##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049445_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5132528
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.75679509200924	31.0	31.0	34.0	28.0	34.0
2	30.94207591268864	31.0	31.0	34.0	28.0	34.0
3	31.015246287989076	31.0	31.0	34.0	28.0	34.0
4	34.679284555291275	37.0	35.0	37.0	32.0	37.0
5	34.72264350043487	37.0	35.0	37.0	32.0	37.0
6	34.83686109457172	37.0	35.0	37.0	32.0	37.0
7	34.77509055966183	37.0	35.0	37.0	32.0	37.0
8	34.71842394235355	37.0	35.0	37.0	32.0	37.0
9	36.319929087576334	39.0	37.0	39.0	32.0	39.0
10-11	36.25643026204631	39.0	37.0	39.0	32.0	39.0
12-13	36.17991543348619	39.0	37.0	39.0	32.0	39.0
14-15	37.421128730325485	40.0	38.0	41.0	32.0	41.0
16-17	37.40500295371014	40.0	38.0	41.0	32.0	41.0
18-19	37.41984018401848	40.0	38.0	41.0	32.0	41.0
20-21	37.471658800497536	40.0	38.0	41.0	32.0	41.0
22-23	37.4373557241188	40.0	38.0	41.0	32.0	41.0
24-25	37.44519201843614	40.0	38.0	41.0	32.0	41.0
26-27	37.4502710944782	40.0	38.0	41.0	32.0	41.0
28-29	37.436171902033465	40.0	38.0	41.0	32.0	41.0
30-31	37.38743558729733	40.0	38.0	41.0	32.0	41.0
32-33	37.34958893551092	40.0	38.0	41.0	32.0	41.0
34-35	37.29421573540368	40.0	38.0	41.0	31.0	41.0
36-37	37.1888765146532	40.0	38.0	41.0	31.0	41.0
38-39	37.0902120748294	40.0	37.0	41.0	31.0	41.0
40-41	36.97875803113008	40.0	37.0	41.0	30.0	41.0
42-43	36.89076874008286	40.0	37.0	41.0	30.0	41.0
44-45	36.74452014679706	40.0	37.0	41.0	30.0	41.0
46-47	36.627557998709406	40.0	37.0	41.0	30.0	41.0
48-49	36.536797071540576	40.0	36.0	41.0	30.0	41.0
50-51	36.434301965814896	40.0	36.0	41.0	30.0	41.0
52-53	36.33029035594156	40.0	36.0	41.0	30.0	41.0
54-55	36.19711621641421	40.0	36.0	41.0	29.0	41.0
56-57	36.04995101828962	39.0	35.0	41.0	29.0	41.0
58-59	35.880285991620504	39.0	35.0	41.0	29.0	41.0
60-61	35.69751377878504	39.0	35.0	41.0	28.5	41.0
62-63	35.45353965920887	39.0	35.0	40.5	28.0	41.0
64-65	35.21957493461312	38.0	35.0	40.0	28.0	41.0
66-67	34.99901150076531	38.0	34.5	40.0	28.0	41.0
68-69	34.71807538117668	37.0	34.0	40.0	28.0	41.0
70-71	34.40510475539539	37.0	34.0	40.0	27.5	41.0
72-73	34.0711102793789	36.0	34.0	39.0	26.5	41.0
74-75	33.769356640626214	36.0	34.0	39.0	26.0	41.0
76-77	33.441131836007514	35.5	34.0	38.5	26.0	40.0
78-79	33.08980262747714	35.0	33.5	37.5	26.0	39.5
80-81	32.75423495010646	35.0	33.0	37.0	26.0	39.0
82-83	32.45209183466705	35.0	33.0	36.5	26.0	39.0
84-85	32.160381102645715	35.0	33.0	36.0	25.0	37.5
86-87	31.88842428136778	35.0	33.0	36.0	25.0	37.0
88-89	31.657342151859666	35.0	33.0	35.5	25.0	37.0
90-91	31.450928178083004	35.0	33.0	35.0	24.0	36.0
92-93	31.25170754061157	35.0	33.0	35.0	24.0	36.0
94-95	31.056501104329094	35.0	32.0	35.0	23.0	36.0
96-97	30.875589767849295	35.0	32.0	35.0	20.0	36.0
98-99	30.710719649264455	35.0	32.0	35.0	18.5	35.5
100	30.587596404734665	35.0	32.0	35.0	18.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.21284245518330636
1101	2	0.15991285727132265
1101	3	0.18495728730532335
1101	4	0.35013692214427294
1101	5	0.29205096343181225
1101	6	0.3555312399157273
1101	7	0.42638582524961777
1101	8	0.38095662889664794
1101	9	0.545622353364493
1101	10-11	0.4956154273532931
1101	12-13	0.49889237172322964
1101	14-15	0.5940699146063082
1101	16-17	0.5875305829697979
1101	18-19	0.5629037192168838
1101	20-21	0.5368951369023378
1101	22-23	0.5685002578991174
1101	24-25	0.5759182282805924
1101	26-27	0.55414348907766
1101	28-29	0.5222840065129617
1101	30-31	0.5851696044032053
1101	32-33	0.5244553006433321
1101	34-35	0.5181996609192865
1101	36-37	0.5492544967093593
1101	38-39	0.5403336897541209
1101	40-41	0.5961466992653115
1101	42-43	0.5068779888361377
1101	44-45	0.5012544315983405
1101	46-47	0.44468843540429503
