##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049443_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6466457
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.95335575570981	31.0	31.0	34.0	28.0	34.0
2	31.204689677825122	31.0	31.0	34.0	28.0	34.0
3	31.274362761555516	33.0	31.0	34.0	28.0	34.0
4	34.890146953733705	37.0	35.0	37.0	32.0	37.0
5	34.908007584369614	37.0	35.0	37.0	32.0	37.0
6	34.90017501082896	37.0	35.0	37.0	32.0	37.0
7	34.82903821984744	37.0	35.0	37.0	32.0	37.0
8	34.79917859192445	37.0	35.0	37.0	32.0	37.0
9	36.37537031484165	39.0	37.0	39.0	32.0	39.0
10-11	36.32859686533135	39.0	37.0	39.0	32.0	39.0
12-13	36.26460208426346	39.0	37.0	39.0	32.0	39.0
14-15	37.49924904781707	40.0	38.0	41.0	32.0	41.0
16-17	37.48291738428014	40.0	38.0	41.0	32.0	41.0
18-19	37.490893003695845	40.0	38.0	41.0	32.0	41.0
20-21	37.54903133199525	40.0	38.0	41.0	32.0	41.0
22-23	37.50040145631526	40.0	38.0	41.0	32.0	41.0
24-25	37.50020150137858	40.0	38.0	41.0	32.0	41.0
26-27	37.503866027408826	40.0	38.0	41.0	32.0	41.0
28-29	37.48244347716222	40.0	38.0	41.0	32.0	41.0
30-31	37.423406279512875	40.0	38.0	41.0	32.0	41.0
32-33	37.385114367883375	40.0	38.0	41.0	32.0	41.0
34-35	37.318390271519625	40.0	38.0	41.0	31.5	41.0
36-37	37.20358489973721	40.0	37.0	41.0	31.0	41.0
38-39	37.09343826147765	40.0	37.0	41.0	31.0	41.0
40-41	36.974861581852316	40.0	37.0	41.0	30.0	41.0
42-43	36.87271074407516	40.0	37.0	41.0	30.0	41.0
44-45	36.70326726057252	40.0	37.0	41.0	30.0	41.0
46-47	36.58383849146449	40.0	36.0	41.0	30.0	41.0
48-49	36.485483549956335	40.0	36.0	41.0	30.0	41.0
50-51	36.37130827592297	40.0	36.0	41.0	30.0	41.0
52-53	36.25985729124929	39.5	36.0	41.0	30.0	41.0
54-55	36.11500076471552	39.0	35.0	41.0	29.0	41.0
56-57	35.95074335142104	39.0	35.0	41.0	29.0	41.0
58-59	35.76822083561369	39.0	35.0	41.0	29.0	41.0
60-61	35.573823579125325	39.0	35.0	40.5	28.0	41.0
62-63	35.322860493775806	38.0	35.0	40.0	28.0	41.0
64-65	35.08623949714658	38.0	34.0	40.0	28.0	41.0
66-67	34.86371934430245	37.5	34.0	40.0	28.0	41.0
68-69	34.583360176987185	37.0	34.0	40.0	28.0	41.0
70-71	34.27144663607908	36.5	34.0	39.5	27.0	41.0
72-73	33.93414771025308	36.0	34.0	39.0	26.0	41.0
74-75	33.63263151676412	36.0	34.0	39.0	26.0	40.5
76-77	33.30381366488635	35.0	33.0	38.5	26.0	40.0
78-79	32.95278952601092	35.0	33.0	37.0	26.0	39.0
80-81	32.60768810493907	35.0	33.0	37.0	25.5	39.0
82-83	32.295593553007464	35.0	33.0	36.5	25.0	39.0
84-85	31.994395153327396	35.0	33.0	36.0	25.0	37.5
86-87	31.710201737984185	35.0	33.0	36.0	24.0	37.0
88-89	31.465283384703554	35.0	32.5	35.0	24.0	37.0
90-91	31.23906367892031	35.0	32.0	35.0	23.0	36.0
92-93	31.013061789477607	35.0	32.0	35.0	20.5	36.0
94-95	30.786396940395644	35.0	32.0	35.0	19.5	36.0
96-97	30.580192754703234	35.0	32.0	35.0	17.5	36.0
98-99	30.382925379384723	35.0	32.0	35.0	4.5	35.0
100	30.233619739526606	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.22675835419834556
1101	2	0.23407825725415776
1101	3	0.299216918683495
1101	4	0.3015759840123877
1101	5	0.2919101760761649
1101	6	0.3070738906554027
1101	7	0.35653495380611133
1101	8	0.3042325546555631
1101	9	0.4004496753411857
1101	10-11	0.3947319352556846
1101	12-13	0.426544873231002
1101	14-15	0.5253023647903987
1101	16-17	0.4436623199307874
1101	18-19	0.4589478940216978
1101	20-21	0.4494274563641909
1101	22-23	0.46338945090457884
1101	24-25	0.49860209990755067
