##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049446_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8261136
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.947034887211636	33.0	31.0	34.0	30.0	34.0
2	32.12386698391117	33.0	31.0	34.0	30.0	34.0
3	32.145324686580636	34.0	31.0	34.0	30.0	34.0
4	35.817582715016435	37.0	35.0	37.0	35.0	37.0
5	35.87741334847895	37.0	35.0	37.0	35.0	37.0
6	35.94689846529582	37.0	35.0	37.0	35.0	37.0
7	35.911240173264304	37.0	35.0	37.0	35.0	37.0
8	35.9295587192851	37.0	35.0	37.0	35.0	37.0
9	37.6605907468416	39.0	37.0	39.0	35.0	39.0
10-11	37.63432910437499	39.0	37.0	39.0	35.0	39.0
12-13	37.614193859052804	39.0	37.0	39.0	35.0	39.0
14-15	38.91359620517082	40.0	38.0	41.0	35.0	41.0
16-17	38.92162863557748	40.0	38.0	41.0	35.5	41.0
18-19	38.99685000949022	40.0	38.5	41.0	36.0	41.0
20-21	39.01952558340645	40.0	39.0	41.0	35.0	41.0
22-23	38.98927187495764	40.0	39.0	41.0	35.0	41.0
24-25	38.96981468408219	40.0	38.5	41.0	35.0	41.0
26-27	38.872555663046825	40.0	38.0	41.0	35.0	41.0
28-29	38.79307422126932	40.0	38.0	41.0	35.0	41.0
30-31	38.68624200110009	40.0	38.0	41.0	35.0	41.0
32-33	38.55661061626391	40.0	38.0	41.0	34.5	41.0
34-35	38.44288812095576	40.0	38.0	41.0	34.0	41.0
36-37	38.31532279579952	40.0	38.0	41.0	34.0	41.0
38-39	38.21368810536468	40.0	38.0	41.0	34.0	41.0
40-41	38.12639018411027	40.0	38.0	41.0	34.0	41.0
42-43	37.99179126212182	40.0	38.0	41.0	33.0	41.0
44-45	37.82357420335411	40.0	38.0	41.0	33.0	41.0
46-47	37.58689682629604	40.0	37.0	41.0	33.0	41.0
48-49	37.34457331291968	40.0	37.0	41.0	32.5	41.0
50-51	37.1901903684917	40.0	37.0	41.0	32.0	41.0
52-53	37.07770063342378	40.0	37.0	41.0	32.0	41.0
54-55	36.938808778841064	40.0	36.5	41.0	31.5	41.0
56-57	36.79523893566211	40.0	36.0	41.0	31.0	41.0
58-59	36.621392263727415	39.0	36.0	41.0	31.0	41.0
60-61	36.4366814079807	39.0	35.0	41.0	31.0	41.0
62-63	36.21877584390331	39.0	35.0	41.0	31.0	41.0
64-65	35.997937269160076	39.0	35.0	41.0	30.5	41.0
66-67	35.7335607354727	38.0	35.0	40.0	30.0	41.0
68-69	35.43734057882597	37.0	35.0	40.0	30.0	41.0
70-71	35.14621778409168	37.0	35.0	40.0	29.5	41.0
72-73	34.808373751503424	36.5	34.5	39.0	29.0	41.0
74-75	34.48131310270161	36.0	34.0	39.0	29.0	41.0
76-77	34.15321222166055	35.5	34.0	38.5	29.0	40.0
78-79	33.83675507823621	35.0	34.0	37.0	29.0	39.0
80-81	33.52051733563036	35.0	34.0	37.0	29.0	39.0
82-83	33.23979135557144	35.0	34.0	36.5	29.0	39.0
84-85	32.950372987443856	35.0	34.0	36.0	28.5	37.5
86-87	32.70154921792838	35.0	34.0	36.0	28.0	37.0
88-89	32.47659492592786	35.0	34.0	35.5	27.5	37.0
90-91	32.28302747951371	35.0	33.0	35.0	27.0	36.0
92-93	32.11772781612601	35.0	33.0	35.0	27.0	36.0
94-95	31.956173702987094	35.0	33.0	35.0	27.0	36.0
96-97	31.80978281921518	35.0	33.0	35.0	26.5	36.0
98-99	31.672553266282023	35.0	33.0	35.0	26.0	35.0
100	31.563230529070093	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.30858192938487505
1101	2	0.3350066379894443
1101	3	0.4445643161053283
1101	4	0.2664652399412688
1101	5	0.26719332520334405
1101	6	0.24315583012798925
1101	7	0.26341823120531416
1101	8	0.2859492152182952
1101	9	0.34988143775699854
1101	10-11	0.31349373856608764
1101	12-13	0.33106990170006156
1101	14-15	0.44591762893054465
1101	16-17	0.429738170632902
