##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049444_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4179704
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.174892049771945	31.0	31.0	34.0	27.0	34.0
2	30.338023218869086	31.0	31.0	34.0	27.0	34.0
3	30.403119694600385	31.0	31.0	34.0	27.0	34.0
4	34.10315347689693	37.0	35.0	37.0	30.0	37.0
5	34.12919934043176	37.0	35.0	37.0	30.0	37.0
6	34.27370933444091	37.0	35.0	37.0	30.0	37.0
7	34.20958996139439	37.0	35.0	37.0	30.0	37.0
8	34.14065493633042	37.0	35.0	37.0	30.0	37.0
9	35.67895932343534	39.0	37.0	39.0	30.0	39.0
10-11	35.59939782817156	39.0	36.0	39.0	30.0	39.0
12-13	35.5167423339069	39.0	35.0	39.0	30.0	39.0
14-15	36.66538264432123	40.0	37.0	41.0	30.0	41.0
16-17	36.63677535538402	40.0	37.0	41.0	30.0	41.0
18-19	36.64419418217175	40.0	37.0	41.0	30.0	41.0
20-21	36.69228837735878	40.0	37.0	41.0	30.0	41.0
22-23	36.649189392358885	40.0	37.0	41.0	30.0	41.0
24-25	36.645471186476364	40.0	37.0	41.0	30.0	41.0
26-27	36.640613785091006	40.0	37.0	41.0	30.0	41.0
28-29	36.621165637566676	40.0	37.0	41.0	30.0	41.0
30-31	36.569247367756184	40.0	37.0	41.0	30.0	41.0
32-33	36.524265593927225	40.0	37.0	41.0	30.0	41.0
34-35	36.4658723440703	40.0	36.5	41.0	30.0	41.0
36-37	36.352238340322664	40.0	36.0	41.0	30.0	41.0
38-39	36.239737311541674	40.0	36.0	41.0	29.0	41.0
40-41	36.11936730447898	40.0	36.0	41.0	29.0	41.0
42-43	36.0213724464699	40.0	36.0	41.0	28.5	41.0
44-45	35.862042144611195	40.0	35.0	41.0	28.0	41.0
46-47	35.736772149415366	39.5	35.0	41.0	27.0	41.0
48-49	35.63855406985758	39.0	35.0	41.0	27.0	41.0
50-51	35.531810506198525	39.0	35.0	41.0	27.0	41.0
52-53	35.418276988035515	39.0	35.0	41.0	27.0	41.0
54-55	35.27539067359794	39.0	35.0	41.0	27.0	41.0
56-57	35.12199727540515	39.0	35.0	40.5	26.0	41.0
58-59	34.94858021046467	39.0	34.0	40.0	26.0	41.0
60-61	34.75701604706936	38.0	34.0	40.0	26.0	41.0
62-63	34.50077026985643	38.0	34.0	40.0	25.5	41.0
64-65	34.266377236282764	38.0	34.0	40.0	25.0	41.0
66-67	34.052411008052246	37.0	34.0	40.0	25.0	41.0
68-69	33.77316108987622	37.0	33.0	40.0	24.0	41.0
70-71	33.46249710505816	36.0	33.0	39.0	23.5	41.0
72-73	33.13396750104792	36.0	33.0	39.0	23.0	41.0
74-75	32.84288062025445	35.5	33.0	39.0	22.0	40.0
76-77	32.52382106484096	35.0	32.5	38.0	22.0	40.0
78-79	32.17450159150026	35.0	32.0	37.0	21.0	39.0
80-81	31.843772549443692	35.0	32.0	37.0	20.0	39.0
82-83	31.543838032549672	35.0	32.0	36.0	20.0	39.0
84-85	31.249134268838176	35.0	32.0	36.0	18.5	37.5
86-87	30.965162724441733	35.0	32.0	36.0	18.0	37.0
88-89	30.72847108311976	35.0	32.0	35.0	16.0	37.0
90-91	30.508536848542384	35.0	31.0	35.0	9.0	36.0
92-93	30.2877037464854	35.0	31.0	35.0	4.5	36.0
94-95	30.06991631464812	34.5	31.0	35.0	2.0	36.0
96-97	29.861714011327116	34.0	31.0	35.0	2.0	36.0
98-99	29.67107981809238	34.0	31.0	35.0	2.0	35.0
100	29.52061031116079	34.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.36937483531494664
1101	2	0.34879815493883015
1101	3	0.38360422895508606
1101	4	0.4908050069851413
1101	5	0.4645606837745504
1101	6	0.5407847167730111
1101	7	0.5996422052557193
1101	8	0.5750282486515772
1101	9	0.7676643228313011
1101	10-11	0.7269408611983366
1101	12-13	0.7619420311986502
1101	14-15	0.8423639659039637
1101	16-17	0.768061459857428
