##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049448_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5771508
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.97197023724129	33.0	31.0	34.0	30.0	34.0
2	32.153861347848775	33.0	31.0	34.0	30.0	34.0
3	32.179891979704436	34.0	31.0	34.0	30.0	34.0
4	35.84001027114577	37.0	35.0	37.0	35.0	37.0
5	35.88933498836006	37.0	35.0	37.0	35.0	37.0
6	35.959200264471605	37.0	35.0	37.0	35.0	37.0
7	35.921956791881776	37.0	35.0	37.0	35.0	37.0
8	35.94224334437378	37.0	35.0	37.0	35.0	37.0
9	37.66887042346645	39.0	37.0	39.0	35.0	39.0
10-11	37.639936217709476	39.0	37.0	39.0	35.0	39.0
12-13	37.61829248092526	39.0	37.0	39.0	35.0	39.0
14-15	38.92238354343441	40.0	38.0	41.0	35.0	41.0
16-17	38.93355999853071	40.0	38.0	41.0	35.5	41.0
18-19	39.00954464587072	40.0	38.5	41.0	36.0	41.0
20-21	39.03407367710484	40.0	39.0	41.0	35.5	41.0
22-23	39.00435154902324	40.0	39.0	41.0	35.0	41.0
24-25	38.98289181960763	40.0	39.0	41.0	35.0	41.0
26-27	38.88637778895914	40.0	38.0	41.0	35.0	41.0
28-29	38.80567669662764	40.0	38.0	41.0	35.0	41.0
30-31	38.697149514476976	40.0	38.0	41.0	35.0	41.0
32-33	38.563317160783626	40.0	38.0	41.0	35.0	41.0
34-35	38.446879914226926	40.0	38.0	41.0	34.0	41.0
36-37	38.317229483178394	40.0	38.0	41.0	34.0	41.0
38-39	38.21423646991393	40.0	38.0	41.0	34.0	41.0
40-41	38.12815454817008	40.0	38.0	41.0	34.0	41.0
42-43	37.989266496728405	40.0	38.0	41.0	33.0	41.0
44-45	37.81660183092529	40.0	38.0	41.0	33.0	41.0
46-47	37.56786770459298	40.0	37.0	41.0	33.0	41.0
48-49	37.322416862282786	40.0	37.0	41.0	32.5	41.0
50-51	37.166189841545744	40.0	37.0	41.0	32.0	41.0
52-53	37.05795296480573	40.0	37.0	41.0	32.0	41.0
54-55	36.91733035802775	40.0	36.0	41.0	31.5	41.0
56-57	36.773795860631225	40.0	36.0	41.0	31.0	41.0
58-59	36.599995703029435	39.0	35.5	41.0	31.0	41.0
60-61	36.415412575015054	39.0	35.0	41.0	31.0	41.0
62-63	36.19752099451305	39.0	35.0	41.0	31.0	41.0
64-65	35.97907150089716	38.5	35.0	40.5	30.5	41.0
66-67	35.719070561801175	38.0	35.0	40.0	30.0	41.0
68-69	35.429916496693764	37.0	35.0	40.0	30.0	41.0
70-71	35.146043893554335	37.0	35.0	40.0	29.5	41.0
72-73	34.81319457583703	36.5	34.5	39.0	29.0	41.0
74-75	34.490824061926276	36.0	34.0	39.0	29.0	41.0
76-77	34.16736189224723	35.5	34.0	38.5	29.0	40.0
78-79	33.85824683947419	35.0	34.0	37.0	29.0	39.5
80-81	33.544519473939914	35.0	34.0	37.0	29.0	39.0
82-83	33.2669132573324	35.0	34.0	36.5	29.0	39.0
84-85	32.979901699867696	35.0	34.0	36.0	29.0	37.5
86-87	32.73384226444804	35.0	34.0	36.0	28.5	37.0
88-89	32.51516839273202	35.0	34.0	35.5	28.0	37.0
90-91	32.32356197028575	35.0	33.5	35.0	27.0	36.0
92-93	32.160985482477024	35.0	33.0	35.0	27.0	36.0
94-95	32.001880097887764	35.0	33.0	35.0	27.0	36.0
96-97	31.85834560049124	35.0	33.0	35.0	27.0	36.0
98-99	31.723307669330097	35.0	33.0	35.0	26.5	35.5
100	31.61575172381291	35.0	33.0	35.0	26.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.279931311777581
1101	2	0.33485404274593833
1101	3	0.4353390809815636
1101	4	0.2900276513496465
1101	5	0.26693153988977514
1101	6	0.23229573163244766
1101	7	0.23620443884701814
1101	8	0.24650880722172275
1101	9	0.34726285002664525
1101	10-11	0.35100819560037877
1101	12-13	0.3257494844916664
