##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049443_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6466457
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.840611017748977	31.0	31.0	34.0	30.0	34.0
2	32.11208456191698	33.0	31.0	34.0	30.0	34.0
3	32.096439982512834	34.0	31.0	34.0	30.0	34.0
4	35.801037724367454	37.0	35.0	37.0	33.0	37.0
5	35.870175275270526	37.0	35.0	37.0	35.0	37.0
6	35.949265416904495	37.0	35.0	37.0	35.0	37.0
7	35.920124575173084	37.0	35.0	37.0	35.0	37.0
8	35.923388804719494	37.0	35.0	37.0	35.0	37.0
9	37.64382365799386	39.0	37.0	39.0	35.0	39.0
10-11	37.61757698226401	39.0	37.0	39.0	35.0	39.0
12-13	37.602631858527786	39.0	37.0	39.0	35.0	39.0
14-15	38.89952875276214	40.0	38.0	41.0	35.0	41.0
16-17	38.90325498182389	40.0	38.0	41.0	35.0	41.0
18-19	38.98294181806204	40.0	38.5	41.0	35.0	41.0
20-21	39.010612689452664	40.0	39.0	41.0	35.0	41.0
22-23	38.98419474528323	40.0	39.0	41.0	35.0	41.0
24-25	38.96663505223958	40.0	38.0	41.0	35.0	41.0
26-27	38.868701902757564	40.0	38.0	41.0	35.0	41.0
28-29	38.78984573778191	40.0	38.0	41.0	35.0	41.0
30-31	38.68216126698128	40.0	38.0	41.0	35.0	41.0
32-33	38.554591409175075	40.0	38.0	41.0	34.5	41.0
34-35	38.44272396769978	40.0	38.0	41.0	34.0	41.0
36-37	38.30929464156338	40.0	38.0	41.0	34.0	41.0
38-39	38.204378224428	40.0	38.0	41.0	34.0	41.0
40-41	38.11295776033151	40.0	38.0	41.0	34.0	41.0
42-43	37.970029569515425	40.0	38.0	41.0	33.0	41.0
44-45	37.79522217498702	40.0	38.0	41.0	33.0	41.0
46-47	37.5475810014665	40.0	37.0	41.0	33.0	41.0
48-49	37.30186220986238	40.0	37.0	41.0	32.5	41.0
50-51	37.14005381927073	40.0	37.0	41.0	32.0	41.0
52-53	37.0236223638385	40.0	37.0	41.0	31.5	41.0
54-55	36.88225422360344	40.0	36.0	41.0	31.0	41.0
56-57	36.73390637253136	40.0	36.0	41.0	31.0	41.0
58-59	36.555647474343374	39.0	35.5	41.0	31.0	41.0
60-61	36.36846351873986	39.0	35.0	41.0	31.0	41.0
62-63	36.15591142104556	39.0	35.0	41.0	31.0	41.0
64-65	35.93816258269405	38.5	35.0	40.5	30.0	41.0
66-67	35.67949806207634	38.0	35.0	40.0	30.0	41.0
68-69	35.390258297549956	37.0	35.0	40.0	30.0	41.0
70-71	35.110557605192454	37.0	35.0	40.0	29.0	41.0
72-73	34.78352341939334	36.5	34.5	39.0	29.0	41.0
74-75	34.46106948519105	36.0	34.0	39.0	29.0	41.0
76-77	34.13894331934783	35.5	34.0	38.5	29.0	40.0
78-79	33.828878163111575	35.0	34.0	37.0	29.0	39.5
80-81	33.518360827265994	35.0	34.0	37.0	29.0	39.0
82-83	33.23784902613595	35.0	34.0	36.5	29.0	39.0
84-85	32.950222123181206	35.0	34.0	36.0	28.5	37.5
86-87	32.70108662286009	35.0	34.0	36.0	28.0	37.0
88-89	32.47794827986949	35.0	34.0	35.5	27.0	37.0
90-91	32.280336125331075	35.0	33.0	35.0	27.0	36.0
92-93	32.11404769257725	35.0	33.0	35.0	27.0	36.0
94-95	31.951093079254996	35.0	33.0	35.0	27.0	36.0
96-97	31.805511348795793	35.0	33.0	35.0	26.0	36.0
98-99	31.666117164314244	35.0	33.0	35.0	26.0	35.5
100	31.55516583501599	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2851079300329644
1101	2	0.3351472488177478
1101	3	0.4651672251873791
1101	4	0.2803351451032583
1101	5	0.2492331326578352
1101	6	0.2144072753034365
1101	7	0.24020140195880657
1101	8	0.2439992593389917
1101	9	0.3224359004701327
1101	10-11	0.306679725659599
1101	12-13	0.3093279922009984
1101	14-15	0.4261085982162527
1101	16-17	0.3787796809637314