1101	18-19	0.4356849485415921
1101	20-21	0.4492613593621826
1101	22-23	0.4603840200469449
1101	24-25	0.41653961265126327
1101	26-27	0.39008874920882164
1101	28-29	0.40818346948056927
1101	30-31	0.4335944985803408
1101	32-33	0.44510546005961515
1101	34-35	0.45720633920665676
1101	36-37	0.4799460620425364
1101	38-39	0.4384223622459942
1101	40-41	0.42224198393876833
1101	42-43	0.453420414540588
1101	44-45	0.4891768960097096
1101	46-47	0.5405235944917663
1101	48-49	0.5281933447313207
1101	50-51	0.46118014663790063
1101	52-53	0.4795776712220672
1101	54-55	0.4553816427608197
1101	56-57	0.5073918796897345
1101	58-59	0.4558447274923765
1101	60-61	0.43601662990045753
1101	62-63	0.47354310824565715
1101	64-65	0.42670207596239607
1101	66-67	0.4517098587241932
1101	68-69	0.4522313219381857
1101	70-71	0.4303210681846821
1101	72-73	0.4665877404747363
1101	74-75	0.37109859057247974
1101	76-77	0.41090012926783004
1101	78-79	0.4323748935697438
1101	80-81	0.40458530208359633
1101	82-83	0.386298378752727
1101	84-85	0.40279998695041996
1101	86-87	0.37858524930133797
1101	88-89	0.4020576748050928
1101	90-91	0.4279143621279289
1101	92-93	0.4300798426273005
1101	94-95	0.41440236562309707
1101	96-97	0.4201143880143974
1101	98-99	0.5245357244968716
1101	100	0.4948592391183624
1102	1	0.2713574603991411
1102	2	0.2930753088175564
1102	3	0.352486021236885
1102	4	0.2402748903954972
1102	5	0.19622974427183237
1102	6	0.1726288742710267
1102	7	0.20336661241871923
1102	8	0.19924985507122273
1102	9	0.20624822711147317
1102	10-11	0.25336210675124704
1102	12-13	0.26692508862444697
1102	14-15	0.3544687582503059
1102	16-17	0.38111305796897454
1102	18-19	0.3369420657746218
1102	20-21	0.36715820234301333
1102	22-23	0.3483679793282164
1102	24-25	0.33452517673037363
1102	26-27	0.2975198182903753
1102	28-29	0.34487089660501624
1102	30-31	0.33982957793930524
1102	32-33	0.3364371051093258
1102	34-35	0.3988809949604146
1102	36-37	0.4157518450666018
1102	38-39	0.40970009334805724
1102	40-41	0.41388632025190475
1102	42-43	0.3821849812744915
1102	44-45	0.4598022722541728
1102	46-47	0.43849588461016964
1102	48-49	0.45703862253515837
1102	50-51	0.41181196859929514
1102	52-53	0.41618274687861856
1102	54-55	0.4080180659337955
1102	56-57	0.3907754824950871
1102	58-59	0.4137139329087347
1102	60-61	0.34084152544549795
1102	62-63	0.33889416527622274
1102	64-65	0.3069690622483918
1102	66-67	0.35583006373624215
1102	68-69	0.3798313108253666
1102	70-71	0.3492958969410864
1102	72-73	0.35398632199817825
1102	74-75	0.3200346075131151
1102	76-77	0.30805848532940416
1102	78-79	0.30087366487677514
1102	80-81	0.31821437054976087
1102	82-83	0.3034777744887549
1102	84-85	0.21877281414209904
1102	86-87	0.19772448083563177
1102	88-89	0.25281458234640297
1102	90-91	0.2999920978649868
1102	92-93	0.2685215322734962
1102	94-95	0.23532121953358143
1102	96-97	0.26908010313856323
1102	98-99	0.301992339111969
1102	100	0.33169894054639215
1103	1	0.3063328868513189
1103	2	0.29445445644861934
1103	3	0.3938589757825781
1103	4	0.23933033885280963
1103	5	0.23386481462888753
1103	6	0.22152404864753095
1103	7	0.24290571195232502
1103	8	0.18873423333475614
1103	9	0.2573003459073888
1103	10-11	0.2748312346911419
1103	12-13	0.2551148314146019