1101	48-49	0.4230221731215451
1101	50-51	0.48363942849418606
1101	52-53	0.45885875005473764
1101	54-55	0.4441184693560132
1101	56-57	0.43999674605298367
1101	58-59	0.4310854644381621
1101	60-61	0.47951966444745864
1101	62-63	0.45565204403087733
1101	64-65	0.44249273442918735
1101	66-67	0.4159547096843994
1101	68-69	0.4185026560049465
1101	70-71	0.43604945498985614
1101	72-73	0.429137405496526
1101	74-75	0.3378602849440213
1101	76-77	0.328089254400993
1101	78-79	0.34154207579569373
1101	80-81	0.4278317298460408
1101	82-83	0.4038903307900128
1101	84-85	0.30946109289153867
1101	86-87	0.3533684480199142
1101	88-89	0.2743971589748284
1101	90-91	0.32675204289086324
1101	92-93	0.25254421204699184
1101	94-95	0.31561435714770525
1101	96-97	0.2773972626060086
1101	98-99	0.289697388368527
1101	100	0.2767980966916461
1102	1	0.04302760894550417
1102	2	0.0781700099190239
1102	3	0.10089300277476809
1102	4	0.151017975559121
1102	5	0.1958932192666012
1102	6	0.24646834190908606
1102	7	0.28807940478558436
1102	8	0.2921295521974443
1102	9	0.38577438571143574
1102	10-11	0.3642613926677498
1102	12-13	0.3586463446094257
1102	14-15	0.4945747331070294
1102	16-17	0.41457736461671857
1102	18-19	0.46550111591919574
1102	20-21	0.43027904094431335
1102	22-23	0.44975905159690655
1102	24-25	0.4045137076184133
1102	26-27	0.349267579666531
1102	28-29	0.3731605537250928
1102	30-31	0.3682088598662716
1102	32-33	0.30735730674535233
1102	34-35	0.30420459199253713
1102	36-37	0.3637529893597886
1102	38-39	0.37091916103030087
1102	40-41	0.401551410739593
1102	42-43	0.34860083723162916
1102	44-45	0.25768583927004585
1102	46-47	0.31652398343447175
1102	48-49	0.2656118878211444
1102	50-51	0.2519829747865856
1102	52-53	0.24501983013045248
1102	54-55	0.303587585856377
1102	56-57	0.27092422656076565
1102	58-59	0.22085633475921185
1102	60-61	0.21305535324282232
1102	62-63	0.17117220672955114
1102	64-65	0.23458446421020085
1102	66-67	0.23152400319764865
1102	68-69	0.24014991738913238
1102	70-71	0.18578110484106958
1102	72-73	0.2093403942478531
1102	74-75	0.20118930244657207
1102	76-77	0.21409825426168538
1102	78-79	0.24518679323863068
1102	80-81	0.20935711577836713
1102	82-83	0.1935919161810702
1102	84-85	0.22232545156811057
1102	86-87	0.18794946030121906
1102	88-89	0.1140397263153261
1102	90-91	0.10323790959045454
1102	92-93	0.1165445382102348
1102	94-95	0.1362729361983881
1102	96-97	0.15491995207945308
1102	98-99	0.11921845494874361
1102	100	0.10888592439822631
1103	1	0.1644699686569986
1103	2	0.15016651923275504
1103	3	0.17327209521844011
1103	4	0.23942982302282445
1103	5	0.27216661647060647
1103	6	0.2773609824683234
1103	7	0.31437644999985537
1103	8	0.27815565799055975
1103	9	0.4431110822296347
1103	10-11	0.46200985306072795
1103	12-13	0.4630310673475506
1103	14-15	0.5831783555938586
1103	16-17	0.5396985152834333
1103	18-19	0.49951035466638416
1103	20-21	0.478451640162163
1103	22-23	0.5074557863086966
1103	24-25	0.481058127516782
1103	26-27	0.49443459255500954
1103	28-29	0.5037003724436744
1103	30-31	0.4628649245784686
1103	32-33	0.4677431383194275
1103	34-35	0.41076935581001095
1103	36-37	0.4133835503717336
1103	38-39	0.4523804884824685
1103	40-41	0.47731543051298075
1103	42-43	0.43218560169318465
1103	44-45	0.43278445684020284
1103	46-47	0.38139452675934393
1103	48-49	0.40416839977751806
1103	50-51	0.4003169543094316
1103	52-53	0.3753144566331841
1103	54-55	0.28307315401876565
1103	56-57	0.2872387982184179
1103	58-59	0.3755410797877303
1103	60-61	0.4343748001319696
1103	62-63	0.41276971388512607
1103	64-65	0.36098280278751105
1103	66-67	0.36696993552676815
1103	68-69	0.40244517187440465
1103	70-71	0.36270278390978916
1103	72-73	0.4002037435575403
1103	74-75	0.3683309289276693
