##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049444_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4179704 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.8683215366447 31.0 31.0 34.0 30.0 34.0 2 31.93888012165455 33.0 31.0 34.0 30.0 34.0 3 32.0225214512798 33.0 31.0 34.0 30.0 34.0 4 35.77323274566812 37.0 35.0 37.0 33.0 37.0 5 35.868163630726 37.0 35.0 37.0 35.0 37.0 6 35.96617033167899 37.0 35.0 37.0 35.0 37.0 7 35.929012915747144 37.0 35.0 37.0 35.0 37.0 8 35.939760566776975 37.0 35.0 37.0 35.0 37.0 9 37.64952972746396 39.0 37.0 39.0 35.0 39.0 10-11 37.63280940468512 39.0 37.0 39.0 35.0 39.0 12-13 37.6334320564327 39.0 37.0 39.0 35.0 39.0 14-15 38.93357986594266 40.0 38.0 41.0 35.5 41.0 16-17 38.94418241100327 40.0 38.0 41.0 35.5 41.0 18-19 39.02717800112161 40.0 38.5 41.0 36.0 41.0 20-21 39.05296439652186 40.0 39.0 41.0 36.0 41.0 22-23 39.01866089081906 40.0 39.0 41.0 35.0 41.0 24-25 39.00178840415494 40.0 39.0 41.0 35.0 41.0 26-27 38.90648895232772 40.0 38.0 41.0 35.0 41.0 28-29 38.81854923219443 40.0 38.0 41.0 35.0 41.0 30-31 38.6936097627966 40.0 38.0 41.0 35.0 41.0 32-33 38.53756581805793 40.0 38.0 41.0 34.5 41.0 34-35 38.409051932864145 40.0 38.0 41.0 34.0 41.0 36-37 38.26205539913831 40.0 38.0 41.0 34.0 41.0 38-39 38.145153939130616 40.0 38.0 41.0 34.0 41.0 40-41 38.0493335173974 40.0 38.0 41.0 34.0 41.0 42-43 37.90006600945904 40.0 38.0 41.0 33.0 41.0 44-45 37.715072048164174 40.0 38.0 41.0 33.0 41.0 46-47 37.443561673266814 40.0 37.0 41.0 33.0 41.0 48-49 37.175416967325916 40.0 37.0 41.0 32.0 41.0 50-51 37.01990308404614 40.0 37.0 41.0 31.5 41.0 52-53 36.90954682436843 40.0 37.0 41.0 31.0 41.0 54-55 36.77234919027759 40.0 36.5 41.0 31.0 41.0 56-57 36.63943008882926 40.0 36.0 41.0 31.0 41.0 58-59 36.47630023561477 39.5 36.0 41.0 31.0 41.0 60-61 36.295540904331986 39.0 35.0 41.0 31.0 41.0 62-63 36.078678777253124 39.0 35.0 41.0 30.0 41.0 64-65 35.864980390955914 39.0 35.0 41.0 30.0 41.0 66-67 35.612712168134394 38.0 35.0 40.0 30.0 41.0 68-69 35.32604952408113 37.5 35.0 40.0 29.0 41.0 70-71 35.04033993794776 37.0 35.0 40.0 29.0 41.0 72-73 34.709351667008 36.5 34.5 39.0 29.0 41.0 74-75 34.38849018973592 36.0 34.0 39.0 29.0 41.0 76-77 34.07116652279683 36.0 34.0 39.0 29.0 40.5 78-79 33.756360498255376 35.0 34.0 37.5 29.0 39.5 80-81 33.4407186489761 35.0 34.0 37.0 28.0 39.0 82-83 33.157711048437875 35.0 34.0 37.0 28.0 39.0 84-85 32.86408702625832 35.0 34.0 36.0 28.0 38.0 86-87 32.604541493847414 35.0 34.0 36.0 27.0 37.0 88-89 32.37357537758655 35.0 34.0 36.0 27.0 37.0 90-91 32.17561506747846 35.0 33.5 35.0 27.0 36.5 92-93 32.00214010370112 35.0 33.0 35.0 26.0 36.0 94-95 31.834741527151206 35.0 33.0 35.0 26.0 36.0 96-97 31.687077482041794 35.0 33.0 35.0 26.0 36.0 98-99 31.552537332787203 35.0 33.0 35.0 25.5 36.0 100 31.443542892032546 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28060968771480077 1101 2 0.31397278769367887 1101 3 0.3898904930604772 1101 4 0.21650557022191208 1101 5 0.19051912707886487 1101 6 0.21498745865487479 1101 7 0.24106333437104865 1101 8 0.23098823920452105 1101 9 0.31652020089650534 1101 10-11 0.25608941900753734 1101 12-13 0.2888580015241615 1101 14-15 0.406027014352631 1101 16-17 0.3590774859941206 1101 18-19 0.36385927157179054 1101 20-21 0.39647705351885776 1101 22-23 0.40873160582493284 1101 24-25 0.3904744300187559 1101 26-27 0.36614190473977004 1101 28-29 0.42590075137966465 1101 30-31 0.3975845179130175 1101 32-33 0.3557321034442751 1101 34-35 0.36883046148862064 1101 36-37 0.4362356031592398 1101 38-39 0.42825682414176924 1101 40-41 0.38378994298143 1101 