1101	26-27	0.4612959858991985
1101	28-29	0.4679711883710169
1101	30-31	0.3704944623891535
1101	32-33	0.423564610727027
1101	34-35	0.4387518444792988
1101	36-37	0.4313866227267198
1101	38-39	0.42801388729833434
1101	40-41	0.42783491113356575
1101	42-43	0.3871118178748887
1101	44-45	0.3400061770806033
1101	46-47	0.3035007954046378
1101	48-49	0.3405858314416861
1101	50-51	0.29678079221956466
1101	52-53	0.28263880681207354
1101	54-55	0.26594504442918065
1101	56-57	0.2316415923784163
1101	58-59	0.23759004263584416
1101	60-61	0.2844680686894492
1101	62-63	0.23898115299882505
1101	64-65	0.24818722227484358
1101	66-67	0.23086702755752242
1101	68-69	0.1986013820511161
1101	70-71	0.2237975076836065
1101	72-73	0.29760509525335976
1101	74-75	0.22992918104387883
1101	76-77	0.1556424711221709
1101	78-79	0.13112407057663944
1101	80-81	0.17477239310665027
1101	82-83	0.11404523683194157
1101	84-85	0.1638412652188137
1101	86-87	0.11674426792425763
1101	88-89	0.07045908746957608
1101	90-91	0.05987346755295775
1101	92-93	0.043446578764601895
1101	94-95	0.04265743913223474
1101	96-97	0.002096738111827534
1101	98-99	0.06919929283489168
1101	100	0.0272880892495877
1102	1	0.11241090022884137
1102	2	0.15013612126708864
1102	3	0.20958618993691047
1102	4	0.19000139132624128
1102	5	0.15422458122641558
1102	6	0.15679724626079405
1102	7	0.2064186778903263
1102	8	0.24291735230055167
1102	9	0.25054222269776005
1102	10-11	0.27848586529018604
1102	12-13	0.3052446370418096
1102	14-15	0.3732739302185024
1102	16-17	0.3132415795540098
1102	18-19	0.35658692186629537
1102	20-21	0.3795634189559465
1102	22-23	0.32685761275367753
1102	24-25	0.26541369187381747
1102	26-27	0.21282452678585173
1102	28-29	0.3033160711447067
1102	30-31	0.24224271056000646
1102	32-33	0.248115980394644
1102	34-35	0.2995605201684839
1102	36-37	0.26239260607531634
1102	38-39	0.3172547381239781
1102	40-41	0.34688463780695855
1102	42-43	0.28909961147585506
1102	44-45	0.2574301934690766
1102	46-47	0.1763051047583275
1102	48-49	0.16436369112928162
1102	50-51	0.2113302442205338
1102	52-53	0.16676096729622003
1102	54-55	0.18308105357910875
1102	56-57	0.17759665506513755
1102	58-59	0.20381818822491482
1102	60-61	0.1387460125039226
1102	62-63	0.12740823609508567
1102	64-65	0.09577368900949068
1102	66-67	0.13026396413375352
1102	68-69	0.10599051462560993
1102	70-71	0.04775403955085977
1102	72-73	0.09214804106163399
1102	74-75	0.0583809147693799
1102	76-77	0.02352046086087256
1102	78-79	0.07608749227384948
1102	80-81	0.05631660268867478
1102	82-83	0.03220077121115139
1102	84-85	-0.006033556953390473
1102	86-87	-0.003218960213587252
1102	88-89	-0.04061207550054746
1102	90-91	-0.11449655647750134
1102	92-93	-0.14001445438409021
1102	94-95	-0.09841741263766934
1102	96-97	-0.12761317174303244
1102	98-99	-0.14270487948396848
1102	100	-0.13299274226272928
1103	1	0.07125988845255549
1103	2	0.12492008678243494
1103	3	0.14174735579477016
1103	4	0.15474902622404585
1103	5	0.18967226477050048
1103	6	0.18431320246942562
1103	7	0.20736073510386888
1103	8	0.18355247465898117
1103	9	0.19209690745171315
1103	10-11	0.22556548668951137
1103	12-13	0.2409994491739056
1103	14-15	0.2733146884504407
1103	16-17	0.2805526203575326
1103	18-19	0.2631090862483134
1103	20-21	0.23329461187596934
1103	22-23	0.23942461535286697
1103	24-25	0.25119157853620067
1103	26-27	0.24924576152739775
