##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049447_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5132925 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.88720953452466 33.0 31.0 34.0 30.0 34.0 2 31.973478864390188 33.0 31.0 34.0 30.0 34.0 3 31.998380845229573 33.0 31.0 34.0 30.0 34.0 4 35.747716360554655 37.0 35.0 37.0 33.0 37.0 5 35.82625442608259 37.0 35.0 37.0 35.0 37.0 6 35.91621950447357 37.0 35.0 37.0 35.0 37.0 7 35.91109279796607 37.0 35.0 37.0 35.0 37.0 8 35.93455972958888 37.0 35.0 37.0 35.0 37.0 9 37.65354237593575 39.0 37.0 39.0 35.0 39.0 10-11 37.63496895045223 39.0 37.0 39.0 35.0 39.0 12-13 37.624334468163866 39.0 37.0 39.0 35.0 39.0 14-15 38.91973358270382 40.0 38.0 41.0 35.0 41.0 16-17 38.934157814501475 40.0 38.0 41.0 35.5 41.0 18-19 39.01706074022122 40.0 38.5 41.0 36.0 41.0 20-21 39.040901435341446 40.0 39.0 41.0 35.5 41.0 22-23 39.005048096358315 40.0 39.0 41.0 35.0 41.0 24-25 38.98312219640848 40.0 38.5 41.0 35.0 41.0 26-27 38.87971341876221 40.0 38.0 41.0 35.0 41.0 28-29 38.7919019857099 40.0 38.0 41.0 35.0 41.0 30-31 38.667829453966306 40.0 38.0 41.0 35.0 41.0 32-33 38.51162251542736 40.0 38.0 41.0 34.5 41.0 34-35 38.38052971746129 40.0 38.0 41.0 34.0 41.0 36-37 38.23534144761515 40.0 38.0 41.0 34.0 41.0 38-39 38.1231771358436 40.0 38.0 41.0 34.0 41.0 40-41 38.02319067588168 40.0 38.0 41.0 33.5 41.0 42-43 37.870185030952136 40.0 38.0 41.0 33.0 41.0 44-45 37.681056317791516 40.0 37.5 41.0 33.0 41.0 46-47 37.40575061977333 40.0 37.0 41.0 33.0 41.0 48-49 37.13446689752919 40.0 37.0 41.0 32.0 41.0 50-51 36.96686450707929 40.0 37.0 41.0 31.0 41.0 52-53 36.84849710447747 40.0 36.5 41.0 31.0 41.0 54-55 36.702914809781944 40.0 36.0 41.0 31.0 41.0 56-57 36.55377187471081 39.5 36.0 41.0 31.0 41.0 58-59 36.37518656516508 39.0 35.0 41.0 31.0 41.0 60-61 36.18334020076272 39.0 35.0 41.0 30.5 41.0 62-63 35.95721357705402 39.0 35.0 41.0 30.0 41.0 64-65 35.733308785926155 38.5 35.0 40.5 30.0 41.0 66-67 35.468799661012 38.0 35.0 40.0 29.5 41.0 68-69 35.172939308483954 37.0 35.0 40.0 29.0 41.0 70-71 34.88387293794474 37.0 35.0 39.5 29.0 41.0 72-73 34.54417744268619 36.0 34.0 39.0 29.0 41.0 74-75 34.22015566173283 36.0 34.0 39.0 29.0 41.0 76-77 33.90197587145731 35.5 34.0 38.5 28.5 40.0 78-79 33.59204323071154 35.0 34.0 37.0 28.0 39.0 80-81 33.282724957017685 35.0 34.0 37.0 28.0 39.0 82-83 33.010885216518844 35.0 34.0 36.5 28.0 39.0 84-85 32.72747877672087 35.0 34.0 36.0 27.0 37.5 86-87 32.48387771105169 35.0 34.0 36.0 27.0 37.0 88-89 32.26223459333616 35.0 33.0 35.0 27.0 37.0 90-91 32.07168797517984 35.0 33.0 35.0 26.0 36.0 92-93 31.911043800562055 35.0 33.0 35.0 26.0 36.0 94-95 31.748148765080337 35.0 33.0 35.0 26.0 36.0 96-97 31.602595888309295 35.0 33.0 35.0 25.0 36.0 98-99 31.468980941665812 35.0 33.0 35.0 25.0 35.5 100 31.360089422697584 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.35116390210673387 1101 2 0.38545605425671425 1101 3 0.45830037950194225 1101 4 0.33270291224867066 1101 5 0.2874690710726071 1101 6 0.24755176432515924 1101 7 0.2705500745287779 1101 8 0.28234637444076327 1101 9 0.34915679005620603 1101 10-11 0.3238372305787891 1101 12-13 0.3039812038499292 1101 14-15 0.4068164471789828 1101 16-17 0.40207882473391976 1101 18-19 0.39211468866070476 1101 20-21 0.42787083953123783 1101 22-23 0.3978393007921426 1101 24-25 0.42506520711862095 1101 26-27 0.4248126515597761 1101 28-29 0.4116177473108209 1101 30-31 0.40680325495358005 1101 32-33 0.41739315086818607 1101 34-35 0.3973871908632418 1101 36-37 0.4147934498667212 1101 38-39 0.3749785713217122 1101 40-41 0.43987807487741293 1101 42-43 0.3913839155064025 1101 44-45 0.41066219196991227 1101 46-47 0.418888203002318 1101 48-49 0.32980320642239747 1101 