42-43 0.3467994283459248 1101 44-45 0.36761439293454146 1101 46-47 0.3458139570864134 1101 48-49 0.39304775506619904 1101 50-51 0.313774508971548 1101 52-53 0.2860832258040418 1101 54-55 0.32878774078039896 1101 56-57 0.33809508599610893 1101 58-59 0.2626299498784874 1101 60-61 0.17925753126635158 1101 62-63 0.23409546713259033 1101 64-65 0.15326276904165326 1101 66-67 0.23440167926004563 1101 68-69 0.19798542732513624 1101 70-71 0.20396374873121914 1101 72-73 0.2727980470796041 1101 74-75 0.22085919242844199 1101 76-77 0.2039309662933988 1101 78-79 0.22222330148726144 1101 80-81 0.19835104080257793 1101 82-83 0.18354859194555218 1101 84-85 0.1597243259515153 1101 86-87 0.15247268152566562 1101 88-89 0.21147442115224635 1101 90-91 0.22348910007134748 1101 92-93 0.21979437954538028 1101 94-95 0.1589589399391116 1101 96-97 0.09797271284238462 1101 98-99 0.18173021730890682 1101 100 0.13270550754195654 1102 1 0.18323300715647406 1102 2 0.2280124272008024 1102 3 0.3043579695280627 1102 4 0.22007671265896533 1102 5 0.1578977528777017 1102 6 0.143155214915744 1102 7 0.21601028750212237 1102 8 0.1926710318211775 1102 9 0.26273267184834737 1102 10-11 0.25698563487700454 1102 12-13 0.24348543219826269 1102 14-15 0.3131813555215359 1102 16-17 0.29658605474268995 1102 18-19 0.2978036550307621 1102 20-21 0.2915469676052638 1102 22-23 0.28113833601478433 1102 24-25 0.2102012670107456 1102 26-27 0.2343886886741231 1102 28-29 0.3038247893787158 1102 30-31 0.24745423152314316 1102 32-33 0.2809400489925622 1102 34-35 0.2986042835207101 1102 36-37 0.3000969547198551 1102 38-39 0.3029890084745901 1102 40-41 0.36861398871617723 1102 42-43 0.3185151867984075 1102 44-45 0.3604560679705031 1102 46-47 0.36713604038101977 1102 48-49 0.3466644254074609 1102 50-51 0.32712531634022923 1102 52-53 0.2812965979273443 1102 54-55 0.35181950164932374 1102 56-57 0.36933916586394844 1102 58-59 0.34847826440737606 1102 60-61 0.342429873999059 1102 62-63 0.2845209546754077 1102 64-65 0.2636151783763552 1102 66-67 0.2727195492288601 1102 68-69 0.2730295679410162 1102 70-71 0.24756239729980933 1102 72-73 0.30595146361048364 1102 74-75 0.23936908353196173 1102 76-77 0.2668013412190362 1102 78-79 0.22279460200615375 1102 80-81 0.29937470222670726 1102 82-83 0.25979717490989884 1102 84-85 0.18452928977683314 1102 86-87 0.21337037385642077 1102 88-89 0.14414807610788216 1102 90-91 0.13997751744231834 1102 92-93 0.14943453876245982 1102 94-95 0.06294202280685113 1102 96-97 0.09787315937529684 1102 98-99 0.15375349824332574 1102 100 0.22732144291027723 1103 1 0.23292873791659474 1103 2 0.24011511573831257 1103 3 0.33463261817232137 1103 4 0.2538222947598783 1103 5 0.21980261552189972 1103 6 0.21019323458503436 1103 7 0.2344843172863591 1103 8 0.17593872464616567 1103 9 0.24548495478468624 1103 10-11 0.2370019103047909 1103 12-13 0.21377594703697866 1103 14-15 0.29041192320151055 1103 16-17 0.3082055731871236 1103 18-19 0.29078440994032206 1103 20-21 0.26376202616951616 1103 22-23 0.2960009618056674 1103 24-25 0.2858142052822217 1103 26-27 0.32465079247645434 1103 28-29 0.34512399418289164 1103 30-31 0.3780445980694864 1103 32-33 0.30703975748985357 1103 34-35 0.36815517983371393 1103 36-37 0.34378908065199454 1103 38-39 0.37972657805926247 1103 40-41 0.3923192821437951 1103 42-43 0.3558301824597052 1103 44-45 0.3585392207125082 1103 46-47 0.33535823294376144 1103 48-49 0.3793363436171333 1103 50-51 0.3944428364962391 1103 52-53 0.39398213981828434 1103 54-55 0.4481566260286911 1103 56-57 0.3710757263710249 1103 58-59 0.3453345175231419 1103 60-61 0.2971006013771387 1103 