1101	14-15	0.4193225377799976
1101	16-17	0.39899371961292474
1101	18-19	0.42757637159488837
1101	20-21	0.44189329036963443
1101	22-23	0.3668390301716187
1101	24-25	0.41276150035248094
1101	26-27	0.40260883841074246
1101	28-29	0.4550212755876899
1101	30-31	0.4346617058552198
1101	32-33	0.4138006140651953
1101	34-35	0.3575705134833953
1101	36-37	0.3938494666414911
1101	38-39	0.37192081388155174
1101	40-41	0.3608021686247511
1101	42-43	0.36621193824215936
1101	44-45	0.3638015040224545
1101	46-47	0.31549229092711784
1101	48-49	0.30932404598842567
1101	50-51	0.25035007091624806
1101	52-53	0.27440625597478174
1101	54-55	0.2590207129002735
1101	56-57	0.31281471245777936
1101	58-59	0.31600719415966694
1101	60-61	0.23946736729836715
1101	62-63	0.24729163144036193
1101	64-65	0.20816160192361366
1101	66-67	0.2984359595684154
1101	68-69	0.2700237486154151
1101	70-71	0.2422135353705741
1101	72-73	0.32307200490735255
1101	74-75	0.32151020833899224
1101	76-77	0.3184057975705912
1101	78-79	0.2656987479494788
1101	80-81	0.22850498487132143
1101	82-83	0.21259179191234523
1101	84-85	0.19840996891471008
1101	86-87	0.1173994316747482
1101	88-89	0.14626531437164658
1101	90-91	0.21022195326861493
1101	92-93	0.22858739206181866
1101	94-95	0.2188695053872891
1101	96-97	0.15439745050812448
1101	98-99	0.2053998085465274
1101	100	0.22325288741915728
1102	1	0.2568073122754839
1102	2	0.2308288288602327
1102	3	0.34708405640746065
1102	4	0.22353369047547744
1102	5	0.2033297708488604
1102	6	0.1808613890566093
1102	7	0.20169853758244471
1102	8	0.22268976282772002
1102	9	0.22573670256597467
1102	10-11	0.22817657404423386
1102	12-13	0.23839503351405966
1102	14-15	0.33677475982227634
1102	16-17	0.3310462260107627
1102	18-19	0.31737483149733947
1102	20-21	0.28404880957116063
1102	22-23	0.2554532719748934
1102	24-25	0.24840473183356693
1102	26-27	0.2649477549016268
1102	28-29	0.2739223290216444
1102	30-31	0.318065470002729
1102	32-33	0.2890889264427301
1102	34-35	0.3087761971145824
1102	36-37	0.30532295244312735
1102	38-39	0.33187763352958655
1102	40-41	0.3244091158279119
1102	42-43	0.2743099482217062
1102	44-45	0.27393248224841216
1102	46-47	0.3234487087029194
1102	48-49	0.2671568821958772
1102	50-51	0.22311993933237062
1102	52-53	0.22866197755127615
1102	54-55	0.294555236435734
1102	56-57	0.2976155347399114
1102	58-59	0.30027302611885176
1102	60-61	0.2530613063556615
1102	62-63	0.1879013646453842
1102	64-65	0.19186097304877592
1102	66-67	0.19630192932191903
1102	68-69	0.1829765241103729
1102	70-71	0.14958344889274855
1102	72-73	0.22726889285694085
1102	74-75	0.15881128827442126
1102	76-77	0.13051183897179186
1102	78-79	0.1851861215989743
1102	80-81	0.21895487264156088
1102	82-83	0.18409451634510532
1102	84-85	0.16362427121632805
1102	86-87	0.1858165318905094
1102	88-89	0.14953267618916755
1102	90-91	0.1698721236544145
1102	92-93	0.14971883640603068
1102	94-95	0.15791890522238816
1102	96-97	0.1736029949396034
1102	98-99	0.2367929901700201
1102	100	0.23827713694534225
1103	1	0.2217475649668259
1103	2	0.228244857458769
1103	3	0.33107100055195815
1103	4	0.2175845232361837
1103	5	0.1821301107428468
1103	6	0.17334389331039546
1103	7	0.2035570132540485