50-51 0.3195973305234645 1101 52-53 0.28702962595235704 1101 54-55 0.31819099280362195 1101 56-57 0.3423363809420934 1101 58-59 0.34774190611513234 1101 60-61 0.25546042155830406 1101 62-63 0.21116246275597206 1101 64-65 0.21393938291626569 1101 66-67 0.2606607367351472 1101 68-69 0.24041860498669365 1101 70-71 0.2706057773016326 1101 72-73 0.3431771081290762 1101 74-75 0.2436718212160045 1101 76-77 0.23062123447454042 1101 78-79 0.2581434574212196 1101 80-81 0.20416303368933342 1101 82-83 0.20753476666108384 1101 84-85 0.2000372214462871 1101 86-87 0.1457210113450813 1101 88-89 0.20897232658508358 1101 90-91 0.24572946608851254 1101 92-93 0.2229847152418145 1101 94-95 0.244280830726602 1101 96-97 0.17006861719998057 1101 98-99 0.2130963557547716 1101 100 0.21094845540084606 1102 1 0.17826996872046408 1102 2 0.20590252129031938 1102 3 0.31306969478676194 1102 4 0.21262861102662356 1102 5 0.2275364659924648 1102 6 0.1773178796299888 1102 7 0.21894020186656604 1102 8 0.17664715868004066 1102 9 0.2553883805327928 1102 10-11 0.2579190136710423 1102 12-13 0.2643483165204046 1102 14-15 0.3319737238392264 1102 16-17 0.3166828929665684 1102 18-19 0.3189810951936991 1102 20-21 0.33563712455924133 1102 22-23 0.26469329551522236 1102 24-25 0.27619669776611033 1102 26-27 0.22275369576251336 1102 28-29 0.22411721183893007 1102 30-31 0.19060673831385344 1102 32-33 0.19389100923766733 1102 34-35 0.20196005436469022 1102 36-37 0.19944279674523813 1102 38-39 0.1584798450593965 1102 40-41 0.23199767222311607 1102 42-43 0.13331737425724555 1102 44-45 0.11820519378932204 1102 46-47 0.07099000940172573 1102 48-49 0.014891462470146166 1102 50-51 0.027827610507571876 1102 52-53 0.018295092522294 1102 54-55 0.03924419660473433 1102 56-57 0.024012246016205552 1102 58-59 0.0044783501945673265 1102 60-61 0.004513857197331106 1102 62-63 0.008037029023746811 1102 64-65 -0.033529569995451425 1102 66-67 -0.023815288603550755 1102 68-69 9.453825220333556E-4 1102 70-71 -0.035848648729057686 1102 72-73 0.03708774541087223 1102 74-75 -0.05307777662319069 1102 76-77 -0.027115616734874948 1102 78-79 0.003780646689023115 1102 80-81 0.05463922455310666 1102 82-83 0.03088965994881221 1102 84-85 -0.02563390260258558 1102 86-87 -0.022212974481440995 1102 88-89 -0.07853142814377634 1102 90-91 -0.05660244757319788 1102 92-93 -0.018907619268244247 1102 94-95 -0.06212038010812648 1102 96-97 -0.09694034737045953 1102 98-99 -0.10536773010304756 1102 100 -0.05384924589117546 1103 1 0.2406126011502039 1103 2 0.23920045709672877 1103 3 0.3625409136991351 1103 4 0.19377836959399986 1103 5 0.22555549077920034 1103 6 0.2208193072542599 1103 7 0.1973494200729391 1103 8 0.1955287433690316 1103 9 0.2582595856378518 1103 10-11 0.24660434235677542 1103 12-13 0.21727790963446125 1103 14-15 0.32331065130783543 1103 16-17 0.33126169984522136 1103 18-19 0.33460794729521837 1103 20-21 0.3365627275546501 1103 22-23 0.30900215798784814 1103 24-25 0.3095460853595924 1103 26-27 0.307509565173973 1103 28-29 0.3123951270385703 1103 30-31 0.3116869588140645 1103 32-33 0.28465004846998454 1103 34-35 0.28322884403629445 1103 36-37 0.313777093659823 1103 38-39 0.35506544096877946 1103 40-41 0.31611019076855484 1103 42-43 0.2981509081874236 1103 44-45 0.23985630931617408 1103 46-47 0.2575119776865904 1103 48-49 0.23509322511718267 1103 50-51 0.2103801792543294 1103 52-53 0.17103867765676029 1103 54-55 0.21772767797592962 1103 56-57 0.1699356199769113 1103 58-59 0.1750934937580766 1103 60-61 0.10339743482895614 1103 62-63 0.1754826366693507 1103 64-65 0.1337091721581487 1103 66-67 0.1310497465204321 1103 68-69 0.13367161733236088 1103 70-71 0.08154556411722069 1103 72-73 0.08274188486996792 1103 74-75 0.09877081607273652 1103 