62-63 0.34659447266722765 1103 64-65 0.29208848013077926 1103 66-67 0.3334007535667638 1103 68-69 0.3085878482209665 1103 70-71 0.32143497351611217 1103 72-73 0.27918348297495754 1103 74-75 0.3052415085400284 1103 76-77 0.29543529506194943 1103 78-79 0.2960685611162788 1103 80-81 0.25954450897653913 1103 82-83 0.288843163210899 1103 84-85 0.30720019130582443 1103 86-87 0.28856875664318693 1103 88-89 0.2890066930662698 1103 90-91 0.253497479131255 1103 92-93 0.2700002896846563 1103 94-95 0.30883007497870807 1103 96-97 0.3341249540728164 1103 98-99 0.3290265140893247 1103 100 0.27708165326153633 1104 1 0.17007267778124913 1104 2 0.21900073131142506 1104 3 0.28620804294321545 1104 4 0.18420274174630435 1104 5 0.14742545376445548 1104 6 0.11983712331079488 1104 7 0.11355572512609768 1104 8 0.1239728547554364 1104 9 0.17552165113187357 1104 10-11 0.1998705433111283 1104 12-13 0.17349859733674577 1104 14-15 0.29169627103189555 1104 16-17 0.32237836826435284 1104 18-19 0.2628482637400751 1104 20-21 0.2733846503327655 1104 22-23 0.23227577092063711 1104 24-25 0.2562984881566166 1104 26-27 0.27004509330232196 1104 28-29 0.2559830172249846 1104 30-31 0.2993958496512974 1104 32-33 0.2575288104259883 1104 34-35 0.270162911931763 1104 36-37 0.3179275221705211 1104 38-39 0.31579153210683586 1104 40-41 0.33468132189409516 1104 42-43 0.3498466632892985 1104 44-45 0.35476376240883667 1104 46-47 0.3429549761426287 1104 48-49 0.39687944128632324 1104 50-51 0.42939715877714235 1104 52-53 0.4339655848980968 1104 54-55 0.3961532244155279 1104 56-57 0.38710065818732886 1104 58-59 0.4402598983914032 1104 60-61 0.35751634431156987 1104 62-63 0.3695601361846883 1104 64-65 0.3957509694860235 1104 66-67 0.4235592344649177 1104 68-69 0.44511476247257775 1104 70-71 0.410425490765455 1104 72-73 0.3787583427731107 1104 74-75 0.40691522622942955 1104 76-77 0.4138024623726082 1104 78-79 0.41150218653396564 1104 80-81 0.38520630960163516 1104 82-83 0.36054530439565724 1104 84-85 0.3759662944564681 1104 86-87 0.365627117524312 1104 88-89 0.34123173921351224 1104 90-91 0.2980366506392187 1104 92-93 0.33857005533232964 1104 94-95 0.31509172827771437 1104 96-97 0.3311472880846118 1104 98-99 0.37007327690018244 1104 100 0.29776798148167316 1105 1 0.1918418560890771 1105 2 0.19621798303841587 1105 3 0.26502276744316333 1105 4 0.14248819172794214 1105 5 0.13873071865681652 1105 6 0.12430441307850515 1105 7 0.12099125201453376 1105 8 0.12693172685529674 1105 9 0.17854482970836472 1105 10-11 0.15282513622079108 1105 12-13 0.16335197182722538 1105 14-15 0.22433144081085743 1105 16-17 0.2014669084931029 1105 18-19 0.1942056450984282 1105 20-21 0.26224735545574873 1105 22-23 0.2040968851368561 1105 24-25 0.22237549814668967 1105 26-27 0.21715040655814732 1105 28-29 0.297827928333831 1105 30-31 0.276765488997853 1105 32-33 0.2570499831523634 1105 34-35 0.20215835089189937 1105 36-37 0.25391539439358013 1105 38-39 0.31790192082937097 1105 40-41 0.24199019124563392 1105 42-43 0.269896019196878 1105 44-45 0.26548505806421474 1105 46-47 0.2727037341098608 1105 48-49 0.2619673377510523 1105 50-51 0.14935002000703435 1105 52-53 0.12926437938515534 1105 54-55 0.1325466419995891 1105 56-57 0.11640033468454902 1105 58-59 0.20487057337140868 1105 60-61 0.17003043710713683 1105 62-63 0.14173639946294259 1105 64-65 0.12951772347713586 1105 66-67 0.10184179852173258 1105 68-69 0.14597489343852033 1105 70-71 0.1322329258078625 1105 72-73 0.1541948944695335 1105 74-75 0.12067874516773003 1105 76-77 0.11152416257191788 1105 78-79 0.10130714761257309 1105 80-81 0.12880437509299014 1105 