##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049448_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5771508
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.0356546330699	31.0	31.0	34.0	28.0	34.0
2	31.09445191793895	31.0	31.0	34.0	28.0	34.0
3	31.258207040516968	33.0	31.0	34.0	28.0	34.0
4	34.827949298519556	37.0	35.0	37.0	32.0	37.0
5	34.865948899317125	37.0	35.0	37.0	32.0	37.0
6	34.90763575134956	37.0	35.0	37.0	32.0	37.0
7	34.76384594806071	37.0	35.0	37.0	32.0	37.0
8	34.7647820985434	37.0	35.0	37.0	32.0	37.0
9	36.33081925902208	39.0	37.0	39.0	32.0	39.0
10-11	36.285541491062645	39.0	37.0	39.0	32.0	39.0
12-13	36.22600627080479	39.0	37.0	39.0	32.0	39.0
14-15	37.46874101188112	40.0	38.0	41.0	32.0	41.0
16-17	37.456575213964875	40.0	38.0	41.0	32.0	41.0
18-19	37.4678769396144	40.0	38.0	41.0	32.0	41.0
20-21	37.518861708239854	40.0	38.0	41.0	32.0	41.0
22-23	37.475807016121266	40.0	38.0	41.0	32.0	41.0
24-25	37.47870911727057	40.0	38.0	41.0	32.0	41.0
26-27	37.477412835605534	40.0	38.0	41.0	32.0	41.0
28-29	37.45831877907819	40.0	38.0	41.0	32.0	41.0
30-31	37.40572429250726	40.0	38.0	41.0	32.0	41.0
32-33	37.367047832213004	40.0	38.0	41.0	32.0	41.0
34-35	37.30422898140313	40.0	38.0	41.0	31.0	41.0
36-37	37.19742517900001	40.0	37.0	41.0	31.0	41.0
38-39	37.10027951100475	40.0	37.0	41.0	31.0	41.0
40-41	36.98932211477486	40.0	37.0	41.0	30.0	41.0
42-43	36.897418837503125	40.0	37.0	41.0	30.0	41.0
44-45	36.74776982029653	40.0	37.0	41.0	30.0	41.0
46-47	36.6328793098788	40.0	36.5	41.0	30.0	41.0
48-49	36.53888325200277	40.0	36.0	41.0	30.0	41.0
50-51	36.428209317218304	40.0	36.0	41.0	30.0	41.0
52-53	36.32040101131281	40.0	36.0	41.0	30.0	41.0
54-55	36.178399302227426	39.0	35.0	41.0	29.5	41.0
56-57	36.02130690973659	39.0	35.0	41.0	29.0	41.0
58-59	35.845193925053906	39.0	35.0	41.0	29.0	41.0
60-61	35.65346015287513	39.0	35.0	41.0	28.5	41.0
62-63	35.40930723824692	38.5	35.0	40.0	28.0	41.0
64-65	35.174815750060475	38.0	35.0	40.0	28.0	41.0
66-67	34.94904312703023	37.5	34.0	40.0	28.0	41.0
68-69	34.66429926112898	37.0	34.0	40.0	28.0	41.0
70-71	34.354127465473496	36.5	34.0	39.5	27.5	41.0
72-73	34.02158439354152	36.0	34.0	39.0	26.5	41.0
74-75	33.72029355239567	36.0	34.0	39.0	26.0	40.5
76-77	33.39521300152404	35.0	34.0	38.5	26.0	40.0
78-79	33.04908769077336	35.0	33.0	37.0	26.0	39.0
80-81	32.71911474436144	35.0	33.0	37.0	26.0	39.0
82-83	32.424989967959846	35.0	33.0	36.5	26.0	39.0
84-85	32.14049534367794	35.0	33.0	36.0	25.5	37.0
86-87	31.875686302436037	35.0	33.0	36.0	25.0	37.0
88-89	31.65535965643641	35.0	33.0	35.0	25.0	37.0
90-91	31.45473002896297	35.0	33.0	35.0	24.5	36.0
92-93	31.259064615348365	35.0	32.5	35.0	24.0	36.0
94-95	31.06758242386565	35.0	32.0	35.0	23.0	36.0
96-97	30.88870439060294	35.0	32.0	35.0	21.0	36.0
98-99	30.723713975619546	35.0	32.0	35.0	19.5	35.0
100	30.59778224339289	35.0	32.0	35.0	18.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3821361575726172
1101	2	0.374228625690769
1101	3	0.4031613030328991
1101	4	0.40530013432559286
1101	5	0.38698311216319325
1101	6	0.38446738992293916
1101	7	0.5061699635804189
1101	8	0.5249738129243156
1101	9	0.6025327033925265
1101	10-11	0.6361562461399899
1101	12-13	0.6403659982905552