76-77 0.12481446842760135 1103 78-79 0.15202969027194513 1103 80-81 0.09506661142756201 1103 82-83 0.037465393722939666 1103 84-85 0.08052802691758387 1103 86-87 0.07513535346573263 1103 88-89 0.15981299174710273 1103 90-91 0.09696538379259323 1103 92-93 0.08920813487818435 1103 94-95 0.09713918308574421 1103 96-97 0.12449796779844036 1103 98-99 0.22390652941455258 1103 100 0.20621771958662904 1104 1 0.1877199624868453 1104 2 0.20852315301278423 1104 3 0.29086214167902114 1104 4 0.16822235304528022 1104 5 0.14384972807651764 1104 6 0.11967460360239102 1104 7 0.1465487131025469 1104 8 0.12176497012751497 1104 9 0.24141964939507687 1104 10-11 0.2129738105458756 1104 12-13 0.17525401220045467 1104 14-15 0.27077322851349095 1104 16-17 0.24010707627456185 1104 18-19 0.21121099846257607 1104 20-21 0.2730104493334409 1104 22-23 0.243484098230212 1104 24-25 0.2663653929369545 1104 26-27 0.28458919138638095 1104 28-29 0.29573438047415834 1104 30-31 0.3377722025240999 1104 32-33 0.3177160972656452 1104 34-35 0.34136281729354323 1104 36-37 0.29177377116060654 1104 38-39 0.2785072470839296 1104 40-41 0.3222443394679715 1104 42-43 0.3214046259765979 1104 44-45 0.28950444577076695 1104 46-47 0.24750850388505796 1104 48-49 0.2567594171633658 1104 50-51 0.22778247654211725 1104 52-53 0.2817674058396946 1104 54-55 0.2503854227117088 1104 56-57 0.26263658554734803 1104 58-59 0.2510720490880445 1104 60-61 0.19202158943796377 1104 62-63 0.27115031129134337 1104 64-65 0.18645448963175681 1104 66-67 0.26874674030769796 1104 68-69 0.20396667982301864 1104 70-71 0.23257226870369152 1104 72-73 0.24323247932453995 1104 74-75 0.22463966554203552 1104 76-77 0.22144882702686175 1104 78-79 0.23518775665169045 1104 80-81 0.21496851955769358 1104 82-83 0.1823896428900582 1104 84-85 0.17228185399795848 1104 86-87 0.16718664573002684 1104 88-89 0.17401406333816993 1104 90-91 0.17478998508217103 1104 92-93 0.1937316088915253 1104 94-95 0.1571170998000042 1104 96-97 0.20577183168974145 1104 98-99 0.16990276679145921 1104 100 0.17490686138322076 1105 1 0.17213035970377888 1105 2 0.17206314227311026 1105 3 0.28519935158131204 1105 4 0.1695245529238889 1105 5 0.16309151301713598 1105 6 0.10068702846772482 1105 7 0.13694547277992086 1105 8 0.1284433722943774 1105 9 0.19996302361990814 1105 10-11 0.1913416982572187 1105 12-13 0.19050097452543469 1105 14-15 0.2196163228016914 1105 16-17 0.26206896277676606 1105 18-19 0.24291614363317393 1105 20-21 0.23322201759196304 1105 22-23 0.19568680212766765 1105 24-25 0.20871732997886028 1105 26-27 0.18627557373994819 1105 28-29 0.25947213042644535 1105 30-31 0.24857221881315894 1105 32-33 0.2570664234688351 1105 34-35 0.2685862501929819 1105 36-37 0.30846152632264534 1105 38-39 0.30491531312237186 1105 40-41 0.37410719893201616 1105 42-43 0.31520010373800744 1105 44-45 0.29255111206450835 1105 46-47 0.2564931166683948 1105 48-49 0.29125388485756076 1105 50-51 0.23889776018882003 1105 52-53 0.2233960484901587 1105 54-55 0.23993330287851222 1105 56-57 0.21046737164429885 1105 58-59 0.23978016844729666 1105 60-61 0.21398562818063027 1105 62-63 0.26558484399718196 1105 64-65 0.24859039874634448 1105 66-67 0.2138633435043502 1105 68-69 0.2851092888055362 1105 70-71 0.28182611242108635 1105 72-73 0.339010795460311 1105 74-75 0.27732442599361207 1105 76-77 0.27188577998374086 1105 78-79 0.21067094360404326 1105 80-81 0.20121102091119525 1105 82-83 0.22210861767823786 1105 84-85 0.19266899901302992 1105 86-87 0.22691013871284582 1105 88-89 0.21651479004971463 1105 90-91 0.25066207811092056 1105 92-93 0.23707647640875962 1105 94-95 0.22893372045435 1105 96-97 0.20152338452652074 1105 98-99 0.19773976946508753 1105 100 0.16697038026730837 1106 1 0.08496692217864066 