FastQCFastQC Report
Wed 25 May 2016
SRR2049446_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049446_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8261136
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86700.042.25191
GTATCAA140000.033.387681
TCAACGC145000.031.0511824
ATCAACG146150.030.8072263
CAACGCA159050.028.3686895
TATCAAC168650.026.864532
AACGCAG174950.025.8979136
GTACATG222550.022.9683531
CGCAGAG193900.022.8081368
ACGCAGA198400.022.6242357
TACATGG223700.021.6191182
GTGGTAT50700.021.2400441
ACATGGG227600.020.2780023
GCAGAGT225550.018.816049
TGGTATC56950.017.6607282
AGAGTAC193700.016.7663910-11
CAGAGTA213900.016.75406610-11
CATGGGG152200.016.520374
TACCTGG56700.015.4176592
GAGTACA171150.015.3315191
Produced by FastQC (version 0.11.5)