FastQCFastQC Report
Wed 25 May 2016
SRR2049447_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049447_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5132925
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190780.3716789160176702No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189670.3695164063375171No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110070.21443913558059002No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55260.10765791434708281No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111300.059.2084051
GTATCAA198200.039.8747061
GTGGTAT44650.037.8463061
TGGTATC45100.036.6883432
ATCAACG247750.031.609913
TCAACGC251400.031.3005624
TATCAAC255500.030.7614842
CAACGCA260850.030.1485925
AACGCAG271400.029.0757486
ACGCAGA304350.025.8041137
CGCAGAG307100.025.40478
GCGGTAT3350.025.291711
GCAGAGT331800.023.2018899
GTACATG153600.022.6159931
TACCTGG38250.021.9980182
GAGTACT248400.021.5410712-13
CAGAGTA325450.021.3801110-11
TACATGG156650.021.3354192
GTACCTG43500.020.9924411
ACATGGG156000.020.7915293
Produced by FastQC (version 0.11.5)