1103	76-77	0.3107703888071782
1103	78-79	0.36889101685535053
1103	80-81	0.3794374081138727
1103	82-83	0.34035626494280535
1103	84-85	0.36663345798243085
1103	86-87	0.32397984420567383
1103	88-89	0.3294888664386306
1103	90-91	0.3193032615298357
1103	92-93	0.28144402390547896
1103	94-95	0.318497746686738
1103	96-97	0.333577423516747
1103	98-99	0.26963062374438707
1103	100	0.23926160414038833
1104	1	0.04904788422573958
1104	2	0.06257271785903029
1104	3	0.050561987328421765
1104	4	0.17552698229469854
1104	5	0.20436020495426988
1104	6	0.2877105299445475
1104	7	0.25768081545658816
1104	8	0.26000080354486954
1104	9	0.31855893437771243
1104	10-11	0.34974803989407377
1104	12-13	0.3979082035298589
1104	14-15	0.4221285367471097
1104	16-17	0.41601015524048535
1104	18-19	0.3740411586630259
1104	20-21	0.37983984186150366
1104	22-23	0.3447849408991175
1104	24-25	0.3456478677893884
1104	26-27	0.3976358682309922
1104	28-29	0.3592438076147175
1104	30-31	0.31667512900286
1104	32-33	0.35873261751044083
1104	34-35	0.3372640781942593
1104	36-37	0.2806383598790063
1104	38-39	0.3641721316018618
1104	40-41	0.34323030550627465
1104	42-43	0.37611414015827194
1104	44-45	0.3456818951236471
1104	46-47	0.3192467996671553
1104	48-49	0.2926147259865246
1104	50-51	0.2817720283783771
1104	52-53	0.27939979536214565
1104	54-55	0.2798887491614863
1104	56-57	0.19679234377605326
1104	58-59	0.23952171163393388
1104	60-61	0.22525736145512099
1104	62-63	0.25028731287257244
1104	64-65	0.2558503763174187
1104	66-67	0.26304547480664553
1104	68-69	0.29917332057061685
1104	70-71	0.25605591775892833
1104	72-73	0.22985906819219082
1104	74-75	0.24683866907772511
1104	76-77	0.2157847195463347
1104	78-79	0.22317559011820975
1104	80-81	0.2740686105039316
1104	82-83	0.2363851257691536
1104	84-85	0.1800343729406393
1104	86-87	0.1987453927834153
1104	88-89	0.18066877405009052
1104	90-91	0.22577531044159116
1104	92-93	0.22390935929054478
1104	94-95	0.24436754898041002
1104	96-97	0.3006289793769348
1104	98-99	0.28216217141882893
1104	100	0.2260725579166163
1105	1	-0.0010436025581306296
1105	2	-0.027443259602346615
1105	3	0.007752535827325602
1105	4	0.09632277186636884
1105	5	0.14772839489770462
1105	6	0.14444589896790916
1105	7	0.15533517576906775
1105	8	0.18450638947481224
1105	9	0.22722537996344272
1105	10-11	0.27864953669931225
1105	12-13	0.26525383958117743
1105	14-15	0.27094066303668285
1105	16-17	0.24043096317496548
1105	18-19	0.314548477605733
1105	20-21	0.3243373081575669
1105	22-23	0.26627242655430194
1105	24-25	0.18912183478142452
1105	26-27	0.26104359093731944
1105	28-29	0.21833090696005542
1105	30-31	0.2831566585629872
1105	32-33	0.2616783210496223
1105	34-35	0.2567667078938669
1105	36-37	0.26238749191814037
1105	38-39	0.2662538559168439
1105	40-41	0.30563465527377076
1105	42-43	0.2862807181016649
1105	44-45	0.25361593737046917
1105	46-47	0.21247463230306352
1105	48-49	0.21256357192947206
1105	50-51	0.18215797461207472
1105	52-53	0.18669508459274198
1105	54-55	0.11020523670894988
1105	56-57	0.1253138395340656
1105	58-59	0.09440116434225132
1105	60-61	0.09358175337344932
1105	62-63	0.08257094808320886
1105	64-65	0.10669639271522868
1105	66-67	0.11368524595878426
1105	68-69	0.04240393489928351
1105	70-71	0.09314393633554374
1105	72-73	0.016217974190610107
1105	74-75	0.08133731350248752
1105	76-77	0.03876010174728606
1105	78-79	0.09428218385725273
1105	80-81	0.08406892321710302
1105	82-83	0.07582256568094436
1105	84-85	0.06729676547276142
1105	86-87	0.06863667768547543
1105	88-89	0.07787997252695789
1105	90-91	0.12109008533901289
1105	92-93	0.053208565435433286
1105	94-95	0.11109834002837715
1105	96-97	0.10938952981029715
1105	98-99	0.07202519214080283