1101	18-19	0.8230954621708619
1101	20-21	0.9009899033228237
1101	22-23	0.7967865316795297
1101	24-25	0.7857324020179561
1101	26-27	0.7619897179877455
1101	28-29	0.7786098031982647
1101	30-31	0.8137683793342632
1101	32-33	0.8507167132474365
1101	34-35	0.8259021725286999
1101	36-37	0.7583267583905737
1101	38-39	0.7892934552300375
1101	40-41	0.8152925921157745
1101	42-43	0.7452321891995624
1101	44-45	0.7988795210989608
1101	46-47	0.7225428844335511
1101	48-49	0.694041335462309
1101	50-51	0.6937914443299746
1101	52-53	0.7285262528702816
1101	54-55	0.6800002395308624
1101	56-57	0.5991376274340467
1101	58-59	0.5919947823538578
1101	60-61	0.714058651996524
1101	62-63	0.6706681836920794
1101	64-65	0.6814274368388169
1101	66-67	0.6395696557892734
1101	68-69	0.5878687891358254
1101	70-71	0.6632647476786815
1101	72-73	0.6315833952905976
1101	74-75	0.6065057426332174
1101	76-77	0.636985840691807
1101	78-79	0.542961655289055
1101	80-81	0.6460355854386357
1101	82-83	0.5969383714406575
1101	84-85	0.5770688189119539
1101	86-87	0.6346668203077002
1101	88-89	0.533830105579959
1101	90-91	0.5902667456469466
1101	92-93	0.5650422403826276
1101	94-95	0.562736085048229
1101	96-97	0.5593624290616894
1101	98-99	0.6509346853234135
1101	100	0.681329194890786
1102	1	0.12020120363262521
1102	2	0.11731646639939086
1102	3	0.1665973274096011
1102	4	0.18430152628562269
1102	5	0.2630085055275728
1102	6	0.3590923785083291
1102	7	0.40968800592427357
1102	8	0.41515821810472886
1102	9	0.4611618084022595
1102	10-11	0.5071816348198794
1102	12-13	0.478162982140212
1102	14-15	0.6157079011569948
1102	16-17	0.5340670263484029
1102	18-19	0.5310310164575682
1102	20-21	0.5296310520560965
1102	22-23	0.5279383216735098
1102	24-25	0.5050140008985409
1102	26-27	0.44623563305168545
1102	28-29	0.4673780297512593
1102	30-31	0.4074708716108617
1102	32-33	0.5068996765074587
1102	34-35	0.4756363846491354
1102	36-37	0.5004739196176615
1102	38-39	0.553330549304647
1102	40-41	0.5277538536420607
1102	42-43	0.5021086392609604
1102	44-45	0.47087343017157934
1102	46-47	0.49615512539092776
1102	48-49	0.43171230390992577
1102	50-51	0.4445275491810392
1102	52-53	0.364878460889436
1102	54-55	0.3475589533000658
1102	56-57	0.3676840987771399
1102	58-59	0.4876562334237633
1102	60-61	0.439643605917297
1102	62-63	0.37562409111365014
1102	64-65	0.43523474958484343
1102	66-67	0.45796371590929397
1102	68-69	0.39899018688637966
1102	70-71	0.3459815535131412
1102	72-73	0.3990571545592658
1102	74-75	0.41721069161109625
1102	76-77	0.31990350655116373
1102	78-79	0.4059045115437314
1102	80-81	0.4445425153372575
1102	82-83	0.3992607583650667
1102	84-85	0.37118629951242
1102	86-87	0.3745078528013863
1102	88-89	0.35398693293519656
1102	90-91	0.3621756450035818
1102	92-93	0.27058581970579
1102	94-95	0.26184224620414653
1102	96-97	0.29979376999498797
1102	98-99	0.3663464675618293
1102	100	0.410150381900511
1103	1	0.18879711104868235
1103	2	0.2699082058247946
1103	3	0.1682775583110967
1103	4	0.3149950415035079
1103	5	0.34113528083385347
1103	6	0.35886135109446826
1103	7	0.42663610764324744
1103	8	0.47125762327849685
1103	9	0.5668474330735762
1103	10-11	0.5816242565072329
1103	12-13	0.5891726015508283
1103	14-15	0.6116834089431151
1103	16-17	0.6006542224303146
