981102 29.78970750453394 28.34045440782969 21.82747799565527 18-19 19.75168694658237 27.640763635083005 31.911541104203415 20.696008314131213 20-21 21.04241469069357 27.14085451475808 31.669924074699544 20.146806719848804 22-23 20.689591192731328 27.18525437681951 31.717928397215793 20.40722603323336 24-25 20.50017384135505 27.065101755691323 31.813688866282554 20.621035536671073 26-27 20.30406755680689 26.91585345705575 31.955326435302062 20.824752550835303 28-29 20.43367450043951 26.95934334057613 31.922756294274297 20.68422586471006 30-31 20.8236298500379 27.44755273680182 31.393136965479197 20.335680447681078 32-33 20.222713111656258 27.169997463877987 31.95726457141547 20.650024853050283 34-35 20.377964905082145 26.760708024758035 31.87217566887559 20.98915140128423 36-37 21.05780160056953 27.3100868875874 31.088719598739644 20.54339191310343 38-39 20.859768997811088 27.34286975087151 31.235828856908594 20.561532394408808 40-41 20.531834003841766 27.202083070877404 31.363296760074583 20.90278616520625 42-43 20.49556485913414 27.283283883287336 31.10920002486487 21.111951232713658 44-45 20.889254666121783 27.524794551663028 30.72819082793629 20.857759954278894 46-47 20.95188955271364 27.943325591031066 30.12176694669582 20.98301790955948 48-49 20.935994473003063 27.669682044043437 30.175219322555247 21.219104160398253 50-51 21.005377382282457 27.588540321149154 29.963962486193736 21.442119810374656 52-53 21.039141131526407 27.777981308331697 29.726363543754246 21.45651401638765 54-55 21.199143722010774 28.290736489359514 29.512660401444712 20.997459387184996 56-57 20.82075809957718 27.949317267918243 29.608243601964514 21.62168103054006 58-59 20.68214591375909 27.51415843884554 30.076232565933854 21.727463081461515 60-61 21.182941376818803 28.022659174746373 29.527716410033022 21.266683038401805 62-63 21.02734686420213 27.910932718550256 29.67593655109624 21.385783866151375 64-65 20.986007839051503 27.605215382466348 29.957616272212633 21.451160506269513 66-67 20.882081198921547 27.743650299737478 29.686218126819806 21.68805037452117 68-69 21.40898240999662 27.937475728981447 29.45250727451027 21.20103458651166 70-71 21.47707716783479 27.5996255142856 29.580495165414007 21.342802152465605 72-73 21.12961899789381 27.856960354953088 29.80587439601652 21.207546251136584 74-75 21.11988104571425 27.851205036128512 29.59523209061779 21.43368182753945 76-77 21.278571848951124 27.97327215756385 29.216078304480707 21.532077689004318 78-79 21.410601462725506 28.124056783606395 29.323644858774877 21.141696894893226 80-81 21.176890889428023 27.808793934187605 29.560128385260725 21.454186791123647 82-83 21.04842065405541 27.92603471241414 29.60286108026296 21.422683553267493 84-85 21.169292651583355 27.8937340590807 29.4157188484508 21.521254440885144 86-87 21.32997167590102 28.07198648876544 29.228196370492498 21.369845464841045 88-89 21.006586868524042 27.94992107953925 29.36159043518997 21.68190161674674 90-91 20.887953587472747 27.810596972398272 29.419059877109525 21.88238956301945 92-93 21.114662662436004 28.12436293957834 29.1650028002591 21.59597159772656 94-95 