1101	14-15	0.7209021644057572
1101	16-17	0.672932133635797
1101	18-19	0.671115047116821
1101	20-21	0.6811460386080981
1101	22-23	0.6684549919153326
1101	24-25	0.7116741263137953
1101	26-27	0.7310320283400316
1101	28-29	0.6681472218953459
1101	30-31	0.7177736480422041
1101	32-33	0.7157914113658705
1101	34-35	0.6577134462625125
1101	36-37	0.6469107578230862
1101	38-39	0.7039326637312087
1101	40-41	0.7169484570478062
1101	42-43	0.6473595424994016
1101	44-45	0.6759827727331853
1101	46-47	0.643031263667126
1101	48-49	0.6194038905050689
1101	50-51	0.6262651306935609
1101	52-53	0.6198025465138599
1101	54-55	0.5825053210461277
1101	56-57	0.5436566933864597
1101	58-59	0.5652702841144404
1101	60-61	0.6000713921609773
1101	62-63	0.5807226564191552
1101	64-65	0.6174393165358509
1101	66-67	0.5680838270475661
1101	68-69	0.5264742100371294
1101	70-71	0.5208094339934206
1101	72-73	0.5230532647830302
1101	74-75	0.5064793517114836
1101	76-77	0.43971543578523153
1101	78-79	0.44779152894446383
1101	80-81	0.44780894524099324
1101	82-83	0.4528606266710824
1101	84-85	0.4478956495380686
1101	86-87	0.4882192726171617
1101	88-89	0.46880119490946015
1101	90-91	0.46480031375845954
1101	92-93	0.4689481637061341
1101	94-95	0.4132365507370146
1101	96-97	0.4177909619104909
1101	98-99	0.488311099290744
1101	100	0.44648856742557186
1102	1	0.2667115415764272
1102	2	0.2580899921500617
1102	3	0.29384124676409584
1102	4	0.32258326720242536
1102	5	0.3130036702201764
1102	6	0.2862620288603068
1102	7	0.3579591817599024
1102	8	0.4008078734868974
1102	9	0.3956522725962017
1102	10-11	0.430671702326876
1102	12-13	0.41242935289646
1102	14-15	0.4939418577380863
1102	16-17	0.4536758950796198
1102	18-19	0.46173221713321055
1102	20-21	0.473577514539933
1102	22-23	0.47534145414135054
1102	24-25	0.46040796107838133
1102	26-27	0.3741564912834576
1102	28-29	0.38882310507734275
1102	30-31	0.3828834334471125
1102	32-33	0.3914104652629291
1102	34-35	0.39338590633629167
1102	36-37	0.4403481440540915
1102	38-39	0.432998399455208
1102	40-41	0.4187716637138692
1102	42-43	0.37432667530663366
1102	44-45	0.3376768114387332
1102	46-47	0.29299054942538305
1102	48-49	0.30144419183228877
1102	50-51	0.322896698510597
1102	52-53	0.25777106562973273
1102	54-55	0.3023439982875118
1102	56-57	0.33133616397248744
1102	58-59	0.27423630131630716
1102	60-61	0.24276234504990413
1102	62-63	0.21546968939075128
1102	64-65	0.2522605084868914
1102	66-67	0.2722957308311251
1102	68-69	0.28178592536568914
1102	70-71	0.23985945788491847
1102	72-73	0.2586794560113148
1102	74-75	0.2909884652770245
1102	76-77	0.22161445442728933
1102	78-79	0.24360268011267294
1102	80-81	0.26698169743338696
1102	82-83	0.23997537641945144
1102	84-85	0.2926168351983591
1102	86-87	0.23290807594135643
1102	88-89	0.21375968930399836
1102	90-91	0.20083849095165718
1102	92-93	0.23853024167670966
1102	94-95	0.3016986265125148
1102	96-97	0.29778263502407043
1102	98-99	0.3203430519815811
1102	100	0.2807801981769309
1103	1	0.2793417626915726
1103	2	0.2576027494224036
1103	3	0.307341398100764
1103	4	0.2716433948978505
1103	5	0.30902060152151023
1103	6	0.2827368047184251
1103	7	0.2793638110259593
1103	8	0.28710392891701986
1103	9	0.3917888416711648
1103	10-11	0.4029948582690963
