##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049445_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5132528
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.965470621884577	33.0	31.0	34.0	30.0	34.0
2	32.196532001384114	33.0	31.0	34.0	30.0	34.0
3	32.20396829788361	34.0	31.0	34.0	30.0	34.0
4	35.88280141871608	37.0	35.0	37.0	35.0	37.0
5	35.922477188629074	37.0	35.0	37.0	35.0	37.0
6	35.978179174083415	37.0	35.0	37.0	35.0	37.0
7	35.94896082398382	37.0	35.0	37.0	35.0	37.0
8	35.960816385219914	37.0	35.0	37.0	35.0	37.0
9	37.69199544551925	39.0	37.0	39.0	35.0	39.0
10-11	37.664032129975716	39.0	37.0	39.0	35.0	39.0
12-13	37.64290569871221	39.0	37.0	39.0	35.0	39.0
14-15	38.95080465221037	40.0	38.0	41.0	36.0	41.0
16-17	38.959970213508825	40.0	38.0	41.0	36.0	41.0
18-19	39.04323863406103	40.0	38.5	41.0	36.0	41.0
20-21	39.066467732859905	40.0	39.0	41.0	36.0	41.0
22-23	39.02571646954483	40.0	39.0	41.0	35.0	41.0
24-25	39.00038363161389	40.0	39.0	41.0	35.0	41.0
26-27	38.89807800366603	40.0	38.0	41.0	35.0	41.0
28-29	38.81501523225981	40.0	38.0	41.0	35.0	41.0
30-31	38.690633251294486	40.0	38.0	41.0	35.0	41.0
32-33	38.53516717297987	40.0	38.0	41.0	35.0	41.0
34-35	38.40944121493346	40.0	38.0	41.0	34.0	41.0
36-37	38.271347180180996	40.0	38.0	41.0	34.0	41.0
38-39	38.16543153783087	40.0	38.0	41.0	34.0	41.0
40-41	38.07086322763364	40.0	38.0	41.0	34.0	41.0
42-43	37.92634575008651	40.0	38.0	41.0	33.0	41.0
44-45	37.740443013657206	40.0	38.0	41.0	33.0	41.0
46-47	37.47264759198586	40.0	37.0	41.0	33.0	41.0
48-49	37.21024541902158	40.0	37.0	41.0	32.0	41.0
50-51	37.053186850612406	40.0	37.0	41.0	32.0	41.0
52-53	36.94654632181256	40.0	37.0	41.0	31.0	41.0
54-55	36.81151841743484	40.0	36.5	41.0	31.0	41.0
56-57	36.673016201762564	40.0	36.0	41.0	31.0	41.0
58-59	36.50682383028402	39.5	36.0	41.0	31.0	41.0
60-61	36.32828422952588	39.0	35.0	41.0	31.0	41.0
62-63	36.11272914633881	39.0	35.0	41.0	30.5	41.0
64-65	35.895321175062264	39.0	35.0	41.0	30.0	41.0
66-67	35.64078919783779	38.0	35.0	40.0	30.0	41.0
68-69	35.35191439773928	37.5	35.0	40.0	29.0	41.0
70-71	35.06819553639065	37.0	35.0	40.0	29.0	41.0
72-73	34.73634045445051	36.5	34.5	39.0	29.0	41.0
74-75	34.412656004994034	36.0	34.0	39.0	29.0	41.0
76-77	34.09028874270145	36.0	34.0	39.0	29.0	40.0
78-79	33.775638145568806	35.0	34.0	37.5	29.0	39.5
80-81	33.459035294108475	35.0	34.0	37.0	28.5	39.0
82-83	33.173885266675605	35.0	34.0	37.0	28.0	39.0
84-85	32.87979013460813	35.0	34.0	36.0	28.0	38.0
86-87	32.62442026619241	35.0	34.0	36.0	27.0	37.0
88-89	32.39521489215451	35.0	34.0	36.0	27.0	37.0
90-91	32.195159091192494	35.0	33.5	35.0	27.0	36.5
92-93	32.02581398484334	35.0	33.0	35.0	26.5	36.0
94-95	31.860858235941432	35.0	33.0	35.0	26.0	36.0
96-97	31.70950406895004	35.0	33.0	35.0	26.0	36.0
98-99	31.57350822051044	35.0	33.0	35.0	25.5	36.0
100	31.469212442679318	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.35378394488263964
1101	2	0.3725650076556519
1101	3	0.47174220660475186
1101	4	0.2676511842031246
1101	5	0.2360928431548288
1101	6	0.2403375007103392
1101	7	0.24402226700181728
1101	8	0.24169617253892994
1101	9	0.3077846167374716
1101	10-11	0.27313561875698866
1101	12-13	0.2697077796504672
1101	14-15	0.3662273703637311
1101	16-17	0.37449555120947053
1101	18-19	0.36420697414926195