82-83 0.07620151902475669 1105 84-85 0.027436117000306126 1105 86-87 0.05441059207342391 1105 88-89 0.029696440938977275 1105 90-91 0.03643915239341311 1105 92-93 0.08406725964883677 1105 94-95 0.08166840833186129 1105 96-97 0.10685106752982776 1105 98-99 0.13854184100997813 1105 100 0.060629919871672655 1106 1 0.11806372299976076 1106 2 0.14016580786091737 1106 3 0.2523722998141693 1106 4 0.10426315749288761 1106 5 0.10099679945020767 1106 6 0.15512076377756756 1106 7 0.11786945145774297 1106 8 0.1116131826238913 1106 9 0.17314666740028173 1106 10-11 0.1602181037828032 1106 12-13 0.1544634345701894 1106 14-15 0.23791873679412845 1106 16-17 0.17823309394974274 1106 18-19 0.15239288723953592 1106 20-21 0.17817668813302845 1106 22-23 0.16837856706894172 1106 24-25 0.15028194162519526 1106 26-27 0.12033055526325853 1106 28-29 0.11438628096370707 1106 30-31 0.16128655146803084 1106 32-33 0.18003638650250764 1106 34-35 0.15489600883956456 1106 36-37 0.18316770894624312 1106 38-39 0.20605358088194237 1106 40-41 0.1735637075313079 1106 42-43 0.2403684516322926 1106 44-45 0.2607331678672722 1106 46-47 0.21566781467128493 1106 48-49 0.2658666577292834 1106 50-51 0.2363988338710783 1106 52-53 0.18519803145195368 1106 54-55 0.26033533900759664 1106 56-57 0.20637875507700443 1106 58-59 0.2391908535890579 1106 60-61 0.2417734431506915 1106 62-63 0.20113130247095512 1106 64-65 0.24856380854986782 1106 66-67 0.22695796179407068 1106 68-69 0.2111555706008872 1106 70-71 0.267128430211379 1106 72-73 0.278262239335227 1106 74-75 0.27105901434487834 1106 76-77 0.2602631565361264 1106 78-79 0.22814798944072834 1106 80-81 0.1965341905620548 1106 82-83 0.21632790280894199 1106 84-85 0.2530583028854352 1106 86-87 0.22904090217681983 1106 88-89 0.20603362548781945 1106 90-91 0.22348143075684135 1106 92-93 0.31149606207379676 1106 94-95 0.24755188138187378 1106 96-97 0.2816867110462091 1106 98-99 0.23717147157762142 1106 100 0.205942330843488 1107 1 0.08404327759471286 1107 2 0.08492990930414379 1107 3 0.20414915346313478 1107 4 0.09353137901815956 1107 5 0.15690629313295545 1107 6 0.13247940614938614 1107 7 0.12030180933076196 1107 8 0.12304457942893521 1107 9 0.10552257760065231 1107 10-11 0.13456434538151285 1107 12-13 0.08390335433277585 1107 14-15 0.15763915685639773 1107 16-17 0.14134590609241116 1107 18-19 0.1488003361310959 1107 20-21 0.1648255070197564 1107 22-23 0.19805042759315938 1107 24-25 0.18997980267298686 1107 26-27 0.21537915120990903 1107 28-29 0.24092287060367568 1107 30-31 0.2989829057961586 1107 32-33 0.2310545563096511 1107 34-35 0.22752144814527497 1107 36-37 0.22144330637873821 1107 38-39 0.23376865162387617 1107 40-41 0.26766596607088644 1107 42-43 0.23288085950806447 1107 44-45 0.2636764042156159 1107 46-47 0.2521058754459702 1107 48-49 0.1758522864743739 1107 50-51 0.1727744389553436 1107 52-53 0.18204087958901027 1107 54-55 0.25119265914995736 1107 56-57 0.22312818468537188 1107 58-59 0.2556801227774983 1107 60-61 0.25150270752126147 1107 62-63 0.26640356308404023 1107 64-65 0.21138841967306377 1107 66-67 0.20674178775183094 1107 68-69 0.16935683716695138 1107 70-71 0.25999897945258965 1107 72-73 0.2610107409417992 1107 74-75 0.25270139845716955 1107 76-77 0.2066887202041059 1107 78-79 0.20791612985239283 1107 80-81 0.20447769012677242 1107 82-83 0.21860346490716864 1107 84-85 0.22029173425143966 1107 86-87 0.19546921379967586 1107 88-89 0.20687847239796042 1107 90-91 0.19773493186265512 1107 92-93 0.1811434560110321 1107 94-95 0.16853823483739205 1107 96-97 0.16391282666873863 1107 98-99 0.21799634508156984 1107 100 