1103	12-13	0.33911491619608825
1103	14-15	0.39675406120002066
1103	16-17	0.3940630510954577
1103	18-19	0.3776273735416993
1103	20-21	0.351602586322592
1103	22-23	0.37599186949916685
1103	24-25	0.3777978227484837
1103	26-27	0.34484664274283006
1103	28-29	0.33089196428515066
1103	30-31	0.40370078951367105
1103	32-33	0.4380970613330817
1103	34-35	0.36342060640337337
1103	36-37	0.33931429985724293
1103	38-39	0.39395200454011814
1103	40-41	0.44051288925458465
1103	42-43	0.36151418553208003
1103	44-45	0.3130379487951629
1103	46-47	0.30241343009846844
1103	48-49	0.28936039324094054
1103	50-51	0.24259418877596062
1103	52-53	0.2999432530172399
1103	54-55	0.18177992663572695
1103	56-57	0.2025494239279837
1103	58-59	0.18166861905787357
1103	60-61	0.2100681279513168
1103	62-63	0.2148982145126439
1103	64-65	0.2575641941483582
1103	66-67	0.22893381225024712
1103	68-69	0.23104225389715793
1103	70-71	0.302154565415762
1103	72-73	0.3231933585341409
1103	74-75	0.2485523334423405
1103	76-77	0.15715423552083507
1103	78-79	0.22779144909110727
1103	80-81	0.2691331322686352
1103	82-83	0.27047572306222634
1103	84-85	0.27312099082153907
1103	86-87	0.24258096888498315
1103	88-89	0.25011525685741276
1103	90-91	0.24063914067679804
1103	92-93	0.21963654853021453
1103	94-95	0.25495131682860617
1103	96-97	0.2090470682477843
1103	98-99	0.2139839208585812
1103	100	0.21336968109633858
1104	1	0.1428001843324722
1104	2	0.08170335248412286
1104	3	0.22750355722622118
1104	4	0.23431160153094055
1104	5	0.21856253678270576
1104	6	0.19310380067500432
1104	7	0.21529731561354737
1104	8	0.2139116453458243
1104	9	0.284405925384533
1104	10-11	0.3188956725346017
1104	12-13	0.3532115848895998
1104	14-15	0.40978020721556874
1104	16-17	0.3678948668605386
1104	18-19	0.3278934620416649
1104	20-21	0.3496436179458797
1104	22-23	0.34054349656144467
1104	24-25	0.34232516982034866
1104	26-27	0.292931066243149
1104	28-29	0.30111184461279805
1104	30-31	0.309587461717463
1104	32-33	0.33846391408820864
1104	34-35	0.3713097673750312
1104	36-37	0.35200428963485564
1104	38-39	0.3547212313550645
1104	40-41	0.3687472960847842
1104	42-43	0.4086704249296673
1104	44-45	0.32863171527251467
1104	46-47	0.29679690376921997
1104	48-49	0.274584527386196
1104	50-51	0.27400675914663
1104	52-53	0.24753073992716423
1104	54-55	0.22689389296355955
1104	56-57	0.23197915901263855
1104	58-59	0.27205898439049037
1104	60-61	0.27287110000573733
1104	62-63	0.3581179376858685
1104	64-65	0.33880366516952165
1104	66-67	0.3371961323488293
1104	68-69	0.30969377168570844
1104	70-71	0.29319024607798383
1104	72-73	0.2631302158717119
1104	74-75	0.27149995365444823
1104	76-77	0.2589682675010252
1104	78-79	0.29555864648439467
1104	80-81	0.3574193082610222
1104	82-83	0.3293739310612409
1104	84-85	0.25799400601264466
1104	86-87	0.2584571910543225
1104	88-89	0.2611163874145461
1104	90-91	0.30843819121442806
1104	92-93	0.26002550938701674
1104	94-95	0.28557903306647603
1104	96-97	0.3598968587330411
1104	98-99	0.3647915770693402
1104	100	0.3423465507565737
1105	1	0.12399114017743784
1105	2	0.1522603006821015
1105	3	0.15228468362441916
1105	4	0.1391263565073828
1105	5	0.20444191788409682
1105	6	0.2542280099056953