20.95422488771781 27.851691075427254 29.397831361865606 21.79625267498933 96-97 20.90147970475867 27.945511581140785 29.385673402280965 21.76733531181958 98-99 20.878120242132646 27.960137292485634 29.357430938648676 21.80431152673305 100 21.07878648928765 27.99044639807699 29.11978992475534 21.81097718788002 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2000.0 1 1552.5 2 3638.5 3 5437.5 4 2916.5 5 1254.5 6 2507.0 7 5899.0 8 9350.5 9 10401.5 10 7680.0 11 4280.5 12 3549.0 13 3845.0 14 4285.5 15 4765.5 16 5291.5 17 5927.5 18 6701.5 19 7415.5 20 8475.5 21 10131.0 22 12593.5 23 16520.0 24 21532.0 25 27719.0 26 36401.0 27 46535.0 28 58313.5 29 71215.5 30 82487.0 31 92855.0 32 102946.5 33 111692.0 34 119519.5 35 126417.0 36 132465.0 37 138351.5 38 143717.0 39 146360.5 40 147432.0 41 146990.5 42 144086.5 43 141543.0 44 138956.5 45 137006.5 46 135407.0 47 132223.0 48 129132.0 49 127676.5 50 125043.5 51 119647.5 52 111770.5 53 104953.5 54 99941.0 55 94673.0 56 88031.5 57 80131.5 58 73218.5 59 66876.5 60 58904.0 61 49148.5 62 40030.0 63 32119.5 64 25673.0 65 20390.0 66 15612.5 67 11585.0 68 8464.0 69 6117.5 70 4344.0 71 3062.5 72 2222.5 73 1598.5 74 1106.5 75 708.0 76 441.5 77 264.5 78 161.5 79 96.0 80 57.0 81 26.5 82 16.5 83 13.5 84 6.5 85 2.5 86 1.5 87 1.0 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 1.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12947694669818421 2 0.0 3 0.0 4 0.0 5 0.012101532805671836 6 1.8908645008862244E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.2454015948023914E-4 82-83 4.6089822209101724E-4 84-85 2.836296751329337E-4 86-87 4.6089822209101724E-4 88-89 6.381667690491008E-4 90-91 6.736204784407175E-4 92-93 2.48175965741317E-4 94-95 2.599938688718559E-4 96-97 5.908951565269452E-4 98-99 0.0011227007974011958 100 9.454322504431122E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4230869.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.53284787141925 #Duplication Level Percentage of deduplicated Percentage of total 1 78.58184644309057 50.71110341967192 2 13.674729418945358 17.649384665512446 3 3.6405780702511525 7.048106123146282 4 1.4369880542027893 3.709317259796615 5 0.7479195592293457 2.4132689567903163 6 0.45743364215414356 1.7711697384241547 7 0.2943580175840734 1.3297032817939896 8 0.20140436979984097 1.0397758045545764 9 0.1519607103390818 0.8825811662470295 >10 0.7658911611088153 8.499138801455716 >50 0.03227092359513981 1.4208816950296788 >100 0.013668258771678524 1.514822849244629 >500 4.390943415165623E-4 0.2024607652936212 >1k 3.6591184752496044E-4 0.535337885833979 >5k 3.659118475246042E-5 0.1666629084828447 >10k+ 1.0977355425738126E-4 1.106284678722198 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18260 0.43158982232728077 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17666 0.4175501534082005 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10924 0.258197547596014 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7058 0.16682152059068717 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4783 0.11305006134673516 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4590 0.10848835073834714 