1106 2 0.08184199872890474 1106 3 0.17908515095822963 1106 4 0.14145646587702032 1106 5 0.13640146053089808 1106 6 0.12023841368813493 1106 7 0.09695884157132895 1106 8 0.09027529846409976 1106 9 0.09808192852904085 1106 10-11 0.13639951263164107 1106 12-13 0.17519765136331955 1106 14-15 0.20180829459980032 1106 16-17 0.1697442523998589 1106 18-19 0.18443241245594777 1106 20-21 0.19060295606157496 1106 22-23 0.1808806313501492 1106 24-25 0.1700047278097614 1106 26-27 0.1689663597588762 1106 28-29 0.22328850700340297 1106 30-31 0.19107586354693495 1106 32-33 0.21434648938725331 1106 34-35 0.21652836751074744 1106 36-37 0.198309908194517 1106 38-39 0.16141703488460024 1106 40-41 0.20893409925491824 1106 42-43 0.15873102116587745 1106 44-45 0.14581273447036125 1106 46-47 0.15703259048235907 1106 48-49 0.21600424209331948 1106 50-51 0.20141360915729223 1106 52-53 0.1833104971609174 1106 54-55 0.20343591932614657 1106 56-57 0.2135618650872786 1106 58-59 0.2142635721247359 1106 60-61 0.19822272103007776 1106 62-63 0.2224409872980928 1106 64-65 0.23354169977069006 1106 66-67 0.15061188590597396 1106 68-69 0.21077507261640704 1106 70-71 0.2270684087794521 1106 72-73 0.2644154711991078 1106 74-75 0.2438930758036193 1106 76-77 0.19749344493183685 1106 78-79 0.15799792724577344 1106 80-81 0.18224162345288164 1106 82-83 0.2039993249025258 1106 84-85 0.21344664683259396 1106 86-87 0.26044084491496733 1106 88-89 0.21871556976147843 1106 90-91 0.23009584673576455 1106 92-93 0.16594300220861413 1106 94-95 0.13796592206389136 1106 96-97 0.2203597987619581 1106 98-99 0.16444310065849876 1106 100 0.13978686231184057 1107 1 0.09414562373775937 1107 2 0.0751524982438383 1107 3 0.17196931393227288 1107 4 0.10121632233880717 1107 5 0.15980611862015337 1107 6 0.11363467602979682 1107 7 0.0886834564763106 1107 8 0.08795512812491779 1107 9 0.07734659077579664 1107 10-11 0.09357692909977544 1107 12-13 0.11434114650241156 1107 14-15 0.12998138144857307 1107 16-17 0.16093257242459202 1107 18-19 0.12281821467814069 1107 20-21 0.19026009533871502 1107 22-23 0.1749232768550968 1107 24-25 0.15720535360464538 1107 26-27 0.10863274825366886 1107 28-29 0.179185224581083 1107 30-31 0.17288617440097198 1107 32-33 0.1259917107847599 1107 34-35 0.13570805248360784 1107 36-37 0.15239932579977733 1107 38-39 0.14446767546014883 1107 40-41 0.2169790075817275 1107 42-43 0.12712084436520144 1107 44-45 0.19306479474467864 1107 46-47 0.14670251864291828 1107 48-49 0.09381385395433028 1107 50-51 0.13022555976192507 1107 52-53 0.19154825923003216 1107 54-55 0.2488217891537019 1107 56-57 0.16127399354842709 1107 58-59 0.17301626979877938 1107 60-61 0.21303466290249418 1107 62-63 0.19126284723427034 1107 64-65 0.15640093053808357 1107 66-67 0.16942274047900696 1107 68-69 0.154751155772324 1107 70-71 0.17547654156262382 1107 72-73 0.173274310333575 1107 74-75 0.15985543330348406 1107 76-77 0.17679734150610216 1107 78-79 0.17071515972128282 1107 80-81 0.15677196975416763 1107 82-83 0.19330917047156504 1107 84-85 0.18830450317705782 1107 86-87 0.1420871218016586 1107 88-89 0.14792615291509037 1107 90-91 0.16368372125120345 1107 92-93 0.1701944127992121 1107 94-95 0.16180470465172903 1107 96-97 0.1737656779752541 1107 98-99 0.232775854327528 1107 100 0.28815142411386674 1108 1 -0.016674309233511764 1108 2 0.006036400857226454 1108 3 0.062288119577921464 1108 4 0.019238002668984677 1108 5 0.012052377477139942 1108 6 0.060481737109540745 1108 7 0.01733377668431757 1108 8 -0.005970366470066324 1108 9 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-0.2459733693163848 2214 96-97 -0.25384724855890894 2214 98-99 -0.24566935749655983 2214 100 -0.23669818997440473 2215 1 -0.32955658530501 2215 2 -0.3286532022870965 2215 3 -0.2467050602610712 2215 4 -0.22518622721698023 2215 5 -0.23329169567784191 2215 6 -0.2362234400237 2215 7 -0.27714463399395584 2215 8 -0.3002055786830766 2215 9 -0.43761820850278355 2215 10-11 -0.36314044735308926 2215 12-13 -0.4018593884465105 2215 14-15 -0.5504928490637511 2215 16-17 -0.577215082723491 2215 18-19 -0.5397239865110848 2215 20-21 -0.48593572071632707 2215 22-23 -0.50240268468648 2215 24-25 -0.5273453923343681 2215 26-27 -0.5213637013001886 2215 28-29 -0.43147083556818444 2215 30-31 -0.43274843578216604 2215 32-33 -0.4157214295164877 2215 34-35 -0.4551450947775919 2215 36-37 -0.4756375776981372 2215 38-39 -0.4589075246664791 2215 40-41 -0.5094644517395892 2215 42-43 -0.478974823456241 2215 44-45 -0.4868266425066423 2215 46-47 -0.48199307788770795 2215 48-49 -0.4293975929433813 2215 50-51 -0.424262639691122 2215 52-53 -0.5178342140256973 2215 54-55 -0.4830496948439915 2215 56-57 -0.36206370631216345 2215 58-59 -0.3511197800839341 2215 60-61 -0.3636263123383898 2215 62-63 -0.431606388241498 2215 64-65 -0.5022178796664036 2215 66-67 -0.4569195543833615 2215 68-69 -0.4374757068834825 2215 70-71 -0.3310967310339805 2215 72-73 -0.343094951851576 2215 74-75 -0.305316790114432 2215 76-77 -0.3233655293967814 2215 78-79 -0.29668290026081223 2215 80-81 -0.4115846613558958 2215 82-83 -0.41934331442493544 2215 84-85 -0.38255880774717355 2215 86-87 -0.34996716577433773 2215 88-89 -0.3389575772323994 2215 90-91 -0.37753159547777315 2215 92-93 -0.2896954120224251 2215 94-95 -0.34606383243337646 2215 96-97 -0.27924416728928136 2215 98-99 -0.27842035567339707 2215 100 -0.29374397853054646 2216 1 -0.26175427591073586 2216 2 -0.22571372357086972 2216 3 -0.20959951453161096 2216 4 -0.20616768615697367 2216 5 -0.283692847012432 2216 6 -0.25979066061321987 2216 7 -0.26268766221017614 2216 8 -0.25621449838779853 2216 9 -0.38673834318382205 2216 10-11 -0.39693422485588314 2216 12-13 -0.444811218902629 2216 14-15 -0.5902667124683063 2216 16-17 -0.6268251398642946 2216 18-19 -0.5805083780365763 2216 20-21 -0.5033274356354838 2216 22-23 -0.5113043998073863 2216 24-25 -0.47689030021190604 2216 26-27 -0.5282910154580591 2216 28-29 -0.5507704291488693 2216 30-31 -0.5292844004171897 2216 32-33 -0.5101506210939917 2216 34-35 -0.4972900270896119 2216 36-37 -0.5879653874947905 2216 38-39 -0.5942211434124332 2216 40-41 -0.6038410195250847 2216 42-43 -0.6313772200524568 2216 44-45 -0.6775497797140773 2216 46-47 -0.6074616762230249 2216 48-49 -0.5942741820249822 2216 50-51 -0.5841577900079855 2216 52-53 -0.5777083764723727 2216 54-55 -0.5930369788286498 2216 56-57 -0.5653639578303498 2216 58-59 -0.5472642326732 2216 60-61 -0.6419353344885863 2216 62-63 -0.6145721676452496 2216 64-65 -0.6458290486879719 2216 66-67 -0.6263812785607143 2216 68-69 -0.6309007311498291 2216 70-71 -0.6386873907872612 2216 72-73 -0.6729358643559777 2216 74-75 -0.6888911336900705 2216 76-77 -0.5625093643990553 2216 78-79 -0.5813647252696157 2216 80-81 -0.5750311610611121 2216 82-83 -0.6081167653575932 2216 84-85 -0.6694503836812302 2216 86-87 -0.652765641870019 2216 88-89 -0.6292649360448372 2216 90-91 -0.5645042927525452 2216 92-93 -0.5893567199170064 2216 94-95 -0.6387716263588317 2216 96-97 -0.6153590799416335 2216 98-99 -0.7055728032681721 2216 100 -0.7500496426215051 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 376.0 3 35.0 4 53.0 5 130.0 6 436.0 7 1130.0 8 2277.0 9 3734.0 10 5082.0 11 6660.0 12 9918.0 13 14079.0 14 15750.0 15 19866.0 16 25122.0 17 36421.0 18 36706.0 19 19005.0 20 13593.0 21 12689.0 22 13125.0 23 14083.0 24 16341.0 25 19925.0 26 24609.0 27 31943.0 28 41255.0 29 53808.0 30 69970.0 31 91455.0 32 118718.0 33 156931.0 34 217250.0 35 323504.0 36 536133.0 37 1037113.0 38 1511454.0 39 630371.0 40 1875.