1101	18-19	0.3901567976859184
1101	20-21	0.44175400329349657
1101	22-23	0.4462948239924671
1101	24-25	0.41844686190466973
1101	26-27	0.40459398069805985
1101	28-29	0.44125004514885546
1101	30-31	0.48733729130981374
1101	32-33	0.47336091103264977
1101	34-35	0.4514269423011399
1101	36-37	0.43734119723486486
1101	38-39	0.43150060369475085
1101	40-41	0.42852150925349974
1101	42-43	0.4119048882454308
1101	44-45	0.40526280362637124
1101	46-47	0.46634807763629027
1101	48-49	0.48927017992473054
1101	50-51	0.44542109379260353
1101	52-53	0.430007506141429
1101	54-55	0.393975779306011
1101	56-57	0.44865880491710186
1101	58-59	0.41956279090270954
1101	60-61	0.2877197290686766
1101	62-63	0.3848573311205783
1101	64-65	0.36806336953446106
1101	66-67	0.383278480021481
1101	68-69	0.391638451603896
1101	70-71	0.3917776526641745
1101	72-73	0.37221902983944233
1101	74-75	0.3458222004772509
1101	76-77	0.37947071015420875
1101	78-79	0.38852205806118434
1101	80-81	0.31931063265832904
1101	82-83	0.30802420568777933
1101	84-85	0.31532649102030064
1101	86-87	0.2683251019750088
1101	88-89	0.3011836005572732
1101	90-91	0.348029974041836
1101	92-93	0.3351577535966257
1101	94-95	0.32813606157965935
1101	96-97	0.3169660834136039
1101	98-99	0.34639516519995794
1101	100	0.3340930357112626
1102	1	0.27076323266056335
1102	2	0.2844415287826365
1102	3	0.4013841409558552
1102	4	0.23709115149711124
1102	5	0.232021210565037
1102	6	0.19452072655157338
1102	7	0.26287019117926036
1102	8	0.24259404434572218
1102	9	0.2976133281540143
1102	10-11	0.30197170593140754
1102	12-13	0.29328468800913043
1102	14-15	0.3430743530702287
1102	16-17	0.3740712910045403
1102	18-19	0.370691760170736
1102	20-21	0.40319723152695985
1102	22-23	0.324425166036022
1102	24-25	0.38948506158539686
1102	26-27	0.32337578354062657
1102	28-29	0.4179843201523781
1102	30-31	0.3707388198935675
1102	32-33	0.40386753330901115
1102	34-35	0.4334070917362425
1102	36-37	0.4418007659835723
1102	38-39	0.45339372000672284
1102	40-41	0.4811066971973048
1102	42-43	0.44658736801055454
1102	44-45	0.45222915812465914
1102	46-47	0.46268886386246066
1102	48-49	0.4233007777368556
1102	50-51	0.4220057370161925
1102	52-53	0.4223522008637488
1102	54-55	0.40787325712098976
1102	56-57	0.4337111896152237
1102	58-59	0.4272019878844091
1102	60-61	0.40504677611352946
1102	62-63	0.42059747640286105
1102	64-65	0.39290143959016177
1102	66-67	0.40920610742225705
1102	68-69	0.44116411554888657
1102	70-71	0.4209143319192208
1102	72-73	0.3689868677766839
1102	74-75	0.34469353504749023
1102	76-77	0.36527695011315586
1102	78-79	0.3565041567559817
1102	80-81	0.3485229234752154
1102	82-83	0.32143109495655153
1102	84-85	0.2309305297753994
1102	86-87	0.2697329670427706
1102	88-89	0.2810675215784073
1102	90-91	0.2943816515545805
1102	92-93	0.24334822842865123
1102	94-95	0.1951406893891665
1102	96-97	0.22315612223043502
1102	98-99	0.25812227486970585
1102	100	0.2870889501277816
1103	1	0.30093205379237276
1103	2	0.3111125295830348
1103	3	0.4106773250359055
1103	4	0.24604986141632423
1103	5	0.22076324782061363
1103	6	0.2277709661412075
1103	7	0.26163401669756325
1103	8	0.2329753295291752
1103	9	0.3134231222788628
1103	10-11	0.3007219243144732
1103	12-13	0.3006305715020261
1103	14-15	0.36111035999030605