0.1842873481578735 1108 1 0.14905742792222298 1108 2 0.16417111044460597 1108 3 0.25452258474349776 1108 4 0.17376093476608645 1108 5 0.1397356507083174 1108 6 0.14460951743216555 1108 7 0.1558796450397537 1108 8 0.12342153862165617 1108 9 0.1595489570085178 1108 10-11 0.09885205050148471 1108 12-13 0.11394109010222309 1108 14-15 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2215 9 -0.4147271755121622 2215 10-11 -0.43642029500755086 2215 12-13 -0.4940377975006669 2215 14-15 -0.583252587830188 2215 16-17 -0.5823343559553535 2215 18-19 -0.5681345502069775 2215 20-21 -0.548142297014472 2215 22-23 -0.6060762127463235 2215 24-25 -0.5589454199387305 2215 26-27 -0.5319981708196622 2215 28-29 -0.49028425082684635 2215 30-31 -0.5317022957823951 2215 32-33 -0.48970742210277507 2215 34-35 -0.5568425233241427 2215 36-37 -0.5551321086385741 2215 38-39 -0.5181062055545951 2215 40-41 -0.6504163062785793 2215 42-43 -0.5202611806501665 2215 44-45 -0.5451612859701669 2215 46-47 -0.5613270548933187 2215 48-49 -0.5551073893255065 2215 50-51 -0.5827349036668963 2215 52-53 -0.6195132308676747 2215 54-55 -0.5295141094569402 2215 56-57 -0.4275684269792279 2215 58-59 -0.4841544689489439 2215 60-61 -0.5248928556797381 2215 62-63 -0.4950368910320151 2215 64-65 -0.6199378509331623 2215 66-67 -0.6232920359605529 2215 68-69 -0.5813422874864429 2215 70-71 -0.5821557040549052 2215 72-73 -0.49880420032909 2215 74-75 -0.49133149288052635 2215 76-77 -0.5554413092079145 2215 78-79 -0.49242952338062196 2215 80-81 -0.5157017216372779 2215 82-83 -0.4928078720453186 2215 84-85 -0.416343677535842 2215 86-87 -0.43281421827735755 2215 88-89 -0.3995236737168142 2215 90-91 -0.43174139924517974 2215 92-93 -0.43831803394337854 2215 94-95 -0.4918854528407657 2215 96-97 -0.5100708145559842 2215 98-99 -0.524395263010117 2215 100 -0.5416193549549497 2216 1 -0.4513466966822435 2216 2 -0.44148706051653264 2216 3 -0.42269026537571364 2216 4 -0.4147934403033702 2216 5 -0.39773110139533685 2216 6 -0.4453685786769981 2216 7 -0.425211175685277 2216 8 -0.3646737572504932 2216 9 -0.5627453369448219 2216 10-11 -0.5270500002166472 2216 12-13 -0.5821166139850931 2216 14-15 -0.7931766500093644 2216 16-17 -0.8110106887690733 2216 18-19 -0.7840794698272688 2216 20-21 -0.7504200579203939 2216 22-23 -0.7633467442928463 2216 24-25 -0.7515976640439135 2216 26-27 -0.7954714044382385 2216 28-29 -0.7586422012605993 2216 30-31 -0.7861393790536582 2216 32-33 -0.8039191220189821 2216 34-35 -0.7914791944674917 2216 36-37 -0.8740340513744655 2216 38-39 -0.7351413567643164 2216 40-41 -0.9096623932426979 2216 42-43 -1.038354935147936 2216 44-45 -0.9764687192726598 2216 46-47 -0.9670867108214907 2216 48-49 -0.9082321188172173 2216 50-51 -0.9308179777443897 2216 52-53 -0.9594826798111527 2216 54-55 -0.936736302793804 2216 56-57 -0.8996384949834564 2216 58-59 -0.8914109393201954 2216 60-61 -0.9322411908389228 2216 62-63 -0.9982275165165433 2216 64-65 -0.9796391812931873 2216 66-67 -0.8982646365225975 2216 68-69 -0.9021571923882945 2216 70-71 -0.9433994425691736 2216 72-73 -0.9303172608018144 2216 74-75 -0.9088868398386296 2216 76-77 -0.8220913991036696 2216 78-79 -0.8318818270565984 2216 80-81 -0.8719742274008482 2216 82-83 -0.9441645442201292 2216 84-85 -1.0248300242514432 2216 86-87 -0.9155848873758181 2216 88-89 -0.8713364479068133 2216 90-91 -0.7914731643417099 2216 92-93 -0.8245711025846632 2216 94-95 -0.8265337863509146 2216 96-97 -0.8078025367682606 2216 98-99 -0.9058069662337402 2216 100 -0.9148442658825431 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 295.0 3 24.0 4 49.0 5 112.0 6 347.0 7 881.0 8 1599.0 9 2677.0 10 3766.0 11 5469.0 12 8319.0 13 12377.0 14 13269.0 15 16396.0 16 20383.0 17 29456.0 18 30062.0 19 15271.0 20 10552.0 21 9732.0 22 10084.0 23 10754.0 24 12587.0 25 15426.0 26 19264.0 27 25098.0 28 32458.0 29 42315.0 30 56542.0 31 72903.0 32 95674.0 33 125685.0 34 173772.0 35 256155.0 36 417144.0 37 809818.0 38 1247145.0 39 573560.0 40 2284.