FastQCFastQC Report
Wed 25 May 2016
SRR2049445_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049445_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5132528
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53070.10339933849362341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57400.047.5955241
GTATCAA97650.035.3448141
ATCAACG105150.031.4662593
TCAACGC105750.031.2877274
CAACGCA112700.029.4011215
TATCAAC118100.028.2941862
AACGCAG118500.027.8047836
ACGCAGA136450.024.0092757
CGCAGAG136700.023.620388
GTACATG157250.023.114831
TACATGG156300.022.2811052
GTACCTG35950.021.974161
ACATGGG159600.021.1138783
TACCTGG36900.021.0152742
GTGGTAT33600.020.4322151
TGGTATC33550.019.6115992
GCAGAGT160950.019.2435259
CAGAGTA151950.017.66176410-11
ATAACGC4550.017.5597883
AGAGTAC140050.017.26636510-11
Produced by FastQC (version 0.11.5)