1103	14-15	0.33847035747909615
1103	16-17	0.3345939566144125
1103	18-19	0.3212342516461604
1103	20-21	0.32633140854823495
1103	22-23	0.3299972080670983
1103	24-25	0.3244020979386306
1103	26-27	0.3336196962708229
1103	28-29	0.3525651972038375
1103	30-31	0.3453521902550776
1103	32-33	0.37665893664308925
1103	34-35	0.3904028343664123
1103	36-37	0.3400197078703471
1103	38-39	0.3363635937838083
1103	40-41	0.3150234299669208
1103	42-43	0.34804826530346133
1103	44-45	0.30707193897791996
1103	46-47	0.26243847293287814
1103	48-49	0.2803141918404606
1103	50-51	0.2748686852939386
1103	52-53	0.2839404450971017
1103	54-55	0.30036625085484303
1103	56-57	0.23292135841443695
1103	58-59	0.2710201369145011
1103	60-61	0.22171474352533238
1103	62-63	0.2894365456342385
1103	64-65	0.21673592929465713
1103	66-67	0.256243382275791
1103	68-69	0.27883357535029063
1103	70-71	0.27738072239760214
1103	72-73	0.32475763943827474
1103	74-75	0.2741074391513152
1103	76-77	0.2837183212689638
1103	78-79	0.2383836791077485
1103	80-81	0.17285776651812768
1103	82-83	0.19282954246686757
1103	84-85	0.18296407777007317
1103	86-87	0.12428959497235326
1103	88-89	0.17753750042008676
1103	90-91	0.20101030411791498
1103	92-93	0.15820541820463063
1103	94-95	0.16039934047487492
1103	96-97	0.1841721721061873
1103	98-99	0.24849707099762597
1103	100	0.22623686255883868
1104	1	0.22722233524789104
1104	2	0.24860724837198944
1104	3	0.32464523063845263
1104	4	0.1809720729649129
1104	5	0.1551139956018659
1104	6	0.16112297882951765
1104	7	0.1415857797216873
1104	8	0.18215934848563364
1104	9	0.21794961902573817
1104	10-11	0.19830602484856996
1104	12-13	0.19683314647542716
1104	14-15	0.2984629258368301
1104	16-17	0.3024746944461896
1104	18-19	0.2543239416952261
1104	20-21	0.2873187213163817
1104	22-23	0.2627937001421117
1104	24-25	0.25899011429136465
1104	26-27	0.26865468917054613
1104	28-29	0.2698724908341248
1104	30-31	0.2688907126540627
1104	32-33	0.3083566847174737
1104	34-35	0.2729397271340517
1104	36-37	0.311249571288279
1104	38-39	0.3318762608683201
1104	40-41	0.2761599977200362
1104	42-43	0.31950810965113163
1104	44-45	0.3592461908026223
1104	46-47	0.34811330490870773
1104	48-49	0.35080835826981627
1104	50-51	0.4074162020533407
1104	52-53	0.3973861573656592
1104	54-55	0.3968677383619976
1104	56-57	0.4004279436110352
1104	58-59	0.38715439822320974
1104	60-61	0.3570271599960506
1104	62-63	0.33555615645049386
1104	64-65	0.33442307707635166
1104	66-67	0.396263355068065
1104	68-69	0.3842050811986297
1104	70-71	0.4032164913779681
1104	72-73	0.3994628954821735
1104	74-75	0.3518328322105191
1104	76-77	0.3409832878544421
1104	78-79	0.35111948469366183
1104	80-81	0.34392400953444735
1104	82-83	0.31207961526189365
1104	84-85	0.3085558501137129
1104	86-87	0.29438485119688096
1104	88-89	0.29739067014072873
1104	90-91	0.27995106310056883
1104	92-93	0.30280401140078084
1104	94-95	0.2928394308017559
1104	96-97	0.2774708032099227
1104	98-99	0.30781046574985993
1104	100	0.3259072971231909
1105	1	0.2482011349131774
1105	2	0.24536122819568362
1105	3	0.3302564515866351
1105	4	0.19914865593865727
1105	5	0.19677271756202686
1105	6	0.14362662662190928
1105	7	0.1761462576788162
1105	8	0.1345074310482559
1105	9	0.2065448108957213
