FastQCFastQC Report
Wed 25 May 2016
SRR2049447_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049447_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5132925
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58980.11490524408597437No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59200.046.638741
GTATCAA100600.033.196381
TCAACGC107200.030.4688684
ATCAACG107200.030.3382343
CAACGCA114900.028.4690155
AACGCAG121850.027.0008166
TATCAAC122900.026.768572
TACCTGG42850.025.5554832
GTACCTG46850.024.7980631
GTGGTAT32350.024.4267331
GTACATG163850.023.0515171
ACGCAGA142650.022.5700057
TACATGG164100.022.1958662
CGCAGAG143450.021.950098
ACATGGG164900.020.6346423
TGGTATC35000.019.8734232
ACCTGGG56550.018.7825623
GCAGAGT162900.018.5223989
TCTGTCG13600.017.6235478
TAGACCG2152.625975E-517.4877825
Produced by FastQC (version 0.11.5)