1105	100	0.030500430987064675
1106	1	0.18441856534961332
1106	2	0.199538640151534
1106	3	0.16843879985800214
1106	4	0.32624123727512
1106	5	0.2955922947954974
1106	6	0.3174930263947857
1106	7	0.34704444008547597
1106	8	0.3208394077357397
1106	9	0.4169948880802252
1106	10-11	0.3895387115519142
1106	12-13	0.43608265815745995
1106	14-15	0.4900752688154313
1106	16-17	0.45977817296778056
1106	18-19	0.42714719971117177
1106	20-21	0.43557163051087144
1106	22-23	0.4220497961118781
1106	24-25	0.4666215780428189
1106	26-27	0.41672067525306034
1106	28-29	0.4281699510680994
1106	30-31	0.4379935963665744
1106	32-33	0.4101756492216353
1106	34-35	0.422125422370506
1106	36-37	0.38922394139443384
1106	38-39	0.378561572675828
1106	40-41	0.43657181404721257
1106	42-43	0.3620393370086674
1106	44-45	0.32401544359402124
1106	46-47	0.3683303158669773
1106	48-49	0.3771850973130597
1106	50-51	0.29095838008441177
1106	52-53	0.3026017875311595
1106	54-55	0.2812419611575194
1106	56-57	0.23268957281095481
1106	58-59	0.29819818854972624
1106	60-61	0.31357273084542925
1106	62-63	0.3101986764111473
1106	64-65	0.2811467275931889
1106	66-67	0.310664445591037
1106	68-69	0.3068230559520586
1106	70-71	0.27486481152426023
1106	72-73	0.24492403957896158
1106	74-75	0.25687319857301105
1106	76-77	0.20234349498629456
1106	78-79	0.18005621057344
1106	80-81	0.2025785713489867
1106	82-83	0.20345893928836034
1106	84-85	0.2237039654710884
1106	86-87	0.18965370137598825
1106	88-89	0.22521343650006997
1106	90-91	0.1860809126101728
1106	92-93	0.08897164146429049
1106	94-95	0.12261020205041717
1106	96-97	0.15602784345335152
1106	98-99	0.20595686613936337
1106	100	0.2586937876399489
1107	1	0.1612431383923898
1107	2	0.18351349983877796
1107	3	0.2131514486666184
1107	4	0.16787931388632416
1107	5	0.20704650911507372
1107	6	0.26929888318008466
1107	7	0.2531563210372312
1107	8	0.2825975794870743
1107	9	0.26038502280346165
1107	10-11	0.2625757362654966
1107	12-13	0.2891978583284214
1107	14-15	0.32945968485129384
1107	16-17	0.27844098682436424
1107	18-19	0.2943843994479636
1107	20-21	0.2721201362197476
1107	22-23	0.2580354446141442
1107	24-25	0.32237735979401094
1107	26-27	0.3875273882183805
1107	28-29	0.383276276994053
1107	30-31	0.3981212557827547
1107	32-33	0.40283688124169004
1107	34-35	0.3833683475575498
1107	36-37	0.4004518022766703
1107	38-39	0.392861993800075
1107	40-41	0.4452697001752668
1107	42-43	0.4453048556096135
1107	44-45	0.42992501313024434
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2213	98-99	-0.7453947739282505
2213	100	-0.7288353924566842
2214	1	-0.2813754815961289
2214	2	-0.30528035497911077
2214	3	-0.268528755579311
2214	4	-0.3753419056241398
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2214	30-31	-0.5936497777316916
2214	32-33	-0.7477116396490828
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2214	82-83	-0.5163016129910076
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2214	86-87	-0.6096531743952198
2214	88-89	-0.6446775858768845
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2214	94-95	-0.5030382134852474
2214	96-97	-0.46740918761926054
2214	98-99	-0.4565875121939662
2214	100	-0.47265260033812595
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2215	2	-0.4274833490124834
2215	3	-0.3742860732993627
2215	4	-0.4168946966126228
2215	5	-0.33029196273633943
2215	6	-0.47750273702180834
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2215	28-29	-0.7477049929708457
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2215	94-95	-0.5777223238708018
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2215	98-99	-0.6399124650455299
2215	100	-0.6460965517265471
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2216	2	-0.3810877813589535