1103	18-19	0.6111009430668801
1103	20-21	0.5387708273496514
1103	22-23	0.561178698041175
1103	24-25	0.5554087229732261
1103	26-27	0.5474578934553804
1103	28-29	0.46731314999716034
1103	30-31	0.5509710153627339
1103	32-33	0.5575786076529994
1103	34-35	0.5614098872400177
1103	36-37	0.5770491564294318
1103	38-39	0.5691034791289411
1103	40-41	0.6641159439215514
1103	42-43	0.6310054228017989
1103	44-45	0.6149595325414268
1103	46-47	0.540314414897864
1103	48-49	0.5176811260401379
1103	50-51	0.5221478382326126
1103	52-53	0.4515275200302966
1103	54-55	0.4505374622283256
1103	56-57	0.4525485911948408
1103	58-59	0.41446142654654494
1103	60-61	0.41140840112230137
1103	62-63	0.5131346224022693
1103	64-65	0.4675787609287667
1103	66-67	0.4587833963717003
1103	68-69	0.42819549339933616
1103	70-71	0.4370540940135754
1103	72-73	0.5476325117400549
1103	74-75	0.5161110731504692
1103	76-77	0.4257487962423312
1103	78-79	0.40022002333520845
1103	80-81	0.44040556399368214
1103	82-83	0.38594844390228644
1103	84-85	0.3526598083645176
1103	86-87	0.31078175516839934
1103	88-89	0.34026317092000724
1103	90-91	0.3634670242209701
1103	92-93	0.3311687973765096
1103	94-95	0.42860577009142986
1103	96-97	0.34244848679979967
1103	98-99	0.3306840515416951
1103	100	0.2815578022396039
1104	1	0.07170103001846329
1104	2	0.09561738960942279
1104	3	0.16980650237349693
1104	4	0.21375775169427413
1104	5	0.3054052602869035
1104	6	0.31684607271367327
1104	7	0.31574722563261304
1104	8	0.3342765393434135
1104	9	0.5000377157573368
1104	10-11	0.44822694003563157
1104	12-13	0.5043892982211133
1104	14-15	0.6095990030691212
1104	16-17	0.5537770627081784
1104	18-19	0.48976546709717894
1104	20-21	0.48378013360392913
1104	22-23	0.49410799956634577
1104	24-25	0.5378818438212036
1104	26-27	0.5441393441900928
1104	28-29	0.5352613252936536
1104	30-31	0.45493531250532726
1104	32-33	0.43533762127631803
1104	34-35	0.47284187068326133
1104	36-37	0.48401075587067055
1104	38-39	0.4907404556686217
1104	40-41	0.4891629846617249
1104	42-43	0.5441394416958261
1104	44-45	0.4605546152816018
1104	46-47	0.4328647673773247
1104	48-49	0.4520049128240089
1104	50-51	0.40390957421148244
1104	52-53	0.3804850641509958
1104	54-55	0.4187059533437747
1104	56-57	0.35534118687724003
1104	58-59	0.46700358502662453
1104	60-61	0.48156404845221346
1104	62-63	0.46067090675816047
1104	64-65	0.4551876986092367
1104	66-67	0.48814917861843554
1104	68-69	0.3820979873669259
1104	70-71	0.38713153967314895
1104	72-73	0.38560202405115973
1104	74-75	0.40789244774391165
1104	76-77	0.3933212216500195
1104	78-79	0.3429486758176026
1104	80-81	0.39246114391754006
1104	82-83	0.3902074623514835
1104	84-85	0.28622457622556396
1104	86-87	0.2837251211049221
1104	88-89	0.324731297386343
1104	90-91	0.2793074617640485
1104	92-93	0.2514648468862255
1104	94-95	0.29664777981430035
1104	96-97	0.3503894414639568
1104	98-99	0.34369644819793166
1104	100	0.3122053465704937
1105	1	0.1018316985313632
1105	2	0.09496860593708334
1105	3	0.1468000924287729
1105	4	0.11047568148623554
1105	5	0.18592095477574588
1105	6	0.21279102616989576
1105	7	0.31875467097404453
1105	8	0.24189113292285924
1105	9	0.28939292358175805
1105	10-11	0.387116133183639
1105	12-13	0.3847022212781184
1105	14-15	0.48278153659097