1103	28-29	0.24314232146764425
1103	30-31	0.2105223973033148
1103	32-33	0.2839624328974324
1103	34-35	0.23775506006175107
1103	36-37	0.23858153152956874
1103	38-39	0.235869911047196
1103	40-41	0.25572993098430175
1103	42-43	0.20984067476528168
1103	44-45	0.20187733277730757
1103	46-47	0.15103488947301003
1103	48-49	0.16046150290869576
1103	50-51	0.18156703587283118
1103	52-53	0.18722980860400895
1103	54-55	0.0758622720015083
1103	56-57	0.09714102128751989
1103	58-59	0.10941900028784346
1103	60-61	0.09006269886263851
1103	62-63	0.1349190279097101
1103	64-65	0.16708498107121272
1103	66-67	0.060628133436303244
1103	68-69	0.07210840104819027
1103	70-71	0.061841239721943
1103	72-73	0.06526192737044312
1103	74-75	0.06539042269474038
1103	76-77	-0.018912346770875388
1103	78-79	0.09116770364189364
1103	80-81	0.025721909810819454
1103	82-83	0.0016779477904123041
1103	84-85	-0.08282135862797446
1103	86-87	-0.035613696852283994
1103	88-89	-0.03987682568469353
1103	90-91	-0.08550579614967901
1103	92-93	-0.06783741531059206
1103	94-95	0.015079888182363277
1103	96-97	0.025515309937024
1103	98-99	-0.012942883824972995
1103	100	-0.026648700669316128
1104	1	0.09618442039180763
1104	2	0.0986083693325952
1104	3	0.12332188070796946
1104	4	0.12809209358465523
1104	5	0.16014639834884292
1104	6	0.1617395679342195
1104	7	0.13249255279237104
1104	8	0.1312098326587403
1104	9	0.1823467498846867
1104	10-11	0.26890927576013723
1104	12-13	0.24784238625411348
1104	14-15	0.33648156268686336
1104	16-17	0.25672857753166767
1104	18-19	0.2513251885362493
1104	20-21	0.3394557287840456
1104	22-23	0.3346925091986961
1104	24-25	0.33166084525912964
1104	26-27	0.3204046249012862
1104	28-29	0.2443468604706851
1104	30-31	0.2710494350026593
1104	32-33	0.23628009509238268
1104	34-35	0.2740961972218514
1104	36-37	0.2655126771899958
1104	38-39	0.2558258990771094
1104	40-41	0.2546323847200469
1104	42-43	0.23883901174699673
1104	44-45	0.2485734067341383
1104	46-47	0.21747144115915518
1104	48-49	0.17348342046587106
1104	50-51	0.12233709452831931
1104	52-53	0.12589407477597803
1104	54-55	0.08712972935636287
1104	56-57	0.10629281337465812
1104	58-59	0.11653044861887452
1104	60-61	0.10949605824571762
1104	62-63	0.18501128029689795
1104	64-65	0.16205315997233072
1104	66-67	0.11048550967696968
1104	68-69	0.13724698420445236
1104	70-71	0.08562945217295237
1104	72-73	0.1244380667876328
1104	74-75	0.14496581679266995
1104	76-77	0.09160254930252876
1104	78-79	0.06735146292614758
1104	80-81	0.1342123570733449
1104	82-83	0.12856015790428899
1104	84-85	0.12121076238046413
1104	86-87	0.08354728404394862
1104	88-89	0.07265118685715777
1104	90-91	0.05748513700333646
1104	92-93	0.020221382198059246
1104	94-95	0.08241810867044563
1104	96-97	0.11405874011295225
1104	98-99	0.13518436530384648
1104	100	0.09775158405608408
1105	1	0.06645164656381297
1105	2	0.11190101421434662
1105	3	0.1100760324074237
1105	4	0.07787415603089443
1105	5	0.05642407850961462
1105	6	0.11149191466481057
1105	7	0.11287594994606565
1105	8	0.08166739456774508
1105	9	0.1873216143589076
1105	10-11	0.2171998928278427
1105	12-13	0.1757624435841194
1105	14-15	0.2294479197104735
1105	16-17	0.17367863609771916
1105	18-19	0.16528760409909893
1105	20-21	0.1743331470336713
1105	22-23	0.17158024665022964
1105	24-25	0.13467475377922966
1105	26-27	0.20515388902334308
1105	28-29	0.15246180730098047