2216	3	-0.4168124745803503
2216	4	-0.5693959566824418
2216	5	-0.510440723896636
2216	6	-0.6339952637352724
2216	7	-0.5210458035716172
2216	8	-0.5453580571455774
2216	9	-0.7518058971637771
2216	10-11	-0.7181536478703805
2216	12-13	-0.793572708998191
2216	14-15	-0.9005853740232368
2216	16-17	-0.9341629104750453
2216	18-19	-1.0286407874902253
2216	20-21	-0.9226185255263957
2216	22-23	-0.9562231980680025
2216	24-25	-0.9510042553192335
2216	26-27	-1.0060853545712973
2216	28-29	-1.0209203732877796
2216	30-31	-1.0756623541740424
2216	32-33	-1.0961258890709118
2216	34-35	-1.0382826542888566
2216	36-37	-1.0247768913721913
2216	38-39	-0.9404259195730305
2216	40-41	-0.9618344900409355
2216	42-43	-0.9713428008758527
2216	44-45	-0.9485725243853693
2216	46-47	-0.8319794707940176
2216	48-49	-0.9941346808661606
2216	50-51	-0.9097780943930545
2216	52-53	-0.988625925384035
2216	54-55	-1.0075807038245586
2216	56-57	-0.9284403866967494
2216	58-59	-0.88228591028723
2216	60-61	-0.9205411880976939
2216	62-63	-0.9351189587670206
2216	64-65	-0.9237635062175329
2216	66-67	-0.9668657835290588
2216	68-69	-0.8639569440311305
2216	70-71	-0.8399145963876364
2216	72-73	-0.8529563003758724
2216	74-75	-0.8869585282100445
2216	76-77	-0.9176171531482282
2216	78-79	-0.9249994281560348
2216	80-81	-0.8957008423070931
2216	82-83	-0.888822257026284
2216	84-85	-0.7936071636478097
2216	86-87	-0.9200364923487285
2216	88-89	-0.9138791491029856
2216	90-91	-0.9526292924516042
2216	92-93	-0.9500564831828306
2216	94-95	-0.9114543589383928
2216	96-97	-1.004146388864438
2216	98-99	-0.9991374337524483
2216	100	-1.0936706867377666
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	84397.0
3	15558.0
4	9844.0
5	7808.0
6	6332.0
7	6242.0
8	6548.0
9	7322.0
10	8463.0
11	9397.0
12	10596.0
13	11978.0
14	13603.0
15	14435.0
16	14752.0
17	16001.0
18	15218.0
19	13643.0
20	13840.0
21	15049.0
22	17222.0
23	21354.0
24	25722.0
25	31398.0
26	37586.0
27	42686.0
28	51383.0
29	64752.0
30	78038.0
31	90674.0
32	116622.0
33	155962.0
34	216626.0
35	315739.0
36	525989.0
37	994189.0
38	1450766.0
39	593340.0
40	1454.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.000106463110235	20.93164190879387	14.493295066409779	22.57495656168611
2	15.40484189030141	21.12678004941659	40.559561018574094	22.908817041707913
3	17.87108712749812	26.47057906498726	30.81231930770052	24.846014499814096
4	12.43219228464675	18.18356480692131	39.54818564003138	29.836057268400566
5	12.58038412128267	38.17577171971504	35.58422837381435	13.659615785187945
6	29.00195960108079	38.868941716043146	18.742648086081378	13.386450596794688
7	25.54430679211081	33.22180707613933	23.498658391547	17.735227740202866
8	23.896757589568512	36.449238777984036	21.56473879873587	18.089264833711574
9	24.237884156092456	17.878450782249082	22.592677678616838	35.290987383041625
10-11	22.821447598099258	27.812928326267066	29.71962203428208	19.646002041351597
12-13	23.44266768957031	25.6847285351241	29.443168683715342	21.429435091590246
14-15	21.129696677298526	27.988659132718468	26.720805431114492	24.160838758868515
16-17	20.4682019887726	31.054058051302157	27.30222807925862	21.17551188066663
18-19	20.341675314045755	30.00373434277175	29.327558173373465	20.32703216980903
20-21	21.648264346473244	29.272822719043408	28.766048484071405	20.312864450411947
22-23	21.257255864952494	29.281532723953376	28.768017509362547	20.69319390173159
24-25	20.986170217533935	29.35655880419763	28.785155398225676	20.872115580042756
26-27	20.827489173630216	29.215917625381643	28.856952993714952	21.099640207273186
28-29	20.813820690036827	29.24536758010487	28.994711345382996	20.94610038447531
30-31	21.167373957599175	29.6918973278179	28.548761375387443	20.59196733919548