1105	16-17	0.4252102477919806
1105	18-19	0.4417234469727731
1105	20-21	0.38123047046123304
1105	22-23	0.41803312555022387
1105	24-25	0.34969389719243793
1105	26-27	0.4309252572907383
1105	28-29	0.44136846268072816
1105	30-31	0.4389553029040556
1105	32-33	0.377699139401642
1105	34-35	0.3982566468331612
1105	36-37	0.40690063498772844
1105	38-39	0.395040406248782
1105	40-41	0.36874484921640516
1105	42-43	0.369112858147588
1105	44-45	0.338529592095675
1105	46-47	0.3317923551130235
1105	48-49	0.39661992967742776
1105	50-51	0.36179171644776886
1105	52-53	0.31143745206024676
1105	54-55	0.29410628613415923
1105	56-57	0.23926076677089725
1105	58-59	0.23332904527431708
1105	60-61	0.2509374608782764
1105	62-63	0.3417360907247584
1105	64-65	0.3187276819914473
1105	66-67	0.2663855344136081
1105	68-69	0.25094285549656803
1105	70-71	0.28561598710807345
1105	72-73	0.2529509362340647
1105	74-75	0.2478556289770708
1105	76-77	0.13961615093074897
1105	78-79	0.13720617238569588
1105	80-81	0.24810927416116257
1105	82-83	0.08382976826286281
1105	84-85	0.031305161980398566
1105	86-87	0.0726260879571683
1105	88-89	0.09665224522235505
1105	90-91	0.18251620011267988
1105	92-93	0.1437317570734038
1105	94-95	0.18159691264208533
1105	96-97	0.15093345178016548
1105	98-99	0.16864280598273496
1105	100	0.12779025468203287
1106	1	0.07678429478217907
1106	2	0.09497654329768324
1106	3	0.12122081225164294
1106	4	0.10375911137165872
1106	5	0.16929992630434043
1106	6	0.13513728749354925
1106	7	0.2074546561079771
1106	8	0.11708195012444378
1106	9	0.21236628523787004
1106	10-11	0.21134955938246236
1106	12-13	0.1586614067837928
1106	14-15	0.26732139473104155
1106	16-17	0.20968434795234003
1106	18-19	0.19707854604678943
1106	20-21	0.20025812376152174
1106	22-23	0.21825636564872042
1106	24-25	0.2666802054407853
1106	26-27	0.24777036844199785
1106	28-29	0.19294161702275403
1106	30-31	0.2515111254452904
1106	32-33	0.2376178764596233
1106	34-35	0.20839283215866544
1106	36-37	0.22860958975432055
1106	38-39	0.23384813311545827
1106	40-41	0.25508554549779205
1106	42-43	0.1933733080064215
1106	44-45	0.17293253564998423
1106	46-47	0.20647927202993088
1106	48-49	0.11543336216312383
1106	50-51	0.12194208034156162
1106	52-53	0.1456147059244728
1106	54-55	0.05003330256749905
1106	56-57	0.027307216498186904
1106	58-59	0.12292098214685865
1106	60-61	0.11147869459956894
1106	62-63	0.15416037288391493
1106	64-65	0.18437227223692787
1106	66-67	0.2574739788108502
1106	68-69	0.247902638795388
1106	70-71	0.24004070052234283
1106	72-73	0.2492228678635442
1106	74-75	0.24192115753043453
1106	76-77	0.171018877196758
1106	78-79	0.16704239337559557
1106	80-81	0.173661984939546
1106	82-83	0.21866380086138548
1106	84-85	0.174957992964611
1106	86-87	0.15800488115158373
1106	88-89	0.18871196367660303
1106	90-91	0.19034008087584198
1106	92-93	0.06674719895200809
1106	94-95	0.15277049487867345
1106	96-97	0.22050208505044466
1106	98-99	0.28251286494218064
1106	100	0.2619351412108699
1107	1	0.10634185614053493
1107	2	0.11544797155021413
1107	3	0.08073374381455167
1107	4	0.10717397651833949
1107	5	0.19181386572923742
1107	6	0.24658077673253587
1107	7	0.22269687663219173
1107	8	0.25988812774665604
1107	9	0.24507263044714023
1107	10-11	0.2928711265905548