1105	7	0.15738549869256957
1105	8	0.22976688446248517
1105	9	0.23659211458693363
1105	10-11	0.2710677348689785
1105	12-13	0.27041279544059904
1105	14-15	0.345665523517539
1105	16-17	0.25481567006134753
1105	18-19	0.2752935317312719
1105	20-21	0.3063400363714166
1105	22-23	0.2524172050436775
1105	24-25	0.21913872584794802
1105	26-27	0.2061104598988166
1105	28-29	0.2976159643081786
1105	30-31	0.29029118431594014
1105	32-33	0.30131057541775874
1105	34-35	0.3059787286518443
1105	36-37	0.3235140667097838
1105	38-39	0.3103667714490328
1105	40-41	0.3961961028058454
1105	42-43	0.3381510452101537
1105	44-45	0.2951534183128004
1105	46-47	0.2646744176774476
1105	48-49	0.22231452613607416
1105	50-51	0.2205068213284349
1105	52-53	0.19058806152545316
1105	54-55	0.13990105968510136
1105	56-57	0.13303294672735433
1105	58-59	0.09221764628883733
1105	60-61	0.18483348277226241
1105	62-63	0.19925519757811117
1105	64-65	0.17704121774806936
1105	66-67	0.15189731320171518
1105	68-69	0.162335755405131
1105	70-71	0.21494158301617006
1105	72-73	0.17262117787442577
1105	74-75	0.1922290123522714
1105	76-77	0.12977671079622155
1105	78-79	0.16103524721772544
1105	80-81	0.15789726066368814
1105	82-83	0.10092049989481211
1105	84-85	0.0948494468406409
1105	86-87	0.11259440463815551
1105	88-89	0.10273276699997069
1105	90-91	0.08186041313009085
1105	92-93	0.07423364264867516
1105	94-95	0.08820689286557126
1105	96-97	0.069516566098617
1105	98-99	0.13069292563475443
1105	100	0.11476252993777081
1106	1	0.14172516127673873
1106	2	0.1677692717423156
1106	3	0.1516847061497515
1106	4	0.14456845467752544
1106	5	0.18989538130397676
1106	6	0.15532472017382304
1106	7	0.14450057957540707
1106	8	0.17141246063840043
1106	9	0.17108617468741016
1106	10-11	0.1913881517059295
1106	12-13	0.16523291582220878
1106	14-15	0.2675388919534498
1106	16-17	0.23827215067933594
1106	18-19	0.2587101620251957
1106	20-21	0.23855922284208475
1106	22-23	0.27129114202701743
1106	24-25	0.29400982671920417
1106	26-27	0.2492546761129617
1106	28-29	0.2515013530949659
1106	30-31	0.259540732331061
1106	32-33	0.27674362712267
1106	34-35	0.3012899255599635
1106	36-37	0.3237250796609956
1106	38-39	0.3508131192993389
1106	40-41	0.36109052473644
1106	42-43	0.29251554972722715
1106	44-45	0.23407134880013558
1106	46-47	0.25140056253187737
1106	48-49	0.2170034695660661
1106	50-51	0.2244399001392523
1106	52-53	0.24636584204192502
1106	54-55	0.1723622474712414
1106	56-57	0.19585711796492689
1106	58-59	0.1991806533769065
1106	60-61	0.19953917844073032
1106	62-63	0.25218669327532695
1106	64-65	0.2069282697907866
1106	66-67	0.24982075797941405
1106	68-69	0.25436850447838566
1106	70-71	0.2181212242735029
1106	72-73	0.17289930378649387
1106	74-75	0.1598476961845492
1106	76-77	0.17417981684189243
1106	78-79	0.14523635329573636
1106	80-81	0.24423773929643033
1106	82-83	0.1995310386314486
1106	84-85	0.20153672160064673
1106	86-87	0.17394830978113518
1106	88-89	0.2012008376223804
1106	90-91	0.1559108423830473
1106	92-93	0.12792322059213745
1106	94-95	0.17687333674845718
1106	96-97	0.20855887746870394
1106	98-99	0.2299476661599158
1106	100	0.2494983277745284
1107	1	0.08308526241932768
1107	2	0.10996147068055251
1107	3	0.12309772163950683