1103	16-17	0.3910168380859531
1103	18-19	0.3687549451912133
1103	20-21	0.36346121936469444
1103	22-23	0.36499446503902533
1103	24-25	0.325611106343338
1103	26-27	0.3187636397874414
1103	28-29	0.37803149807836434
1103	30-31	0.3561367844395562
1103	32-33	0.3408650912339084
1103	34-35	0.3273275562532447
1103	36-37	0.35984863842467263
1103	38-39	0.3773826089044263
1103	40-41	0.3661397142885079
1103	42-43	0.3811429747052131
1103	44-45	0.3676963261945403
1103	46-47	0.3339509893869206
1103	48-49	0.3534775500931602
1103	50-51	0.32349228278625475
1103	52-53	0.3581074279608316
1103	54-55	0.3083660113121809
1103	56-57	0.3520693691522112
1103	58-59	0.3524461272658712
1103	60-61	0.2577245835287485
1103	62-63	0.33554768481685215
1103	64-65	0.3005647328929655
1103	66-67	0.30219278305778374
1103	68-69	0.3628948145738704
1103	70-71	0.30101095038321546
1103	72-73	0.3029530260076143
1103	74-75	0.304285861807422
1103	76-77	0.2931769334947347
1103	78-79	0.31957508539458956
1103	80-81	0.27179973866086016
1103	82-83	0.2772188245882603
1103	84-85	0.29706903562202314
1103	86-87	0.250217027779577
1103	88-89	0.2533355358399163
1103	90-91	0.2248293031401687
1103	92-93	0.2455321271829476
1103	94-95	0.23929238725740376
1103	96-97	0.28137100184896724
1103	98-99	0.3482186289948288
1103	100	0.32157610741997544
1104	1	0.3225035651331254
1104	2	0.2868672024774028
1104	3	0.3737725848013156
1104	4	0.2516141008819375
1104	5	0.23110375516353798
1104	6	0.19387660630854953
1104	7	0.1953495675928707
1104	8	0.22548544622572564
1104	9	0.2740307370861146
1104	10-11	0.30475209687198657
1104	12-13	0.2569563111533313
1104	14-15	0.3503649438990948
1104	16-17	0.34365878920251447
1104	18-19	0.3250801864567876
1104	20-21	0.3684004653122841
1104	22-23	0.2810410192035775
1104	24-25	0.3494619670685708
1104	26-27	0.3707152744416504
1104	28-29	0.3416442721036006
1104	30-31	0.29849689533465806
1104	32-33	0.30470797707531005
1104	34-35	0.35472193959309095
1104	36-37	0.3288731577452708
1104	38-39	0.31060240245074056
1104	40-41	0.3062855922693899
1104	42-43	0.3186650950223182
1104	44-45	0.3525715352703287
1104	46-47	0.27944331226483854
1104	48-49	0.31396592611200447
1104	50-51	0.3098484391566316
1104	52-53	0.3542264685341081
1104	54-55	0.33585896229853063
1104	56-57	0.319181623918837
1104	58-59	0.3313284198744526
1104	60-61	0.23219110134579068
1104	62-63	0.2410313507344597
1104	64-65	0.26621588086399584
1104	66-67	0.31424986142042144
1104	68-69	0.29686145101858585
1104	70-71	0.31446198992924934
1104	72-73	0.3275910098787591
1104	74-75	0.31046786226465173
1104	76-77	0.2500291702155266
1104	78-79	0.2768691588737866
1104	80-81	0.2928801798518563
1104	82-83	0.26283440348097287
1104	84-85	0.252807237816981
1104	86-87	0.26461046729549764
1104	88-89	0.2762950218020208
1104	90-91	0.28565261929588814
1104	92-93	0.3131555904431522
1104	94-95	0.29651595814044285
1104	96-97	0.3008702312140912
1104	98-99	0.36565518810930087
1104	100	0.32637475519029024
1105	1	0.22131293353194081
1105	2	0.2026969842345281
1105	3	0.3156687768276285
1105	4	0.18398444389906388
1105	5	0.17537109117026972
1105	6	0.18495685582166033
1105	7	0.1765059249515275
1105	8	0.15656422174860296
1105	9	0.166509909488461
1105	10-11	0.14743004359407053
1105	12-13	0.16730527300985898