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.02181903867956 20.766982435689467 14.625169607836252 22.58602891779472 2 15.74646035155394 21.37839535937112 39.46079477101263 23.414349518062313 3 17.82578159626334 26.289435360929687 31.28993702421134 24.594846018595636 4 12.755027591480491 18.257250982626864 38.29202647613203 30.695694949760615 5 12.250692433068052 37.716213017059715 35.429756647406165 14.603337902466066 6 29.072750697313595 38.731729916326266 18.01113714571044 14.184382240649704 7 25.404618224501625 33.49581379038268 23.13265827963588 17.966909705479818 8 24.593832950997726 35.51361066058826 21.025516640122348 18.867039748291667 9 24.621127329933714 16.703770267440106 22.371805549467407 36.30329685315877 10-11 23.11074095179649 27.25114822445292 29.209057213966695 20.429053609783896 12-13 24.276187553880096 25.066497172664707 29.388915676734022 21.268399596721167 14-15 21.641880604138965 26.970655133281706 27.509071338466857 23.87839292411247 16-17 20.174705455466423 30.09084488863562 28.17651728790115 21.557932367996806 18-19 19.989538128844664 28.040327883224474 31.52614737211239 20.443986615818467 20-21 21.195137275530033 27.3891689436335 31.334658114038294 20.08103566679817 22-23 20.825007184013014 27.358932772249737 31.413229299083856 20.40283074465339 24-25 20.610460897051876 27.36260514229216 31.46242152379004 20.56451243686592 26-27 20.4133413209817 27.322384410448237 31.48052036606808 20.783753902501985 28-29 20.500026787845137 27.29107672525899 31.613397818982353 20.59549866791352 30-31 20.865890306209423 27.64548478693922 31.132706984808856 20.3559179220425 32-33 20.412308771314603 27.48391024610724 31.49759639971361 20.606184582864547 34-35 20.634404360087085 27.14349031010584 31.402991471724214 20.819113858082865 36-37 21.108091390386573 27.441166586303133 30.840368795569777 20.61037322774052 38-39 20.894528947919557 27.519182532376764 30.98632845794552 20.59996006175816 40-41 20.7265058421855 27.315838435200202 31.039904148219584 20.917751574394718 42-43 20.707140665410073 27.43743577005314 30.82915686475061 21.026266699786184 44-45 20.960456270060444 27.65579080154103 30.464842170886968 20.918910757511554 46-47 21.055392393226086 27.880594397931002 29.9909116147226 21.07310159412031 48-49 21.060360320869677 27.73587184694886 29.880117087235835 21.32365074494562 50-51 21.12286853986762 27.746265530862036 29.64914741594705 21.4817185133233 52-53 21.15728361509276 27.909008995845447 29.45523264025872 21.47847474880307 54-55 21.238007568783882 28.265891280312882 29.314221423457386 21.181879727445853 56-57 21.003609053317554 27.985076735000025 29.352474466313065 21.658839745369356 58-59 20.903003648017457 27.558244081103854 29.71250310495478 21.826249165923915 60-61 21.183574667465432 27.935942956501407 29.342587316198852 21.537895059834305 62-63 20.994549891143937 27.88668254455306 29.479439111228 21.639328453075002 64-65 20.958059975550004 27.63731205891378 29.657183769488153 21.747444196048065 66-67 20.920742072015468 27.72550738613948 29.4567230185518 21.89702752329325 68-69 21.267230672569735 27.88787095077368 29.263548561492712 21.58134981516387 70-71 21.283089076890857 27.59714977327742 29.308815149257 21.81094600057472 72-73 21.029861141551844 27.710876352177365 29.467175148672542 21.79208735759825 74-75 21.060555141561586 27.759152919631596 29.260372984214655 21.91991895459217 76-77 21.174720067018317 27.79306146105778 29.03257889020393 21.999639581719972 78-79 21.21053785122518 27.841036056439556 29.145302142540558 21.80312394979471 80-81 20.995879732370607 27.68873812347321 29.20700677333891 22.108375370817267 82-83 20.8962721614746 27.724340303929907 