32-33	20.6712342887719	29.364941262712836	28.994982188923867	20.96884225959139
34-35	20.887218918285384	29.03442123905393	28.991173776468976	21.087186066191705
36-37	21.293924201411592	29.42504869194975	28.464869066370408	20.816158040268252
38-39	21.080331511189073	29.479743272627996	28.63473251914148	20.805192697041456
40-41	20.927019413281517	29.1753598424287	28.85914161599829	21.038479128291495
42-43	20.824769205753302	29.27150202903696	28.744915960078444	21.158812805131298
44-45	21.062088626323842	29.37487327077022	28.591283484408052	20.971754618497883
46-47	21.046317302479554	29.507920070870046	28.44368064359856	21.00208198305183
48-49	20.907302980773682	29.293846262523417	28.64061422888893	21.15823652781398
50-51	21.021433143576438	29.164624742181662	28.571922669599893	21.242019444642008
52-53	21.017405644931312	29.18824092317504	28.51197231792107	21.282381113972576
54-55	21.18216646991042	29.547760715177528	28.336981532192855	20.93309128271919
56-57	21.009005424027997	29.264310359594337	28.389335437453845	21.337348778923822
58-59	20.900447625620224	28.983008775207924	28.685838671627035	21.430704927544816
60-61	21.209000661710206	29.346759919223032	28.334904472895992	21.109334946170772
62-63	21.053716661044856	29.347819097286976	28.409999460228065	21.188464781440107
64-65	21.1062587854886	29.110556305956486	28.512076106569623	21.271108801985296
66-67	20.968346316546967	29.163213580668145	28.41773672548653	21.450703377298353
68-69	21.329771759025515	29.354255503854098	28.30698429580345	21.00898844131694
70-71	21.365599596590506	29.09135909534934	28.427087875547198	21.115953432512953
72-73	21.05612545161881	29.260475412839032	28.61622436929265	21.067174766249508
74-75	21.116647154913576	29.235407230505178	28.454225816996807	21.19371979758444
76-77	21.18410474107208	29.294495801479563	28.249025827126257	21.272373630322097
78-79	21.323904583499083	29.334668884531233	28.326477770155613	21.014948761814072
80-81	21.130174542694437	29.174055999621945	28.46073386173093	21.235035595952688
82-83	21.08212746960845	29.26813988558316	28.54519648234185	21.10453616246653
84-85	21.10527427500494	29.152726265138934	28.47018082348948	21.27181863636665
86-87	21.255751854472805	29.35620249333824	28.296556638137215	21.09148901405174
88-89	21.08390201764429	29.255730175845656	28.354399522273027	21.305968284237025
90-91	20.927435348642977	29.171024726237537	28.482481355624383	21.4190585694951
92-93	21.101075635891316	29.440590616717007	28.25761131788048	21.200722429511195
94-95	21.031446195271393	29.18906758307643	28.43980302685304	21.33968319479914
96-97	21.071060772134942	29.271426135671657	28.375574229691875	21.281938862501523
98-99	21.002847798193113	29.315495704920174	28.321026649272657	21.360629847614053
100	21.12878809873194	29.24772985404388	28.32277799655077	21.30070405067341
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4893.0
1	3603.0
2	2690.0
3	2983.5
4	2659.5
5	2228.5
6	1979.5
7	1918.5
8	1917.5
9	1882.5
10	1643.5
11	1420.5
12	1404.5
13	1449.5
14	1532.5
15	1699.0
16	1950.0
17	2354.0
18	3124.0
19	4240.5
20	5791.0
21	8266.0
22	12237.5
23	17970.5
24	25488.0
25	35005.0
26	47232.5
27	61538.0
28	77578.5
29	94743.0
30	110459.5
31	124715.5
32	137962.0
33	149088.5
34	158608.0
35	168276.0
36	176555.0
37	182446.0
38	187529.5
39	189757.0
40	190169.5
41	188374.0
42	183427.5
43	179245.0
44	174517.0
45	169010.5
46	164448.5
47	159150.5
48	154747.5
49	151209.0
50	146501.0
51	139046.0
52	128158.5
53	118011.0
54	110832.5
55	105703.0
56	99009.0
57	89473.5
58	80154.5
59	72221.0
60	62913.5
61	52090.0
62	42829.5
63	34920.0
64	27929.5
65	22400.5
66	17745.5
67	13633.0
68	10278.5
69	7689.5
70	5679.5
71	4137.0
72	3099.5
73	2208.0
74	1498.0
75	1058.0
