FastQCFastQC Report
Wed 25 May 2016
SRR2049443_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049443_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6466457
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174180.2693592488127579No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170030.2629415149594283No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91360.14128293128679276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114150.056.6836361
GTATCAA207600.040.2348061
ATCAACG251850.032.7727433
TCAACGC256050.032.2718854
TATCAAC264450.031.3178942
GTGGTAT48950.031.0513671
CAACGCA269050.030.7300365
AACGCAG281050.029.498616
TGGTATC49850.028.9469912
ACGCAGA316450.026.0650527
CGCAGAG315700.025.9632158
GCAGAGT350700.023.1576549
GAGTACT249750.021.12357512-13
GTACATG174300.021.112531
CAGAGTA341900.020.74324410-11
AGAGTAC325200.020.38492410-11
TACATGG174350.019.9498672
ACATGGG174450.019.6420483
GTACTTT274450.019.1796814-15
AGTACTT264200.018.81196412-13
Produced by FastQC (version 0.11.5)