1103	8	0.1727246316147344
1103	9	0.2327587752239353
1103	10-11	0.2027393593343092
1103	12-13	0.1608701396542358
1103	14-15	0.27811889020904346
1103	16-17	0.28698629259888975
1103	18-19	0.2650996431445307
1103	20-21	0.24303337629835653
1103	22-23	0.27753014793609054
1103	24-25	0.26457180854080775
1103	26-27	0.26644885106994565
1103	28-29	0.32537743622527415
1103	30-31	0.3409252320360423
1103	32-33	0.2970807552850445
1103	34-35	0.33634298975880483
1103	36-37	0.3174812690067128
1103	38-39	0.2886647508932185
1103	40-41	0.2853296160160568
1103	42-43	0.2975074057656357
1103	44-45	0.26071174135315545
1103	46-47	0.2774692025512806
1103	48-49	0.27441708003789955
1103	50-51	0.28438841160912176
1103	52-53	0.23983622229948764
1103	54-55	0.2320682738854245
1103	56-57	0.21120523546142067
1103	58-59	0.2444136894642952
1103	60-61	0.173283617880152
1103	62-63	0.17332996952578839
1103	64-65	0.13067532692299721
1103	66-67	0.14829305465323017
1103	68-69	0.1457437407017821
1103	70-71	0.17810823845297108
1103	72-73	0.1820818591374902
1103	74-75	0.160898430588162
1103	76-77	0.14558440899568126
1103	78-79	0.19117746882255915
1103	80-81	0.15488564940770289
1103	82-83	0.22159712383504626
1103	84-85	0.1665345623144603
1103	86-87	0.06194861710532251
1103	88-89	0.1415453209815496
1103	90-91	0.12173709248723696
1103	92-93	0.10720690841774427
1103	94-95	0.11594445248850604
1103	96-97	0.10214243245800603
1103	98-99	0.10614291736481363
1103	100	0.17840277705855812
1104	1	0.2622980864404312
1104	2	0.21857508735953246
1104	3	0.3352418347481745
1104	4	0.20349148047696275
1104	5	0.17277373152189313
1104	6	0.16557273584607657
1104	7	0.13362467410600232
1104	8	0.164304018830002
1104	9	0.18548154895535163
1104	10-11	0.21126237033951867
1104	12-13	0.21656756562642698
1104	14-15	0.27088262690048737
1104	16-17	0.26715780204504824
1104	18-19	0.2492085959526804
1104	20-21	0.23751915639380172
1104	22-23	0.2227257115549932
1104	24-25	0.2620583476039613
1104	26-27	0.23551573018882266
1104	28-29	0.24710714641489773
1104	30-31	0.20007911889073426
1104	32-33	0.24293922230978637
1104	34-35	0.2230906038022411
1104	36-37	0.21343054044177023
1104	38-39	0.180344828926998
1104	40-41	0.17338637593258
1104	42-43	0.12302859480771389
1104	44-45	0.1487949843594123
1104	46-47	0.18192436507391818
1104	48-49	0.21277784930142474
1104	50-51	0.21899225396121125
1104	52-53	0.21376318441645736
1104	54-55	0.1552601994529752
1104	56-57	0.2079025600910427
1104	58-59	0.17998762988366934
1104	60-61	0.14029321531516104
1104	62-63	0.15028692757437057
1104	64-65	0.1945238990943281
1104	66-67	0.2699365410432151
1104	68-69	0.23009210614895892
1104	70-71	0.23013589763056785
1104	72-73	0.20321518116158188
1104	74-75	0.2101179642948452
1104	76-77	0.19597305407491206
1104	78-79	0.19519431555587374
1104	80-81	0.20578100733055749
1104	82-83	0.1300765685512033
1104	84-85	0.16092785813635402
1104	86-87	0.1539845140384628
1104	88-89	0.1587021864265239
1104	90-91	0.1319086643545404
1104	92-93	0.1686604821385842
1104	94-95	0.0749165469309716
1104	96-97	0.08935137549907424
1104	98-99	0.16145438787485844
1104	100	0.13485356202284748
1105	1	0.1737869575192974
1105	2	0.17098844131617597
1105	3	0.2696912363452455