1105	10-11	0.15693858447207276
1105	12-13	0.18558961223921955
1105	14-15	0.2076895474514231
1105	16-17	0.24703733132798078
1105	18-19	0.255774209374124
1105	20-21	0.2532731142221536
1105	22-23	0.20664569317664672
1105	24-25	0.2662416023565015
1105	26-27	0.2605332777260898
1105	28-29	0.2846133722436761
1105	30-31	0.27660435757046997
1105	32-33	0.28228046856335
1105	34-35	0.2481190496274408
1105	36-37	0.2720461106867518
1105	38-39	0.28079425825882964
1105	40-41	0.25472093079576297
1105	42-43	0.2720341656130998
1105	44-45	0.27437163679223175
1105	46-47	0.2696004879615046
1105	48-49	0.2616521453327181
1105	50-51	0.24086902496986795
1105	52-53	0.15896308490074773
1105	54-55	0.19025850362599073
1105	56-57	0.19381714855823873
1105	58-59	0.19663437562084596
1105	60-61	0.14815877364476648
1105	62-63	0.14841875470751376
1105	64-65	0.14602336408782435
1105	66-67	0.19114750439057815
1105	68-69	0.1956420288609877
1105	70-71	0.17310972776840572
1105	72-73	0.17049836233580606
1105	74-75	0.21049994031587715
1105	76-77	0.17211659323078976
1105	78-79	0.18130296500098098
1105	80-81	0.168376135726497
1105	82-83	0.14374425901714005
1105	84-85	0.13666525033684707
1105	86-87	0.14078200228743043
1105	88-89	0.15978049203793177
1105	90-91	0.2204076084988884
1105	92-93	0.192167216635994
1105	94-95	0.12045577466282964
1105	96-97	0.13462450880400567
1105	98-99	0.17179231590555588
1105	100	0.1913539517220606
1106	1	0.20119843240364688
1106	2	0.16380372930775877
1106	3	0.3060624976587363
1106	4	0.13885880035439158
1106	5	0.16443905736998943
1106	6	0.12168180913974425
1106	7	0.12143257371734961
1106	8	0.11210659191041117
1106	9	0.16101851375697152
1106	10-11	0.18036437593949017
1106	12-13	0.16121831137243703
1106	14-15	0.22004012379093751
1106	16-17	0.2531647620946629
1106	18-19	0.21966222605122
1106	20-21	0.19709688864305264
1106	22-23	0.19638612826005186
1106	24-25	0.19436338974664125
1106	26-27	0.20952069546752483
1106	28-29	0.21578582772890087
1106	30-31	0.2560949571275799
1106	32-33	0.2562335843720618
1106	34-35	0.2509475890306945
1106	36-37	0.2398909925228594
1106	38-39	0.20393058905976602
1106	40-41	0.22882519847887295
1106	42-43	0.24353560785255013
1106	44-45	0.2179630686353491
1106	46-47	0.18744044875740684
1106	48-49	0.19904105676202022
1106	50-51	0.18987025622043774
1106	52-53	0.17243435708682853
1106	54-55	0.244616029456175
1106	56-57	0.21923359625081673
1106	58-59	0.1694306665633576
1106	60-61	0.16497326316242322
1106	62-63	0.12698647619512826
1106	64-65	0.13115286130380355
1106	66-67	0.1465520131056124
1106	68-69	0.1636950249038165
1106	70-71	0.1780629636028479
1106	72-73	0.21144103280602167
1106	74-75	0.17760071938630517
1106	76-77	0.12093300554298736
1106	78-79	0.11674208292662769
1106	80-81	0.07322882471823533
1106	82-83	0.1054917416387866
1106	84-85	0.11188864553618316
1106	86-87	0.10167362155929993
1106	88-89	0.03666550129624824
1106	90-91	0.06493255075813664
1106	92-93	0.06059238644166953
1106	94-95	0.0783498014402042
1106	96-97	0.0793424468525572
1106	98-99	0.09083908357384374
1106	100	0.06738432860842636
1107	1	0.1468314204034975
1107	2	0.13368467903339365
1107	3	0.20759576270969404
1107	4	0.11272143812075797
1107	5	0.13773564330214327
1107	6	0.11691225706052677
1107	7	0.07518106341011332