76	723.5
77	489.0
78	342.5
79	239.5
80	172.0
81	130.0
82	98.5
83	74.0
84	58.5
85	50.0
86	48.5
87	35.5
88	22.0
89	20.5
90	19.0
91	17.0
92	15.0
93	12.0
94	8.0
95	5.0
96	4.0
97	4.0
98	6.0
99	12.0
100	18.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07775895231355776
2	0.012625357328786127
3	0.017067612685210874
4	0.010774417596942481
5	0.008787092832226147
6	0.0070335709810058515
7	0.019503059700794616
8	0.008787092832226147
9	0.009098830050220866
10-11	0.012352587263040747
12-13	0.014252235935196067
14-15	0.009137797202470205
16-17	0.012060333621170698
18-19	0.008446130250044422
20-21	0.012800709513908156
22-23	0.012566906600412117
24-25	0.01513873864886855
26-27	0.005211856613349212
28-29	0.011670662098677299
30-31	0.009839205942958323
32-33	0.011865497859923997
34-35	0.008475355614231427
36-37	0.010121717796766039
38-39	0.018246369040753406
40-41	0.012255169382417396
42-43	0.008056458727551024
44-45	0.019805055130727004
46-47	0.017184514141958894
48-49	0.017350124539018588
50-51	0.010189910313202382
52-53	0.010589323623758118
54-55	0.010589323623758118
56-57	0.01966867009785431
58-59	0.002221127678212374
60-61	0.00724789031837722
62-63	0.014242494147133732
64-65	0.02332184062122993
66-67	0.012566906600412117
68-69	0.013930756929139013
70-71	0.024656465585769817
72-73	0.01924977321117391
74-75	0.019590735793355633
76-77	0.009391083692090916
78-79	0.010998478722376186
80-81	0.019006228509615534
82-83	0.01151479348967994
84-85	0.010949769782064512
86-87	0.015849889177419002
88-89	0.014154818054572717
90-91	0.014661391033814135
92-93	0.007812914025992648
94-95	0.014856226795060835
96-97	0.012722775209409476
98-99	0.009605403029462285
100	0.00718943959000321
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5132528.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.26714298981607
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.39538231906074	52.734333921975576
2	14.186481892073873	19.08568211913139
3	3.704363061326181	7.475457591972631
4	1.4624997865704088	3.9351272906322863
5	0.6655215818001717	2.238386770288036
6	0.394655093437797	1.5928392361163706
7	0.2611402393498117	1.2296310474517003
8	0.176637759210753	0.950553392498434
9	0.12267599207586241	0.7426857150346122
>10	0.585628352244571	6.784719785262977
>50	0.031048971457109154	1.4415102659779995
>100	0.01367486117399995	1.5457982297613335
>500	2.0306357467772873E-4	0.0872646355428595
>1k	5.801776270105623E-5	0.05245214203569755
>5k	2.9008881350468475E-5	0.10355785631804265
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5307	0.10339933849362341	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.9483576124669947E-5	0.0
5	0.0	0.0	0.0	1.9483576124669947E-5	0.0
6	0.0	0.0	0.0	1.9483576124669947E-5	0.0
7	0.0	0.0	0.0	1.9483576124669947E-5	0.0
8	3.896715224933989E-5	0.0	0.0	1.9483576124669947E-5	0.0
9	3.896715224933989E-5	0.0	0.0	1.9483576124669947E-5	0.0
10-11	3.896715224933989E-5	0.0	0.0	1.9483576124669947E-5	0.0
12-13	3.896715224933989E-5	0.0	0.0	1.9483576124669947E-5	0.0
14-15	3.896715224933989E-5	9.741788062334973E-6	0.0	1.9483576124669947E-5	0.0
16-17	3.896715224933989E-5	1.9483576124669947E-5	0.0	1.9483576124669947E-5	0.0
18-19	3.896715224933989E-5	2.922536418700492E-5	9.741788062334973E-6	1.9483576124669947E-5	0.0
20-21	3.896715224933989E-5	3.896715224933989E-5	1.9483576124669947E-5	1.9483576124669947E-5	0.0
22-23	3.896715224933989E-5	3.896715224933989E-5	1.9483576124669947E-5	1.558686089973596E-4	0.0
24-25	5.845072837400985E-5	3.896715224933989E-5	1.9483576124669947E-5	3.1173721799471915E-4	0.0
26-27	5.845072837400985E-5	3.896715224933989E-5	1.9483576124669947E-5	4.383804628050738E-4	0.0
28-29	5.845072837400985E-5	3.896715224933989E-5	1.9483576124669947E-5	9.157280778594875E-4	0.0