1105	4	0.20266768583778827
1105	5	0.16143945863760933
1105	6	0.1245502565723342
1105	7	0.15155515529468744
1105	8	0.129791689663044
1105	9	0.1726950605669586
1105	10-11	0.15497952058133535
1105	12-13	0.16479100990748208
1105	14-15	0.19938682783457295
1105	16-17	0.21337181173008446
1105	18-19	0.1998565114533477
1105	20-21	0.17360131295239256
1105	22-23	0.16260876938565616
1105	24-25	0.20424565457545185
1105	26-27	0.16665746970576834
1105	28-29	0.17314747270560105
1105	30-31	0.16750721858571183
1105	32-33	0.16213462783078114
1105	34-35	0.14097490037573834
1105	36-37	0.17280058470772275
1105	38-39	0.18034931680525546
1105	40-41	0.19882987484223236
1105	42-43	0.16936247432099094
1105	44-45	0.15721150261659034
1105	46-47	0.1787168364746421
1105	48-49	0.15301965736131962
1105	50-51	0.09202738495434204
1105	52-53	0.11414074688808995
1105	54-55	0.1559191585240356
1105	56-57	0.10728608331984191
1105	58-59	0.11307219327148488
1105	60-61	0.09506991249438812
1105	62-63	0.11415773893588721
1105	64-65	0.13329876050289613
1105	66-67	0.08248888304211022
1105	68-69	0.14392048090002874
1105	70-71	0.16110109182806553
1105	72-73	0.20799624536324046
1105	74-75	0.19757053464347507
1105	76-77	0.17503978438838885
1105	78-79	0.0899417942157541
1105	80-81	0.14607019235161545
1105	82-83	0.1232643083339724
1105	84-85	0.10034555467896666
1105	86-87	0.1156346068012084
1105	88-89	0.12077598206053608
1105	90-91	0.11926987063962713
1105	92-93	0.052928919808969965
1105	94-95	0.07452691950976487
1105	96-97	0.10321447566878206
1105	98-99	0.1515688153585124
1105	100	0.1072804801309637
1106	1	0.08397011157485679
1106	2	0.11389211658604381
1106	3	0.2200229721274809
1106	4	0.1445389427906747
1106	5	0.12269666583657113
1106	6	0.09405095281320541
1106	7	0.10415952784549631
1106	8	0.10898293894791067
1106	9	0.10387732650311676
1106	10-11	0.1274217890806213
1106	12-13	0.10923676591564657
1106	14-15	0.18307977460504077
1106	16-17	0.20117917979427347
1106	18-19	0.17532689167060056
1106	20-21	0.15898960107465143
1106	22-23	0.16067424060434377
1106	24-25	0.1463903143862595
1106	26-27	0.09714023208976386
1106	28-29	0.10410051023137612
1106	30-31	0.095253808138132
1106	32-33	0.07907438509714382
1106	34-35	0.041858980598703965
1106	36-37	0.10151731665060737
1106	38-39	0.02565964817630828
1106	40-41	0.03807053311487607
1106	42-43	0.07809777363689108
1106	44-45	0.13198309165787947
1106	46-47	0.09587901895877593
1106	48-49	0.1155452859852204
1106	50-51	0.07469693209039718
1106	52-53	0.051269594440796595
1106	54-55	0.09200634824056664
1106	56-57	0.10918709480142041
1106	58-59	0.05158463210775466
1106	60-61	0.023164576176959883
1106	62-63	0.08585528936625053
1106	64-65	0.04116930636187277
1106	66-67	0.024288204247334022
1106	68-69	0.04973752403020626
1106	70-71	0.08211616805908051
1106	72-73	0.11115159459249213
1106	74-75	0.11181362746174983
1106	76-77	0.0821905239737859
1106	78-79	0.1220324822692831
1106	80-81	0.09806693650747889
1106	82-83	0.08034229650098013
1106	84-85	0.09627309946659324
1106	86-87	0.10339775055152955
1106	88-89	0.07054790413769751
1106	90-91	0.07311028711754375
1106	92-93	0.06928970045074578
1106	94-95	0.04101905875907619
1106	96-97	0.05828017919334272