1101	20-21	0.38598779101130987
1101	22-23	0.3987828293949889
1101	24-25	0.3948007403276179
1101	26-27	0.3593609746924571
1101	28-29	0.3883874665003759
1101	30-31	0.39100538235343407
1101	32-33	0.42015540108819494
1101	34-35	0.41343721514412124
1101	36-37	0.4468649419212056
1101	38-39	0.45238629484872916
1101	40-41	0.4037096171516126
1101	42-43	0.3701917936060539
1101	44-45	0.38659616264541796
1101	46-47	0.35386603279891204
1101	48-49	0.30675265806626584
1101	50-51	0.32008522427912567
1101	52-53	0.35620127561298887
1101	54-55	0.31626150052495206
1101	56-57	0.3307333511585284
1101	58-59	0.3410895518009198
1101	60-61	0.25343323117787975
1101	62-63	0.2224202345146864
1101	64-65	0.24164362644203408
1101	66-67	0.2572543299760284
1101	68-69	0.3259419612453769
1101	70-71	0.3229638039637521
1101	72-73	0.3484526158180188
1101	74-75	0.3170081864268397
1101	76-77	0.3052880687329207
1101	78-79	0.26779864491015815
1101	80-81	0.2352212996106644
1101	82-83	0.1989892596594558
1101	84-85	0.192487790480115
1101	86-87	0.19725786285411573
1101	88-89	0.2513198818891098
1101	90-91	0.23597752478084288
1101	92-93	0.21252609286136703
1101	94-95	0.21760966886850142
1101	96-97	0.20258460611196583
1101	98-99	0.23758522302419394
1101	100	0.20526392547969508
1102	1	0.28243730334066797
1102	2	0.2846624452934421
1102	3	0.3182653163269862
1102	4	0.2249058899662444
1102	5	0.21102358298418267
1102	6	0.17045050571040576
1102	7	0.17428250763634168
1102	8	0.16964463214387138
1102	9	0.1853544727832741
1102	10-11	0.23104698389541767
1102	12-13	0.22530431010546437
1102	14-15	0.30775530485905733
1102	16-17	0.26832656794745446
1102	18-19	0.28244473196842534
1102	20-21	0.2598689157330938
1102	22-23	0.25827856220259093
1102	24-25	0.30881496474245296
1102	26-27	0.2579696392124333
1102	28-29	0.277332306947784
1102	30-31	0.2881235336680916
1102	32-33	0.32732085762761187
1102	34-35	0.3237439736558869
1102	36-37	0.3004506990854168
1102	38-39	0.2959824360866605
1102	40-41	0.3338925673311621
1102	42-43	0.24607628031819928
1102	44-45	0.25997227904476006
1102	46-47	0.2667393873746633
1102	48-49	0.3173329496852517
1102	50-51	0.3025624918894607
1102	52-53	0.2892296947314321
1102	54-55	0.32256700687359086
1102	56-57	0.3447878755511127
1102	58-59	0.387104855393531
1102	60-61	0.32438496099850767
1102	62-63	0.31227292625278835
1102	64-65	0.2535579108735462
1102	66-67	0.2640619147900267
1102	68-69	0.2506211130096645
1102	70-71	0.23670183776719966
1102	72-73	0.24719196846981362
1102	74-75	0.21790531691656412
1102	76-77	0.22730067916789665
1102	78-79	0.1775299547328899
1102	80-81	0.20478214787279114
1102	82-83	0.2267669870015112
1102	84-85	0.15360025143461087
1102	86-87	0.13758307038703776
1102	88-89	0.1649331285404898
1102	90-91	0.17092339092582876
1102	92-93	0.14611291797911008
1102	94-95	0.14394443747416474
1102	96-97	0.14754905662923434
1102	98-99	0.12582970383370906
1102	100	0.13241513383528414
1103	1	0.3010832976787192
1103	2	0.3194205015217122
1103	3	0.38188128574307
1103	4	0.2019982416030004
1103	5	0.23940393818108419
1103	6	0.2410476108655928
1103	7	0.21605264092556098
1103	8	0.213095680367978
1103	9	0.25174896500697486
1103	10-11	0.2876980676862573
1103	12-13	0.27500663998700503
1103	14-15	0.3157416487645932
1103	16-17	0.37062648099602313
1103	18-19	0.32439161361203617
1103	20-21	0.3717595039039452