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.71081671887703 21.17413189395939 14.75261678585065 22.362434601312934 2 15.697427377632483 21.367493966079895 40.08463278739356 22.850445868894063 3 17.39551413210122 26.70354168620553 31.5108916803678 24.390052501325453 4 12.48810920581936 18.412380398229157 39.10298432616281 29.996526069788676 5 11.831432186583564 37.59432355035566 35.866135213359776 14.708109049701 6 28.304420152944097 39.23231755794738 18.403439292369757 14.059822996738758 7 24.75926524940522 33.613791790040636 23.709095189515814 17.917847771038332 8 23.944016131285853 35.67357879888145 21.673855373490564 18.708549696342132 9 24.20197698210208 16.954836993241624 23.12292927920255 35.720256745453746 10-11 22.677682438756428 27.361207875007416 29.727140965006132 20.23396872123002 12-13 23.908594962705493 25.238234573548752 29.89918664096788 20.95398382277788 14-15 21.25859869502721 27.18767884041549 28.110339870957368 23.443382593599928 16-17 19.66810329152495 30.343069748479795 28.64814111238499 21.34068584761026 18-19 19.428181995662854 28.214402263892367 32.17154851156924 20.185867228875537 20-21 20.737963740973044 27.69126713279218 31.809130503021265 19.76163862321351 22-23 20.37995992060682 27.69443721373571 31.91013047813912 20.01547238751835 24-25 20.14801765866674 27.614850238198684 31.991547726824678 20.245584376309903 26-27 19.943086878879463 27.54724975739909 32.06179193550548 20.44787142821597 28-29 19.99783955993056 27.558638123656603 32.15255195104725 20.290970365365585 30-31 20.366537917517604 28.09464497964449 31.573527694784126 19.965289408053778 32-33 19.821666797457425 27.752778665666277 32.1657586278837 20.259795908992597 34-35 20.027004304611044 27.398590905001885 32.04917142457935 20.525233365807722 36-37 20.700915662927326 27.85229528215395 31.283016692091113 20.163772362827608 38-39 20.426255064951967 27.976753856253932 31.447669500041155 20.149321578752946 40-41 20.18317565071594 27.798248392709148 31.520748837716738 20.49782711885818 42-43 20.21012732002075 27.898626314207895 31.278351289947803 20.612895075823552 44-45 20.55761604171013 28.078651024091663 30.91492842555358 20.44880450864463 46-47 20.605011742458316 28.438939695251147 30.349302247240473 20.60674631505006 48-49 20.5632384494213 28.245397281721385 30.3396963038531 20.85166796500422 50-51 20.697542218300626 28.188455450433807 30.082967597705483 21.03103473356008 52-53 20.710784782845867 28.39244118722283 29.882857733466295 21.013916296465013 54-55 20.85962307378704 28.842030440433103 29.687652522762377 20.610693963017475 56-57 20.581852207716146 28.48620380773375 29.74034764184258 21.191596342707523 58-59 20.4415551914681 28.04425863649675 30.2071988829831 21.30698728905205 60-61 20.840829876948224 28.49785535052243 29.764332593887033 20.896982178642315 62-63 20.656929294514637 28.41228709018629 29.94192890214235 20.98885471315672 64-65 20.640516649025862 28.161527706268195 30.123054168429153 21.07490147627679 66-67 20.557831367962898 28.323536786337023 29.852424956408395 21.26620688929168 68-69 21.079734354394475 28.439669411996636 29.67696994811116 20.803626285497728 70-71 21.10773872982393 28.178622218224064 29.74041941726017 20.973219634691834 72-73 