1106	98-99	0.08221018117743739
1106	100	0.04757365285291115
1107	1	0.08657201142842297
1107	2	0.1105492322927546
1107	3	0.18968367391288865
1107	4	0.08959295136769185
1107	5	0.12740451129556618
1107	6	0.10842792538596058
1107	7	0.0660565600912193
1107	8	0.07964202281905841
1107	9	0.08263699642735389
1107	10-11	0.09521268634427571
1107	12-13	0.06916399117578464
1107	14-15	0.11356980117091098
1107	16-17	0.10885990043038163
1107	18-19	0.10377363117964222
1107	20-21	0.12436560792313145
1107	22-23	0.10434048552148312
1107	24-25	0.10654865645168599
1107	26-27	0.11586905101235345
1107	28-29	0.09201842511720315
1107	30-31	0.1189724601435671
1107	32-33	0.10751629356929016
1107	34-35	0.11646531147931682
1107	36-37	0.13814024794022117
1107	38-39	0.14389676416322317
1107	40-41	0.2034580378311759
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2216	68-69	-0.6355851180116474
2216	70-71	-0.6008624828155789
2216	72-73	-0.5818971089874196
2216	74-75	-0.6078335144674156
2216	76-77	-0.488308177806978
2216	78-79	-0.47790180189205955
2216	80-81	-0.4643186052504831
2216	82-83	-0.5157959984097431
2216	84-85	-0.5643913312202429
2216	86-87	-0.45646729525070384
2216	88-89	-0.5818054614790924
2216	90-91	-0.5247851016001661
2216	92-93	-0.48259248070906935
2216	94-95	-0.5438469813029698
2216	96-97	-0.5231835985015252
2216	98-99	-0.5876398844437105
2216	100	-0.5848009615163221
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	457.0
3	41.0
4	54.0
5	137.0
6	459.0
7	1077.0
8	2352.0
9	3725.0
10	4825.0
11	6057.0
12	8900.0
13	12851.0
14	15070.0
15	19383.0
16	24571.0
17	34592.0
18	36426.0
19	19275.0
20	13535.0
21	13029.0
22	13556.0
23	14683.0
24	17310.0
25	21076.0
26	27015.0
27	35307.0
28	46512.0
29	60771.0
30	78613.0
31	102225.0
32	133651.0
33	175984.0
34	243209.0
35	362141.0
36	601663.0
37	1156210.0
38	1733043.0
39	729484.0
40	2239.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.524421936337184	20.679554855762525	14.937240685855768	22.85878252204452
2	15.272715553716637	21.006121796937645	40.05648090585684	23.664681743488877
3	17.586391632828025	26.229574662289302	31.223919294576046	24.96011441030663
4	12.45941268729074	17.99605926215471	38.73145458691212	30.813073463642432
5	12.378421559725236	37.86189340745413	35.679667842710494	14.080017190110143
6	29.352188034143605	38.82420616459658	17.90574555510218	13.917860246157632
7	25.611573266466927	33.37952576692261	23.06608602119238	17.94281494541808
8	24.62853729042739	35.86859794701836	20.74702140237872	18.75584336017554
9	24.640301980002455	16.706881459750207	22.14010619061777	36.512710369629566
10-11	23.25814154636882	27.275531802087084	29.219729055213996	20.24659759633011
12-13	24.191008658395692	24.96163914179795	29.467030107209414	21.38032209259694
14-15	21.469198344696046	26.87724767946263	27.380357092115265	24.273196883726055
16-17	20.324705432271774	30.053046794702524	27.995101107024368	21.62714666600133
18-19	20.22679341343718	28.276604658609156	31.13267797601597	20.36392395193769
20-21	21.257754472487953	27.472022909783718	31.01183434208183	20.258388275646503
22-23	20.933939622019064	27.382678842340685	31.078879211464315	20.604502324175936
24-25	20.74029005937443	27.462441358480316	31.00954724484485	20.787721337300407