1103	22-23	0.3269552433069549
1103	24-25	0.3344995375414612
1103	26-27	0.3454452847612757
1103	28-29	0.3835567754240259
1103	30-31	0.39875646958434885
1103	32-33	0.4155007043765053
1103	34-35	0.4461559333608207
1103	36-37	0.416897229166139
1103	38-39	0.43983343641485817
1103	40-41	0.3958957499460567
1103	42-43	0.4195675672577579
1103	44-45	0.37064871632836116
1103	46-47	0.3967058148564959
1103	48-49	0.41372109696468584
1103	50-51	0.42445226745595477
1103	52-53	0.43489616922513363
1103	54-55	0.3837182375567707
1103	56-57	0.39064300330957025
1103	58-59	0.38857118397363877
1103	60-61	0.32687232359970153
1103	62-63	0.3716048644377139
1103	64-65	0.3412348822106708
1103	66-67	0.3134887031234399
1103	68-69	0.3575910048934219
1103	70-71	0.33641633500781865
1103	72-73	0.3844768374846197
1103	74-75	0.3940514205753587
1103	76-77	0.3867456419759492
1103	78-79	0.3404441343197959
1103	80-81	0.31577833845933867
1103	82-83	0.31415491942819074
1103	84-85	0.3910176708620696
1103	86-87	0.327833315825913
1103	88-89	0.2953270908940624
1103	90-91	0.28407973174196144
1103	92-93	0.2888284231017195
1103	94-95	0.2586621606633095
1103	96-97	0.281239256407563
1103	98-99	0.3668172446632276
1103	100	0.36158459929711384
1104	1	0.26013636434720055
1104	2	0.2717321252131839
1104	3	0.3387996453547828
1104	4	0.19233848743277093
1104	5	0.15821766240296142
1104	6	0.1532311600773184
1104	7	0.1793982150591944
1104	8	0.19802130124057982
1104	9	0.22861593292745397
1104	10-11	0.2199061722611262
1104	12-13	0.17489610964563695
1104	14-15	0.2851267774026951
1104	16-17	0.30121431554701417
1104	18-19	0.2532738594584458
1104	20-21	0.2700190954939572
1104	22-23	0.26631405952019804
1104	24-25	0.2910138107836673
1104	26-27	0.27551789737021437
1104	28-29	0.3217218677331246
1104	30-31	0.32178679204395166
1104	32-33	0.3361228309469837
1104	34-35	0.3244261770193191
1104	36-37	0.33177963653361786
1104	38-39	0.3948198319530931
1104	40-41	0.38032289675773967
1104	42-43	0.33030701599808054
1104	44-45	0.3028859003693185
1104	46-47	0.31358625106172866
1104	48-49	0.27811891276106593
1104	50-51	0.29506869161405547
1104	52-53	0.2933249399476807
1104	54-55	0.27416304652690116
1104	56-57	0.27230881130451934
1104	58-59	0.35912166630505027
1104	60-61	0.2238624239092033
1104	62-63	0.1922358992543849
1104	64-65	0.18855130614181093
1104	66-67	0.22709083853029455
1104	68-69	0.22376142928908394
1104	70-71	0.23356622580141817
1104	72-73	0.19900258401835202
1104	74-75	0.22112177453724513
1104	76-77	0.15438778997160796
1104	78-79	0.1694026298361635
1104	80-81	0.19093222793958375
1104	82-83	0.16828200965075268
1104	84-85	0.1496396710905401
1104	86-87	0.13016178196588157
1104	88-89	0.1557966349053359
1104	90-91	0.13490618435348978
1104	92-93	0.16788508900427246
1104	94-95	0.1503888019556605
1104	96-97	0.1363134577483045
1104	98-99	0.18877980695475927
1104	100	0.2145331342215968
1105	1	0.18514043689083692
1105	2	0.1950876902713432
1105	3	0.3085418097081032
1105	4	0.1728355850181913
1105	5	0.18499209148325235
1105	6	0.14490051753174527
1105	7	0.15643975936773558
1105	8	0.12618679486923412
1105	9	0.1701677320463304
1105	10-11	0.11572441536579703
1105	12-13	0.1536428861546213
1105	14-15	0.1964573028626404
1105	16-17	0.21541072996323152
1105	18-19	0.17723352454991215
1105	20-21	0.16019184770440376