20.788170645576816 28.32320183438827 29.979120531023252 20.90950698901166 74-75 20.857840650916906 28.351289947804915 29.767012209477034 21.02385719180114 76-77 20.983423706559126 28.458917186480193 29.46144033165985 21.096218775300834 78-79 21.10556154215705 28.542272371440657 29.57686716571317 20.77529892068912 80-81 20.842681104721756 28.33637382631986 29.735770193758153 21.085174875200224 82-83 20.737587831217162 28.40184956809477 29.867560387718818 20.99300221296925 84-85 20.81796335897003 28.366580647445765 29.64256166611559 21.17289432746862 86-87 20.954240173563466 28.506669706770722 29.49246252375929 21.046627595906532 88-89 20.710511416006895 28.442623411675466 29.566860329841084 21.280004842476558 90-91 20.585190802309125 28.327471041738967 29.632152417428177 21.45518573852373 92-93 20.71552324433126 28.57072277855581 29.442149687184227 21.271604289928707 94-95 20.603651918495938 28.338190348167874 29.671792919743993 21.38636481359219 96-97 20.544683172326096 28.429705401911992 29.61038261923318 21.415228806528734 98-99 20.504670236335013 28.414751439112273 29.555346607140294 21.52523171741242 100 20.694346982568355 28.382607638783387 29.442086352192515 21.480959026455746 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1734.0 1 1214.5 2 2115.5 3 3120.0 4 1731.5 5 821.5 6 1639.5 7 4123.5 8 6583.5 9 7378.5 10 5767.0 11 3551.0 12 3218.0 13 3532.5 14 3918.5 15 4473.0 16 5066.0 17 5715.5 18 6458.0 19 7441.5 20 8799.0 21 10675.0 22 13769.5 23 18680.0 24 24888.0 25 32246.0 26 41669.0 27 52902.0 28 66402.0 29 80406.5 30 92294.0 31 103679.5 32 115207.0 33 125186.5 34 132957.5 35 139113.5 36 143833.0 37 147112.5 38 150112.5 39 151034.5 40 150514.5 41 148739.5 42 144542.5 43 140449.5 44 136354.0 45 131991.0 46 128537.5 47 125223.0 48 121977.0 49 119706.5 50 116335.0 51 110053.0 52 101434.0 53 93754.5 54 88708.5 55 83764.5 56 76650.0 57 69206.5 58 63436.5 59 58053.5 60 50677.0 61 42122.0 62 34474.5 63 27681.0 64 21833.0 65 16941.5 66 12905.5 67 9721.0 68 7067.0 69 5107.5 70 3684.5 71 2574.5 72 1845.5 73 1316.0 74 958.5 75 672.5 76 387.0 77 244.0 78 165.5 79 87.0 80 46.0 81 17.5 82 10.0 83 10.5 84 5.5 85 3.0 86 2.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 1.0 98 1.0 99 0.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11785523568176119 2 0.0 3 0.0 4 0.0 5 0.011292665700729048 6 1.6747597437521893E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.1532625276813862E-4 82-83 6.100910495097261E-4 84-85 4.3065250553627724E-4 86-87 5.383156319203465E-4 88-89 5.861659103132662E-4 90-91 5.861659103132662E-4 92-93 1.7943854397344886E-4 94-95 2.5121396156282835E-4 96-97 6.340161887061859E-4 98-99 0.0010527061246442331 100 1.6747597437521893E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4179704.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.4933016500935 #Duplication Level Percentage of deduplicated Percentage of total 1 79.47534662378928 52.845781968013604 2 13.490557222916916 17.940633817025244 3 3.5318962196071975 7.045423221814988 4 1.2788431340344568 3.4013800909801635 5 0.6570396144655318 2.184436664035887 6 0.37092224834672055 1.479830696882963 7 0.23936105227868812 1.1141134656715426 8 0.16175399481149197 0.8604445736086554 9 0.12034437576980811 0.7201885391958649 >10 0.6209384982149958 7.233967395140321 >50 0.03585617211833401 1.6195747313662596 >100 0.01613276341188888 1.8510739256664046 >500 5.04040284287347E-4 0.21679610069903765 >1k 3.96031389658766E-4 0.5506560581320844 >5k 3.600285360531929E-5 0.21313324430694727 >10k+ 7.200570721063857E-5 0.7225655074600236 