1107	12-13	0.3226314805762911
1107	14-15	0.41928655849399377
1107	16-17	0.35562991480994555
1107	18-19	0.3147937707792394
1107	20-21	0.2961612596650838
1107	22-23	0.3507465511404533
1107	24-25	0.3971118957827997
1107	26-27	0.4121612064118594
1107	28-29	0.385077941846788
1107	30-31	0.380207903665152
1107	32-33	0.3762222854350483
1107	34-35	0.31959055730086305
1107	36-37	0.27482080816671584
1107	38-39	0.28948143465193965
1107	40-41	0.39270443901262553
1107	42-43	0.39372719971097325
1107	44-45	0.28249039305213586
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2216	88-89	-1.2549624645178667
2216	90-91	-1.3626034744096849
2216	92-93	-1.2762387206941845
2216	94-95	-1.208443603454505
2216	96-97	-1.2900093935995294
2216	98-99	-1.3149347633998083
2216	100	-1.3729586835143301
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	95025.0
3	16231.0
4	10389.0
5	8423.0
6	7122.0
7	6941.0
8	7489.0
9	8473.0
10	9555.0
11	10480.0
12	11740.0
13	12766.0
14	14132.0
15	14814.0
16	14935.0
17	15552.0
18	14807.0
19	14065.0
20	14261.0
21	15213.0
22	17146.0
23	20680.0
24	25220.0
25	31502.0
26	38040.0
27	42897.0
28	51058.0
29	63855.0
30	75463.0
31	86492.0
32	110388.0
33	143851.0
34	197387.0
35	283643.0
36	462854.0
37	807318.0
38	1035565.0
39	363244.0
40	688.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.08084992576414	21.072856013080642	14.319439978374513	22.526854082780705
2	15.569715662604475	21.13244432533577	40.48650575829287	22.81133425376688
3	17.763878755625406	26.52801570507613	30.894937127769573	24.813168411528892
4	12.412072133033506	18.201373538682997	39.68400762045501	29.702546707828482
5	12.421504073473503	37.94650708230955	35.49543625969258	14.136552584524365
6	28.667282541154606	39.01227513941517	18.857339876820316	13.4631024426099
7	25.457995237524344	33.21782499253993	23.645828023945906	17.67835174598981
8	23.791562453715812	36.202340894250604	21.80285274503672	18.203243906996867
9	24.14269337806768	17.910463831720758	22.84712965375625	35.099713136455314
10-11	22.638954990420363	27.77708386124828	29.808321112027556	19.7756400363038
12-13	23.348169520900935	25.65142572528163	29.51211671171442	21.48828804210301
14-15	21.09999534611004	28.049604963702652	26.811287393975952	24.039112296211364
16-17	20.304916515910485	31.097615470102657	27.349992863482914	21.24747515050394
18-19	20.19197582033988	29.902745155651655	29.43518782269286	20.4700912013156
20-21	21.615184621942774	29.377026640781178	28.707540518458725	20.300248218817323
22-23	21.184643327203332	29.429694651346733	28.741939046081722	20.643722975368213
24-25	20.916104311562613	29.350790778776126	28.857176938493467	20.875927971167794
26-27	20.714478748566364	29.22658469822128	28.947567702266387	21.11136885094597
28-29	20.73467699135584	29.28883207895793	29.031835468571067	20.94465546111516
30-31	21.115881461148405	29.8550375087814	28.436207315695878	20.592873714374317
32-33	20.600629975220208	29.448362514273025	29.040402831921426	20.91060467858534
34-35	20.781639899056483	28.95590408786785	29.103354646768274	21.159101366307386
36-37	21.319325754568517	29.44493934284352	28.468725332345613	20.767009570242354
38-39	21.076481761213227	29.471223247536006	28.674130685132287	20.778164306118484