1105	30-31	0.13899806864343134
1105	32-33	0.14482735454689788
1105	34-35	0.094361848007253
1105	36-37	0.15561405360152492
1105	38-39	0.14511514044328777
1105	40-41	0.13654793211176752
1105	42-43	0.10175581066938122
1105	44-45	0.09833426440863491
1105	46-47	0.068693710924002
1105	48-49	0.03668994761581246
1105	50-51	0.0057076628631591575
1105	52-53	0.021609702590673407
1105	54-55	0.0012146315112460115
1105	56-57	-0.030794374372426603
1105	58-59	-0.018569404238427012
1105	60-61	-0.03251417294322323
1105	62-63	0.0036136562793203097
1105	64-65	-0.06535324987086
1105	66-67	-0.0501818243154446
1105	68-69	-0.04924225189001419
1105	70-71	-0.024550126701818442
1105	72-73	-0.06267326554551289
1105	74-75	-0.0264881405092936
1105	76-77	-0.04734464943211947
1105	78-79	-0.03762626650600254
1105	80-81	-0.023431941591198324
1105	82-83	-0.031898752100119054
1105	84-85	-0.11680835444261106
1105	86-87	-0.10776810751493215
1105	88-89	-0.07073366271451675
1105	90-91	-0.07353988596566197
1105	92-93	-0.09113559117418646
1105	94-95	-0.0797007699800325
1105	96-97	-0.08017871004999222
1105	98-99	-0.060101357766964014
1105	100	-0.14089437558085294
1106	1	0.11920729904680627
1106	2	0.1574873700906032
1106	3	0.14798294057087702
1106	4	0.16015097477773566
1106	5	0.19940427042821085
1106	6	0.20528034799562533
1106	7	0.25031039582924564
1106	8	0.1942451905638265
1106	9	0.29340798187641326
1106	10-11	0.22280178660834338
1106	12-13	0.18962468463561066
1106	14-15	0.3005849832416132
1106	16-17	0.22274811239908132
1106	18-19	0.27182690476869453
1106	20-21	0.2405321356906427
1106	22-23	0.2665351421504525
1106	24-25	0.30814382105112514
1106	26-27	0.296737113249975
1106	28-29	0.2650519707620731
1106	30-31	0.23650489362763238
1106	32-33	0.24474603723594157
1106	34-35	0.28950874444296204
1106	36-37	0.254482397246214
1106	38-39	0.2530365012318043
1106	40-41	0.3092465418055852
1106	42-43	0.24144904162435665
1106	44-45	0.1518633011634094
1106	46-47	0.17646025725988324
1106	48-49	0.15129209287356105
1106	50-51	0.1624916274821402
1106	52-53	0.14125353641615135
1106	54-55	0.14726163259590663
1106	56-57	0.05578164822198772
1106	58-59	0.027848793340218947
1106	60-61	0.09667939145825954
1106	62-63	0.15521392762314434
1106	64-65	0.10025230467928026
1106	66-67	0.1651136819822554
1106	68-69	0.12828675109857812
1106	70-71	0.1868140928164621
1106	72-73	0.11538700879401631
1106	74-75	0.1413112112236874
1106	76-77	0.11107990173646698
1106	78-79	0.07795959729256907
1106	80-81	0.12034603645397368
1106	82-83	0.18238351208798065
1106	84-85	0.12960029752795066
1106	86-87	0.09316901762528929
1106	88-89	0.09023876643296092
1106	90-91	0.1361088979035543
1106	92-93	0.06233882888944464
1106	94-95	0.11897872766129325
1106	96-97	0.1709284974923868
1106	98-99	0.19721241704020542
1106	100	0.19077302472492974
1107	1	0.08224512591945299
1107	2	0.05332424935825131
1107	3	0.111222358708428
1107	4	0.06787332800444545
1107	5	0.10083509279303371
1107	6	0.11645210349909263
1107	7	0.07865693264363927
1107	8	0.09549861558151918
1107	9	0.07313749768917432
1107	10-11	0.11753272100668255
1107	12-13	0.10871299934195378
1107	14-15	0.14047075299356493
1107	16-17	0.12092621399683878
1107	18-19	0.09660241826129123
1107	20-21	0.13981412186129205
1107	22-23	0.10767177664428829
1107	24-25	0.12987475138182702
1107	26-27	0.18788936574397042
1107	28-29	0.13279193110184195