1105	22-23	0.12025037293733476
1105	24-25	0.14459448569141387
1105	26-27	0.15678339667133656
1105	28-29	0.21102622273674854
1105	30-31	0.17919909418014157
1105	32-33	0.15816265533308638
1105	34-35	0.19573199813866182
1105	36-37	0.23208456823851975
1105	38-39	0.21126027245686885
1105	40-41	0.25502805550694774
1105	42-43	0.21233284465163393
1105	44-45	0.28599720735039114
1105	46-47	0.32998960232880137
1105	48-49	0.3145962679639567
1105	50-51	0.24195781370455194
1105	52-53	0.2331521986458327
1105	54-55	0.25930620734437326
1105	56-57	0.2023120402498364
1105	58-59	0.23110360239229522
1105	60-61	0.22205499631684944
1105	62-63	0.24245361671003707
1105	64-65	0.1982442872582979
1105	66-67	0.17331075443227206
1105	68-69	0.2088822638405432
1105	70-71	0.20371891709825007
1105	72-73	0.25518290534204624
1105	74-75	0.2813903842435721
1105	76-77	0.2699531972041598
1105	78-79	0.2470090214025049
1105	80-81	0.23444741125496193
1105	82-83	0.21196313093837915
1105	84-85	0.2126624838800737
1105	86-87	0.1722082315077884
1105	88-89	0.1471252259070539
1105	90-91	0.2180228910910884
1105	92-93	0.2346740956090656
1105	94-95	0.2075332070806688
1105	96-97	0.26951839687104595
1105	98-99	0.298251616225901
1105	100	0.21023376444772524
1106	1	0.2142723739853416
1106	2	0.19801003924442284
1106	3	0.2587923306953499
1106	4	0.13785933421216612
1106	5	0.1491496360701916
1106	6	0.13955382261335103
1106	7	0.10150569752552485
1106	8	0.11570986885815415
1106	9	0.14610657446611697
1106	10-11	0.15651120216517
1106	12-13	0.20418486658380175
1106	14-15	0.2423150438284125
1106	16-17	0.27271941429491164
1106	18-19	0.2673949170721457
1106	20-21	0.23913348385934796
1106	22-23	0.24159427551778379
1106	24-25	0.17918557619823616
1106	26-27	0.16336910859175902
1106	28-29	0.18636785973002645
1106	30-31	0.21270854670166983
1106	32-33	0.16845308782061608
1106	34-35	0.12919630308962127
1106	36-37	0.1572195917851147
1106	38-39	0.1908564697913988
1106	40-41	0.19524318848356614
1106	42-43	0.19239986914553953
1106	44-45	0.2800394221627016
1106	46-47	0.20793944154406319
1106	48-49	0.2269318941301961
1106	50-51	0.26015410101985026
1106	52-53	0.24317111696244353
1106	54-55	0.29600106984775465
1106	56-57	0.328533801623351
1106	58-59	0.270639565706972
1106	60-61	0.2837162382889602
1106	62-63	0.27342808117427353
1106	64-65	0.2756775055308296
1106	66-67	0.27666056444570586
1106	68-69	0.31693889583527834
1106	70-71	0.32901715304206647
1106	72-73	0.36399817370898546
1106	74-75	0.317020724668474
1106	76-77	0.3135805957889204
1106	78-79	0.2632862837236871
1106	80-81	0.1933681171009809
1106	82-83	0.2072164358802695
1106	84-85	0.22896769053893706
1106	86-87	0.2397520534688269
1106	88-89	0.2609464778552919
1106	90-91	0.2840881257501664
1106	92-93	0.26174131291890745
1106	94-95	0.23239689110844708
1106	96-97	0.23272927462365445
1106	98-99	0.2888943674297799
1106	100	0.22083664956086935
1107	1	0.08811035983876181
1107	2	0.11194988076320556
1107	3	0.19586834068005743
1107	4	0.10963129546630057
1107	5	0.15578638377639464
1107	6	0.10902184131406045
1107	7	0.06464981913283907
1107	8	0.11854084228614425
1107	9	0.09397674213808216
1107	10-11	0.1171406500237353
1107	12-13	0.11363084507379995
1107	14-15	0.15281352860618824
1107	16-17	0.18131095420838506
1107	18-19	0.15061788028007328