1107	4	0.08798822009202922
1107	5	0.08399654031803294
1107	6	0.14614729599573906
1107	7	0.14137625139147758
1107	8	0.08602852122733395
1107	9	0.123367511087487
1107	10-11	0.1587422266130858
1107	12-13	0.16366514772743557
1107	14-15	0.18498825906345928
1107	16-17	0.15191891202466934
1107	18-19	0.1752267042202007
1107	20-21	0.1567352262122128
1107	22-23	0.1424796109216615
1107	24-25	0.12957814265356404
1107	26-27	0.19726392658241565
1107	28-29	0.18071637240220895
1107	30-31	0.15575368250519261
1107	32-33	0.15271419451345736
1107	34-35	0.1308992057886016
1107	36-37	0.11491597373616713
1107	38-39	0.09700860527508581
1107	40-41	0.17240519963905854
1107	42-43	0.13565347244899328
1107	44-45	0.13290600607913206
1107	46-47	0.07994546871072572
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2216	100	-0.918983446969758
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	88167.0
3	16957.0
4	10229.0
5	8459.0
6	7198.0
7	7108.0
8	7545.0
9	8547.0
10	9697.0
11	10892.0
12	11975.0
13	13209.0
14	14826.0
15	15854.0
16	15805.0
17	17256.0
18	16812.0
19	15272.0
20	15324.0
21	16650.0
22	19094.0
23	23208.0
24	27940.0
25	34701.0
26	42397.0
27	48961.0
28	58626.0
29	73434.0
30	90185.0
31	105537.0
32	134211.0
33	180385.0
34	252973.0
35	371152.0
36	623755.0
37	1139863.0
38	1599110.0
39	616825.0
40	1369.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.16762239011204	20.550146436368237	14.521216366886827	22.7610148066329
2	15.256341408004943	20.733019440590244	40.50292500417189	23.50771414723292
3	18.001239410337362	26.010824738158973	30.723356750194004	25.264579101309664
4	12.478911361872019	17.576296855938782	39.35943911500499	30.585352667184207
5	12.874251081963031	38.13543668055683	35.51878082658926	13.471531410890883
6	29.733444877366832	38.487058131237745	18.425402790518433	13.35409420087699
7	26.31175344045382	32.8772749557352	23.139851798501557	17.671119805309417
8	24.54777052845937	36.283626097823515	21.05876938410983	18.10983398960728
9	24.61527424911549	17.45787640742652	22.073008566635142	35.85384077682285
10-11	23.280622835751295	27.481537549514652	29.457366759046067	19.780472855687986
12-13	23.80456523012438	25.143449746320268	29.315787339476824	21.736197684078533
14-15	21.377881072698013	27.4364284987123	26.47037647532799	24.7153139532617
16-17	20.932082287731536	30.574879615900706	27.06512329877342	21.427914797594337
18-19	20.867013861513094	29.587602874017826	28.99872032909289	20.546662935376187
20-21	22.031873334975526	28.786681658367197	28.502810337341856	20.678634669315425
22-23	21.71644912140735	28.671690876372853	28.52476129141037	21.08709871080943
24-25	21.448480782432593	28.768528018270484	28.493028953482195	21.289962245814724
26-27	21.259780031806223	28.799895619592323	28.475127694457893	21.465196654143558
28-29	21.316154696454277	28.72831045757693	28.700368111171898	21.255166734796894
30-31	21.60237354273381	29.088435416585984	28.210304661198414	21.09888637948179
32-33	21.226740698683805	28.858216655419017	28.61315686209443	21.301885783802746
34-35	21.483621232200683	28.63238103291487	28.55593858126691	21.32805915361753
36-37	21.718405479329096	28.794818092869157	28.19683775158768	21.289938676214064