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15420 0.3689256464094108 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14763 0.35320682995733665 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8903 0.213005514266082 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4231 0.1012272639402216 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4228 0.10115548852263222 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 2.3925139196459845E-5 0.0 6 0.0 0.0 0.0 2.3925139196459845E-5 0.0 7 0.0 0.0 0.0 2.3925139196459845E-5 0.0 8 0.0 0.0 0.0 2.3925139196459845E-5 0.0 9 0.0 0.0 0.0 4.785027839291969E-5 0.0 10-11 0.0 0.0 0.0 4.785027839291969E-5 0.0 12-13 0.0 2.3925139196459845E-5 0.0 5.981284799114961E-5 0.0 14-15 0.0 2.3925139196459845E-5 0.0 9.570055678583938E-5 0.0 16-17 0.0 2.3925139196459845E-5 0.0 9.570055678583938E-5 0.0 18-19 0.0 2.3925139196459845E-5 0.0 9.570055678583938E-5 0.0 20-21 0.0 2.3925139196459845E-5 0.0 1.7943854397344883E-4 0.0 22-23 0.0 2.3925139196459845E-5 0.0 2.3925139196459842E-4 0.0 24-25 0.0 2.3925139196459845E-5 0.0 3.828022271433575E-4 0.0 26-27 0.0 2.3925139196459845E-5 0.0 5.622407711168064E-4 0.0 28-29 0.0 3.588770879468977E-5 0.0 0.0012201820990194521 0.0 30-31 0.0 4.785027839291969E-5 0.0 0.002954754690762791 0.0 32-33 0.0 4.785027839291969E-5 0.0 0.007129691480545034 0.0 34-35 0.0 4.785027839291969E-5 0.0 0.014642185188233426 0.0 36-37 0.0 4.785027839291969E-5 0.0 0.025217096713068675 0.0 38-39 0.0 4.785027839291969E-5 0.0 0.03820844729674637 0.0 40-41 0.0 4.785027839291969E-5 0.0 0.053257359851319616 0.0 42-43 0.0 4.785027839291969E-5 0.0 0.07047149750317247 0.0 44-45 0.0 4.785027839291969E-5 0.0 0.08926469434199169 0.0 46-47 0.0 4.785027839291969E-5 0.0 0.1114193732379135 0.0 48-49 0.0 4.785027839291969E-5 0.0 0.13326302532428136 0.0 50-51 0.0 4.785027839291969E-5 0.0 0.15666181145841906 0.0 52-53 0.0 4.785027839291969E-5 0.0 0.17965387022621698 0.0 54-55 0.0 4.785027839291969E-5 0.0 0.2078855344780396 0.0 56-57 0.0 4.785027839291969E-5 0.0 0.2393470925213843 0.0 58-59 0.0 4.785027839291969E-5 0.0 0.27367966726830417 0.0 60-61 0.0 4.785027839291969E-5 0.0 0.3088735470262966 0.0 62-63 0.0 4.785027839291969E-5 0.0 0.34413920220187844 0.0 64-65 0.0 4.785027839291969E-5 0.0 0.384907639392646 0.0 66-67 0.0 4.785027839291969E-5 0.0 0.43430108926373734 0.0 68-69 0.0 4.785027839291969E-5 0.0 0.48771396251983395 0.0 70-71 0.0 4.785027839291969E-5 0.0 0.5457563502104456 0.0 72-73 0.0 4.785027839291969E-5 0.0 0.6110360925079862 0.0 74-75 0.0 4.785027839291969E-5 0.0 0.6790193755347269 0.0 76-77 0.0 4.785027839291969E-5 0.0 0.7505675043017401 0.0 78-79 0.0 4.785027839291969E-5 0.0 0.8335877373134557 0.0 80-81 0.0 4.785027839291969E-5 0.0 0.925902886902996 0.0 82-83 0.0 4.785027839291969E-5 0.0 1.0251922145683043 0.0 84-85 0.0 4.785027839291969E-5 0.0 1.1262520025341507 0.0 86-87 0.0 4.785027839291969E-5 0.0 1.2399323014261296 0.0 88 0.0 4.785027839291969E-5 0.0 1.3345203392393337 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8650 0.0 58.49079 1 GTATCAA 15515 0.0 39.13209 1 TATAACG 505 0.0 35.37094 2 GTGGTAT 3370 0.0 34.355824 1 TGGTATC 3520 0.0 32.183167 2 ATCAACG 19255 0.0 31.0526 3 TCAACGC 19365 0.0 30.851936 4 TATCAAC 19765 0.0 30.48917 2 CAACGCA 20150 0.0 29.696669 5 ATAACGC 595 0.0 29.230698 3 AACGCAG 21090 0.0 28.41322 6 ACGCAGA 23635 0.0 25.313934 7 CGCAGAG 23650 0.0 25.238262 8 GTACCTG 3165 0.0 24.833496 1 TAACGCA 700 0.0 24.174576 4 TACCTGG 3150 0.0 23.577673 2 GCAGAGT 25735 0.0 22.919567 9 GTACATG 11810 0.0 22.316868 1 TACATGG 11790 0.0 21.848476 2 CAGAGTA 25670 0.0 20.853777 10-11 >>END_MODULE