1107	20-21	0.16909193951618562
1107	22-23	0.16826835767724901
1107	24-25	0.1424150960137922
1107	26-27	0.17815312602374433
1107	28-29	0.20139003181679982
1107	30-31	0.18397055334146017
1107	32-33	0.13063309392072142
1107	34-35	0.14749324539079822
1107	36-37	0.15313701301959526
1107	38-39	0.11952490697882467
1107	40-41	0.09823243010148985
1107	42-43	0.1011896120277811
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2216	72-73	-0.6841989720198498
2216	74-75	-0.6807353657780482
2216	76-77	-0.5507473752783199
2216	78-79	-0.5823722617871994
2216	80-81	-0.6368529611412512
2216	82-83	-0.6123540420386746
2216	84-85	-0.7054480292479894
2216	86-87	-0.6685758439047405
2216	88-89	-0.6452876783534549
2216	90-91	-0.6224599921608487
2216	92-93	-0.6493856413996966
2216	94-95	-0.6780192063936141
2216	96-97	-0.7606197795613774
2216	98-99	-0.8272114637018504
2216	100	-0.8466859986369819
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	396.0
3	44.0
4	52.0
5	127.0
6	435.0
7	1195.0
8	2541.0
9	4062.0
10	5100.0
11	6207.0
12	9388.0
13	13745.0
14	15305.0
15	19119.0
16	24414.0
17	34769.0
18	36355.0
19	19257.0
20	13372.0
21	12295.0
22	12484.0
23	13440.0
24	15541.0
25	18875.0
26	24191.0
27	30852.0
28	40561.0
29	52325.0
30	68255.0
31	88531.0
32	115667.0
33	151637.0
34	211049.0
35	313846.0
36	503691.0
37	993669.0
38	1556936.0
39	700375.0
40	2425.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.728231621180875	20.810817717987153	15.029574393661038	22.43137626717093
2	15.431713183055212	21.37942549948096	40.11321516414523	23.075646153318598
3	17.432793352515564	26.503976208215523	31.66496120430322	24.398269234965696
4	12.360146890577118	18.340611098468436	39.06490135075737	30.234340660197077
5	12.001472391586011	37.77198858799089	35.87960685040124	14.346932170021864
6	28.649262856657437	39.078241548589425	18.255257265637358	14.017238329115777
7	24.853171770324487	33.68953856656993	23.521995398758662	17.935294264346926
8	24.070925672495115	35.756492706907785	21.425523640591926	18.747057980005174
9	24.31480159484761	16.888324817711663	22.79636857314758	36.00050501429315
10-11	22.832539832223027	27.42153574223073	29.528489664352538	20.217434761193704
12-13	23.91432642939308	25.34123535224747	29.72693962897036	21.01749858938909
14-15	21.265349161271015	27.17963740285489	27.936398983113197	23.6186144527609
16-17	19.79834303875205	30.32473471162749	28.460848143448995	21.41607410617146
18-19	19.56876806127507	28.314516744964664	31.95872482332293	20.157990370437336
20-21	20.783237811854118	27.646424919649732	31.764220282870355	19.806116985625795
22-23	20.441223116561662	27.603307765685837	31.832860921557565	20.122608196194935
24-25	20.16430304910173	27.704222948223567	31.85349402867359	20.277979974001116
26-27	20.080669798586584	27.524652568870543	31.934886667934396	20.459790964608473
28-29	20.06634937013495	27.539216542023738	32.05806183619456	20.336372251646754
30-31	20.44313250702188	27.948381382429865	31.599515872100454	20.008970238447798
32-33	19.944615986508012	27.709902410663904	31.98751180704713	20.357969795780946
34-35	20.175545072525665	27.44181814497651	31.89643583045236	20.486200952045465
36-37	20.651801607317097	27.783803614904777	31.29630271866028	20.268092059117844
38-39	20.45497852130568	27.87959461692172	31.39811414569974	20.267312716072862