1107	8	0.09434126962254652
1107	9	0.0956842983337225
1107	10-11	0.08966183714738918
1107	12-13	0.12680934230515106
1107	14-15	0.17458009841105593
1107	16-17	0.1696762960878928
1107	18-19	0.1634124863241624
1107	20-21	0.1578851553243723
1107	22-23	0.15471444235170395
1107	24-25	0.16045813460094394
1107	26-27	0.1846793424722577
1107	28-29	0.17834419525066636
1107	30-31	0.18967331290773615
1107	32-33	0.17166510721819606
1107	34-35	0.18378351085817712
1107	36-37	0.18061593461656855
1107	38-39	0.15534713665436328
1107	40-41	0.191282415607688
1107	42-43	0.20655864231993348
1107	44-45	0.2682193268727815
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2216	64-65	-0.6201114141167494
2216	66-67	-0.57725005730925
2216	68-69	-0.6089764646442006
2216	70-71	-0.5871045145174065
2216	72-73	-0.5838903618002007
2216	74-75	-0.5815770070975219
2216	76-77	-0.49675528348574716
2216	78-79	-0.4894677242348635
2216	80-81	-0.5009032397930042
2216	82-83	-0.5172976690962798
2216	84-85	-0.5954138756263063
2216	86-87	-0.5323048869615974
2216	88-89	-0.5361424622533271
2216	90-91	-0.5013546310464179
2216	92-93	-0.5495420633336465
2216	94-95	-0.5650754125575723
2216	96-97	-0.6190785301465951
2216	98-99	-0.7017305199196642
2216	100	-0.7036038007845811
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	578.0
3	76.0
4	76.0
5	181.0
6	596.0
7	1495.0
8	2977.0
9	4977.0
10	6599.0
11	8895.0
12	12925.0
13	18456.0
14	21629.0
15	27391.0
16	34886.0
17	49341.0
18	51042.0
19	27737.0
20	19889.0
21	19032.0
22	19938.0
23	21818.0
24	25011.0
25	31198.0
26	39468.0
27	50632.0
28	66852.0
29	88076.0
30	114564.0
31	149242.0
32	193140.0
33	254800.0
34	350028.0
35	514254.0
36	854877.0
37	1654612.0
38	2486372.0
39	1034466.0
40	3010.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.39824566294281	20.75580486303105	14.766805528452526	23.079143945573612
2	15.515299590758463	21.008757149137843	39.84424176045522	23.631701499648475
3	17.578139374536384	25.88406727597754	31.32243555849946	25.215357790986616
4	12.635961930659414	18.113090015707282	38.5281757859936	30.7227722676397
5	12.454165934721951	37.62002934881396	35.71937826810036	14.206426448363727
6	29.458463259219087	38.784728947164545	17.837883967307096	13.918923826309266
7	25.650140610201795	33.26324612014619	23.025622626234455	18.06099064341756
8	24.366769897021427	35.95953389461207	20.91838216923193	18.755314039134568
9	24.4026124252161	16.845249854257332	22.26489189864445	36.48724582188213
10-11	23.1604345939832	27.204357851026785	29.34426935956508	20.29093819542494
12-13	24.19725325911594	24.890469058976876	29.427024322078704	21.48525335982848
14-15	21.49720692166307	26.978723023080605	27.28230717906109	24.241762876195235
16-17	20.33533886864954	30.144092773681493	27.95819485358914	21.562373504079826
18-19	20.170561288423286	28.307838050360147	31.0342730103947	20.487327650821875
20-21	21.26680882629217	27.66372566678481	30.77886624793491	20.29059925898811
22-23	20.971419669159303	27.55158612568538	30.84891109406745	20.628083111087868
24-25	20.799572843250612	27.580710449507183	30.838385907216637	20.781330800025565
26-27	20.638880657575424	27.54198696159947	30.873895551410847	20.94523682941426
28-29	20.69144001502941	27.527600320343353	31.012544763819406	20.76841490080783