1107	30-31	0.08715986980170243
1107	32-33	0.059853919652482546
1107	34-35	0.05920227042597759
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2216	46-47	-0.1468422385241226
2216	48-49	-0.2242135332326285
2216	50-51	-0.13970732691015542
2216	52-53	-0.2545142658551498
2216	54-55	-0.21544826713437004
2216	56-57	-0.16749251331335557
2216	58-59	-0.21821446161429492
2216	60-61	-0.21192854312456433
2216	62-63	-0.22718305500792013
2216	64-65	-0.17323905035579656
2216	66-67	-0.165462916874084
2216	68-69	-0.13820721265118863
2216	70-71	-0.08382603321987858
2216	72-73	-0.11279370107342856
2216	74-75	-0.11942188794156294
2216	76-77	-0.13670657326179025
2216	78-79	-0.1453047733801256
2216	80-81	-0.12086272821341737
2216	82-83	-0.06939196351093813
2216	84-85	-0.004865322266951466
2216	86-87	-0.11740078010846489
2216	88-89	-0.11637622818008708
2216	90-91	-0.1330834783833943
2216	92-93	-0.08200895970738031
2216	94-95	-0.05263664165681803
2216	96-97	-0.08727889769572172
2216	98-99	-0.1543373946930764
2216	100	-0.25543033267756243
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	91988.0
3	16818.0
4	10421.0
5	8688.0
6	7509.0
7	7916.0
8	9073.0
9	10784.0
10	12028.0
11	13500.0
12	14917.0
13	16393.0
14	18468.0
15	19571.0
16	19177.0
17	19677.0
18	18950.0
19	17984.0
20	18102.0
21	19229.0
22	21780.0
23	25668.0
24	31069.0
25	39053.0
26	47503.0
27	55690.0
28	67709.0
29	85380.0
30	104476.0
31	123636.0
32	158391.0
33	211889.0
34	293998.0
35	432188.0
36	729708.0
37	1300091.0
38	1729056.0
39	636649.0
40	1330.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.04234769664968	20.984803016800964	15.068110478604218	22.904738807945137
2	15.159747971656865	20.658971402906015	40.645292904534166	23.535987720902956
3	17.984642761302357	25.762158458429585	30.684363039616542	25.56883574065152
4	12.655853512816034	17.594339542393037	39.30681387065139	30.44299307413954
5	13.107992239871127	37.854894127195834	35.73485525231555	13.302258380617493
6	29.908621669585795	38.4187380866768	18.29450314917704	13.378137094560373
7	26.300381246767262	32.77116655818684	23.151429582571293	17.777022612474603
8	24.368867382346227	36.22654518302041	21.162476128376902	18.242111306256454
9	24.475168651740322	17.523541801767724	22.24026920558956	35.76102034090239
10-11	23.27584160254974	27.41791882620575	29.428877907862024	19.87736166338249
12-13	23.833216314373736	24.991723347846058	29.32169036574489	21.853369972035317
14-15	21.38644995385738	27.331717864841025	26.52107860051226	24.76075358078933
16-17	20.918861680616992	30.4726793774363	27.18850838655136	21.41995055539535
18-19	20.863398753460462	29.46579749145517	29.077846858132666	20.5929568969517
20-21	21.995341962542213	28.66006140034832	28.566667107461125	20.777929529648336
22-23	21.707292892551298	28.55761805341494	28.541269917620554	21.193819136413204
24-25	21.488525251947195	28.71453666579407	28.49533883673729	21.301599245521444
26-27	21.27095948149327	28.72638822710481	28.57860224088466	21.42405005051726
28-29	21.22738925170226	28.744346104621716	28.710683725983756	21.317580917692275
30-31	21.580901372682906	28.991912066633617	28.31340413142679	21.11378242925668
32-33	21.22453647906671	28.82357549241209	28.667759674148595	21.284128354372605
34-35	21.459741125424294	28.536489081713256	28.634588758129702	21.369181034732744
36-37	21.677373655278917	28.79448265622505	28.245826014367147	21.282317674128887