1105	14-15	0.22590214902987782
1105	16-17	0.24155097409859394
1105	18-19	0.19769152778403054
1105	20-21	0.20868934221860513
1105	22-23	0.16180549659249266
1105	24-25	0.20039617019073575
1105	26-27	0.17770861632932622
1105	28-29	0.2276432068292209
1105	30-31	0.2193708971389654
1105	32-33	0.23777181911273715
1105	34-35	0.23196594964770867
1105	36-37	0.294052134706682
1105	38-39	0.29961826603808817
1105	40-41	0.29634278186544094
1105	42-43	0.27705233534807405
1105	44-45	0.24726327521099734
1105	46-47	0.27451046944194246
1105	48-49	0.2860535971364371
1105	50-51	0.225940479117142
1105	52-53	0.1982736008802135
1105	54-55	0.293736855976924
1105	56-57	0.2548554528366651
1105	58-59	0.34756068625556935
1105	60-61	0.2850868728833831
1105	62-63	0.29582070521315984
1105	64-65	0.3216043869658236
1105	66-67	0.28164652677725144
1105	68-69	0.36243413966273863
1105	70-71	0.3579149633461327
1105	72-73	0.38464426522746464
1105	74-75	0.34788735450836583
1105	76-77	0.3389025866429165
1105	78-79	0.32154356162249087
1105	80-81	0.313591086610586
1105	82-83	0.256570750272175
1105	84-85	0.23807951101047564
1105	86-87	0.20082521864402736
1105	88-89	0.2208316876719536
1105	90-91	0.2391966232916758
1105	92-93	0.23417129197777342
1105	94-95	0.18393956861940453
1105	96-97	0.19718372334895662
1105	98-99	0.259341748600729
1105	100	0.24385661731857766
1106	1	0.2064581720175127
1106	2	0.2280307507185313
1106	3	0.32277011516971044
1106	4	0.18565861098278447
1106	5	0.18125243505734545
1106	6	0.18064536641281848
1106	7	0.17315711028213343
1106	8	0.1396816983166005
1106	9	0.17152263689310843
1106	10-11	0.17469039903359374
1106	12-13	0.18328721565914918
1106	14-15	0.28623176812679674
1106	16-17	0.3034882052987129
1106	18-19	0.286541307454236
1106	20-21	0.27065563832653794
1106	22-23	0.2620515485372863
1106	24-25	0.26806564224619933
1106	26-27	0.2577334316342075
1106	28-29	0.2606625092041597
1106	30-31	0.29048850034106977
1106	32-33	0.28868760778220803
1106	34-35	0.25947266062190266
1106	36-37	0.326194684827513
1106	38-39	0.33455178444330613
1106	40-41	0.3228806862400475
1106	42-43	0.34057428382542554
1106	44-45	0.3097598598249647
1106	46-47	0.360941445969992
1106	48-49	0.32588889613773375
1106	50-51	0.34762963502242883
1106	52-53	0.35854213835064996
1106	54-55	0.29563917412364304
1106	56-57	0.3051514158296271
1106	58-59	0.2554813055422045
1106	60-61	0.28328391489589677
1106	62-63	0.2933058945049396
1106	64-65	0.2725097771362499
1106	66-67	0.267127516001473
1106	68-69	0.2653606619158495
1106	70-71	0.3235435503205082
1106	72-73	0.3398842143606515
1106	74-75	0.3213084054386428
1106	76-77	0.2989641544500259
1106	78-79	0.23276746801861492
1106	80-81	0.23204398106042845
1106	82-83	0.24344050480164015
1106	84-85	0.26306353759673584
1106	86-87	0.28602500720569424
1106	88-89	0.27476586401538583
1106	90-91	0.2811425707971864
1106	92-93	0.2786682844068551
1106	94-95	0.2606784931695465
1106	96-97	0.26516700140054184
1106	98-99	0.27605060879898957
1106	100	0.22983870455528077
1107	1	0.153371081074269
1107	2	0.17756511949222187
1107	3	0.24325131736421923
1107	4	0.12050397731577789
1107	5	0.1489948666935348
1107	6	0.16725588666552227
1107	7	0.14989033649275996
1107	8	0.13798409881508888
1107	9	0.14839325150717286
1107	10-11	0.14043860674519237