30-31	21.00004164076224	27.906101533735793	30.567944892808928	20.525911932693035
32-33	20.601603701960602	27.72138117566398	30.893529655001444	20.78348546737398
34-35	20.790379192401627	27.407519982723926	30.843124964895868	20.95897585997858
36-37	21.143883843577928	27.63649575554742	30.38259508135443	20.837025319520222
38-39	20.976582397384572	27.749403956066093	30.4828597422921	20.791153904257236
40-41	20.84852494862692	27.54546711251334	30.55197251322336	21.05403542563638
42-43	20.83421698904364	27.58529214384075	30.384755801139214	21.195735065976397
44-45	21.038861967651908	27.78331575705811	30.11538001553297	21.06244225975701
46-47	21.143829371650583	27.9900911932693	29.674895801255417	21.191183633824696
48-49	21.12175008376572	27.8489483770755	29.58613682186082	21.44316471729796
50-51	21.13232369010751	27.859031736071167	29.413370025623593	21.59527454819773
52-53	21.128740647775317	27.990066983523814	29.286038869230573	21.59515349947029
54-55	21.230536575115096	28.328234760933608	29.09647050962483	21.344758154326477
56-57	21.10634058076274	28.00864796318569	29.15278237762942	21.732229078422144
58-59	20.942271135592005	27.638402273004587	29.496312613664756	21.923013977738652
60-61	21.159353870944624	27.994981561857834	29.122762293224564	21.722902273972974
62-63	20.988548064091912	27.907166762537262	29.29132264618329	21.812962527187544
64-65	20.98337928343027	27.711037561904316	29.40139830647988	21.90418484818553
66-67	20.89978908469731	27.792376254306916	29.222088826524583	22.08574583447119
68-69	21.297252581243065	27.941254084184063	28.993954342356787	21.767538992216082
70-71	21.263467881414858	27.643843413302964	29.061650843177016	22.031037862105162
72-73	20.9884209629281	27.73823721095985	29.243677867063315	22.029663959048733
74-75	21.03162930618743	27.784943862442162	29.035353007140905	22.1480738242295
76-77	21.104645898578596	27.823721822277225	28.806595122026803	22.265037157117373
78-79	21.145336428307196	27.80515899992447	28.957766825288918	22.09173774647942
80-81	21.00331462420187	27.605841461521397	28.9989883329949	22.39185558128183
82-83	20.842614295188998	27.644915251467513	29.122686881278458	22.389783572065035
84-85	20.844470359722976	27.536109870021054	28.973132777483695	22.64628699277228
86-87	20.951407600673832	27.64158486709361	28.857016741058526	22.54999079117403
88-89	20.759787238704245	27.496701706034155	28.927366597793586	22.816144457468017
90-91	20.645943934885942	27.40936316626182	28.90691076086605	23.03778213798619
92-93	20.731872283318708	27.527826605941197	28.77271326680114	22.967587843938954
94-95	20.577662011804463	27.28864192721778	28.94703872154726	23.186657339430493
96-97	20.50341837829698	27.335284571111213	28.92194132947008	23.239355721121726
98-99	20.50537081092545	27.291590178140947	28.840535026872516	23.362503984061092
100	20.572377179575764	27.243767980297118	28.715129148254125	23.468725691872997
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1664.0
1	1231.0
2	1601.0
3	2294.5
4	1439.0
5	892.5
6	1961.0
7	4894.0
8	8189.5
9	9529.5
10	7552.5
11	4962.0
12	4623.0
13	5148.5
14	5891.5
15	6500.0
16	7185.5
17	8176.0
18	9317.0
19	10611.5
20	12301.0
21	14925.5
22	18946.0
23	25139.0
24	33334.5
25	43987.5