26-27	20.597155890626855	27.477593377675298	31.025504946021037	20.89974578567681
28-29	20.62880273231883	27.407594341028375	31.218504765132437	20.74509816152035
30-31	20.887331352568513	27.785207956048925	30.730148862307736	20.597311829074826
32-33	20.57641607704607	27.572187372866846	31.100866532628906	20.75053001745818
34-35	20.767761215959503	27.40146942532177	30.97441777781474	20.856351580903986
36-37	21.077073790766644	27.566426313538855	30.543889049447735	20.81261084624677
38-39	20.890285519832947	27.77255095202155	30.624214676649498	20.712948851496005
40-41	20.83116752155589	27.505670961558053	30.675293181608687	20.987868335277366
42-43	20.823639159817503	27.627069043307223	30.45085443873594	21.09843735813933
44-45	21.036001336219236	27.762709503304855	30.203674672200055	20.997614488275854
46-47	21.183146588378634	27.932604442374505	29.781402018328656	21.1028469509182
48-49	21.17804393583098	27.89634008997302	29.576126378062717	21.34948959613328
50-51	21.209872705712268	27.951386362108483	29.381965683838608	21.45677524834064
52-53	21.215954305183324	28.03976880912233	29.293063441998175	21.451213443696172
54-55	21.344846095682446	28.317945673816965	29.168719856231682	21.16848837426891
56-57	21.220901019283005	28.093584900168207	29.15544776165952	21.530066318889276
58-59	21.145175576296523	27.774647457822116	29.502332839181715	21.57784412669964
60-61	21.315858870853162	28.053049566941603	29.211273726034857	21.419817836170374
62-63	21.200577041563488	28.04358930109774	29.316315597240788	21.439518060097985
64-65	21.230032081736695	27.908745859834205	29.369620556707186	21.491601501721906
66-67	21.105246670367606	27.960664699763043	29.306924637373804	21.62716399249555
68-69	21.409586541333738	28.12181842249894	29.118360400782606	21.35023463538472
70-71	21.44642266804447	27.838313660831798	29.18897452797432	21.526289143149416
72-73	21.198896371624194	28.014957269399954	29.28358584966009	21.502560509315764
74-75	21.304988341434193	28.035898766483452	29.109249186629317	21.54986370545304
76-77	21.402188128301997	28.070800560269515	28.91771959772039	21.6092917137081
78-79	21.425015784436233	28.117919961299542	29.03839862995945	21.418665624304776
80-81	21.33623344645819	28.00334020676431	29.091489056829083	21.568937289948416
82-83	21.24709335644007	28.04212232346437	29.232381003370627	21.478403316724933
84-85	21.241523849655408	27.97361789541969	29.106350043524305	21.678508211400597
86-87	21.388733912272066	28.11608980712053	28.934675334576227	21.560500946031176
88-89	21.226343989886807	28.018675305017503	29.00780771352612	21.74717299156957
90-91	21.1272149602956	27.983456882296835	29.04790093535201	21.841427222055547
92-93	21.224060769701854	28.143862911070954	28.924995077098316	21.707081242128883
94-95	21.099852325905964	27.949535761370587	29.112804953588267	21.837806959135182
96-97	21.134736412587948	28.037298973528852	29.071671051018612	21.756293562864588
98-99	21.0790707619141	28.116008523352416	28.919378498045027	21.885542216688457
100	21.186173438447543	28.0142770510266	28.916278263882873	21.883271246642988
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1624.0
1	1137.5
2	1406.0
3	2141.5