40-41	20.260659074826286	27.725830234145825	31.476779084303097	20.536731606724796
42-43	20.2958561550955	27.797763597198106	31.21140303569703	20.694977212009363
44-45	20.571996879510447	27.97884395370079	30.902637063061324	20.54652210372744
46-47	20.67094422085958	28.229519644120792	30.398908685934106	20.700627449085516
48-49	20.686540823547382	28.10682182347568	30.301549256039127	20.905088096937803
50-51	20.81560977358526	28.118404809481802	30.00871110688534	21.057274310047603
52-53	20.801951786721865	28.226977037436523	29.887581714118266	21.083489461723346
54-55	20.944522855014135	28.61184585841519	29.672707094827345	20.77092419174333
56-57	20.759536041498457	28.302047256244876	29.737714046567305	21.20070265568936
58-59	20.61305851619319	28.003870607232926	30.073601157168557	21.30946971940533
60-61	20.923753362865238	28.33564668327187	29.748790459594183	20.99180949426871
62-63	20.773486282003724	28.27844290376984	29.89267666927487	21.055394144951574
64-65	20.73375927028552	28.056271685220224	30.06631429969793	21.143654744796326
66-67	20.709667828407365	28.14528240274578	29.82929659614132	21.315753172705538
68-69	21.112558957301353	28.274507221392653	29.673993010851575	20.93894081045442
70-71	21.145525168104296	28.05436229476001	29.72892695373508	21.071185583400617
72-73	20.81803547881278	28.277410274235233	29.882642627570664	21.021911619381324
74-75	20.925255636720895	28.226765467890818	29.68868651945849	21.15929237592979
76-77	21.025457630236016	28.30233950988675	29.478368164771823	21.193834695105416
78-79	21.136017182955456	28.382767712129382	29.54073509194689	20.94048001296827
80-81	20.9432054708419	28.1878352520468	29.689573482338787	21.17938579477251
82-83	20.86188788486236	28.28389398111116	29.745043376030843	21.10917475799564
84-85	20.901280094674906	28.21855618039591	29.634237510645374	21.24592621428381
86-87	21.00885435084817	28.382661098235655	29.46924552435896	21.139239026557206
88-89	20.85616213159383	28.23595140572402	29.55046738941422	21.357419073267934
90-91	20.708945718305202	28.128729607016627	29.671478459450856	21.490846215227315
92-93	20.853618215000555	28.411362300407383	29.455664735255628	21.279354749336438
94-95	20.713570317239242	28.18559986532917	29.628606017576764	21.472223799854827
96-97	20.674242900264442	28.282225174001546	29.60035538254037	21.44317654319364
98-99	20.616785081096943	28.29512738363616	29.515397902588198	21.572689632678703
100	20.754962180386173	28.239411345923116	29.444257618379428	21.56136885531129
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2150.0
1	1565.5
2	2538.0
3	3798.0
4	2198.0
5	1056.0
6	2321.0
7	5839.5
8	9580.0
9	10907.5
10	8094.5
11	4473.5
12	3749.0
13	4028.5
14	4536.0
15	5155.0
16	5783.0
17	6579.5
18	7667.0
19	8749.0
20	10238.5
21	12596.5
22	15996.0
23	21262.0
24	28498.0
25	37363.0
26	48597.0
27	62356.5
28	77809.5
29	94425.0
30	109365.5
31	122225.0
32	135461.0
33	147420.0
34	156996.5
35	166071.5
36	173708.0
37	179581.0
38	185100.5
39	187723.5
40	187335.5
41	185377.5
42	181899.0
43	178315.0
44	172819.0
45	167529.0
46	163541.5
47	157914.0
48	152944.5
49	148570.0
50	144459.0
51	137634.5
52	126182.0
53	116129.5
54	108998.5
55	102800.5
56	95580.0
57	86385.0
58	78033.0
59	70924.5
60	61753.0
61	50828.5
62	41504.5
63	33812.5
64	26645.5
65	20875.5