38-39	21.428873044023792	28.88020545735266	28.399005071161138	21.29191642746241
40-41	21.4444638033392	28.59779452438173	28.52318415822718	21.43455751405189
42-43	21.37644924283391	28.619185562084404	28.482089824966117	21.522275370115572
44-45	21.462198012319334	28.763203731777416	28.352314410723505	21.42228384517974
46-47	21.522326518282593	28.765025136696202	28.265980165651776	21.44666817936943
48-49	21.395025679150482	28.642903936876827	28.31445643097819	21.647613952994497
50-51	21.38941688306265	28.610196465386178	28.283823891044683	21.716562760506484
52-53	21.393716523781183	28.655712903493217	28.256596570500964	21.693974002224632
54-55	21.517237112764278	28.868970103778402	28.14226611194457	21.471526671512752
56-57	21.410264907550992	28.6316253666124	28.191988234683603	21.766121491153005
58-59	21.40076584435028	28.374059957066816	28.396970954299093	21.828203244283813
60-61	21.47770044583256	28.692965542412864	28.13592035561439	21.69341365614018
62-63	21.356145698336743	28.695691446260202	28.2411052857802	21.70705756962286
64-65	21.449072996664338	28.455120249367482	28.225941986313185	21.869864767654988
66-67	21.26556993682706	28.490306754918542	28.226100190470007	22.01802311778439
68-69	21.596274701696576	28.610402330206103	28.139875779567696	21.653447188529622
70-71	21.54694748135637	28.412857982347195	28.150878084719906	21.889316451576526
72-73	21.37282120398455	28.492506215464218	28.30326778703179	21.831404793519443
74-75	21.41494551435191	28.48334680825361	28.19790638872085	21.903801288673627
76-77	21.478482751890343	28.482369108533646	27.998416907232794	22.040731232343223
78-79	21.55350941472584	28.479874328033283	28.15827064634081	21.80834561090007
80-81	21.41671772263716	28.41609298626307	28.20939944412538	21.95778984697439
82-83	21.32993156456205	28.463546467674743	28.3486006678982	21.85792129986501
84-85	21.363695662479117	28.25819811422088	28.244054268135617	22.134051955164395
86-87	21.534780149439268	28.421324444126856	28.085953021397543	21.95794238503634
88-89	21.414119948149224	28.292552892849205	28.123941976748196	22.16938518225337
90-91	21.35076020214314	28.195529802131137	28.205807559093042	22.24790243663268
92-93	21.437659165894736	28.3411315100702	28.106107477581162	22.115101846453904
94-95	21.345703314408603	28.130287248951618	28.23695443942923	22.28705499721055
96-97	21.383094311024156	28.18066451939696	28.167262772452567	22.268978397126318
98-99	21.373614582910434	28.17562439223374	28.095726931947578	22.355034092908248
100	21.50033158139581	28.064034801199632	28.07507720402649	22.36055641337807
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1899.0
1	1378.5
2	1003.0
3	1192.0
4	1193.0
5	1102.0
6	1005.0
7	995.0
8	1025.5
9	1041.0
10	984.0
11	908.5
12	939.5
13	1009.0
14	1167.0
15	1380.5
16	1613.0
17	1991.0
18	2676.5
19	3762.0
20	5380.5
21	7845.0
22	11542.0
23	17318.5
24	25162.0
25	35102.0
26	48585.5
27	64785.5
28	85147.5
29	107631.5
30	127377.0
31	146551.5
32	165406.5
33	181122.5
34	194432.0
35	206116.5
36	215747.5
37	225194.5
38	233020.0
39	235491.0
40	235732.5
41	234096.0
42	229056.0
43	223556.5
44	218006.5
45	213608.0
46	209787.0
47	203595.0
48	198060.0
49	194527.0
50	189245.0
51	179774.5
52	168036.5
53	159785.5
54	155617.5
55	149869.5
56	137975.0
57	122796.5
58	109932.0
59	99588.5
60	87508.5
61	74597.5
62	62976.5
63	51980.5
64	42125.5