29.319317718230693 22.060069816364802 84-85 20.90015826309781 27.66466614396107 29.1671573728618 22.268018220079316 86-87 21.028977169449725 27.744601836426675 29.047889663997157 22.178531330126443 88-89 20.82367830128064 27.615206758282156 29.10021465440018 22.460900286037024 90-91 20.686788266731504 27.53525692728388 29.107908368225942 22.670046437758675 92-93 20.755658555321457 27.695683942462697 28.945899828625592 22.60275767359025 94-95 20.63966406599917 27.44249707719574 29.16267039788128 22.755168458923812 96-97 20.594218041001017 27.53396376354328 29.084598814275836 22.787219381179867 98-99 20.563971253498046 27.484731732824613 29.03543399128908 22.915863022388265 100 20.65831119194876 27.441562525204944 28.968587482919084 22.931538799927214 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1703.0 1 1270.0 2 2188.0 3 3245.0 4 1909.5 5 1003.5 6 2057.5 7 4868.5 8 7812.5 9 8919.0 10 6951.0 11 4382.5 12 3891.5 13 4258.0 14 4790.0 15 5310.0 16 5882.0 17 6655.0 18 7531.5 19 8338.0 20 9537.0 21 11349.5 22 13900.5 23 18028.0 24 23962.0 25 31165.0 26 40721.0 27 52669.5 28 67834.0 29 84616.5 30 99038.5 31 112650.0 32 125529.5 33 136603.5 34 146267.5 35 154468.0 36 160968.0 37 167805.5 38 175098.5 39 178886.0 40 180994.5 41 181500.5 42 179915.0 43 179019.0 44 177041.0 45 173847.0 46 170909.5 47 165724.0 48 161706.0 49 159670.5 50 155636.0 51 148101.0 52 136260.5 53 126509.0 54 120034.0 55 113335.0 56 104511.5 57 94174.5 58 85172.0 59 77468.5 60 68011.5 61 56917.0 62 47038.5 63 38230.5 64 30534.5 65 24322.0 66 19285.5 67 14770.0 68 10991.5 69 8287.0 70 6048.5 71 4267.0 72 3112.0 73 2156.5 74 1513.0 75 1024.5 76 611.5 77 416.5 78 291.5 79 168.0 80 80.5 81 36.0 82 24.5 83 15.5 84 6.0 85 3.5 86 5.5 87 3.0 88 1.5 89 1.0 90 0.5 91 0.5 92 0.5 93 0.0 94 0.5 95 0.5 96 0.5 97 0.5 98 0.0 99 2.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12454886833530589 2 0.0 3 0.0 4 0.0 5 0.012858165665775363 6 2.3378483028682476E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.1689241514341238E-4 82-83 6.039441449076306E-4 84-85 3.2145414164438404E-4 86-87 4.2860552219251203E-4 88-89 4.480875913830808E-4 90-91 6.429082832887681E-4 92-93 2.240437956915404E-4 94-95 2.3378483028682476E-4 96-97 5.454979373359244E-4 98-99 0.0011104779438624176 100 5.844620757170619E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5132925.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.07486899900275 #Duplication Level Percentage of deduplicated Percentage of total 1 76.89104796560964 46.961106816956466 2 14.277789515390207 17.440282484955837 3 4.099881294223287 7.511991388684475 4 1.7105832772710827 4.1789459828486475 5 0.8375622122786518 2.5577001196716798 6 0.510319341487623 1.8700612157408356 7 0.328259233142489 1.4033872763323434 8 0.22372921186567146 1.0931385844756816 9 0.16521508301072618 0.908144059638552 >10 0.8795144435141024 9.58483664034328 >50 0.05062791680461255 2.107377042674352 >100 0.024225944064056548 2.5614491701663473 >500 7.339739620157667E-4 0.2881811696574265 >1k 3.8294053201217153E-4 0.4696708800333697 >5k 3.1911711000263486E-5 0.10770193721603956 >10k+ 9.573513300079047E-5 0.9560252306046277 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19078 0.3716789160176702 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18967 0.3695164063375171 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11007 0.21443913558059002 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5526 0.10765791434708281 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 1.948206919056873E-5 0.0 0.0 0.0 4 0.0 1.948206919056873E-5 0.0 0.0 0.0 5 0.0 5.844620757170619E-5 0.0 0.0 0.0 6 0.0 5.844620757170619E-5 0.0 0.0 0.0 7 0.0 5.844620757170619E-5 0.0 0.0 0.0 8 0.0 5.844620757170619E-5 