40-41	20.81783546023434	29.219199117534984	28.878117296302346	21.084848125928332
42-43	20.727483632192524	29.3132998021483	28.822032290334988	21.13718427532419
44-45	21.0457485997451	29.427966618822932	28.613789525523813	20.91249525590816
46-47	21.01347255076809	29.64201658371777	28.3860278295282	20.958483035985946
48-49	20.8363568883273	29.279251092398205	28.70483048131087	21.179561537963625
50-51	20.934552313918804	29.096726963670456	28.679095668169996	21.289625054240744
52-53	20.916313736750173	29.208073122293204	28.60457492881583	21.271038212140787
54-55	21.071198351082234	29.68291257759212	28.387888092196977	20.858000979128672
56-57	20.843935713650723	29.370026273682537	28.394744880616212	21.39129313205053
58-59	20.693592176796084	29.0275366107066	28.80505213694794	21.47381907554938
60-61	21.14019369585248	29.418314276150458	28.32861061017998	21.112881417817082
62-63	20.935997397527963	29.432436885087913	28.44081272454726	21.190752992836863
64-65	20.96332808255115	29.060452995129516	28.685376043151738	21.290842879167595
66-67	20.811217846103485	29.193711329302896	28.45600998086236	21.53906084373126
68-69	21.274328909848048	29.363997269290067	28.355414867150674	21.006258953711214
70-71	21.298219197554825	29.068236413525998	28.471839658379995	21.16170473053918
72-73	20.923459873224797	29.214543181651216	28.800330613873097	21.061666331250883
74-75	20.931671957723868	29.254913935021037	28.59295846892004	21.220455638335054
76-77	21.09151223368581	29.32556508581179	28.287154779448624	21.295767901053782
78-79	21.169372929207274	29.372960313280565	28.459640074778843	20.998026682733318
80-81	20.979488275339577	29.204625972169413	28.593236972074408	21.222648780416602
82-83	20.838378631044083	29.27175551714596	28.73165492664124	21.158210925168717
84-85	20.944966446807143	29.199718275450472	28.533140859044526	21.32217441869786
86-87	21.153065469449757	29.31143875527919	28.443102345271644	21.092393429999408
88-89	20.870786617676792	29.293112504023	28.51230948327329	21.323791395026916
90-91	20.74301271998189	29.15689381478378	28.597897056156192	21.502196409078138
92-93	20.93621762881032	29.40992725854948	28.382110170822717	21.271744941817484
94-95	20.866205322211297	29.175514286603065	28.575443457786264	21.38283693339938
96-97	20.860023782271263	29.26340402810275	28.545856126471243	21.330716063154746
98-99	20.806563493064267	29.261498160337418	28.547024273688002	21.384914072910313
100	20.992401571798172	29.21933848668367	28.398913054141282	21.389346887376874
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3744.0
1	2786.5
2	2076.5
3	2285.5
4	2010.0
5	1628.5
6	1418.5
7	1376.5
8	1358.0
9	1326.5
10	1199.5
11	1069.5
12	1068.5
13	1108.5
14	1187.0
15	1327.5
16	1582.0
17	1895.0
18	2453.0
19	3392.0
20	4791.0
21	6786.0
22	10037.5
23	15267.5
24	21700.5
25	29484.5
26	39854.5
27	51981.0
28	65983.5
29	80336.0
30	92411.5
31	104108.5
32	115605.5
33	125689.0
34	134207.5
35	140724.5
36	145930.5
37	149616.5
38	152277.5
39	152882.5
40	152158.0
41	150421.0
42	146475.0
43	142321.0
44	137832.0
45	133834.5
46	130513.5
47	127004.0
48	124035.5
49	121565.5
50	118137.0
51	111764.5
52	103090.0
53	96029.0
54	91350.5
55	86904.0
56	79902.5
57	71998.0
58	65706.0
59	59539.0
60	51856.0
61	43100.5
62	35539.5
63	28886.5