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 2.3635806261077806E-5 0.0 9 0.0 0.0 0.0 7.090741878323342E-5 0.0 10-11 0.0 0.0 0.0 9.454322504431122E-5 0.0 12-13 0.0 0.0 0.0 9.454322504431122E-5 0.0 14-15 0.0 1.1817903130538903E-5 0.0 1.4181483756646685E-4 0.0 16-17 0.0 2.3635806261077806E-5 0.0 1.8908645008862244E-4 0.0 18-19 0.0 2.3635806261077806E-5 0.0 1.8908645008862244E-4 0.0 20-21 0.0 2.3635806261077806E-5 0.0 2.1272225634970024E-4 0.0 22-23 0.0 2.3635806261077806E-5 0.0 3.427191907856282E-4 0.0 24-25 0.0 2.3635806261077806E-5 0.0 4.2544451269940054E-4 0.0 26-27 0.0 2.3635806261077806E-5 0.0 8.036174128766454E-4 0.0 28-29 0.0 3.545370939161671E-5 0.0 0.0016072348257532908 0.0 30-31 0.0 7.090741878323342E-5 0.0 0.0036044604548143657 0.0 32-33 0.0 7.090741878323342E-5 0.0 0.007906177194330526 0.0 34-35 0.0 7.090741878323342E-5 0.0 0.015386909875961651 0.0 36-37 0.0 1.1817903130538903E-4 0.0 0.02544394544005026 0.0 38-39 0.0 1.6545064382754465E-4 0.0 0.038290006142946045 0.0 40-41 0.0 1.8908645008862244E-4 0.0 0.05295602392794482 0.0 42-43 0.0 2.599938688718559E-4 0.0 0.06861474557590888 0.0 44-45 0.0 2.599938688718559E-4 0.0 0.08716885349085496 0.0 46-47 0.0 2.718117720023948E-4 0.0 0.10861834767278306 0.0 48-49 0.0 2.836296751329337E-4 0.0 0.13071782652689082 0.0 50-51 0.0 2.836296751329337E-4 0.0 0.1535854690844836 0.0 52-53 0.0 2.836296751329337E-4 0.0 0.17611039245129073 0.0 54-55 0.0 2.836296751329337E-4 0.0 0.20215705095099848 0.0 56-57 0.0 2.836296751329337E-4 0.0 0.23319086457179364 0.0 58-59 0.0 3.072654813940115E-4 0.0 0.2658910025339948 0.0 60-61 0.0 3.427191907856282E-4 0.0 0.30149834466630854 0.0 62-63 0.0 3.545370939161671E-4 0.0 0.33832293082106774 0.0 64-65 0.0 3.66354997046706E-4 0.0 0.37844471194924734 0.0 66-67 0.0 4.0180870643832274E-4 0.0 0.4261890405966245 0.0 68-69 0.0 4.0180870643832274E-4 0.0 0.47805781743655973 0.0 70-71 0.0 4.0180870643832274E-4 0.0 0.5355637340697621 0.0 72-73 0.0 4.2544451269940054E-4 0.0 0.598328617596054 0.0 74-75 0.0 4.2544451269940054E-4 0.0 0.6638352546486312 0.0 76-77 0.0 4.2544451269940054E-4 0.0 0.7367989885765784 0.0 78-79 0.0 4.2544451269940054E-4 0.0 0.8195124925872204 0.0 80-81 0.0 4.2544451269940054E-4 0.0 0.910994880720722 0.0 82-83 0.0 4.2544451269940054E-4 0.0 1.0088589365447145 0.0 84-85 0.0 4.4908031896047834E-4 0.0 1.1116746937804032 0.0 86-87 0.0 4.7271612522155614E-4 0.0 1.2236729617485202 0.0 88 0.0 4.7271612522155614E-4 0.0 1.3209106687065943 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9055 0.0 58.11353 1 TATAACG 405 0.0 39.461395 2 GTATCAA 15760 0.0 38.735367 1 GTGGTAT 3410 0.0 32.022667 1 ATCAACG 19810 0.0 30.34832 3 TCAACGC 20030 0.0 30.038458 4 TATCAAC 20410 0.0 29.594343 2 ATAACGC 480 0.0 29.378433 3 CAACGCA 20830 0.0 28.884794 5 TGGTATC 3845 0.0 28.11772 2 GTACCTG 3265 0.0 27.678461 1 AACGCAG 21895 0.0 27.647629 6 TACCTGG 3305 0.0 26.169472 2 ACGCAGA 24870 0.0 24.20808 7 CGCAGAG 25145 0.0 23.98071 8 TAACGCA 670 0.0 23.151957 4 GCAGAGT 27460 0.0 21.736528 9 GCGGTAT 335 1.8189894E-12 21.075132 1 CAGAGTA 26780 0.0 20.445717 10-11 GAGTACT 21725 0.0 20.248976 12-13 >>END_MODULE