FastQCFastQC Report
Wed 25 May 2016
SRR2049444_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049444_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4179704
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154200.3689256464094108No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147630.35320682995733665No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89030.213005514266082No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42310.1012272639402216No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42280.10115548852263222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86500.058.490791
GTATCAA155150.039.132091
TATAACG5050.035.370942
GTGGTAT33700.034.3558241
TGGTATC35200.032.1831672
ATCAACG192550.031.05263
TCAACGC193650.030.8519364
TATCAAC197650.030.489172
CAACGCA201500.029.6966695
ATAACGC5950.029.2306983
AACGCAG210900.028.413226
ACGCAGA236350.025.3139347
CGCAGAG236500.025.2382628
GTACCTG31650.024.8334961
TAACGCA7000.024.1745764
TACCTGG31500.023.5776732
GCAGAGT257350.022.9195679
GTACATG118100.022.3168681
TACATGG117900.021.8484762
CAGAGTA256700.020.85377710-11
Produced by FastQC (version 0.11.5)