FastQCFastQC Report
Wed 25 May 2016
SRR2049446_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049446_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8261136
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236790.2866312817026617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230840.2794288824200449No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127720.15460343468501184No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA143550.057.2217861
GTATCAA262550.040.196921
ATCAACG323250.032.1964843
TCAACGC326050.032.0064934
GTGGTAT60500.031.6670951
TATCAAC338800.030.8991242
CAACGCA338900.030.792915
AACGCAG356550.029.368386
TGGTATC65250.028.0245342
ACGCAGA403950.025.8059087
CGCAGAG404950.025.5564868
GCAGAGT445100.022.8710489
GTACATG216250.022.0289361
GAGTACT318400.021.40327312-13
TACATGG219450.020.949452
CAGAGTA430950.020.71015210-11
AGAGTAC409050.020.62976310-11
ACATGGG222250.019.6914313
GTACTTT355050.019.14097214-15
AGTACTT334600.018.87108812-13
Produced by FastQC (version 0.11.5)