1107	12-13	0.1283652321460238
1107	14-15	0.17905440726121924
1107	16-17	0.18647095880637465
1107	18-19	0.18944019429250147
1107	20-21	0.2170995624816996
1107	22-23	0.22277357046409207
1107	24-25	0.17046567253309064
1107	26-27	0.19676118909148954
1107	28-29	0.19222585513062285
1107	30-31	0.21640354100485837
1107	32-33	0.13230307016166876
1107	34-35	0.17519667017862162
1107	36-37	0.17566990031968288
1107	38-39	0.13306896845940486
1107	40-41	0.12315447156237269
1107	42-43	0.115373383210013
1107	44-45	0.14124863996974568
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2216	72-73	-0.8924611673204623
2216	74-75	-0.8958890162745234
2216	76-77	-0.7849189626265698
2216	78-79	-0.8058947299516106
2216	80-81	-0.8168330493161875
2216	82-83	-0.8426497605188814
2216	84-85	-0.9473015267091967
2216	86-87	-0.8778016885434994
2216	88-89	-0.8762260829859656
2216	90-91	-0.7952313778560267
2216	92-93	-0.815623196854169
2216	94-95	-0.860265860645935
2216	96-97	-0.8753255856467135
2216	98-99	-1.0072545482938509
2216	100	-1.0166241699607355
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	392.0
3	52.0
4	69.0
5	128.0
6	441.0
7	1046.0
8	2312.0
9	3834.0
10	5181.0
11	6976.0
12	9692.0
13	13984.0
14	17380.0
15	22729.0
16	28351.0
17	40481.0
18	41051.0
19	21621.0
20	15319.0
21	14743.0
22	15454.0
23	16676.0
24	19362.0
25	24064.0
26	31040.0
27	39914.0
28	52643.0
29	68910.0
30	89980.0
31	116817.0
32	153113.0
33	200361.0
34	274792.0
35	403194.0
36	682027.0
37	1308239.0
38	1913744.0
39	807628.0
40	2717.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.78672079264763	21.01880562504345	15.506074773807166	22.688398808501756
2	15.295810364160776	21.111189635993867	39.93007299051088	23.662927009334478
3	17.7217446895572	25.862833387742313	31.26314765566368	25.152274267036802
4	12.653946357332927	18.143150105227637	38.640665205072885	30.562238332366547
5	12.640514475421133	37.75039188074143	35.72339212897768	13.885701514859761
6	29.58370196687548	38.80053797687108	17.76202054636169	13.85373950989175
7	25.769088080226933	33.36918501120474	22.92796503556739	17.933761873000932
8	24.46517466983852	36.06403011726515	20.719197545116284	18.751597667780054
9	24.55655392125858	16.945369001912486	22.189662747312784	36.30841432951615
10-11	23.325516585048042	27.37480509032999	29.042294721823716	20.257383602798257
12-13	24.27752941061852	25.004163794795204	29.267781414149972	21.450525380436304
14-15	21.516024926787576	27.019912140450327	27.18065704295258	24.28340588980952
16-17	20.403522361627086	30.171684432448863	27.887790794866497	21.537002411057554
18-19	20.232926624270448	28.41384393339351	30.95736196807618	20.39586747425986
20-21	21.32648527624942	27.56705874638925	30.77736386401394	20.329092113347386
22-23	21.00890023702315	27.490625855858937	30.811725802862373	20.688748104255545
24-25	20.878898599341184	27.597917994351466	30.755427585770693	20.767755820536657
26-27	20.663185419774692	27.627331319144314	30.818653862540184	20.89082939854081
28-29	20.64199143363978	27.59632515920233	30.99496370268912	20.76671970446877
30-31	21.002853649223987	27.84531776829259	30.578236273743105	20.573592308740317
32-33	20.64820039783764	27.71281862695445	30.91750242830038	20.721478546907527