38-39	21.466636050914573	28.983710109263576	28.33059813359443	21.219055706227415
40-41	21.437233902172608	28.66572000194428	28.497164121389694	21.399881974493425
42-43	21.342336095716487	28.742704850517935	28.413821521185902	21.50113753257968
44-45	21.488627851268923	28.852491119468976	28.329255733731806	21.329625295530295
46-47	21.55496535140407	28.91954226560296	28.14084999102332	21.38464239196965
48-49	21.424393167089896	28.748983950908357	28.284371919414102	21.542250962587644
50-51	21.43669466913421	28.71435576996389	28.236658972919397	21.612290587982503
52-53	21.466762919389442	28.730675304921107	28.20132287422506	21.601238901464395
54-55	21.60175107182796	28.974986507656435	28.123381146207617	21.29988127430799
56-57	21.512345384832006	28.769880640339668	28.090566037735847	21.62720793709248
58-59	21.425565215673682	28.49352693921957	28.371233852001797	21.709673993104943
60-61	21.644437717456878	28.82819621267327	28.04620701145212	21.481159058417738
62-63	21.480446678087116	28.87504660442115	28.131403369485568	21.51310334800616
64-65	21.58061972721438	28.641667080435113	28.171686782745397	21.60602640960511
66-67	21.41711715520904	28.695640721899945	28.14190198469113	21.745340138199882
68-69	21.709595171261302	28.853070585290396	28.042614244598944	21.39471999884936
70-71	21.704375760335033	28.562523012063195	28.138134856734442	21.594966370867326
72-73	21.47802090395245	28.709358047078954	28.28635385602919	21.526267192939404
74-75	21.52344552864521	28.74827956828992	28.145890182820672	21.5823847202442
76-77	21.62796556981117	28.795168655871738	27.937030474189662	21.63983530012743
78-79	21.6518872864753	28.78961398941374	28.08862013717843	21.469878586932527
80-81	21.571613109394917	28.68055329304956	28.173335070937938	21.57449852661758
82-83	21.48222237182508	28.72966639400246	28.29435673471488	21.493754499457573
84-85	21.4815623474287	28.659581943269362	28.174923440886484	21.68393226841545
86-87	21.639365199514987	28.787811046016433	28.066703647421942	21.506120107046637
88-89	21.505770998663067	28.691493961267895	28.129704288847122	21.673030751221916
90-91	21.395491419410774	28.666486097471665	28.177666917765286	21.76035556535227
92-93	21.53007100681841	28.83495257504875	28.006379411219644	21.628597006913193
94-95	21.450172182180392	28.640095227004597	28.21845163580003	21.69128095501498
96-97	21.507792672853995	28.73057658561716	28.157568478471735	21.60406226305711
98-99	21.414515194848686	28.76614389045829	28.079264211583787	21.740076703109235
100	21.496463766428377	28.758305158520574	28.019383439448063	21.72584763560298
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3304.0
1	2422.0
2	1807.5
3	2084.5
4	1874.5
5	1556.0
6	1399.5
7	1358.0
8	1437.0
9	1488.0
10	1324.5
11	1130.0
12	1098.0
13	1144.5
14	1261.5
15	1430.0
16	1659.0
17	2024.5
18	2739.0
19	3668.5
20	4973.5
21	7256.0
22	10610.5
23	15899.0
24	23092.0
25	31766.5
26	43660.5
27	58807.0
28	77655.5
29	97317.5
30	114355.5
31	130584.5
32	146344.5
33	160401.5
34	172404.5
35	182306.0
36	190505.5
37	198770.5
38	205666.5
39	208154.5
40	210232.5
41	210898.0
42	207662.5
43	204142.5
44	199356.0
45	194312.5
46	189501.0
47	182609.0
48	177628.0
49	173903.0
50	168748.5
51	161251.0
52	149149.0
53	139683.5
54	134852.5