65	34050.5
66	27549.0
67	21675.0
68	16679.5
69	12683.5
70	9435.5
71	6963.0
72	5293.5
73	3910.5
74	2866.5
75	2100.5
76	1406.0
77	1005.0
78	730.0
79	508.5
80	347.5
81	230.0
82	175.0
83	129.0
84	98.0
85	82.5
86	63.0
87	50.5
88	39.5
89	33.0
90	29.5
91	25.5
92	22.0
93	20.5
94	17.5
95	11.0
96	9.5
97	16.0
98	18.5
99	17.0
100	33.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0779870646321471
2	0.012912789801277578
3	0.017041789653901667
4	0.010670449057343148
5	0.009046685070356147
6	0.007453849921216517
7	0.02092335880374678
8	0.009603404151608833
9	0.009557010894837775
10-11	0.013307132483831563
12-13	0.01516286275467385
14-15	0.009804441597616748
16-17	0.012951450848586793
18-19	0.008613681340492948
20-21	0.013276203645984194
22-23	0.013732404004232921
24-25	0.01646187394426345
26-27	0.005412546623290003
28-29	0.012093175598322233
30-31	0.010082801138243091
32-33	0.012533911537647277
34-35	0.009038952860894303
36-37	0.010276106374789162
38-39	0.01965527645200455
40-41	0.013415383416297364
42-43	0.008312125171481076
44-45	0.020629534844196752
46-47	0.01868101805981235
48-49	0.018441319566495223
50-51	0.010624055800572092
52-53	0.01040755393564049
54-55	0.01170656512523009
56-57	0.02091562659428494
58-59	0.0020103744600791437
60-61	0.007183222590052017
62-63	0.015711849626464693
64-65	0.02506009086583271
66-67	0.013484973301453948
68-69	0.014451499484184307
70-71	0.025485362386234067
72-73	0.020606338215811225
74-75	0.02103160973621258
76-77	0.00978124496923122
78-79	0.011366347908909006
80-81	0.02056767716850201
82-83	0.012386999557872263
84-85	0.011559653145455078
86-87	0.016825287788970068
88-89	0.015155130545212007
90-91	0.016059799052247622
92-93	0.00808015888762579
94-95	0.015773707302159434
96-97	0.013639617490690807
98-99	0.01035342846940759
100	0.007314670150903346
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6466457.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.75362203948228
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.02349490016496	46.94726170185296
2	14.436030886393883	17.829543902173207
3	4.481254610646651	8.302011104656819
4	1.888683972298122	4.66532304709305
5	0.9485935967588314	2.9289545221658972
6	0.5616849041235951	2.0811646364718803
7	0.3352724516649172	1.4493001782256123
8	0.25473404126272003	1.2584599763782318
9	0.16849222133069988	0.9364504457383951
>10	0.8345647119439745	9.005297591579588
>50	0.046184831762906706	1.9693902888919896
>100	0.020231765003725373	2.237828727116213
>500	6.517742841658795E-4	0.2722213943015985
>1k	1.253323608815298E-4	0.11679248335446406
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	1.5464418923685722E-5	0.0	4.639325677105716E-5	0.0
4	0.0	1.5464418923685722E-5	0.0	6.185767569474289E-5	0.0
5	0.0	1.5464418923685722E-5	0.0	6.185767569474289E-5	0.0
6	0.0	1.5464418923685722E-5	0.0	6.185767569474289E-5	0.0
7	0.0	1.5464418923685722E-5	0.0	7.732209461842861E-5	0.0
8	0.0	1.5464418923685722E-5	0.0	9.278651354211433E-5	0.0
9	0.0	1.5464418923685722E-5	0.0	1.391797703131715E-4	0.0
10-11	0.0	1.5464418923685722E-5	0.0	1.391797703131715E-4	0.0
12-13	1.5464418923685722E-5	2.319662838552858E-5	0.0	1.5464418923685723E-4	0.0
14-15	1.5464418923685722E-5	3.0928837847371444E-5	0.0	1.778408176223858E-4	0.0
16-17	3.0928837847371444E-5	3.0928837847371444E-5	0.0	2.0103744600791438E-4	0.0
18-19	3.0928837847371444E-5	4.639325677105716E-5	0.0	2.3196628385528583E-4	0.0