26	59052.5
27	78782.0
28	103961.5
29	130872.5
30	155022.5
31	177962.0
32	200560.5
33	220782.5
34	238671.0
35	254973.0
36	268845.0
37	280803.5
38	292204.5
39	299345.5
40	303117.0
41	304058.5
42	299216.5
43	294125.5
44	289286.5
45	284860.5
46	278829.0
47	268992.0
48	261424.5
49	256109.0
50	248631.5
51	236012.0
52	218585.5
53	205428.5
54	196524.5
55	186582.0
56	172513.0
57	154987.0
58	139090.5
59	124998.0
60	108709.5
61	89813.0
62	73516.0
63	59570.5
64	47335.5
65	37313.5
66	28980.5
67	22108.0
68	16686.0
69	12378.5
70	8874.0
71	6195.0
72	4349.0
73	3054.5
74	2120.0
75	1373.5
76	814.5
77	537.5
78	333.0
79	191.0
80	99.0
81	40.0
82	19.0
83	11.0
84	11.0
85	6.0
86	4.0
87	3.5
88	2.0
89	2.0
90	1.0
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	1.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12983686505100508
2	0.0
3	0.0
4	0.0
5	0.014247435219563023
6	1.9367796390230106E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	2.239401457620356E-4
82-83	5.93138764450797E-4
84-85	3.5104130957292075E-4
86-87	4.781424733838058E-4
88-89	5.689290189630095E-4
90-91	7.626069828653106E-4
92-93	2.723596367376109E-4
94-95	3.0262181859734544E-4
96-97	6.6576800091416E-4
98-99	0.001125753165182125
100	1.2104872743893817E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	8261136.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.84947770893037
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.94204680198774	42.775087882242765
2	15.63391524356748	18.08827662772127
3	4.880230014902987	8.469562723847499
4	1.978559754215193	4.578345935890342
5	0.9712334906885703	2.809267508487754
6	0.604283514900712	2.09744914350737
7	0.39656919263104323	1.6058924468410631
8	0.28804263939762825	1.333049299764365
9	0.2021864222835267	1.0526741036045335
>10	1.0164173358610793	10.47627623305968
>50	0.05902319346075827	2.323798634781466
>100	0.02566954890718638	2.643968328898814
>500	0.0012782953253552781	0.48418563528894903
>1k	4.6077467252293155E-4	0.45857277554445214
>5k	2.0944299569682772E-5	0.08225666394679619
>10k+	6.283289870904833E-5	0.7213360565727664
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23679	0.2866312817026617	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23084	0.2794288824200449	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12772	0.15460343468501184	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.2104872743893818E-5	0.0
3	0.0	0.0	0.0	2.4209745487787636E-5	0.0
4	0.0	0.0	0.0	2.4209745487787636E-5	0.0
5	0.0	0.0	0.0	6.052436371946909E-5	0.0
6	0.0	0.0	0.0	6.052436371946909E-5	0.0
7	0.0	0.0	0.0	6.052436371946909E-5	0.0
8	0.0	0.0	0.0	6.052436371946909E-5	0.0
9	2.4209745487787636E-5	0.0	0.0	9.683898195115054E-5	0.0
10-11	2.4209745487787636E-5	0.0	0.0	1.0894385469504436E-4	0.0
12-13	2.4209745487787636E-5	0.0	0.0	1.2104872743893818E-4	0.0
14-15	2.4209745487787636E-5	0.0	0.0	1.4525847292672583E-4	0.0
16-17	2.4209745487787636E-5	0.0	0.0	1.5736334567061964E-4	0.0
18-19	2.4209745487787636E-5	0.0	0.0	2.1788770939008871E-4	0.0
20-21	3.0262181859734546E-5	0.0	0.0	3.0262181859734544E-4	0.0
22-23	3.631461823168146E-5	0.0	0.0	6.476106917983192E-4	0.0
24-25	3.631461823168146E-5	6.052436371946909E-6	0.0	0.0011862775289015943	0.0