55	129820.0
56	120689.0
57	108257.0
58	97021.5
59	87597.0
60	77179.5
61	65277.0
62	54981.0
63	45511.5
64	36799.0
65	29651.5
66	23803.5
67	18709.5
68	14499.0
69	11369.0
70	8605.0
71	6318.5
72	4690.0
73	3435.0
74	2446.5
75	1749.5
76	1153.0
77	787.5
78	560.0
79	398.0
80	277.5
81	192.0
82	131.0
83	102.0
84	89.5
85	59.0
86	49.5
87	47.5
88	41.5
89	28.5
90	19.5
91	16.5
92	16.0
93	15.0
94	12.0
95	10.5
96	12.5
97	9.5
98	6.0
99	8.5
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07793457099946842
2	0.012942891181992643
3	0.01770767709236477
4	0.010881038369867979
5	0.00894047101728006
6	0.006826638722496789
7	0.01942300002009873
8	0.00942561285542704
9	0.00890581802884099
10-11	0.012838932216675434
12-13	0.014658214109726609
14-15	0.009451602596756342
16-17	0.012275821154540545
18-19	0.008541961650230754
20-21	0.013159472359736833
22-23	0.012414433108296827
24-25	0.015411916608276382
26-27	0.00537121320805585
28-29	0.012076566471015895
30-31	0.009642194033171226
32-33	0.011634740868417752
34-35	0.0086632471097675
36-37	0.010092682882879137
38-39	0.01833143088426803
40-41	0.012501065579394503
42-43	0.008048156564974006
44-45	0.020107396541770368
46-47	0.017326494219535
48-49	0.017231198501327556
50-51	0.010187978601086578
52-53	0.010205305095306113
54-55	0.011132272536051237
56-57	0.019630917950733153
58-59	0.0022004647658809447
60-61	0.006800648981167488
62-63	0.014978754252788006
64-65	0.023780613316311785
66-67	0.01275229974557776
68-69	0.014008470576494046
70-71	0.024889508946362028
72-73	0.019336367549001057
74-75	0.019994774329343386
76-77	0.00911373595947541
78-79	0.010915691358307049
80-81	0.01926706157212292
82-83	0.011669393856856822
84-85	0.011097619547612167
86-87	0.016269578072143364
88-89	0.014606234627068004
90-91	0.015022070488336842
92-93	0.007666973692144237
94-95	0.015178008936312659
96-97	0.012726310004248457
98-99	0.009624867538951691
100	0.006861291710935859
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5771508.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.655196051221914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.85037892055144	46.00719603985517
2	14.578335127309055	17.68503550494692
3	4.437522305745836	8.07476356310052
4	1.7821934824716155	4.32397180322103
5	0.9360933355774059	2.8389462395844913
6	0.5686240294192261	2.0694001190315365
7	0.37886327385076224	1.608601830641803
8	0.25426151172037426	1.233782547334349
9	0.1860197938945615	1.0154760361274269
>10	0.9540988815612569	10.181563832201999
>50	0.049406864731755484	2.045782657307466
>100	0.0232301552353125	2.445424746567926
>500	8.579292698938821E-4	0.34717837696929293
>1k	1.1438866159481932E-4	0.12287670310991466
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.7326494219535E-5	0.0
5	0.0	0.0	0.0	1.7326494219535E-5	0.0
6	0.0	0.0	0.0	1.7326494219535E-5	0.0
7	0.0	0.0	0.0	1.7326494219535E-5	0.0
8	0.0	0.0	0.0	1.7326494219535E-5	0.0
9	0.0	0.0	0.0	5.1979482658604996E-5	0.0
10-11	0.0	1.7326494219535E-5	0.0	5.1979482658604996E-5	0.0
12-13	0.0	3.465298843907E-5	0.0	6.930597687814E-5	0.0
14-15	0.0	3.465298843907E-5	0.0	7.79692239879075E-5	0.0
16-17	0.0	3.465298843907E-5	0.0	1.55938447975815E-4	0.0
18-19	0.0	3.465298843907E-5	0.0	1.55938447975815E-4	0.0