30-31	5.845072837400985E-5	3.896715224933989E-5	1.9483576124669947E-5	0.0026984752932667877	0.0
32-33	5.845072837400985E-5	3.896715224933989E-5	1.9483576124669947E-5	0.006731575551073466	0.0
34-35	5.845072837400985E-5	5.845072837400985E-5	1.9483576124669947E-5	0.013628761499206629	0.0
36-37	5.845072837400985E-5	5.845072837400985E-5	1.9483576124669947E-5	0.02332184062122993	0.0
38-39	9.741788062334975E-5	7.79343044986798E-5	1.9483576124669947E-5	0.034446962588416466	0.0
40-41	9.741788062334975E-5	9.741788062334975E-5	1.9483576124669947E-5	0.046780066275332544	0.0
42-43	9.741788062334975E-5	9.741788062334975E-5	1.9483576124669947E-5	0.059337231087682324	0.0
44-45	9.741788062334975E-5	9.741788062334975E-5	1.9483576124669947E-5	0.07424216682305484	0.0
46-47	9.741788062334975E-5	9.741788062334975E-5	3.896715224933989E-5	0.09314123566398469	0.0
48-49	1.3638503287268964E-4	9.741788062334975E-5	3.896715224933989E-5	0.1129755161588987	0.0
50-51	1.3638503287268964E-4	9.741788062334975E-5	3.896715224933989E-5	0.13295592347474772	0.0
52-53	1.3638503287268964E-4	9.741788062334975E-5	3.896715224933989E-5	0.1522836309904203	0.0
54-55	1.3638503287268964E-4	9.741788062334975E-5	3.896715224933989E-5	0.1755080537310269	0.0
56-57	1.3638503287268964E-4	9.741788062334975E-5	3.896715224933989E-5	0.20297015427874918	0.0
58-59	1.3638503287268964E-4	9.741788062334975E-5	3.896715224933989E-5	0.23365678667510437	0.0
60-61	1.3638503287268964E-4	9.741788062334975E-5	3.896715224933989E-5	0.2648889592029503	0.0
62-63	1.3638503287268964E-4	9.741788062334975E-5	3.896715224933989E-5	0.29604319742629753	0.0
64-65	1.3638503287268964E-4	9.741788062334975E-5	3.896715224933989E-5	0.332467742991368	0.0
66-67	1.3638503287268964E-4	9.741788062334975E-5	3.896715224933989E-5	0.3749516807312108	0.0
68-69	1.5586860899735957E-4	9.741788062334975E-5	3.896715224933989E-5	0.42362165389063633	9.741788062334973E-6
70-71	1.5586860899735957E-4	9.741788062334975E-5	3.896715224933989E-5	0.47568176929575445	1.9483576124669947E-5
72-73	1.5586860899735957E-4	1.2664324481035467E-4	3.896715224933989E-5	0.5325348444275413	1.9483576124669947E-5
74-75	1.5586860899735957E-4	1.461268209350246E-4	3.896715224933989E-5	0.5914142114762939	1.9483576124669947E-5
76-77	1.5586860899735957E-4	1.7535218512202953E-4	3.896715224933989E-5	0.6578921732136678	1.9483576124669947E-5
78-79	1.5586860899735957E-4	1.7535218512202953E-4	3.896715224933989E-5	0.7321830489770342	1.9483576124669947E-5
80-81	1.5586860899735957E-4	1.7535218512202953E-4	3.896715224933989E-5	0.8159624263131151	1.9483576124669947E-5
82-83	1.5586860899735957E-4	1.7535218512202953E-4	3.896715224933989E-5	0.9064538956241446	1.9483576124669947E-5
84-85	1.5586860899735957E-4	1.7535218512202953E-4	3.896715224933989E-5	0.9986209524818959	1.9483576124669947E-5
86-87	1.5586860899735957E-4	1.7535218512202953E-4	3.896715224933989E-5	1.1005687645542315	1.9483576124669947E-5
88	1.5586860899735957E-4	1.7535218512202953E-4	3.896715224933989E-5	1.1861406308937819	1.9483576124669947E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5740	0.0	47.595524	1
GTATCAA	9765	0.0	35.344814	1
ATCAACG	10515	0.0	31.466259	3
TCAACGC	10575	0.0	31.287727	4
CAACGCA	11270	0.0	29.401121	5
TATCAAC	11810	0.0	28.294186	2
AACGCAG	11850	0.0	27.804783	6
ACGCAGA	13645	0.0	24.009275	7
CGCAGAG	13670	0.0	23.62038	8
GTACATG	15725	0.0	23.11483	1
TACATGG	15630	0.0	22.281105	2
GTACCTG	3595	0.0	21.97416	1
ACATGGG	15960	0.0	21.113878	3
TACCTGG	3690	0.0	21.015274	2
GTGGTAT	3360	0.0	20.432215	1
TGGTATC	3355	0.0	19.611599	2
GCAGAGT	16095	0.0	19.243525	9
CAGAGTA	15195	0.0	17.661764	10-11
ATAACGC	455	0.0	17.559788	3
AGAGTAC	14005	0.0	17.266365	10-11
>>END_MODULE