4	1363.5
5	749.0
6	1811.5
7	4615.5
8	7778.5
9	8996.0
10	6780.5
11	4083.5
12	3723.0
13	4100.0
14	4611.0
15	5178.5
16	5802.0
17	6528.5
18	7300.5
19	8238.0
20	9693.0
21	11840.0
22	15013.5
23	20098.0
24	26623.5
25	34691.0
26	46215.5
27	60684.5
28	78422.5
29	97133.5
30	113189.0
31	129403.0
32	145192.0
33	158156.5
34	169804.5
35	179903.5
36	188285.5
37	196417.5
38	202994.5
39	205875.5
40	208203.0
41	208816.5
42	205645.5
43	201696.5
44	197353.5
45	193052.0
46	188560.5
47	181544.5
48	175234.5
49	171696.0
50	167306.0
51	159606.0
52	147278.0
53	137497.0
54	132084.5
55	126194.0
56	117574.0
57	105495.5
58	94790.5
59	86222.0
60	75645.0
61	63776.0
62	53318.5
63	43699.0
64	35108.5
65	28306.0
66	22371.5
67	17395.0
68	13490.0
69	10205.0
70	7649.5
71	5638.0
72	4010.5
73	2735.5
74	1933.0
75	1347.5
76	834.5
77	551.5
78	365.0
79	221.0
80	124.5
81	56.0
82	30.5
83	19.5
84	11.5
85	5.5
86	3.0
87	3.0
88	3.5
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12585965401070223
2	0.0
3	0.0
4	0.0
5	0.01296021767621218
6	5.1979482658604996E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	8.663247109767498E-6
76-77	0.0
78-79	0.0
80-81	1.55938447975815E-4
82-83	5.457845679153524E-4
84-85	3.465298843907E-4
86-87	4.8514183814697996E-4
88-89	6.2375379190326E-4
90-91	7.710289927693074E-4
92-93	2.3390767196372248E-4
94-95	2.59897413293025E-4
96-97	6.150905447934924E-4
98-99	0.00121285459536745
100	1.3861195375627997E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5771508.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.97191150839218
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.35190966890292	43.103223278056085
2	15.380510499154996	17.832751872218203
3	4.715085704169228	8.200275935897507
4	1.9295426247364418	4.474370971715672
5	1.0257903244502977	2.9733512957598784
6	0.5919393456806182	2.0589513219679425
7	0.3962594749016562	1.6080343449352539
8	0.28643255137779344	1.328403401727715
9	0.21298616421663832	1.1112493558030958
>10	1.0254318445690576	10.514436195466024
>50	0.054860085479921	2.1879857521420685
>100	0.027426823017635535	2.8234765006674194
>500	0.0013462349199674173	0.5170130075215171
>1k	3.889067186705246E-4	0.44014342634592873
>5k	0.0	0.0
>10k+	8.97477042696462E-5	0.8263333397755035
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19360	0.3354409280901976	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17802	0.30844625009616206	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10485	0.18166829189182446	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	1.7326494219535E-5	0.0	0.0	0.0
4	0.0	1.7326494219535E-5	0.0	6.930597687814E-5	0.0
5	0.0	1.7326494219535E-5	0.0	6.930597687814E-5	0.0
6	0.0	1.7326494219535E-5	0.0	6.930597687814E-5	0.0
7	0.0	1.7326494219535E-5	0.0	6.930597687814E-5	0.0
8	0.0	1.7326494219535E-5	0.0	6.930597687814E-5	0.0
9	0.0	1.7326494219535E-5	0.0	8.6632471097675E-5	0.0
10-11	0.0	1.7326494219535E-5	0.0	8.6632471097675E-5	0.0
12-13	0.0	1.7326494219535E-5	0.0	8.6632471097675E-5	0.0
14-15	0.0	1.7326494219535E-5	0.0	8.6632471097675E-5	0.0
16-17	0.0	1.7326494219535E-5	0.0	1.7326494219535E-4	0.0
18-19	0.0	1.7326494219535E-5	0.0	1.819281893051175E-4	0.0
20-21	8.6632471097675E-6	1.7326494219535E-5	0.0	1.992546835246525E-4	0.0