34-35	20.897757458218617	27.478617734564693	30.81083659877426	20.812788208442427
36-37	21.14044058438802	27.72256121087637	30.349811341821344	20.787186862914268
38-39	20.967888598037536	27.795568732615095	30.42527770616893	20.811264963178445
40-41	20.938653114062305	27.615068034937835	30.457536484043736	20.988742366956124
42-43	20.993234780653456	27.6425328429463	30.280097741313366	21.08413463508688
44-45	21.11559699538712	27.821742261643433	30.019336709422177	21.04332403354727
46-47	21.280896169262395	27.881141094729305	29.673212085072244	21.164750650936053
48-49	21.267426660379865	27.8938683115035	29.377841683629846	21.46086334448679
50-51	21.303551542985595	27.969435194574093	29.192477426201087	21.534535836239225
52-53	21.31692826535458	28.12090917793159	29.0567462213079	21.50541633540593
54-55	21.37998443351591	28.341741080161825	28.945046723422113	21.333227762900147
56-57	21.296260069463077	28.044630931590515	29.00681934481278	21.65228965413363
58-59	21.265764235345568	27.773245843898753	29.261533788904803	21.699456131850873
60-61	21.318528832713184	28.06266244405553	28.99267713370707	21.626131589524217
62-63	21.187583865476874	27.99938204181981	29.18048476932577	21.632549323377546
64-65	21.22607480417793	27.820474179291686	29.179835263730975	21.773615752799408
66-67	21.122246695524304	27.85979246440516	29.118387395137706	21.899573444932827
68-69	21.429547896166323	27.950777373142664	28.967431469814148	21.652243260876862
70-71	21.40175833536046	27.784117330402104	28.945928195300763	21.868196138936668
72-73	21.211661965741055	27.892569300313912	29.041954504607393	21.853814229337644
74-75	21.33482224770243	27.905151762793118	28.84275955463398	21.917266434870474
76-77	21.35647851675191	27.92608069612154	28.70318321145567	22.014257575670882
78-79	21.404047069361166	27.92911172223058	28.826032555385428	21.840808653022822
80-81	21.20130430213874	27.878379570603425	28.878619810741498	22.041696316516344
82-83	21.12611410280876	27.914822651318026	28.986977944808356	21.972085301064855
84-85	21.119359609503206	27.724364736366493	28.905913224600994	22.25036242952931
86-87	21.224996810447813	27.88637461967006	28.743022612958473	22.145605956923646
88-89	21.095335595275284	27.7530225379731	28.759545467198233	22.392096399553385
90-91	20.979954691953065	27.692127351829864	28.8294214973677	22.498496458849367
92-93	21.0167336986295	27.830286900409305	28.72583340840037	22.427145992560824
94-95	20.84458823640392	27.609368120732874	28.90800364528185	22.63803999758136
96-97	20.833383915163562	27.72546692957362	28.78968801823018	22.651461137032637
98-99	20.797728992636763	27.714124197590262	28.7330711929075	22.755075616865476
100	20.87233012358711	27.643958022723748	28.674445998276333	22.809265855412807
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	728.0
1	555.5
2	592.5
3	848.0
4	597.0
5	425.5
6	1146.0
7	2973.0
8	5084.0
9	6116.5
10	5112.0
11	3587.5
12	3344.5
13	3795.0
14	4422.0
15	4993.0
16	5635.0
17	6477.0
18	7435.5
19	8530.5
20	10097.5
21	12424.0
22	16026.0
23	21524.0
24	28495.5
25	37757.5
26	50604.0
27	66223.5
28	85931.5
29	107123.5
30	126287.0
31	145389.0
32	163496.5
33	178981.5
34	192308.0
35	203796.0
36	213511.0
37	222510.5
38	230484.5
39	233202.5
40	233454.5
41	232692.5
42	228837.0
43	225093.5
44	219681.0