20-21	3.0928837847371444E-5	4.639325677105716E-5	0.0	2.6289512170265725E-4	0.0
22-23	6.185767569474289E-5	4.639325677105716E-5	0.0	4.1753931093951445E-4	0.0
24-25	7.732209461842861E-5	4.639325677105716E-5	0.0	6.804344326421718E-4	0.0
26-27	7.732209461842861E-5	4.639325677105716E-5	0.0	0.001221689094971172	0.0
28-29	7.732209461842861E-5	4.639325677105716E-5	0.0	0.0032861890212832158	0.0
30-31	7.732209461842861E-5	4.639325677105716E-5	0.0	0.008922969718966662	0.0
32-33	7.732209461842861E-5	4.639325677105716E-5	0.0	0.022477532905577198	0.0
34-35	7.732209461842861E-5	4.639325677105716E-5	0.0	0.04618448711558741	0.0
36-37	7.732209461842861E-5	4.639325677105716E-5	0.0	0.08295887531611205	0.0
38-39	7.732209461842861E-5	4.639325677105716E-5	0.0	0.13099909270254173	0.0
40-41	7.732209461842861E-5	4.639325677105716E-5	0.0	0.18037698232587027	0.0
42-43	7.732209461842861E-5	4.639325677105716E-5	0.0	0.23056675394269227	0.0
44-45	7.732209461842861E-5	4.639325677105716E-5	0.0	0.29797615603103833	0.0
46-47	8.505430408027148E-5	4.639325677105716E-5	0.0	0.3826206530098321	0.0
48-49	9.278651354211433E-5	4.639325677105716E-5	0.0	0.47218283520635795	0.0
50-51	9.278651354211433E-5	4.639325677105716E-5	0.0	0.5612114949500167	0.0
52-53	9.278651354211433E-5	6.185767569474289E-5	0.0	0.6505881041194583	0.0
54-55	9.278651354211433E-5	6.185767569474289E-5	0.0	0.7547796266177909	0.0
56-57	9.278651354211433E-5	6.185767569474289E-5	0.0	0.8816961127244796	0.0
58-59	9.278651354211433E-5	6.185767569474289E-5	0.0	1.0241543398494724	0.0
60-61	9.278651354211433E-5	7.732209461842861E-5	0.0	1.1713833402124223	0.0
62-63	1.0051872300395719E-4	7.732209461842861E-5	0.0	1.320746430386841	0.0
64-65	1.1598314192764291E-4	7.732209461842861E-5	0.0	1.4857517803025675	0.0
66-67	1.2371535138948578E-4	7.732209461842861E-5	0.0	1.6770621068074836	0.0
68-69	1.2371535138948578E-4	7.732209461842861E-5	0.0	1.8923500148535743	0.0
70-71	1.3144756085132863E-4	7.732209461842861E-5	0.0	2.1229940908908853	0.0
72-73	1.391797703131715E-4	7.732209461842861E-5	0.0	2.3648885316951773	0.0
74-75	1.391797703131715E-4	1.0825093246580005E-4	0.0	2.611631067832045	0.0
76-77	1.4691197977501438E-4	1.0825093246580005E-4	0.0	2.8827300637737174	0.0
78-79	1.5464418923685723E-4	1.2371535138948578E-4	0.0	3.186111034218584	0.0
80-81	1.5464418923685723E-4	1.2371535138948578E-4	0.0	3.516036679745957	0.0
82-83	1.5464418923685723E-4	1.2371535138948578E-4	0.0	3.858395718087973	0.0
84-85	1.6237639869870008E-4	1.2371535138948578E-4	0.0	4.208409643797214	0.0
86-87	1.7010860816054293E-4	1.2371535138948578E-4	0.0	4.581148533114811	0.0
88	1.7010860816054293E-4	1.2371535138948578E-4	0.0	4.883508851910714	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6695	0.0	43.13078	1
GTATCAA	11585	0.0	35.27713	1
ATCAACG	12135	0.0	32.149136	3
TCAACGC	12165	0.0	32.107494	4
CAACGCA	13330	0.0	29.265461	5
TATCAAC	13930	0.0	28.411354	2
AACGCAG	14345	0.0	27.326014	6
ACGCAGA	15860	0.0	24.330673	7
CGCAGAG	15870	0.0	23.810019	8
GTGGTAT	3895	0.0	22.458204	1
GTACATG	18480	0.0	21.198864	1
TACATGG	18030	0.0	20.751444	2
GCAGAGT	18315	0.0	19.913246	9
ACATGGG	18425	0.0	19.33716	3
TGGTATC	4195	0.0	18.375656	2
AGAGTAC	15970	0.0	17.27523	10-11
CAGAGTA	17480	0.0	17.006296	10-11
CATGGGG	12685	0.0	16.822205	4
TCTGTCG	1510	0.0	15.87354	8
CTGTCGC	1620	0.0	15.666285	9
>>END_MODULE