20-21	0.0	4.33162355488375E-5	0.0	1.646016950855825E-4	0.0
22-23	1.7326494219535E-5	6.06427297683725E-5	0.0	1.992546835246525E-4	0.0
24-25	3.465298843907E-5	6.930597687814E-5	0.0	3.465298843907E-4	0.0
26-27	3.465298843907E-5	6.930597687814E-5	0.0	5.804375563544224E-4	0.0
28-29	3.465298843907E-5	6.930597687814E-5	0.0	0.0010655793945014023	0.0
30-31	3.465298843907E-5	6.930597687814E-5	0.0	0.002746249333796297	0.0
32-33	3.465298843907E-5	7.79692239879075E-5	0.0	0.0069305976878139995	0.0
34-35	3.465298843907E-5	8.6632471097675E-5	0.0	0.013644614197883813	0.0
36-37	3.465298843907E-5	8.6632471097675E-5	0.0	0.022992257829322946	0.0
38-39	3.465298843907E-5	1.0395896531720999E-4	0.0	0.03502550806479	0.0
40-41	3.465298843907E-5	1.0395896531720999E-4	0.0	0.04638302502569519	0.0
42-43	3.465298843907E-5	1.1262221242697749E-4	0.0	0.058849437616650624	0.0
44-45	3.465298843907E-5	1.299487066465125E-4	0.0	0.07537891310208701	0.0
46-47	3.465298843907E-5	1.7326494219535E-4	0.0	0.09474127039241736	0.0
48-49	3.465298843907E-5	2.4257091907348998E-4	0.0	0.11522118655990773	0.0
50-51	3.465298843907E-5	3.205401430613975E-4	0.0	0.13531991985456834	0.0
52-53	3.465298843907E-5	3.725196257200025E-4	0.0	0.15618101889488847	0.0
54-55	3.465298843907E-5	3.98509367049305E-4	0.0	0.17951980660860212	0.0
56-57	3.465298843907E-5	4.1583586126883997E-4	0.0	0.2071122486532116	0.0
58-59	3.465298843907E-5	4.1583586126883997E-4	0.0	0.23798806135242295	0.0
60-61	3.465298843907E-5	4.1583586126883997E-4	0.0	0.2686559561209999	0.0
62-63	3.465298843907E-5	4.1583586126883997E-4	0.0	0.30015552261211453	0.0
64-65	3.465298843907E-5	4.1583586126883997E-4	0.0	0.3353283058777706	0.0
66-67	3.465298843907E-5	4.1583586126883997E-4	0.0	0.37796014490493646	0.0
68-69	3.465298843907E-5	4.1583586126883997E-4	0.0	0.426266410789	0.0
70-71	3.465298843907E-5	4.3316235548837497E-4	0.0	0.4795367172669604	0.0
72-73	3.465298843907E-5	4.3316235548837497E-4	0.0	0.5339592356105198	0.0
74-75	3.465298843907E-5	4.5915209681767746E-4	0.0	0.5928779792040486	0.0
76-77	3.465298843907E-5	4.7647859103721246E-4	0.0	0.6566914574145959	0.0
78-79	6.930597687814E-5	5.02468332366515E-4	0.0	0.7314899329603286	0.0
80-81	1.0395896531720999E-4	5.111315794762825E-4	0.0	0.814977645357158	0.0
82-83	1.0395896531720999E-4	5.1979482658605E-4	0.0	0.904477651248166	0.0
84-85	1.0395896531720999E-4	5.1979482658605E-4	0.0	0.9951471954989928	0.0
86-87	1.0395896531720999E-4	5.1979482658605E-4	0.0	1.0960566978335644	0.0
88	1.0395896531720999E-4	5.1979482658605E-4	0.0	1.1778550770439893	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6540	0.0	43.142464	1
GTATCAA	10790	0.0	34.604336	1
TCAACGC	11365	0.0	32.091522	4
ATCAACG	11445	0.0	31.868309	3
CAACGCA	12325	0.0	29.7836	5
AACGCAG	13165	0.0	28.02532	6
TATCAAC	13155	0.0	27.868708	2
ACGCAGA	15030	0.0	24.329315	7
CGCAGAG	14975	0.0	24.16507	8
GTACATG	17875	0.0	23.440298	1
TACATGG	17995	0.0	22.567059	2
ACATGGG	18125	0.0	21.393854	3
GTGGTAT	3685	0.0	19.907583	1
GCAGAGT	17825	0.0	19.24692	9
CATGGGG	12065	0.0	18.698723	4
AGAGTAC	15300	0.0	17.431879	10-11
CAGAGTA	16820	0.0	17.281582	10-11
GTACCTG	4505	0.0	17.223467	1
GAGTACA	13555	0.0	16.65226	1
TACCTGG	4380	0.0	16.418383	2
>>END_MODULE