45	214036.5
46	209397.0
47	202883.5
48	196819.0
49	192655.0
50	187824.5
51	178593.0
52	166629.5
53	158570.5
54	153042.5
55	145357.0
56	133443.0
57	118565.0
58	106326.5
59	97295.5
60	85929.5
61	72599.5
62	61302.0
63	50815.5
64	40821.0
65	32852.5
66	26275.5
67	20332.5
68	15444.5
69	11573.5
70	8446.5
71	6038.5
72	4578.0
73	3392.0
74	2343.0
75	1582.0
76	965.0
77	692.0
78	497.5
79	283.5
80	146.0
81	58.0
82	32.5
83	20.5
84	9.0
85	4.5
86	3.5
87	4.0
88	3.0
89	1.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11904509687453269
2	0.0
3	0.0
4	0.0
5	0.013021040733743376
6	1.2371535138948578E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	7.732209461842861E-6
76-77	0.0
78-79	0.0
80-81	1.6237639869870008E-4
82-83	6.64970013718486E-4
84-85	4.2527152040135733E-4
86-87	4.948614055579431E-4
88-89	5.721835001763718E-4
90-91	8.660074597264004E-4
92-93	2.2423407439344295E-4
94-95	2.4743070277897155E-4
96-97	5.799157096382146E-4
98-99	0.0011675636287382719
100	9.278651354211433E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6466457.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.06693077398989
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.42130274064918	43.958379370920696
2	15.373070445533182	18.160801755799564
3	4.778943227615353	8.468325263951515
4	2.041478672692838	4.823355177460986
5	0.9702529820696205	2.8654932862581766
6	0.5944787982991336	2.106842281544377
7	0.37769411043073553	1.5616462312189165
8	0.25534783761258417	1.2066090438040418
9	0.17825068820115786	0.9475848954354462
>10	0.930700883668168	9.606918727100888
>50	0.051843263948872814	2.10737700992727
>100	0.025012391316454875	2.665353723453506
>500	0.0011001235086025459	0.43111223349261846
>1k	4.1906756386383406E-4	0.33405767460642816
>5k	5.238344513313429E-5	0.22362819713652973
>10k+	5.238344513313429E-5	0.5325151278890689
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17418	0.2693592488127579	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17003	0.2629415149594283	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9136	0.14128293128679276	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.5464418923685722E-5	0.0
5	0.0	0.0	0.0	1.5464418923685722E-5	0.0
6	0.0	0.0	0.0	1.5464418923685722E-5	0.0
7	0.0	0.0	0.0	1.5464418923685722E-5	0.0
8	0.0	0.0	0.0	1.5464418923685722E-5	0.0
9	0.0	0.0	0.0	7.732209461842861E-5	0.0
10-11	0.0	0.0	0.0	7.732209461842861E-5	0.0
12-13	1.5464418923685722E-5	7.732209461842861E-6	0.0	1.0825093246580005E-4	0.0
14-15	1.5464418923685722E-5	1.5464418923685722E-5	0.0	1.391797703131715E-4	0.0
16-17	1.5464418923685722E-5	1.5464418923685722E-5	0.0	1.5464418923685723E-4	0.0
18-19	1.5464418923685722E-5	3.0928837847371444E-5	0.0	1.8557302708422865E-4	0.0
20-21	1.5464418923685722E-5	3.0928837847371444E-5	0.0	2.165018649316001E-4	0.0
22-23	3.86610473092143E-5	3.0928837847371444E-5	0.0	3.634138447066145E-4	0.0
24-25	4.639325677105716E-5	3.0928837847371444E-5	0.0	6.185767569474288E-4	0.0
26-27	4.639325677105716E-5	3.0928837847371444E-5	0.0	0.0011675636287382719	0.0
28-29	4.639325677105716E-5	3.0928837847371444E-5	0.0	0.0033171178591305875	0.0
30-31	6.185767569474289E-5	3.0928837847371444E-5	0.0	0.00907761390820352	0.0
32-33	7.732209461842861E-5	3.0928837847371444E-5	0.0	0.022740428027279852	0.0
