FastQCFastQC Report
Wed 25 May 2016
SRR2049448_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049448_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5771508
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65400.043.1424641
GTATCAA107900.034.6043361
TCAACGC113650.032.0915224
ATCAACG114450.031.8683093
CAACGCA123250.029.78365
AACGCAG131650.028.025326
TATCAAC131550.027.8687082
ACGCAGA150300.024.3293157
CGCAGAG149750.024.165078
GTACATG178750.023.4402981
TACATGG179950.022.5670592
ACATGGG181250.021.3938543
GTGGTAT36850.019.9075831
GCAGAGT178250.019.246929
CATGGGG120650.018.6987234
AGAGTAC153000.017.43187910-11
CAGAGTA168200.017.28158210-11
GTACCTG45050.017.2234671
GAGTACA135550.016.652261
TACCTGG43800.016.4183832
Produced by FastQC (version 0.11.5)