22-23	1.7326494219535E-5	1.7326494219535E-5	0.0	2.339076719637225E-4	0.0
24-25	1.7326494219535E-5	2.59897413293025E-5	0.0	3.8118287282977E-4	0.0
26-27	1.7326494219535E-5	3.465298843907E-5	0.0	6.324170390130275E-4	0.0
28-29	1.7326494219535E-5	3.465298843907E-5	0.0	0.0011348853713795423	0.0
30-31	2.59897413293025E-5	3.465298843907E-5	0.0	0.00284154505200374	0.0
32-33	4.33162355488375E-5	3.465298843907E-5	0.0	0.007008566911801907	0.0
34-35	5.1979482658604996E-5	3.465298843907E-5	0.0	0.013748573163201022	0.0
36-37	6.06427297683725E-5	4.33162355488375E-5	0.0	0.023295471478164805	0.0
38-39	7.79692239879075E-5	6.930597687814E-5	0.0	0.03544134392605884	0.0
40-41	8.6632471097675E-5	6.930597687814E-5	0.0	0.046989452323378915	0.0
42-43	8.6632471097675E-5	6.930597687814E-5	0.0	0.059646456350749236	0.0
44-45	8.6632471097675E-5	6.930597687814E-5	0.0	0.07616726858907585	0.0
46-47	8.6632471097675E-5	6.930597687814E-5	0.0	0.09559893185628435	0.0
48-49	8.6632471097675E-5	8.6632471097675E-5	0.0	0.11607884802377472	0.0
50-51	8.6632471097675E-5	1.0395896531720999E-4	0.0	0.13625555054242322	0.0
52-53	8.6632471097675E-5	1.2128545953674499E-4	0.0	0.15728125127782894	0.0
54-55	8.6632471097675E-5	1.2128545953674499E-4	0.0	0.180568059508884	0.0
56-57	8.6632471097675E-5	1.2128545953674499E-4	0.0	0.20837708273123767	0.0
58-59	8.6632471097675E-5	1.3861195375628E-4	0.0	0.23950412959663228	0.0
60-61	8.6632471097675E-5	1.3861195375628E-4	0.0	0.27031929956607526	0.0
62-63	1.0395896531720999E-4	1.3861195375628E-4	0.0	0.3019228250225071	0.0
64-65	1.299487066465125E-4	1.472752008660475E-4	0.0	0.33733817920723663	0.0
66-67	1.3861195375628E-4	1.55938447975815E-4	0.0	0.37982274303353647	0.0
68-69	1.3861195375628E-4	1.646016950855825E-4	0.0	0.42805970294072193	0.0
70-71	1.3861195375628E-4	1.7326494219535E-4	0.0	0.4810961017467186	0.0
72-73	1.3861195375628E-4	1.7326494219535E-4	0.0	0.5354926303489487	0.0
74-75	1.472752008660475E-4	2.0791793063441999E-4	0.0	0.5945673123904532	0.0
76-77	1.55938447975815E-4	2.0791793063441999E-4	0.0	0.6583894538481104	0.0
78-79	1.55938447975815E-4	2.0791793063441999E-4	0.0	0.7334564900542457	0.0
80-81	1.55938447975815E-4	2.0791793063441999E-4	0.0	0.8170481614163924	0.0
82-83	1.55938447975815E-4	2.0791793063441999E-4	0.0	0.9067560852380349	0.0
84-85	1.55938447975815E-4	2.0791793063441999E-4	0.0	0.9977634961261423	0.0
86-87	1.55938447975815E-4	2.0791793063441999E-4	0.0	1.098716314696263	0.0
88	1.55938447975815E-4	2.0791793063441999E-4	0.0	1.1811297844514812	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10595	0.0	58.368195	1
GTATCAA	19545	0.0	40.453445	1
ATCAACG	23885	0.0	32.43297	3
TCAACGC	24155	0.0	32.089897	4
TATCAAC	24840	0.0	31.469902	2
CAACGCA	25140	0.0	30.888693	5
GTGGTAT	4595	0.0	30.521975	1
AACGCAG	26430	0.0	29.60766	6
TGGTATC	4790	0.0	26.790567	2
ACGCAGA	29865	0.0	26.076363	7
CGCAGAG	29925	0.0	25.85132	8
GTACATG	17265	0.0	23.851921	1
GCAGAGT	33435	0.0	22.842258	9
TACATGG	17700	0.0	22.361122	2
ACATGGG	17865	0.0	21.286303	3
CAGAGTA	32460	0.0	20.762884	10-11
GAGTACT	23650	0.0	20.756987	12-13
AGAGTAC	30895	0.0	20.285788	10-11
AGTACTT	24620	0.0	18.975157	12-13
GTACTTT	26035	0.0	18.729128	14-15
>>END_MODULE