0.0 0.0 0.0 9 0.0 5.844620757170619E-5 0.0 0.0 0.0 10-11 0.0 6.818724216699055E-5 0.0 9.741034595284366E-6 0.0 12-13 0.0 9.741034595284366E-5 0.0 1.948206919056873E-5 0.0 14-15 0.0 1.2663344973869674E-4 0.0 1.948206919056873E-5 0.0 16-17 1.948206919056873E-5 1.363744843339811E-4 0.0 5.844620757170619E-5 0.0 18-19 4.870517297642183E-5 1.5585655352454985E-4 0.0 7.792827676227493E-5 0.0 20-21 5.844620757170619E-5 1.655975881198342E-4 0.0 1.5585655352454985E-4 0.0 22-23 5.844620757170619E-5 1.7533862271511857E-4 0.0 3.8964138381137464E-4 0.0 24-25 5.844620757170619E-5 1.7533862271511857E-4 0.0 9.838444941237209E-4 0.0 26-27 5.844620757170619E-5 1.7533862271511857E-4 0.0 0.0019287248498663044 0.0 28-29 5.844620757170619E-5 1.7533862271511857E-4 0.0 0.004938704539809173 0.0 30-31 6.818724216699055E-5 1.7533862271511857E-4 0.0 0.013033504288490481 0.0 32-33 7.792827676227493E-5 1.7533862271511857E-4 0.0 0.03172654967684118 0.0 34-35 7.792827676227493E-5 1.948206919056873E-4 0.0 0.06234262140981994 0.0 36-37 7.792827676227493E-5 1.948206919056873E-4 0.0 0.10873916918715937 0.0 38-39 1.1689241514341238E-4 2.0456172650097165E-4 0.0 0.16922125298927998 0.0 40-41 1.1689241514341238E-4 2.1430276109625604E-4 0.0 0.23606423238212132 0.0 42-43 1.1689241514341238E-4 2.1430276109625604E-4 0.0 0.30691077699362446 0.0 44-45 1.1689241514341238E-4 2.1430276109625604E-4 0.0 0.3948041321468753 0.0 46-47 1.1689241514341238E-4 2.1430276109625604E-4 0.0 0.5039913889254177 0.0 48-49 1.363744843339811E-4 2.1430276109625604E-4 0.0 0.6163347409128324 0.0 50-51 1.363744843339811E-4 2.532668994773935E-4 0.0 0.7321653832853587 0.0 52-53 1.363744843339811E-4 2.92231037858531E-4 0.0 0.846827101506451 0.0 54-55 1.363744843339811E-4 3.311951762396684E-4 0.0 0.9809221837451356 0.0 56-57 1.363744843339811E-4 3.7015931462080587E-4 0.0 1.143996843904791 0.0 58-59 1.363744843339811E-4 3.7015931462080587E-4 0.0 1.3205725780135107 0.0 60-61 1.5585655352454982E-4 3.7015931462080587E-4 0.0 1.506373071883965 0.0 62-63 1.7533862271511857E-4 3.7015931462080587E-4 0.0 1.6930210357642086 0.0 64-65 1.7533862271511857E-4 3.896413838113746E-4 0.0 1.9005245547129561 0.0 66-67 2.1430276109625604E-4 4.0912345300194336E-4 0.0 2.141887909914912 0.0 68-69 2.240437956915404E-4 4.0912345300194336E-4 0.0 2.4081396085078195 0.0 70-71 2.3378483028682476E-4 4.0912345300194336E-4 0.0 2.6943701690556554 0.0 72-73 2.3378483028682476E-4 4.0912345300194336E-4 0.0 2.9929133973319306 0.0 74-75 2.3378483028682476E-4 4.0912345300194336E-4 0.0 3.3005255288164155 0.0 76-77 2.3378483028682476E-4 4.0912345300194336E-4 0.0 3.6303764422819347 0.0 78-79 2.3378483028682476E-4 4.0912345300194336E-4 0.0 4.00051627483355 0.0 80-81 2.3378483028682476E-4 4.0912345300194336E-4 0.0 4.403376242590726 0.0 82-83 2.4352586488210912E-4 4.0912345300194336E-4 0.0 4.817603997720598 0.0 84-85 2.532668994773935E-4 4.286055221925121E-4 0.0 5.234169601153338 0.0 86-87 2.532668994773935E-4 4.286055221925121E-4 0.0 5.671727134138917 0.0 88 2.532668994773935E-4 4.286055221925121E-4 0.0 6.0281418489457765 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11130 0.0 59.208405 1 GTATCAA 19820 0.0 39.874706 1 GTGGTAT 4465 0.0 37.846306 1 TGGTATC 4510 0.0 36.688343 2 ATCAACG 24775 0.0 31.60991 3 TCAACGC 25140 0.0 31.300562 4 TATCAAC 25550 0.0 30.761484 2 CAACGCA 26085 0.0 30.148592 5 AACGCAG 27140 0.0 29.075748 6 ACGCAGA 30435 0.0 25.804113 7 CGCAGAG 30710 0.0 25.4047 8 GCGGTAT 335 0.0 25.29171 1 GCAGAGT 33180 0.0 23.201889 9 GTACATG 15360 0.0 22.615993 1 TACCTGG 3825 0.0 21.998018 2 GAGTACT 24840 0.0 21.54107 12-13 CAGAGTA 32545 0.0 21.38011 10-11 TACATGG 15665 0.0 21.335419 2 GTACCTG 4350 0.0 20.992441 1 ACATGGG 15600 0.0 20.791529 3 >>END_MODULE