66	16441.0
67	12684.0
68	9473.5
69	6838.0
70	4962.5
71	3516.5
72	2459.5
73	1704.0
74	1146.0
75	792.0
76	505.0
77	313.5
78	188.0
79	103.0
80	58.0
81	25.5
82	12.5
83	7.0
84	3.5
85	2.5
86	3.0
87	2.5
88	1.0
89	0.0
90	0.0
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	1.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12939042904393314
2	0.0
3	0.0
4	0.0
5	0.014437329908380432
6	2.338029134960394E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	9.741788062334973E-6
76-77	0.0
78-79	0.0
80-81	1.7535218512202953E-4
82-83	5.357983434284236E-4
84-85	3.312207941193891E-4
86-87	4.481222508674088E-4
88-89	5.942490718024334E-4
90-91	6.526998001764433E-4
92-93	2.1431933737136943E-4
94-95	2.630282776830443E-4
96-97	5.942490718024334E-4
98-99	0.0011592727794178619
100	1.169014567480197E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5132528.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.42316223520976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.25823736196064	49.77219959575934
2	14.728094496455002	18.97660842321241
3	4.00395953163838	7.738432034698614
4	1.5560129289515348	4.009730934477145
5	0.7539735664484005	2.4286680696182508
6	0.43974101231798607	1.6997703946822194
7	0.25748734406075596	1.161170425795755
8	0.1747729408651318	0.9007540418943284
9	0.13269047558232216	0.7693506031956371
>10	0.6421501410290712	7.1914333326073745
>50	0.0341344509466342	1.5027353368515723
>100	0.0176868453084321	1.9787316535259951
>500	6.050896671814425E-4	0.2667130917217563
>1k	3.3279749696680974E-4	0.470410175807595
>5k	3.0254317905957766E-5	0.10692646465610411
>10k+	9.07629537178733E-5	1.0263654214957685
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20881	0.4068365530592332	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19914	0.38799593494667733	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11864	0.23115314714308427	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5486	0.10688689861993933	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.9483576124669947E-5	0.0	0.0	0.0	0.0
7	1.9483576124669947E-5	0.0	0.0	0.0	0.0
8	1.9483576124669947E-5	0.0	0.0	0.0	0.0
9	1.9483576124669947E-5	0.0	0.0	0.0	0.0
10-11	1.9483576124669947E-5	0.0	0.0	9.741788062334973E-6	0.0
12-13	1.9483576124669947E-5	0.0	9.741788062334973E-6	1.9483576124669947E-5	0.0
14-15	1.9483576124669947E-5	9.741788062334973E-6	1.9483576124669947E-5	1.9483576124669947E-5	0.0
16-17	1.9483576124669947E-5	2.922536418700492E-5	1.9483576124669947E-5	2.922536418700492E-5	0.0
18-19	1.9483576124669947E-5	4.870894031167487E-5	1.9483576124669947E-5	3.896715224933989E-5	0.0
20-21	1.9483576124669947E-5	5.845072837400985E-5	1.9483576124669947E-5	3.896715224933989E-5	0.0
22-23	2.922536418700492E-5	5.845072837400985E-5	1.9483576124669947E-5	1.558686089973596E-4	0.0
24-25	3.896715224933989E-5	9.741788062334975E-5	1.9483576124669947E-5	2.922536418700492E-4	0.0
26-27	3.896715224933989E-5	9.741788062334975E-5	1.9483576124669947E-5	4.091550986180689E-4	0.0
28-29	3.896715224933989E-5	9.741788062334975E-5	1.9483576124669947E-5	9.059862897971525E-4	0.0
30-31	3.896715224933989E-5	9.741788062334975E-5	1.9483576124669947E-5	0.0026789917171421177	0.0
32-33	3.896715224933989E-5	9.741788062334975E-5	1.9483576124669947E-5	0.006673124822699457	0.0
