FastQCFastQC Report
Wed 25 May 2016
SRR2049443_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049443_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6466457
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66950.043.130781
GTATCAA115850.035.277131
ATCAACG121350.032.1491363
TCAACGC121650.032.1074944
CAACGCA133300.029.2654615
TATCAAC139300.028.4113542
AACGCAG143450.027.3260146
ACGCAGA158600.024.3306737
CGCAGAG158700.023.8100198
GTGGTAT38950.022.4582041
GTACATG184800.021.1988641
TACATGG180300.020.7514442
GCAGAGT183150.019.9132469
ACATGGG184250.019.337163
TGGTATC41950.018.3756562
AGAGTAC159700.017.2752310-11
CAGAGTA174800.017.00629610-11
CATGGGG126850.016.8222054
TCTGTCG15100.015.873548
CTGTCGC16200.015.6662859
Produced by FastQC (version 0.11.5)