26-27	3.631461823168146E-5	1.2104872743893818E-5	0.0	0.002233349021248409	0.0
28-29	4.841949097557527E-5	1.2104872743893818E-5	0.0	0.005707447498745935	0.0
30-31	6.052436371946909E-5	1.2104872743893818E-5	0.0	0.01570607238520223	0.0
32-33	6.052436371946909E-5	1.815730911584073E-5	0.0	0.04054527125567234	0.0
34-35	7.262923646336291E-5	2.4209745487787636E-5	0.0	0.08354783167835514	0.0
36-37	7.262923646336291E-5	3.631461823168146E-5	0.0	0.1487144140951075	0.0
38-39	7.262923646336291E-5	5.447192734752218E-5	0.0	0.2344532277401074	0.0
40-41	7.868167283530982E-5	7.262923646336291E-5	0.0	0.3214933152050759	0.0
42-43	8.473410920725673E-5	8.473410920725673E-5	0.0	0.4144224232599487	0.0
44-45	8.473410920725673E-5	8.473410920725673E-5	0.0	0.5336614722236748	0.0
46-47	8.473410920725673E-5	9.683898195115054E-5	0.0	0.6791680950416504	0.0
48-49	8.473410920725673E-5	9.683898195115054E-5	0.0	0.8313323978687677	0.0
50-51	8.473410920725673E-5	1.2104872743893818E-4	0.0	0.9830790825862206	0.0
52-53	8.473410920725673E-5	1.2104872743893818E-4	0.0	1.1344928833032164	0.0
54-55	8.473410920725673E-5	1.2104872743893818E-4	0.0	1.3111877107458345	0.0
56-57	9.078654557920364E-5	1.2104872743893818E-4	0.0	1.5229140399092813	0.0
58-59	1.2104872743893818E-4	1.3315360018283199E-4	0.0	1.756647027721127	0.0
60-61	1.271011638108851E-4	1.3315360018283199E-4	0.0	1.9961298300863222	0.0
62-63	1.3315360018283199E-4	1.3315360018283199E-4	0.0	2.2399703866393192	0.0
64-65	1.3315360018283199E-4	1.6946821841451346E-4	0.0	2.507899639952665	0.0
66-67	1.3315360018283199E-4	1.6946821841451346E-4	0.0	2.8205866602365584	0.0
68-69	1.3315360018283199E-4	1.7552065478646038E-4	0.0	3.1629003565611313	0.0
70-71	1.3315360018283199E-4	1.8157309115840727E-4	0.0	3.526621520333281	0.0
72-73	1.3315360018283199E-4	1.9367796390230108E-4	0.0	3.90821552871179	6.052436371946909E-6
74-75	1.3315360018283199E-4	1.99730400274248E-4	0.0	4.2957711869166655	1.2104872743893818E-5
76-77	1.3315360018283199E-4	2.2999258213398256E-4	0.0	4.712608532289021	1.2104872743893818E-5
78-79	1.3315360018283199E-4	2.2999258213398256E-4	0.0	5.18787004595978	1.2104872743893818E-5
80-81	1.3315360018283199E-4	2.2999258213398256E-4	0.0	5.69599023669384	2.4209745487787636E-5
82-83	1.3315360018283199E-4	2.2999258213398256E-4	0.0	6.2157129479529205	2.4209745487787636E-5
84-85	1.392060365547789E-4	2.2999258213398256E-4	0.0	6.734866730193039	2.4209745487787636E-5
86-87	1.6946821841451346E-4	2.2999258213398256E-4	0.0	7.283592716546489	2.4209745487787636E-5
88	1.8157309115840727E-4	2.542023276217702E-4	0.0	7.72522084129834	2.4209745487787636E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14355	0.0	57.221786	1
GTATCAA	26255	0.0	40.19692	1
ATCAACG	32325	0.0	32.196484	3
TCAACGC	32605	0.0	32.006493	4
GTGGTAT	6050	0.0	31.667095	1
TATCAAC	33880	0.0	30.899124	2
CAACGCA	33890	0.0	30.79291	5
AACGCAG	35655	0.0	29.36838	6
TGGTATC	6525	0.0	28.024534	2
ACGCAGA	40395	0.0	25.805908	7
CGCAGAG	40495	0.0	25.556486	8
GCAGAGT	44510	0.0	22.871048	9
GTACATG	21625	0.0	22.028936	1
GAGTACT	31840	0.0	21.403273	12-13
TACATGG	21945	0.0	20.94945	2
CAGAGTA	43095	0.0	20.710152	10-11
AGAGTAC	40905	0.0	20.629763	10-11
ACATGGG	22225	0.0	19.691431	3
GTACTTT	35505	0.0	19.140972	14-15
AGTACTT	33460	0.0	18.871088	12-13
>>END_MODULE