34-35	5.845072837400984E-5	9.741788062334975E-5	1.9483576124669947E-5	0.013570310770832619	0.0
36-37	7.793430449867979E-5	1.169014567480197E-4	1.9483576124669947E-5	0.023204939164481908	0.0
38-39	1.3638503287268964E-4	1.3638503287268964E-4	1.9483576124669947E-5	0.03432031934360612	0.0
40-41	1.3638503287268964E-4	1.3638503287268964E-4	1.9483576124669947E-5	0.046692390182771534	0.0
42-43	1.3638503287268964E-4	1.3638503287268964E-4	2.922536418700492E-5	0.0591911042667473	0.0
44-45	1.3638503287268964E-4	1.3638503287268964E-4	3.896715224933989E-5	0.07415449073049382	0.0
46-47	1.3638503287268964E-4	1.3638503287268964E-4	3.896715224933989E-5	0.09307304314754834	0.0
48-49	1.5586860899735957E-4	1.3638503287268964E-4	3.896715224933989E-5	0.11299499973502336	0.0
50-51	1.5586860899735957E-4	1.3638503287268964E-4	3.896715224933989E-5	0.13298514883893475	0.0
52-53	1.5586860899735957E-4	1.7535218512202953E-4	3.896715224933989E-5	0.15225440562623332	0.0
54-55	1.6561039705969454E-4	1.7535218512202953E-4	3.896715224933989E-5	0.17540089406234122	0.0
56-57	1.7535218512202953E-4	1.7535218512202953E-4	3.896715224933989E-5	0.20271686778912845	0.0
58-59	1.7535218512202953E-4	1.7535218512202953E-4	3.896715224933989E-5	0.23334504945710963	0.0
60-61	1.7535218512202953E-4	1.7535218512202953E-4	3.896715224933989E-5	0.26483050847457623	0.0
62-63	1.7535218512202953E-4	1.7535218512202953E-4	3.896715224933989E-5	0.2962185496114196	0.0
64-65	1.7535218512202953E-4	1.7535218512202953E-4	3.896715224933989E-5	0.33262361160036535	0.0
66-67	1.7535218512202953E-4	1.7535218512202953E-4	3.896715224933989E-5	0.3749516807312108	0.0
68-69	1.7535218512202953E-4	1.7535218512202953E-4	3.896715224933989E-5	0.42340733455326496	0.0
70-71	1.7535218512202953E-4	1.7535218512202953E-4	5.845072837400985E-5	0.4756428021435051	0.0
72-73	1.7535218512202953E-4	1.7535218512202953E-4	5.845072837400985E-5	0.5323984593946687	0.0
74-75	1.7535218512202953E-4	1.7535218512202953E-4	5.845072837400985E-5	0.5913947279001692	0.0
76-77	1.7535218512202953E-4	1.8509397318436453E-4	5.845072837400985E-5	0.6580577836107275	0.0
78-79	1.7535218512202953E-4	1.948357612466995E-4	5.845072837400985E-5	0.7324071101024681	0.0
80-81	1.7535218512202953E-4	1.948357612466995E-4	5.845072837400985E-5	0.8163910649878579	0.0
82-83	1.7535218512202953E-4	1.948357612466995E-4	5.845072837400985E-5	0.9068630507227627	0.0
84-85	1.7535218512202953E-4	1.948357612466995E-4	5.845072837400985E-5	0.9993418447985085	0.0
86-87	1.7535218512202953E-4	1.948357612466995E-4	5.845072837400985E-5	1.1017864880620232	0.0
88	1.7535218512202953E-4	1.948357612466995E-4	5.845072837400985E-5	1.1874460304941348	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10210	0.0	57.664032	1
GTATCAA	18660	0.0	41.236282	1
GTGGTAT	4065	0.0	35.658543	1
ATCAACG	23285	0.0	32.560074	3
TGGTATC	4300	0.0	32.246372	2
TCAACGC	23530	0.0	32.241024	4
TATCAAC	24215	0.0	31.52309	2
CAACGCA	24430	0.0	31.072506	5
AACGCAG	25310	0.0	30.063505	6
ACGCAGA	28450	0.0	26.646309	7
CGCAGAG	28880	0.0	26.184471	8
GCAGAGT	31695	0.0	23.65129	9
GTACATG	14575	0.0	22.635124	1
GTACCTG	3370	0.0	22.623434	1
TACATGG	14560	0.0	21.661493	2
TATAACG	505	0.0	21.407389	2
CAGAGTA	31160	0.0	21.063398	10-11
GAGTACT	23845	0.0	20.863083	12-13
TACCTGG	3525	0.0	20.801409	2
AGAGTAC	29815	0.0	20.768291	10-11
>>END_MODULE