FastQCFastQC Report
Wed 25 May 2016
SRR2049445_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049445_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5132528
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208810.4068365530592332No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199140.38799593494667733No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118640.23115314714308427No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54860.10688689861993933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102100.057.6640321
GTATCAA186600.041.2362821
GTGGTAT40650.035.6585431
ATCAACG232850.032.5600743
TGGTATC43000.032.2463722
TCAACGC235300.032.2410244
TATCAAC242150.031.523092
CAACGCA244300.031.0725065
AACGCAG253100.030.0635056
ACGCAGA284500.026.6463097
CGCAGAG288800.026.1844718
GCAGAGT316950.023.651299
GTACATG145750.022.6351241
GTACCTG33700.022.6234341
TACATGG145600.021.6614932
TATAACG5050.021.4073892
CAGAGTA311600.021.06339810-11
GAGTACT238450.020.86308312-13
TACCTGG35250.020.8014092
AGAGTAC298150.020.76829110-11
Produced by FastQC (version 0.11.5)