64	22988.0
65	18087.5
66	13980.5
67	10679.0
68	8013.5
69	5884.5
70	4262.5
71	3117.0
72	2372.5
73	1773.5
74	1314.0
75	940.5
76	614.5
77	421.5
78	294.5
79	202.5
80	133.0
81	93.0
82	85.0
83	65.5
84	49.0
85	46.0
86	41.0
87	28.5
88	19.0
89	15.0
90	12.0
91	11.0
92	12.0
93	13.5
94	11.0
95	10.0
96	10.0
97	6.0
98	5.0
99	7.5
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07538811360804497
2	0.01229752154698036
3	0.016101618679217475
4	0.010192109297691895
5	0.008302023301171566
6	0.006722964114205216
7	0.018781234269220976
8	0.008636975249922005
9	0.008732675806707843
10-11	0.012608548356534337
12-13	0.014845548871403333
14-15	0.009677718804968007
16-17	0.011830981332649393
18-19	0.007739782530054759
20-21	0.012644436065329027
22-23	0.012692286343721947
24-25	0.015013024845778551
26-27	0.005012316661658338
28-29	0.011149114865550288
30-31	0.009952857905727296
32-33	0.011771168484658244
34-35	0.008062771909206968
36-37	0.009450429982601639
38-39	0.018338619194086473
40-41	0.011998457307024611
42-43	0.007440718290099012
44-45	0.019666464419489993
46-47	0.017357688487031616
48-49	0.016998811399084723
50-51	0.010036595892914906
52-53	0.009665756235369777
54-55	0.011436216535907807
56-57	0.019319549901141327
58-59	0.0020695245404937766
60-61	0.006340161887061859
62-63	0.014367046087474137
64-65	0.023004021337396142
66-67	0.012106120433408682
68-69	0.01366125448117857
70-71	0.0245471928155678
72-73	0.019403287888328936
74-75	0.019080298509176724
76-77	0.00916332831224412
78-79	0.01069453722081755
80-81	0.0185898331556493
82-83	0.011543879662291876
84-85	0.010634724372826402
86-87	0.016233206944798007
88-89	0.014570409770644046
90-91	0.014594334909840504
92-93	0.007476605998893702
94-95	0.014690035466626344
96-97	0.012931537735686548
98-99	0.009689681374566236
100	0.006866514949383976
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4179704.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.83367580061254
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.95298645610717	56.532446112671664
2	12.660775835238805	17.682970301245927
3	3.217213383538422	6.740095092222417
4	1.1833960985078396	3.3056359794762487
5	0.589311343956557	2.057688866974274
6	0.3292883243854476	1.379724845403617
7	0.2144702517956592	1.0484072222941665
8	0.14701495503947837	0.8213275766454848
9	0.11396548600048062	0.716276592163513
>10	0.5501411288861838	6.717964310767351
>50	0.029626400231316616	1.4131337753433524
>100	0.011467302286576673	1.3435544444443333
>500	3.08730655967855E-4	0.13573087121185737
>1k	3.430337007935224E-5	0.10504400913580786
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4385	0.10491173537647643	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	2.3925139196459845E-5	0.0
12-13	0.0	0.0	0.0	2.3925139196459845E-5	0.0
14-15	0.0	2.3925139196459845E-5	0.0	3.588770879468977E-5	0.0
16-17	0.0	2.3925139196459845E-5	0.0	7.177541758937954E-5	0.0
18-19	0.0	2.3925139196459845E-5	0.0	8.373798718760945E-5	0.0
20-21	0.0	2.3925139196459845E-5	0.0	1.674759743752189E-4	0.0
22-23	0.0	2.3925139196459845E-5	0.0	2.2728882236636852E-4	0.0
24-25	1.1962569598229923E-5	2.3925139196459845E-5	0.0	3.828022271433575E-4	0.0
26-27	2.3925139196459845E-5	2.3925139196459845E-5	0.0	5.742033407150363E-4	0.0