34-35	7.732209461842861E-5	3.0928837847371444E-5	0.0	0.04667934852114535	0.0
36-37	7.732209461842861E-5	3.0928837847371444E-5	0.0	0.0837243640528345	0.0
38-39	7.732209461842861E-5	3.0928837847371444E-5	0.0	0.1319114934190392	0.0
40-41	7.732209461842861E-5	3.0928837847371444E-5	0.0	0.181768780029002	0.0
42-43	7.732209461842861E-5	3.0928837847371444E-5	7.732209461842861E-6	0.23224464339591216	0.0
44-45	7.732209461842861E-5	3.0928837847371444E-5	1.5464418923685722E-5	0.29978549304510954	0.0
46-47	7.732209461842861E-5	3.0928837847371444E-5	1.5464418923685722E-5	0.38486299375376654	0.0
48-49	7.732209461842861E-5	3.0928837847371444E-5	3.0928837847371444E-5	0.47452569467329636	0.0
50-51	9.278651354211433E-5	4.639325677105716E-5	3.0928837847371444E-5	0.5640337514035894	0.0
52-53	1.0825093246580005E-4	6.185767569474289E-5	3.0928837847371444E-5	0.6538588287218179	0.0
54-55	1.2371535138948578E-4	6.958988515658575E-5	3.0928837847371444E-5	0.7586611957676359	0.0
56-57	1.2371535138948578E-4	7.732209461842861E-5	3.0928837847371444E-5	0.8863354384015854	0.0
58-59	1.2371535138948578E-4	7.732209461842861E-5	3.0928837847371444E-5	1.0291261505334375	0.0
60-61	1.2371535138948578E-4	7.732209461842861E-5	3.0928837847371444E-5	1.1762855610112308	0.0
62-63	1.2371535138948578E-4	7.732209461842861E-5	3.0928837847371444E-5	1.3262826923615203	0.0
64-65	1.5464418923685723E-4	7.732209461842861E-5	3.0928837847371444E-5	1.4915509373989497	0.0
66-67	1.5464418923685723E-4	7.732209461842861E-5	3.0928837847371444E-5	1.683394786356733	0.0
68-69	1.5464418923685723E-4	7.732209461842861E-5	3.0928837847371444E-5	1.8992703423219237	0.0
70-71	1.5464418923685723E-4	7.732209461842861E-5	3.0928837847371444E-5	2.130865480123041	0.0
72-73	1.5464418923685723E-4	7.732209461842861E-5	3.0928837847371444E-5	2.3737419115289873	0.0
74-75	1.5464418923685723E-4	7.732209461842861E-5	3.0928837847371444E-5	2.6217447978081347	0.0
76-77	1.7784081762238578E-4	7.732209461842861E-5	3.0928837847371444E-5	2.894359306804329	0.0
78-79	1.8557302708422865E-4	7.732209461842861E-5	3.0928837847371444E-5	3.199163003790175	0.0
80-81	1.8557302708422865E-4	7.732209461842861E-5	3.0928837847371444E-5	3.529985585615121	0.0
82-83	1.8557302708422865E-4	7.732209461842861E-5	3.0928837847371444E-5	3.8742080864374415	0.0
84-85	1.8557302708422865E-4	7.732209461842861E-5	3.0928837847371444E-5	4.225961759275597	0.0
86-87	1.8557302708422865E-4	7.732209461842861E-5	3.0928837847371444E-5	4.600525450026189	0.0
88	1.8557302708422865E-4	7.732209461842861E-5	3.0928837847371444E-5	4.904370353038766	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11415	0.0	56.683636	1
GTATCAA	20760	0.0	40.234806	1
ATCAACG	25185	0.0	32.772743	3
TCAACGC	25605	0.0	32.271885	4
TATCAAC	26445	0.0	31.317894	2
GTGGTAT	4895	0.0	31.051367	1
CAACGCA	26905	0.0	30.730036	5
AACGCAG	28105	0.0	29.49861	6
TGGTATC	4985	0.0	28.946991	2
ACGCAGA	31645	0.0	26.065052	7
CGCAGAG	31570	0.0	25.963215	8
GCAGAGT	35070	0.0	23.157654	9
GAGTACT	24975	0.0	21.123575	12-13
GTACATG	17430	0.0	21.11253	1
CAGAGTA	34190	0.0	20.743244	10-11
AGAGTAC	32520	0.0	20.384924	10-11
TACATGG	17435	0.0	19.949867	2
ACATGGG	17445	0.0	19.642048	3
GTACTTT	27445	0.0	19.17968	14-15
AGTACTT	26420	0.0	18.811964	12-13
>>END_MODULE