FastQCFastQC Report
Wed 25 May 2016
SRR2049448_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049448_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5771508
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193600.3354409280901976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178020.30844625009616206No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104850.18166829189182446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105950.058.3681951
GTATCAA195450.040.4534451
ATCAACG238850.032.432973
TCAACGC241550.032.0898974
TATCAAC248400.031.4699022
CAACGCA251400.030.8886935
GTGGTAT45950.030.5219751
AACGCAG264300.029.607666
TGGTATC47900.026.7905672
ACGCAGA298650.026.0763637
CGCAGAG299250.025.851328
GTACATG172650.023.8519211
GCAGAGT334350.022.8422589
TACATGG177000.022.3611222
ACATGGG178650.021.2863033
CAGAGTA324600.020.76288410-11
GAGTACT236500.020.75698712-13
AGAGTAC308950.020.28578810-11
AGTACTT246200.018.97515712-13
GTACTTT260350.018.72912814-15
Produced by FastQC (version 0.11.5)