FastQCFastQC Report
Wed 25 May 2016
SRR2049444_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049444_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4179704
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43850.10491173537647643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46600.043.695581
GTATCAA76700.034.7021641
TCAACGC80950.032.106984
ATCAACG81300.031.970673
CAACGCA89350.029.1418295
TATCAAC91850.028.6566892
AACGCAG95500.027.4141126
GTACCTG30900.025.8717581
ACGCAGA106650.024.416117
CGCAGAG105650.024.2436168
TACCTGG32300.024.0103552
GTACATG119550.023.8767171
TACATGG118700.023.2832582
ACATGGG121950.021.8148273
TCTGTCG11850.021.0197668
GCAGAGT120550.020.1567129
GTCGTAC2601.6754711E-819.8954981
GTGGTAT27250.019.3280051
CATGGGG71250.018.9976024
TAGACCG1851.6508874E-417.7839365
Produced by FastQC (version 0.11.5)