28-29	2.3925139196459845E-5	2.3925139196459845E-5	0.0	0.0012680323774123719	0.0
30-31	2.3925139196459845E-5	2.3925139196459845E-5	0.0	0.0029786798299592508	0.0
32-33	2.3925139196459845E-5	2.3925139196459845E-5	0.0	0.0071416540501432636	0.0
34-35	2.3925139196459845E-5	2.3925139196459845E-5	0.0	0.014678072897028115	0.0
36-37	2.3925139196459845E-5	2.3925139196459845E-5	0.0	0.025312797269854515	0.0
38-39	3.588770879468977E-5	2.3925139196459845E-5	0.0	0.03842377354951451	0.0
40-41	4.785027839291969E-5	2.3925139196459845E-5	0.0	0.0534487609648913	0.0
42-43	5.981284799114961E-5	2.3925139196459845E-5	0.0	0.07051934778156539	0.0
44-45	7.177541758937954E-5	2.3925139196459845E-5	0.0	0.08895366753243769	0.0
46-47	7.177541758937954E-5	2.3925139196459845E-5	0.0	0.1107853570492073	0.0
48-49	7.177541758937954E-5	2.3925139196459845E-5	0.0	0.13241368288280703	0.0
50-51	7.177541758937954E-5	2.3925139196459845E-5	0.0	0.1555492924857837	0.0
52-53	7.177541758937954E-5	2.3925139196459845E-5	0.0	0.1783379875704117	0.0
54-55	7.177541758937954E-5	2.3925139196459845E-5	0.0	0.20631843786067147	0.0
56-57	7.177541758937954E-5	4.785027839291969E-5	0.0	0.2375048568032569	0.0
58-59	7.177541758937954E-5	4.785027839291969E-5	0.0	0.27163406786700683	0.0
60-61	7.177541758937954E-5	4.785027839291969E-5	0.0	0.30654084595464176	0.0
62-63	7.177541758937954E-5	4.785027839291969E-5	0.0	0.3417108005734377	0.0
64-65	7.177541758937954E-5	4.785027839291969E-5	0.0	0.38216821095465137	0.0
66-67	7.177541758937954E-5	7.177541758937954E-5	1.1962569598229923E-5	0.43114297088980463	0.0
68-69	7.177541758937954E-5	7.177541758937954E-5	2.3925139196459845E-5	0.4846635072722853	0.0
70-71	7.177541758937954E-5	7.177541758937954E-5	2.3925139196459845E-5	0.5423829055837447	0.0
72-73	7.177541758937954E-5	7.177541758937954E-5	2.3925139196459845E-5	0.607590872463696	0.0
74-75	7.177541758937954E-5	7.177541758937954E-5	2.3925139196459845E-5	0.6749042515929358	0.0
76-77	7.177541758937954E-5	7.177541758937954E-5	2.3925139196459845E-5	0.7458422893104393	0.0
78-79	7.177541758937954E-5	7.177541758937954E-5	2.3925139196459845E-5	0.8283600943990292	0.0
80-81	7.177541758937954E-5	7.177541758937954E-5	2.3925139196459845E-5	0.9198857143950864	0.0
82-83	7.177541758937954E-5	7.177541758937954E-5	2.3925139196459845E-5	1.0182180364925364	0.0
84-85	7.177541758937954E-5	9.570055678583938E-5	2.3925139196459845E-5	1.1183447440297207	0.0
86-87	7.177541758937954E-5	9.570055678583938E-5	2.3925139196459845E-5	1.2310680373538414	0.0
88	7.177541758937954E-5	9.570055678583938E-5	2.3925139196459845E-5	1.3241607539672666	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4660	0.0	43.69558	1
GTATCAA	7670	0.0	34.702164	1
TCAACGC	8095	0.0	32.10698	4
ATCAACG	8130	0.0	31.97067	3
CAACGCA	8935	0.0	29.141829	5
TATCAAC	9185	0.0	28.656689	2
AACGCAG	9550	0.0	27.414112	6
GTACCTG	3090	0.0	25.871758	1
ACGCAGA	10665	0.0	24.41611	7
CGCAGAG	10565	0.0	24.243616	8
TACCTGG	3230	0.0	24.010355	2
GTACATG	11955	0.0	23.876717	1
TACATGG	11870	0.0	23.283258	2
ACATGGG	12195	0.0	21.814827	3
TCTGTCG	1185	0.0	21.019766	8
GCAGAGT	12055	0.0	20.156712	9
GTCGTAC	260	1.6754711E-8	19.895498	1
GTGGTAT	2725	0.0	19.328005	1
CATGGGG	7125	0.0	18.997602	4
TAGACCG	185	1.6508874E-4	17.783936	5
>>END_MODULE